Science Quiz for Biochemistry,Microbiology students and Medical Laboratory Technology Students

Science Quiz

Competition By

Sachin Narwadiya

Scientist

Vigyan Prasar

sachin@vigyanprasar.gov.in

Round I

GeneralTerms and conditions

• Each team will get two questions with multiple choice.

• If unanswered, question will not be PASSED ON to the next team.

• Each question will carry 10 points.

• Answer time is 10 seconds.

TEAM- A

Team A:Hormone that act on the same cell

that released them is called as…………….

a) Autocrine

b) Paracrine

c) Endocrine

d) Self Enhancing

TEAM- B

Arterial blood specifically collected for the…...

a) Blood culture b) Blood gas analysis

c) Blood smears

d) Serum proteins

TEAM- C

Albumin, a plasma protein has half life of …

a) 2 days

d) 120 days

b) 20 days

c) 200 days

TEAM- D

Which bacterial species can oxidize the reagent tetramethyl-phenylenediamine dihydrochloride to produce a colour change

a) Pseudomonas

b) E.coli

c) C.Diptheria

d) M.Tuberculosis

TEAM- E

The infection acquired in hospitals are known as…..

a) Primary Infection

b) Sub-cuteneous Infection

c) Nosocomial Infection

d) Endemic diseases

TEAM- A

a) Not changed

b) Inverted

c) Denominator increas sunstatially

d) Numerator decreases

The CD4+ to CD8+ T cells ratio in healthy adult is 1.5:1 which is ………..in HIV patients.

TEAM- B

Hyponatremia is condition of

a) Low sodium in blood

d) High Potassium in blood

b) High Sodium in blood

c) Low Potassium in blood

TEAM- C

Electrode developed for measurement of physiologically important compounds are termed as…………..

a) Ion selective

d) Cathod

b) Biosensors

c) pH Meter

TEAM- D

The dielectric constant for a vacuum is The dielectric constant for a vacuum is one; for hexane it is 1.9; for ethanol it one; for hexane it is 1.9; for ethanol it is 24.3; and for water it is……………is 24.3; and for water it is……………

a) 100.5

b) 78.5

c) 88.5

d) 60.5

TEAM- E

β-amyloid, a 4.3-kDa polypeptide produced β-amyloid, a 4.3-kDa polypeptide produced by proteolytic cleavage of a larger protein by proteolytic cleavage of a larger protein known as amyloid precursor protein. In known as amyloid precursor protein. In which disease β-amyloid level elevated?which disease β-amyloid level elevated?

a) Alzheimer’s disease

b) Cushing’s Syndrom

c) AIDS

d) TB

Round II

Laboratory Based Questions

Terms and conditions• Each team will get two questions with 10 point to each question.

• If unanswered, question will PASS ON to next team carrying 5 bonus point.

• Answer time is 30 seconds.

TEAM- A

VDRL test is used to detect………….infection

Syphilis

TEAM- B

Albert Staining is used for detection of

Corynbacterium diptheria

TEAM- C

Taq polymerase used in PCR extracted from the bacteria …………….

Thermococcus aquaticusThermococcus aquaticus

TEAM- D

Infectious mononucleosis is caused by theInfectious mononucleosis is caused by the

Epstein-Barr virus (EBV)Epstein-Barr virus (EBV)

TEAM- E

Which are immature erythrocytes that pass Which are immature erythrocytes that pass into the bloodstream from the bone into the bloodstream from the bone marrow?marrow?

Reticulocytes

TEAM- A

The toxin produced by haemolytic The toxin produced by haemolytic streptococci is……………..streptococci is……………..

Streptolysin O

TEAM- B

DNA has equal numbers of adenine and DNA has equal numbers of adenine and thymine residues (A=T) and equal thymine residues (A=T) and equal numbers of guanine and cytosine numbers of guanine and cytosine residues (G=C)residues (G=C). These relationships, . These relationships, known asknown as

Chargaff’s Rules

TEAM- C

In routine urine examination sulphur powder test is for the presence of____

Bile saltsBile salts

TEAM- D

Spirometry is the test associated with ____ organ.

LungsLungs

TEAM- E

Name the technique that employs isotopically labeled molecules and permits measurements of extremely small amounts of antigen-antibody complexes.

Radioimmunoassay (RIA)

Round III

Photo Identification Round

Terms and conditions •Each team will get a question.

•Picture of equipment/process/technique will be shown on the screen.

•If identified without any clue then 10 points will be awarded.

• 2 points for each clue will be deducted for next four clues.

• If unanswered, question WILL NOT pass on to next team.

TEAM-A

1. Individual cells carried in thin film

4. It is basic of cell counters

2. Cells are passed through laser film

3. It is used to distinguish between different cells

Flow Cytometry

TEAM-B

1. Its separation technique

2. Has two phases stationary phase and mobile phase

Column Chromatography

3. It can be performed using paper, column.

4. Eluted fractions can be evaluated by using spectroscopy.

TEAM-C

2. Its postulates were famous in microbiology

4. Richerd Petri was his assistant.

1.He introduced use of solid media for culture

3.He created methods for the cultivation, isolation & identification of bacteria.

Robert Koch 1843-1910

TEAM-D

X-Ray Crystallography

1.Technique is used to determine 3d structure of proteins

2.Max Perutz and John Kenrew in 1950 invented this technique.

3. Atoms in crystals scatters the X-rays

4.Till date about 10,000 proteins structure has been determined by this technique.

TEAM-E

Fluorescent Activated cell sorter ( FACS)

4. It can be used to purify different types WBCs

3. It can select one cells from thousand of other cells

2.It is based on flow cytometry

1.General shapes of DNA &RNA contents can be determined.

• Each team will be asked continuous questions for 1 minute or maximum eight question.

• Answer should be given in True or False.

• Each question will carry 5 points.

Terms and conditions

Round IV

Science in General

1. Ebola virus belongs to Filoviridae family

2. M.Tubercule bacteria also called as acid fast bacilli

3. FSH secreted by Ovary.4. Detoxification is work of heart….5. RBCs are nucleated……6. PCR generates same copies of DNA strands

7. Genes are bigger that DNA in size8. RBCs life span is about 120 days09. After age of 70 years red bone marrow decreases in bones.

10. Presbiopia disease associated with lungs.11.Flourides has capacity to stop glycolysis.

12.Urea Cycle operates in Lungs

13 Lipids in diet are mainly triacylglycerols.14. In blood T3 and T4 bound to albumin.15. 0.85% NaCl is the concentration in Normal Saline16. 40g NaCl in 1 Litre water constitutes 1 Molar solution.

17.Human histocompatibility antigens are also known as human leucocyte antigens (HLA), a term that is synonymous with the MHC complex.

19. Wintrob Method is used for ESR estimation20. Atomizer is used in flame photometry21. Sickle cells anemia has spheroid RBCs22.Polychromatophilia means the erythrocytes have a blue-gray hue to the color of their cytoplasm

23. DOTS program associated with treatment of HIV-AIDS

24. Diseases spread world wide are pandemic diseases

18. Western Blotting separates DNA from RNA

25. Buffer has capacity to resists in temperature

26.EDTA anticoagulant is unsuitable for serum calcium analysis.

27.Salivary alpha-amylase acts on starch

28. Conjugation of Bilirubin with acids makes it soluble in water

29. The Ehrlich reaction is based on the coupling of diazotized sulfanilic acid and Bilirubin to produce a reddish-purple azo compound

30. Tourniquet test associated with reduced platelet counts

33. Plasma can be obtained from clotted blood.

34. B Lymphocytes synthesis Immunoglobulin.

35. Macconkeys agar is an indicator media.

36. In blood group O, Ag A & B present on RBC surface.

31. Iodine acts as decolourizer in grams stain

32. Hyaluronic acid present in synovial fluid.

37. RBCs swells in isotonic solutions.

38. Study of color is called chromatography.39. Bense Johns Protein appear in urine in myloma condition

40. Ketone bodies in urine can be detected by Rothra’s Test.