Molbiol 2011-03-biochem

Биологические макромолекулы

Белки

Углеводы

Липиды

Нуклеиновые кислоты

Organic Compounds

Molecules unique to living systems contain carbon and hence are organic compounds

They include:

Carbohydrates

Lipids

Proteins

Nucleic Acids

Carbohydrates

Contain carbon, hydrogen, and oxygen

Their major function is to supply a source of cellular food

Examples:

Monosaccharides or simple sugars

Figure 2.14a

Carbohydrates

Disaccharides or double sugars

Figure 2.14b

PLAYPLAY Disaccharides

Carbohydrates

Polysaccharides or polymers of simple sugars

Figure 2.14c

PLAYPLAY Polysaccharides

Lipids

Contain C, H, and O, but the proportion of oxygen in lipids is less than in carbohydrates

Examples:

Neutral fats or triglycerides

Phospholipids

Steroids

Eicosanoids

PLAYPLAY Fats

Neutral Fats (Triglycerides)

Composed of three fatty acids bonded to a glycerol molecule

Figure 2.15a

Other Lipids

Phospholipids – modified triglycerides with two fatty acid groups and a phosphorus group

Figure 2.15b

Other Lipids

Steroids – flat molecules with four interlocking hydrocarbon rings

Eicosanoids – 20-carbon fatty acids found in cell membranes

Figure 2.15c

Representative Lipids Found in the Body

Neutral fats – found in subcutaneous tissue and around organs

Phospholipids – chief component of cell membranes

Steroids – cholesterol, bile salts, vitamin D, sex hormones, and adrenal cortical hormones

Representative Lipids Found in the Body

Fat-soluble vitamins – vitamins A, E, and K

Eicosanoids – prostaglandins, leukotrienes, and thromboxanes

Lipoproteins – transport fatty acids and cholesterol in the bloodstream

Amino Acids

Building blocks of protein, containing an amino group and a carboxyl group

Amino group NH2

Carboxyl groups COOH

Amino Acids

Figure 2.16a–c

Amino Acids

Figure 2.16d, e

Protein

Macromolecules composed of combinations of 20 types of amino acids bound together with peptide bonds

Figure 2.17

Protein

Figure 2.17

Amino acid Amino acid

Dehydrationsynthesis

HydrolysisDipeptide

Peptide bond

OH OH OH

Protein

Figure 2.17

Protein

Figure 2.17

Protein

Figure 2.17

Dipeptide

Peptide bond

OH OH OH

Protein

Figure 2.17

Dipeptide

Peptide bond

Protein

Figure 2.17

HydrolysisDipeptide

Peptide bond

Protein

Figure 2.17

Amino acid Amino acidHydrolysis

Dipeptide

Peptide bond

OH OH OH

Protein

Figure 2.17

HydrolysisDipeptide

Peptide bond

OH OH OH

Structural Levels of Proteins

Primary – amino acid sequence

Secondary – alpha helices or beta pleated sheets

PLAYPLAY Chemistry of Life: Proteins: Secondary Structure

PLAYPLAY Chemistry of Life: Proteins: Primary Structure

PLAYPLAY Chemistry of Life: Introduction to Protein Structure

Tertiary – superimposed folding of secondary structures

Quaternary – polypeptide chains linked together in a specific manner

PLAYPLAY Chemistry of Life: Proteins: Quaternary Structure

PLAYPLAY Chemistry of Life: Proteins: Tertiary Structure

Figure 2.18a–c

Figure 2.18b,d,e

Fibrous and Globular Proteins

Fibrous proteins

Extended and strand-like proteins

Examples: keratin, elastin, collagen, and certain contractile fibers

Fibrous and Globular Proteins

Globular proteins

Compact, spherical proteins with tertiary and quaternary structures

Examples: antibodies, hormones, and enzymes

Protein Denuaturation

Reversible unfolding of proteins due to drops in pH and/or increased temperature

Figure 2.19a

Protein Denuaturation

Irreversibly denatured proteins cannot refold and are formed by extreme pH or temperature changes

Figure 2.19b

Molecular Chaperones (Chaperonins)

Help other proteins to achieve their functional three-dimensional shape

Maintain folding integrity

Assist in translocation of proteins across membranes

Promote the breakdown of damaged or denatured proteins

Characteristics of Enzymes

Most are globular proteins that act as biological catalysts

Holoenzymes consist of an apoenzyme (protein) and a cofactor (usually an ion)

Enzymes are chemically specific

Frequently named for the type of reaction they catalyze

Enzyme names usually end in -ase

Lower activation energy

Figure 2.20

Mechanism of Enzyme Action

Enzyme binds with substrate

Product is formed at a lower activation energy

Product is released

PLAYPLAY How Enzymes Work

Active siteAmino acids

Enzyme (E)Enzyme-substratecomplex (E-S)

Internal rearrangementsleading to catalysis

Dipeptide product (P)

Free enzyme (E)

Substrates (S)

Peptide bond

Substrates (S)

Dipeptide product (P)

Free enzyme (E)

Substrates (S)

Peptide bond

Nucleic Acids

Composed of carbon, oxygen, hydrogen, nitrogen, and phosphorus

Their structural unit, the nucleotide, is composed of N-containing base, a pentose sugar, and a phosphate group

Nucleic Acids

Five nitrogen bases contribute to nucleotide structure – adenine (A), guanine (G), cytosine (C), thymine (T), and uracil (U)

Two major classes – DNA and RNA

Deoxyribonucleic Acid (DNA)

Double-stranded helical molecule found in the nucleus of the cell

Replicates itself before the cell divides, ensuring genetic continuity

Provides instructions for protein synthesis

Structure of DNA

Figure 2.22a

Structure of DNA

Figure 2.22b

Ribonucleic Acid (RNA)

Single-stranded molecule found in both the nucleus and the cytoplasm of a cell

Uses the nitrogenous base uracil instead of thymine

Three varieties of RNA: messenger RNA, transfer RNA, and ribosomal RNA

Adenosine Triphosphate (ATP)

Source of immediately usable energy for the cell

Adenine-containing RNA nucleotide with three phosphate groups

Adenosine Triphosphate (ATP)

Figure 2.23

Solute Solute transported

Contracted smoothmuscle cell

Product made

Relaxed smoothmuscle cell

Reactants

Membraneprotein

(a) Transport work

(b) Mechanical work

(c) Chemical work

Solute

Membraneprotein

(a) Transport work

Membraneprotein

(a) Transport work

(b) Mechanical work

Reactants

(c) Chemical work

Product madeReactants

(c) Chemical work

Product made

Reactants

Membraneprotein

(a) Transport work

(b) Mechanical work

(c) Chemical work

МЕТАБОЛИЗМ

Catabolism provides the building blocks and energy for anabolism.

Figure 5.1

A metabolic pathway is a sequence of enzymatically catalyzed chemical reactions in a cell.

Metabolic pathways are determined by enzymes.

Enzymes are encoded by genes.

PLAY Animation: Metabolic Pathways (Overview)

Oxidation-Reduction

Oxidation is the removal of electrons.

Reduction is the gain of electrons.

Redox reaction is an oxidation reaction paired with a reduction reaction.

Figure 5.9

Oxidation-Reduction

In biological systems, the electrons are often associated with hydrogen atoms. Biological oxidations are often dehydrogenations.

Figure 5.10

The Generation of ATP

ATP is generated by the phosphorylation of ADP.

Substrate-level phosphorylation is the transfer of a high-energy PO4

– to ADP.

Energy released from the transfer of electrons (oxidation) of one compound to another (reduction) is used to generate ATP by chemiosmosis.

Light causes chlorophyll to give up electrons. Energy released from the transfer of electrons (oxidation) of chlorophyll through a system of carrier molecules is used to generate ATP.

Molbiol 2011-03-biochem

Technology

MolBiol #1

MolBiol Recombination

Molbiol 2011-01-chemistry

Biochem textbook

Biochemistry & Ariyurek et al., Biochem Anal Biochem … · Biochem Anal Biochem ISSN:2161-1009 Biochem, an open access journal . Keywords: ... turbidimetry and nephelometry in each

MolBiol #3.1

Molbiol 2011-10-proteins

Molbiol 2011-05-dna-rna-protein

MolBiol #2

Molbiol 2011-06-transcription-translation

Biochem mutations

MolBiol 07

Molbiol 2011-03-biochem

Biochem 5ed

Biochem Ppt

Biochem 03 Cell Communication November 10, 2010

Biochem Carbohydrates

Proteins biochem

MolBiol 02 DNA RNA Chromosome

Materials Background Materials From Bi 150 Molbiol