Plant tissue culture

Plant Tissue Culture ProjectOriginal Power Point Presentation by Paige and Original Power Point Presentation by Paige and

ElisaElisa

Modified by GA Agricultural Education Curriculum OfficeModified by GA Agricultural Education Curriculum Office

July 2002July 2002

What is Plant Tissue Culture?What is Plant Tissue Culture?

Plant tissue culture is a form of Plant tissue culture is a form of asexual propagation of plants asexual propagation of plants under laboratory conditions.under laboratory conditions.

Why is Plant Tissue Culture Important?Why is Plant Tissue Culture Important?

It has made significant It has made significant contributions in:contributions in: The production of plant materialThe production of plant material Plant breedingPlant breeding Gene banksGene banks The production of chemical The production of chemical

compoundscompounds

How is Plant Tissue Culture Done?How is Plant Tissue Culture Done?

It is grown on a special culture It is grown on a special culture medium which supports its growth medium which supports its growth and development.and development.

The medium can be either The medium can be either semisolid, such as agar, or liquid, semisolid, such as agar, or liquid, such as purified water.such as purified water.

Microorganisms and Plant Tissue CultureMicroorganisms and Plant Tissue Culture

In the practice of plant tissue culture, In the practice of plant tissue culture, microorganisms are called “contaminants” microorganisms are called “contaminants” because of their harmful effects on plant because of their harmful effects on plant growth in vitro.growth in vitro.

Six potential sources of contamination in the Six potential sources of contamination in the plant tissue culture lab are:plant tissue culture lab are: AirAir WaterWater Growth MediaGrowth Media PeoplePeople EquipmentEquipment Plant MaterialPlant Material

Experiment One: DescriptionExperiment One: Description

A one dollar bill was cut in half and A one dollar bill was cut in half and placed in two different petri dishes.placed in two different petri dishes.

Dollar 1 was placed in a room at Dollar 1 was placed in a room at 100100 F. F.

Dollar 2 was placed in a room at Dollar 2 was placed in a room at room temperature.room temperature.

Experiment One: resultsExperiment One: results

Day 1: Dollar 1Day 1: Dollar 1

No visible change.No visible change.

Day 2: Dollar 1Day 2: Dollar 1

Bacteria begin to Bacteria begin to develop around develop around the edges of the the edges of the dish.dish.

Day 3: Dollar 1Day 3: Dollar 1

Bacteria develop Bacteria develop rapidly covering rapidly covering most of the dish.most of the dish.

Day 6: Dollar 1Day 6: Dollar 1

Bacteria cover the Bacteria cover the entire dollar and entire dollar and dish.dish.

Day 7: Dollar 1Day 7: Dollar 1

The bacteria The bacteria continue to continue to spread, and the spread, and the nutrient agar has nutrient agar has begun to dissolve. begun to dissolve.

Day 1: Dollar 2Day 1: Dollar 2

No visible change.No visible change.

Day 2: Dollar 2Day 2: Dollar 2

Bacteria begin to Bacteria begin to develop in the develop in the middle of the middle of the dollar; although dollar; although development is development is not as rapid as in not as rapid as in Dollar 1.Dollar 1.

Day 3: Dollar 2Day 3: Dollar 2

The bacteria have The bacteria have spread to the spread to the edges of the edges of the dollar.dollar.

Day 6: Dollar 2Day 6: Dollar 2

The bacteria The bacteria cover the majority cover the majority of the bill.of the bill.

Day 7: Dollar 2Day 7: Dollar 2

The entire dollar The entire dollar is covered with is covered with bacteria.bacteria.

ResultsResults

The bacteria on dollar 1 developed at The bacteria on dollar 1 developed at a more rapid pace; however, the agar a more rapid pace; however, the agar dissolved due to the high dissolved due to the high temperatures.temperatures.

The bacteria on dollar 2 developed at The bacteria on dollar 2 developed at a slower pace in the beginning of the a slower pace in the beginning of the experiment, but dollar 2 contained experiment, but dollar 2 contained more bacteria than dollar 1 at the more bacteria than dollar 1 at the conclusion.conclusion.

Experiment 2: DescriptionExperiment 2: Description

A throat culture was taken from A throat culture was taken from two different people at the same two different people at the same time.time.

Mouth 1 was taken from a person Mouth 1 was taken from a person with flu-like symptoms.with flu-like symptoms.

Mouth 2 was taken from a healthy Mouth 2 was taken from a healthy person.person.

Experiment two: resultsExperiment two: results

Day 1: Mouth 1Day 1: Mouth 1

The bacteria have The bacteria have grown slightly and grown slightly and exhibit a faint exhibit a faint yellowish color.yellowish color.

Day 2: Mouth 1Day 2: Mouth 1

The bacteria have The bacteria have grown grown considerably and considerably and can be seen with can be seen with the naked eye.the naked eye.

Day 3: Mouth 1Day 3: Mouth 1

The bacteria are The bacteria are spreading.spreading.

Day 6: Mouth 1Day 6: Mouth 1

The bacteria have The bacteria have grown grown considerably: considerably: yellowish colored yellowish colored spots are spots are beginning to beginning to form.form.

Day 7: Mouth 1Day 7: Mouth 1

The bacteria are The bacteria are still growing at a still growing at a rapid pace and rapid pace and can now be seen can now be seen easily without a easily without a microscope.microscope.

Day 1: Mouth 2Day 1: Mouth 2

Very little Very little bacteria have bacteria have formed.formed.

Day 2: Mouth 2Day 2: Mouth 2

The bacteria The bacteria begin to grow but begin to grow but cannot be seen cannot be seen without a without a microscope.microscope.

Day 3: Mouth 2Day 3: Mouth 2

The bacteria are The bacteria are now growing now growing rapidly, and small rapidly, and small yellowish spots yellowish spots slightly visible to slightly visible to the naked eye are the naked eye are beginning to beginning to form.form.

Day 6: Mouth 2Day 6: Mouth 2

The bacteria have The bacteria have grown gradually, grown gradually, and are easier to and are easier to see with the see with the naked eye.naked eye.

Day 7: Mouth 2Day 7: Mouth 2

The bacteria are The bacteria are growing slowly, growing slowly, but the small, but the small, yellowish spots yellowish spots appear clearly.appear clearly.

ResultsResults

The mouth 1 culture grew a larger The mouth 1 culture grew a larger amount of bacteria at a faster rate amount of bacteria at a faster rate than the mouth 2 culture.than the mouth 2 culture.

Mouth 2, however, did begin to Mouth 2, however, did begin to grow towards the end of the grow towards the end of the experiment.experiment.