BACTERIAL METABOLISM All chemical processes present in

bacterial cell Divided into: Anabolism processes: synthesis of cell

components Catabolism processes: breakdown cell

components for energy generation Vary, depend on nutrition Pathogen bacteria : heterotrophic Enzymes play role in metabolism

METABOLISM ENERGY USAGE: Grow Replication & multiplication Replace a new components cell Heat Luminesention Fermentation process Respiration process Photosynthesis process

ENZYMES in metabolism: Dehydrogenises reduction &

oxidation Flavoprotein electron transfer Iron Porphyrin Protein Enzyme

respiration Example : - catalase - peroxidase - cytochrom oxidase

CARBOHYDRATE METABOLISM

Prominent source of carbon for both aerobe & anaerobe bacterial

Source of energies For amino acid, lipid, carbohydrate,

purine etc Cleavage of glycoside binding by

hydrolysis, phosphorilation, transglycosidation

Carbohydrate --------Thriose (Phosphate &

Pyruvate) Enzymes:

glycosidase phosphorilase

Example: Glucose pyruvic acid (Embden-Meyerhoff Cycle)

EMBDEN MEYERHOFF

CYCLE:

Glucose

Glucose 6 phosphate

Phosphooglyseraldehide

Phosphooenol pyruvate

Pyruvate

PENTOSA PHOSPHATE

CYCLE

Glucose

Glucose 6 phosphate

Pentose phosphate

ENTNER DOUDOROFF

CYCLE

Glucose

Glucose 6 phosphate ketodeoxy gluconate

piruvate +

gliseraldehide

PIRUVAT FERMENTATION

PRODUCES:

Alcohol lactate acid butyric acid propionic acid acetic acid

LIPID METABOLISM

Similar to carbohydrate metabolism

Starting with lipid acid activation by CoA glyserol+acetylCoA

Acetyl-coA Krebs's Cycle

AMINO ACIDS METABOLISMAmino acid was metabolized through: deamination: R--C--C--C = O R--C--COOH + NH2 NH2 OH transamination decarboxilation: R--CH----COOH R----CH2NH2 + CO2 NH2 decarboxilase

TQ