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Supplemental Figure 3. (A) ATG5. (B) AIM1. CpG island (-980 to -1360). CpG island (+30 to +380). CpG island (+610 to +970). (-2110 to -3140). (-477 to- 751). CpG island (+ 568 to -110). SINE. Set 1 2 3. LTR. Methylation Specific PCR. - PowerPoint PPT Presentation
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(-477
to-
751
)(A) ATG5
(-211
0 to
-314
0)
LT
RCpG
isla
nd
(+ 5
68 t
o -1
10)
S
INE
Supplemental Figure 3
(B) AIM1
CpG
isla
nd
(-980
to
-136
0)
CpG isla
nd
(+30
to +
380)
CpG isla
nd
(+61
0 to
+97
0)
Set 1 2 3
1.HANK12.NK923.YT4.SNK15.M.SssI treated lymphocyte DNA6.Bisulfite treated lymphocyte DNA 7.No template8.NKYS9.KHYGI10.SNK611.KAI312.M.SssI treated lymphocyte DNA13.Bisulfite treated lymphocyte DNA 14.No template
Methylation Specific PCR
400 300
300 200
400 300
1 2 3 4 5 6 7
8 9 10 11 12 13 14
1 2 3 4 5 6 7
8 9 10 11 12 13 14
Set 1
Set 2
Set 3
DN
A lad
der
Met
UnM
Met
UnM
Met
UnM
Met
UnM Met
UnM
Met
UnM
Met
UnM
1 2 3 4 5 6 7
8 9 10 11 12 13 14
Methylation Specific PCR
DN
A la
dder
SINE
1 2 3 4 5 6
8 9 10 11 12 13
300 200
LTR400
3001 2 3 4 5 6 7
8 9 10 11 12 13 14
Met
UnM
Met
UnM
Met
UnM
Met
UnM
Met
UnM
Met
UnM
Met
UnM
1 2 3 4 5 6 7
8 9 10 11 12 13 14