Slide 1

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(HPLC-DAD) DIONEX. : Ultimate 3000 LPG , : Ultimate 3000 Autosampler WPS-3000SL (Analytical), Ultimate TCC-3000-SD C18 3m, 250x3.0mm : Photometer Ultimate DAD-3000.

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CH3OH:H2O 1:1 (DMSO) (DMF). 200l HCl 37% 200l CH3OH:H2O 1:1. 100C 10 . HCl 50-60C. DMSO 2 .

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: ) H2O 0,1% TFA ) CH3CN 0,1% TFA . 38 . : 35C. 0.5ml/min. 50l. Chromeleon.

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Retention (min)Flow (ml/min)%%0.00.59550.00.59551.00.595515.00.5703020.00.5406023.00.5406033.00.559535.00.559538.00.5955

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) 275nm ) .)) 4:

(min) (nm) 13.2202, 223, 275, 310, 495 14.1201, 253, 367 ( )15.5201, 225, 286, 49317.1262, 440, 4653,3,4,7-18.0209, 247, 321, 3592--1,4-18.4206, 249, 278, 33819.0201, 253, 35319.2204, 236, 288, 54220.1204, 255, 37221.2202, 229, 265, 514, 54220.8263, 326, 433, 45721.3209, 268, 33521.4208, 260, 33021.5200, 219, 266, 36521.9207, 292, 54923.2203, 249, 277, 428--23.9200, 248, 41424.1201, 266, 366-24.2200, 249, 42024.3202, 243, 285, 335, 60624.4201, 263, 42324.5201, 243, 280, 41024.5204, 256, 295, 48026.6200, 223, 267, 288, 43927.0204, 246, 279, 410 4:

4:

dcII dcIVdcVII 13.0913.315.916.721.05285, 436276, 308, 493277, 311, 491276, 311, 491197, 218, 285, 426 4:

20.921.05274, 308, 489197, 218, 285, 426dcII dcIVdcVII 13.0913.315.916.721.05285, 436276, 308, 493277, 311, 491276, 311, 491197, 218, 285, 426 2515.5224, 308, 48923.224.426.6249, 277, 428256, 292, 479246, 279, 410 Type BType C11.8214.5616.37209, 255, 288204, 259, 372, 448204, 258, 287Persian Berries20.121.522.824.326.6255, 372266, 365256, 368254, 372287, 440 4:

20.121.421.6249, 277, 428256, 292, 479246, 279, 41017.719.4248, 321, 359257, 269, 39320.121.3253, 266, 346260, 325---24.524.223.9263, 424251, 420249, 41417.120.8262, 440, 465260, 326, 431, 45724.3285, 335, 606 . (HPLC) . . , . . , . , . . . , . , MSc ,., . Dimitrios Mantzouris, Ioannis Karapanagiotis, Lemonia Valianou, Costas Panagiotou, HPLC-DA-MS analysis of dyes identified in textiles from Athos, Anal Bioanal Chem (2011) 399: 3065-3079Dominique Cardon, Natural Dyes-Sources, Tradition, Technology and Science, Archetype Publications, London 2007, ISBN: 978-1-904982-00-5.I Degano, E. Ribechini, F. Modugno, and M. P. Colombini, Analytical Methods for the Characterization of Organic Dyes in Artworks and in Historical Textiles, Applied Spectroscopy Review 44 (2009) 363-410.Judith H. Hofenk de Graaff , The colourful past-Origins, chemistry and identification of natural dyestuffs, Archetype Publication, Switzerland 2004, ISBN 3-905014-25-4.Anna Karatzani and Thilo Rehren, The Use of Metal Threads and Decorations in ByzantineGreek Orthodox Ecclesiastical Textiles, Research Summary Archaeotechnology (2006) 34-37.Robin Gale R. Owen-Crocker, Medieval clothing and textiles, .3, 2007, ISBN 97818438322911. Satinder Ahuja, Chromatography and separation scince, v.4, Elsevier Science, USA, 2003.L.R. Snyder, J.J. Kirkland, Introduction to Modern Liquid Chromatography, second ed., John Wiley & sons, inc, USA 1979, ISBN: 0-471-03822-9.