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Basic Chemistry and Microbiology
Review of Basic Chemistry
smallest basic particle is the atom› Electrons- negatively charged› Protons- positively charged in nucleus› Neutrons- uncharged in nucleus
when electrons are lost or gained, a charge occurs
substances containing only one kind of atom are called elements
approximately 20 elements are found in all living things
carbon, hydrogen, oxygen, and nitrogen make up 97%
the other 16 are called trace elements
Molecules are when two or more atoms are joined together (Ex: O2, CO2 )
Compounds and Solutions
Two or more atoms or molecules joined in a definite proportion by weight is called a compound
Compounds have different characteristics from elements they are made from
Represented by a formula
Compounds and Solutions
Two or more atoms or molecules joined in a definite proportion by weight is called a compound
Compounds have different characteristics from elements they are made from
Represented by a formula
Compounds and Solutions
Types of Compounds› Inorganic
Do not contain carbon Often has a metal as a positive ion
› Organic Found in living things Always contain carbon When with hydrogen they are called
hydrocarbons (usually gases) When with other carbons, they bond in
chains
Compounds and Solutions
Solutions› Chemical process take place in solutions› A solution is when one substance dissolves
into another› The solute is dissolved into the solvent› If it dissolves it is soluble, if not, insoluble
› Note: in microbiology, a tincture is alcohol and some other substance
Carbohydrates
All have carbon (C), hydrogen (H), and oxygen (O)
Occur in ration of 1:2:1
Carbohydrates
Monosaccharides› Single or simple sugars› Glucose, fructose, galactose› They are isomers (same formula, but
different arrangement)
Carbohydrates
Glucose (also known as dextrose)› Carried in bloodstream› Combines with oxygen (oxidation) and
produces adenosine triphosphate (ATP) Fructose (found in fruits and honey)
› Sweetest of all monosaccharides Galactose
› Found in small amounts in agar, flaxseed, and milk
NOTE: “ose” means sugar
Carbohydrates
Disaccharides › Known as a double sugar› Examples: sucrose(table sugar), lactose
(milk sugar)and maltose (malt sugar) Chemical reaction to join:
› Dehydration synthesis (opposite to break apart is hydrolysis)
Dehydration Synthesis
+ = Disaccharide
Hydrolysis
Disaccharide + =
Carbohydrates
Oligosaccharides› Form chains called polymers› Small chains with only 2-10 monosaccharides› Ex: insulin
Polysaccharides› Large, complex molecules› Made of hundreds of thousands of glucose› Have very long polymer chains› Ex: starch, cellulose, and glycogen
Carbohydrates
https://www.youtube.com/watch?v=M6ZLDJluj6I
http://www.khanacademy.org/partner-content/crash-course1/crash-course-biology/v/crash-course-biology-103
Lipids
Examples are fats, oils, and waxes Like carbs, they have carbon, hydrogen,
and oxygen, but much less oxygen Fats are solid and oils are liquid at room
temperature Better sources of energy than
carbohydrates (yield more energy) but are harder to oxidize
Three groups: simple lipids, compound lipids, and derived lipids
Lipids
Simple Lipids (triacylycerol or triglyceride) contain one glycerol molecule and
three fatty acids held together by ester linkages
Formed by dehydration synthesis
Lipids
Simple Lipids Saturated (when all carbon bonds are
single and saturated with hydrogen)› Can block arteries› Difficult to break up› Raises cholesterol› Solid at room temperature
Lipids
Simple Lipids Unsaturated (when two or more hydrogen
bonds are replaced with double bonds between carbon atoms)› Liquid at room temperature› Monounsaturated (lacks 2 hydrogen bonds)
or Polyunsaturated (lacks 8 or more hydrogen bonds forming 4 or more double bonds)
› Lowers Cholesterol› Easier to break up
Lipids
Trans Fats› Unsaturated fat but act like a saturated fat› Has trans arrangement in bonding- the
hydrogens are on opposite sides of the double bond.
› Typical man-made
Lipids
https://www.youtube.com/watch?v=3xF_LK9pnL0
https://www.youtube.com/watch?v=VGHD9e3yRIU
Lipids
Compund lipids- lipids which contain an inorganic or organic group in addition to fatty acids and glycerol. › Phosphpholipids- Lipids containing a
phosphate group. A phospholipid molecule has a strongly nonpolar and hydrophobic (water insoluble) tail region represented by fatty acid chains and a strongly polar or hydrophilic (water soluble) head region
represented by the phosphate group.
Lipids
› Glycolipids- These are lipids containing a carbohydrate group, usually galactose. They are found in the nerve cell membranes especially in the myelin sheath.
Lipids
› Lipoproteins- These are lipids, usually phospho-lipids which contain a protein molecule. They occur in the cell membrane. They are also found in milk and egg yolk
Lipids Derived Lipids (Sterols)
› Contain only carbon, hydrogen, and oxygen› Include steroids found in male and female
hormones, Vitamin D, cholesterol, and fat soluble vitamins A, E, and K
› Classified as lipids only because they are soluble in fat solvents
› These are lipids that do not have a straight chain. They are composed of four fused carbon rings and a long hydrocarbon side chain.
Lipids
Derived Lipids
Proteins
Contain hydrogen, oxygen, and nitrogen and most times phosphorus and sulfur
Found in every part of living cells Coat viruses In binding and structural components
like fingernails, hair, ligaments, muscles, etc.
Made of AMINO ACIDS
Proteins
Structure of Amino AcidPosition 1 is carbon, Position 2 is amino group (NH2), Position 3 is hydrogen atom, Position 4 is a carboxyl group (COOH), and Position 5 is variable (R)
Proteins
Ex: Glycine (H is in R group)
Large protein molecules are constructed from any number and sequence of amino acids (can number from 300 to thousands)
Proteins
Amino acids linked by dehydration synthesis (C-N) (bond between carboxyl group of one amino acid and amino group of next amino acid). Called a PEPTIDE BOND and a series of linkages is called a POLYPEPTIDE
THIS IS CALLED A PROTEIN
Proteins
Structure› Primary- straight chain› Secondary- helix› Tertiary- twisted and folded› Quaternary- two or more polypeptide
chains are bonded together
Proteins
http://www.youtube.com/watch?v=lijQ3a8yUYQ
https://www.youtube.com/watch?v=2Jgb_DpaQhM
Proteins
Functions:
Enzymes - proteins that allow chemical reactions to occur in living things Antibodies – proteins that protect the body from
infection Structure – cytoskeleton, hair, nails, muscles, spider
web, silk, feathers ,horns, hooves etc…. Hormones – chemical messengers Cell membrane – proteins can act as channels through
the cell membrane - receptor proteins found on membrane transmit signals to the inside of cells
Hemoglobin – protein found in blood that carries oxygen
Proteins
Proteins
Denatured proteins are proteins that lose their shape - if they lose their shape, they also lose their - What can cause a protein to become denatured? Exposure to: Strong Acid Strong Base Heat Organic solvent: Alcohol or
Acetone - Denatured proteins can lose quaternary, tertiary
and secondary structure - Primary Structure is left untouched
Proteins (enzymes)
Specialized proteins Help provide energy to cell at just the
right moment and at just the right speed
Also known as organic catalysts Highly specific Very large and complex
Proteins (enzymes)
Made of either all protein or part protein (apoenzyme)attached to a non-protein part (coenzyme)
Coenzymes could be calcium, iron, magnesium, copper, or vitamins like C and B-complex
Proteins (enzymes)
The localized site on the enzyme molecule is called the active site
Each enzyme has its own pattern on the active site (no 2 alike)
An enzyme reacts with a reactant whose molecular pattern fits the enzyme’s molecular pattern.
The molecule that the enzyme reacts with is called a SUBSTRATE molecule
Proteins (enzymes)
Lock and key model
Proteins (enzymes)
Temporary physical binding called
Enzyme-Substrate Complex
Proteins (enzymes)
Enzymes are proteins and if they are exposed to extremes of temp or pH lose their shape - if a protein loses its shape, it loses its
function - a protein that loses its shape is said to
be denatured - if an enzyme is denatured, substrate
cannot enter the active site
Proteins (enzymes)
extreme temperatures or pH
Proteins (enzymes)
Name usually ends in –ASE Added to stem word taken from
substrate Examples:
› Lactase……lactose› Lipase……...lipids› Maltase……maltose› Protease…...protein› Sucrase…….sucrose
Proteins (enzymes)
An example in microbiology:
Many bacteria have an enzyme that needs a compound called PABA (para-aminobenzoic acid). PABA helps bacteria make a vitamin called folic acid, which the bacteria need to grow.
Proteins (enzymes)
An example in microbiology:
When antibiotic sulfanilamide is given to the bacteria instead of PABA, the sulfanilamide molecules attach to active sites of bacteria and folic acid is not made.
Proteins (enzymes)
Nucleic Acids
Contain carbon, oxygen, hydrogen, nitrogen, and phosphorous
Two types- DNA and RNA
Nucleic Acids
DNA› Found in chromosomes and genes, plasma
membrane, mitochondria, and chloroplasts
› Consists of a phosphate group, deoxyribose sugar and one of four nitrogenous bases: adenine, thymine, cytosine, and guanine
Nucleic Acids
Nucleic Acids
RNA› Consists of a phosphate group (ribose
sugar), and any of the following nitrogenous bases: adenine, cytosine, guanine, and uracil
› Single stranded› Found in cytoplasm, nucleoli, and
ribosomes› Two kinds: mRNA and tRNA