02

Microscope

A B

Carrying a Microscope

• Return the lowest power objective in place

• Wrap the cord around the base

• Return dustcover

Storing The Microscope

 

• Use lens paper on all glass parts of the microscope.

• Clean oil immersion lens with chemicals provided by your instructor

Cleaning the Microscope

Parts of the Microscope

• Fine adjustment knob

• Stage

• Stage clips

• Aperture

• Diaphragm

• Light

• Base

• Ocular

• Body tube

• Nosepiece

• Objective lenses (10, 40, 100x)

• Arm

• Course adjustment knob

Microscope Parts

A. OcularB. Body tubeC. HeadD. Revolving nose pieceE. ObjectiveF. ArmG. StageH. DiaphragmI. Lever to move stage clipJ. Course adjustmentK. Fine adjustmentL. Light sourceM. Base

Using the microscope

• Always observe using the LOWEST POWER objective first.

• Focus using the COARSE ADJUSTMENT KNOB to bring the object into focus. Bring the object into sharp focus by using the fine adjustment knob.

• Focus, and then move to a higher power objective, if needed.

• Use only the FINE ADJUSTMENT KNOB when using the HIGHEST (longest) POWER OBJECTIVE.

• Keep both eyes open to reduce eyestrain. • Determine total magnification of the object by

multiplying the power of the ocular (10x) the power by the power of the objective.

Preparing a slide

• Using a pipet or dropper, add a drop of water or another solvent to a clean microscope slide. Then, place the specimen in the water.

• Place the edge of a coverslip on the slide so that it touches the edge of the water.

• Slowly lower the coverslip to prevent the formation of air bubbles.

40x 100x

Oil Immersion Lens

Animal Cell

Plant Cell

Cheek Cell

Dispose Biological material

Dispose Sharps

Clean Up

Microscope Parts Quiz

Microscope Quiz

1. Which objective uses oil?2. If your ocular is 10x and your

objective is 40x what is the total magnification of your image?

3. Why do you need a cover slip and how do you avoid an air bubble?

4. Where do you dispose slides?

P. 45 lab manual

sperm

blood

smooth muscle

Simple squamous epithelial

Cheek Cell

0.9% saline

Cheek Cell

saline

1. Scrape inside cheek2. Swirl toothpick with cells in saline3. Place a small drop methylene blue on saline

Cheek Cell

1. Place coverslip2. Observe under microscope3. Draw what you see4. Clean up

Cheek Cell