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POROS® Affinity ColumnsProtein A, Protein G, and CaptureSelect® Affinity Columns

Intended for analytical scientists supporting the development of biologic drugs.

Or for small-scale purification of immunoglobulins, Fabs, or fusion proteins.

POROS® Affinity Columns enable:Direct sampling from cell culture or fermentation harvests

Precise quantitation of product titers, typically in under 3 minutes

with the eluted product being highly purified and suitable for further analysis including

mass spectrometry.

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POROS® CaptureSelect® ColumnsA collaboration between Life Technologies and BAC

POROS® Affinity ColumnsPrepacked column overview

Column Dimension

Diameter (mm) x Length (mm)

Column Volume

(mL)

2.1 x 30 0.1

4.6 x 50 0.8

4.6 x 100 1.7

10 x 100 7.9

Product Name Specificity Dynamic Binding Capacity (mg/mL)

POROS® A 20 Human, mouse, and 5 other

species

19.0

POROS® G 20 Human, mouse, rat, and 8 other

species

9.0

POROS® CaptureSelect® IgG Fc Human 8.9

POROS® CaptureSelect® IgM Human, mouse, and rat

4

POROS® CaptureSelect® LC Kappa Human, all subclasses

14.5

POROS® CaptureSelect® LC Lambda Human, all subclasses

10

POROS® CaptureSelect® HSA Human 2.6

POROS® prepacked columns

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POROS® Affinity ColumnsProtein A, Protein G, and CaptureSelect® specificity

CaptureSelect®Kappa or Lambda

CaptureSelect®IgG Fc

CH3

CH1

CH2

VH

CL

VL

Protein A or Protein G

POROS® Protein A, Protein G, and CaptureSelect® ligands target multiple IgG epitopes. The graphic shows the binding sites for affinity ligands Protein A and Protein G. Novel POROS® CaptureSelect® ligands provide alternative specificities that enable rapid binding for the quantitation or purification of engineered immunoglobulins.

POROS® Protein A & Protein G resins

POROS® CaptureSelect® ligands

Bimolecular type

Protein A

Protein G

IgG Fc Kappa Lambda HSA IgM

IgG1, IgG2, IgG4

IgG3 IgG, CH2

domain deleted

IgM IgA, IgD, IgE

Fab, Fab'2 Human serum albumin

Albumin fusion protein

IgG Fc fusion protein

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POROS® Affinity ColumnsAdditional information

Product Bulletin: POROS® Protein A, Protein G, and CaptureSelect® Columns

Operating Instruction: POROS® Protein A, Protein G and CaptureSelect® Affinity Columns

Application Note: POROS® A 20 Analytical HPLC Columns

Go to BAC: bacbv.com

© 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their

respective owners. CaptureSelect is a registered trademark of BAC BV.