100 bp

200 bpSMb21314 (wgeA)

SMb20946 (exoY)

SMb20094

SMa1077 (nex18)

SMa4202 (rhbB)

M10

0bp

AtG

MI9

023

1 2 3 4 5 6 7 8 M10

0bp

1021

Rct

AH

B

Transconjugants (pSymA + pSymB)

Figure S2. PCR amplication of genes from pSymA and pSymB in A. tumefaciens GMI9023 transconjugants that received both plasmids simultaneously. The recipient strain AtGMI9023 was PCR-negative for all marker genes, and the donor strain 1021RctAHB was used as a positive control. Note that transconjugants 1 and 2 only amplified SMa1077 and SMb20094 genes, whereas the remaining transconjugants examined gave positive amplification of all 5 genes tested. M100bp, DNA molecular weight marker.