13TH INTERNATIONAL SYMPOSIUM ON BIOLUMINESCENCE & CHEMILUMINESCENCE

DATE

August 2 (Mon.) to 6 (Fri.), 2004

VENUE

Pacifico Yokohama 1-1-1 Minato Mirai, Nishi-ku, Yokohama 220-0012, Japan

TEL +81-45-221-2155 URL: http://www.pacifico.co.jp/index_e.html

version 7/26/04

SCHEDULE OF ISBC2004Aug 2 (Mon)

3rd floor Lounge ROOM 301 ROOM 303 ROOM 304 ROOM 302AM 09:00 AM 09:00-09:15

Welcome: Tsuji A

10:00

11:00

12:00

PM 01:00

02:00

03:00

04:00 Break

05:00

06:00 PM 06:15-07:00ISBC general meeting

Lunch break

AM 10:15-11:00Plenary lecture #1(Chair: Inouye S)

Shimomura O

AM 09:15-10:00Memorial lecture for Late Prof.Goto T(Chair: Tsuji A)

Niwa H

AM 09:00-PM 06:00Poster session #1,Exhibition

Setup:Aug 2,PM 03:00-06:00Aug 3,AM 08:00-09:00

Begins:Aug 3, AM 09:00

Ends/ Take down:Aug 4, AM 10:00

Coffee break

TimeAug 3 (Tue)

AM 11:00-12:00Poster session #1, Discussion

PM 02:00-06:00Registration

PM 03:30-Coffee, Wine &Cheese

Coffee break

PM 02:30-04:00Session #1Mechanism and TheoreticalStudy of ChemiluminescentReactions

(Chairs: Adam W, YamaguchiK)1. Kimura M2. Fujimori K3. Tanaka C4. Yamaguchi K

PM 04:15-05:45Session #2Color of Chemiluminescenceand Bioluminescence

(Chairs: Akhavan-Tafti H, KatoH)1. Branchini B2. Matsumoto M3. Kato H4. Kimura J

PM 02:30-06:00Session #4Recent Development inEnvironmental Fields

(Chair: Hayakawa K)1. Lin J-M

(Chairs: Ripp S, Nakashima K)2. Kawano T3. Wiles S4. Zomer G5. Inazu K

(Chairs: Lin J-M, Kuroda N)6. Tu S-I7. Ripp SA8. Boyandin AN9. Kratasyuk VA

PM 01:30-02:15Plenary lecture #2(Chair: Matsumoto M)

Adam W

PM 02:30-05:45Session #3Luminescent Bio-imaging andBiosensors Developed withNanomolecules andNanoparticles

(Chairs: Tamiya E, Ohtani T,Tsujimoto K)1. Tamiya E2. Ohtani T3. Sakaguchi T4. Shah D5. Bakalova R6. Zhang ZF7. Kimura J8. Paiziev A

1

SCHEDULE OF ISBC2004

AM 09:00

10:00

11:00

12:00

PM 01:00

02:00

03:00

04:00

05:00

06:00

TimeROOM 301 ROOM 303 ROOM 304 ROOM 302

Break

PM 01:30Excursion to Hakkeijima Sea Paradise or Historical Town Kamakura or Yokosuka City Museum

AM 10:00-11:10Session #7Synthesis of CompoundsRelating toChemiluminescence

(Chairs: Brancini B, Fujimori K)1. Akhavan-Tafti H2. Motoyoshiya J3. Hirano H

AM 11:25-12:40Biolumin. Chemilumin. FestivalPlaza (BCFP)

(Chairs: Adam W, Kimura M,Fujimori K)

Lunch break

AM 09:00-12:00Poster session,Exhibition

AM 10:00Poster session #1Ends/ Take down

AM 10:30Poster session #2Setup

AM 09:00-09:45Plenary lecture #3(Chair: Masujima T)

Nagano T

Coffee break

AM 10:00-12:00Session #5Biological Application withFluorescent Biomolecules andCells

(Chairs: Yokoyama K,Tsujimoto K)1. Nagai T2. Miwa Y3. Yokoyama K4. Kaihara A5. Hoffman RM6. Hickey PC

AM 10:00-12:20Session #6Bioluminescence; Evolutional,Biological and EcologicalAspects

(Chairs: Viviani V, Meyer-Rochow VB)1. Wada M2. Mallefet J3. Rivers TJ4. Meyer-Rochow VB5. Suzuki H6. Branham M

Aug 4 (Wed)

2

SCHEDULE OF ISBC2004

AM 09:00

10:00

11:00

12:00

PM 01:00

02:00

03:00

04:00

05:00

06:00

TimeROOM 301 ROOM 303 ROOM 304 ROOM 302 ROOM 301 ROOM 302

Break

AM 09:00-12:00Educational Session

Scientific EducationalSymposium for Early Teens

"Firefly Sheds Light on NewTechnology"

Closing

Lunch break

PM 03:15-06:00Session #12Development in BiomedicalFields II

(Chairs: Roda A, Totani M)1. Kikuchi K2. De Sole P3. Fan F4. Hanai T5. Tsukagoshi K

(Chairs: Kai M, Nozaki O)6. Tsunoda M7. Nakazono M8. Yamada M9. Gehring AG10. Zhao L11. Frank LA

PM 03:15-04:55Session #13Functional Chemi-Luminophore

(Chairs: Suzuki N, Kimura M)1. Okamoto H2. Yoshinaga T3. Cui H4. Koto T5. Niina N

Coffee break

PM 03:15-05:55Session #11Basic and Applied Studies ofMarine and BacterialBioluminescence

(Chairs: Lee J, Karatani H)1. Karatani H2. Kronstrom J3. Haddock H4. Markova SV5. Kuse M6. Vanderlinden C7. Lee J8. Vysotski ES9. Mastroianni N

PM 01:30-02:15Poster session #2, Discussion

PM 02:15-03:00Plenary lecture #5(Chair: Nakashima K)

Umezawa Y

AM 10:00-12:10Session #10Frontiers of IndustrialApplication of Firefly Luciferase

(Chairs: Lundin A, MatsuyamaA)1. Lundin A2. Venkateswaran K3. Daumer K4. Ramsay CM5. Tisi L6. Kamahori M

PM06:30-08:30Banquet & Award of DeLuca Prizes3F "Pacific" room, InterContinental Hotels YOKAHAMA GRAND IC

AM 09:00-PM 06:00Poster session #2,Exhibition

Setup:Aug 4,AM 10:30-12:00Aug 5,AM 08:00-09:00

Begins:Aug 5, AM 09:00

Ends/ Take down:Aug 6, AM 11:00

AM 09:00-09:45Plenary lecture #4(Chair: Ohashi M)

Tsuji FI

Coffee break

AM 10:00-12:05Session #9Development in BiomedicalFields I

(Chairs: Kricka L, Santa T)1. Roda A2. Kai M3. Yoshiki Y4. Szalay AA5. Nozaki O

PM 04:55-06:15Session #14Singlet oxygen and Superoxide

(Chairs: Hirano T, Trofimov AV)1. Suzuki N2. Bancirova M3. Kazakov DV4. Trofimov AV

AM 10:00-12:00Session #8Basic and Applied Studies ofBeetle Bioluminescence

(Chairs: Watanabe H, OhmiyaY)1. Viviani V2. Ohba Y3. Ugarova NN4. Ayabe K5. Brovko LY6. Nakajima Y

Aug 5 (Thu) Aug 6 (Fri)

AM 09:00-12:00Poster session,Exhibition

AM 11:00Poster session #2Ends/ Take down

3

WELCOME AND INTRODUCTION Welcome! On behalf of the Organizing Committee of 13th International Symposium on Bioluminescence & Chemiluminescence 2004 in Yokohama (13th ISBC), I would like to welcome you to the Symposium. The first symposium was held in 1978 in Brussels, Belgium and the symposium has subsequently been held every two years in Europe and America. This symposium is the first to be held in Japan, as well as in the Asia region.

At the first day, the symposium begins with the Memorial Lecture of Late Professor Toshio Goto of Nagoya University presented by Professor H. Niwa, The University of Electro-Communications, and following the Plenary Lectures of Professor O. Shimomura, Marine Biology Laboratory and Professor W. Adam, the University of Puerto Rico. The plenary lectures will presented by Professor T. Nagano, the University of Tokyo, Professor of F. Tsuji, the University of California, and Professor Y. Umezawa, the University of Tokyo at the second to third day, respectively.

These plenary lectures will be followed by symposium sessions on Mechanism and Theoretical Study of Chemiluminescent Reactions, Color of Chemi- and Bioluminescence, Luminescent Bio-imaging and Biosensors developed with Nanomolecules and Nanoparticles, Recent Development in Environmental Fields, Biological Application with Fluorescent Biomolecules and Cells, Bioluminescence; Evolutional, Biological and Ecological Aspects, Synthesis of Compounds related to Chemiluminescence, Basic and Applied Studies of Beatle Bioluminescence, Development in Biological Fields, Frontiers of Industrial Application of Firefly Luciferase, Basic and Applied Studies of Marine and Bacterial Bioluminescence, Functional Chemi-Luminophore and Singlet Oxygen and Superoxide Bioluminescence Chemiluminescence Festival Plaza will be planed for free talk and discussion. Moreover, the education session “ Firefly Sheds Light on New Technology” will be opened to the early teens as scientific education.

Poster presentations which cover and wide array of topics and expand on information presented during the oral sessions are the Exhibit Hall. In addition, the Exhibit Hall is full of the latest technology to make our studies. We hope you will take time to visit each of our exhibitors during the symposium.

Bus tour to Hakkeijima Sea Paradise (Aquarium) or Walking tour around historical town Kamakura or Scientific visit to Yokosuka City Museum is prepared to all participants.

All attending owe much to the diligent Program Committee and to the Session Chairs who powerful assisted in attracting keynote speakers and in giving logical organization to the submitted papers. Our generous sponsors, listed elsewhere, improved the quality of the amenities of the meeting.

On behalf of the entire organizing committee, welcome. We are glad you are here. We hope you will enjoy the next few days and that you have a valuable and rewarding learning experience as well.

Akio Tsuji, PhD The President of 13th International Symposium on Bioluminescence and Chemiluminescence

ORGANIZATION PRESIDENT

Akio Tsuji (Emeritus Professor, Showa University) VICE PRESIDENT

Masakatsu Matsumoto (Professor, Kanagawa University) Masuo Aizawa (President, Tokyo Institute of Technology)

INTERNATIONAL ADVISORY BOARD

ISBC Council Members: J. Lee (President) A. Roda (Past President) A. Szalay (President Elect) P. Pasini (Secretary) P. Stanley (Past Secretary) B. Branchini (Treasury & Membership) L. Kricka (Publication Officer)

Councilor A. Berthold S. Haddock P. Hill V. Karatasyuk M. Maeda J-F. Rees E. Widder B. Zomer

Scientific Advisory Board S. Daunert P. DeSole P. Schaap D. Shah A. Tsuji N. Ugarova V. Viviani J.M. Lin SD. Tu

NATIONAL ADVISORY BOARD

M. Ohashi J. Goto T. Nagano Y. Umezawa T. Masujima H. Utsumi

SCIENTIFIC PROGRAM AND LOCAL ORGANIZING COMMITTEES

N. Amino Y. Ashihara H. Arakawa M. Fukuoka K. Fujimori K. Hayakawa S. Harada M. Hiramatsu S. Hosaka K. Imai M. Isobe M. Inoue S. Inouye I. Imada M. Kimura Y. Kasahara N. Kuroda N. Hattori K. Nakashima H. Niwa O. Nozaki K. Ohmiya N. Suzuki K. Tsujimoto T. Santa M. Totani T. Toyo’oka N. Wada M. Yamaguchi

GENERAL SECRETARIAT

Masako Maeda (Professor) School of Pharmaceutical Sciences, Showa University 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, JAPAN TEL: +81-3-3784-8193 FAX: +81-3-3784-8247 E-mail: maedam@pharm.showa-u.ac.jp

Official Symposium Web Site http://www10.showa-u.ac.jp/~ISBC/index.html (produced and designed by K. Ito, Showa University)

E-mail Address isbc2004@pharm.showa-u.ac.jp

ACKNOWLEDGEMENT We wish to express our sincere appreciation to the following for their generous support of this symposium. Commemorative Organization for Japan World Expositive ’70 Japan National Tourist Organization The Foundation of Pharmaceutical Manufacturers’ Association of Japan The Nagai Foundation The Tokyo Biochemical Research Foundation Mochida Memorial Foundation for Medical and Pharmaceutical Research The Naito Foundation The Research Foundation for Pharmaceutical Sciences Tokyo Ohka Foundation for the Promotion of Science and Technology Sankyo Life Science Foundation Suntory Institute for Bioorganic Research Uehara Memorial Life Science Foundation Abbott Japan Co. Ltd. Aventis Pharma Ltd. Berthold Japan Co. Chemco Scientific Co. Ltd. Chisso Corporation Fujirebio Inc. Hamamatsu Photonics K.K. Hitachi Ltd. Horiba Biotechnology Co. Ltd. IBL Co., Ltd. JASCO Corporation Kikkoman Corporation Kowa Company Ltd.

Lumica Corporation Mitsubishi Pharma Corporation Nissin Scientific Corporation Novartis Pharma K.K. Otsuka Pharmaceutical Co. Ltd. Roche Diagnostics K.K. Sankio Chemical Co., Ltd. Thermo Electron K.K. Tokken Inc. Tokyo Rikakikai Co. Ltd Tosoh Corporation Tohoku Electric Industrial Co. Ltd. Wako Pure Chemical Industry Ltd. (in alphabetical order)

REGISTRATION The registration desk will be open at 3rd floor of Pacifico Yokohama.

• Aug 2 (Mon), PM 02:00-PM 06:00 • Aug 3 (Tue), AM 08:00-PM 06:00 • Aug 4 (Wed), AM 08:00-AM 12:00 • Aug 5 (Thu), AM 08:00-PM 06:00 • Aug 6 (Fri), AM 09:00-AM 12:00

All participants must register and wear their nametags throughout the symposium. Symposium materials (final program, abstract book, nametag, etc.) will be provided to registered participants. All general information services, including lost-and-found articles and travel information, also can be obtained at this desk.

REGISTRATION FEES

ISBC JABC Member Non-Member Students Accompanying

Person Early Registration By May 31, 2004 35,000 yen 45,000 yen 20,000 yen 15,000 yen

Regular / On-Site Registration

After June 1, 2004 40,000 yen 50,000 yen 25,000 yen 20,000 yen

Entitlements Participant / Student Registration Fee includes:

• Admission to all scientific sessions • Final programme and abstract book • Welcome reception • Meal coupons, Coffee breaks • Excursion • Banquet • Symposium Proceeding Volume (available approx. the 2nd quarter of 2005)

Accompanying Person's Registration Fee includes: • Welcome reception • Meal coupons • Excursion • Banquet

ISBC MEETINGS

Date Time Location Purpose

Aug 3 PM 12:00-01:00 ROOM 315 Meeting of “Old” ISBC Council ISBC council members only.

Aug 3 PM 06:15-07:00 ROOM 301 General Meeting of the Society All ISBC members are welcome.

Aug 5 PM 12:15-01:15 ROOM 315 Meeting of “New” ISBC Council ISBC council members only.

Aug 5 PM 06:15- ROOM 315 Meeting of the Committee for the Marlene DeLuca Prize

INFORMATION FOR PRESENTERS ORAL PRESENTATION Presentation

Please stand by 10 minutes prior to your presentation at the designated seat. Presentation Time

Each speaker has been allocated different time for oral presentation. Please check your presentation time and start time from final programme. The presentation time is including several minutes for discussion.

Equipment

LCD projector is the only available equipment at the presentation room. Basically, you should bring your own computer (PC or Mac) with 15-hole (mini D-sub 15pin) connector and also your presentation data on a CD-ROM (ISO9660) or USB memory storage device. Floppy disks, Zip disks and MO disks cannot be used. If you have movie or sound data in your presentation, please contact us in advance. We will provide PCs (not Mac) against emergencies. Please prepare your presentation data by Microsoft PowerPoint and standard OS fonts. We recommend that you bring a backup copy of your slides in transparency (OHP) sheet.

Registration of your PC and CD-ROM At least 1 hour prior to your presentation, we would like you to check and register your presentation data at the presentation registration desk. Our operators will assist your data check and registrations.

POSTER PRESENTATION Poster Size

Size of poster board: 90 cm (Width) x 210 cm (Height) Please prepare a poster of the above maximum size to fit within the poster board. Include at the top of your poster the title, name(s) of author(s) and affiliation(s).

Schedule

SETUP BEGINS DISCUSSIONS* ENDS/ TAKE DOWN

POSTER SESSION #1 Aug 2, PM 03:00-06:00Aug 3, AM 08:00-09:00 Aug 3, AM 09:00 Aug 3, AM 11:00-12:00 Aug 4, AM 10:00

POSTER SESSION #2 Aug 4, AM 10:30-12:00Aug 5, AM 08:00-09:00 Aug 5, AM 09:00 Aug 5, PM 01:30-02:15 Aug 6, AM 11:00

*The discussion will take place at the poster exhibition room. Authors are requested to stand next to their poster during the discussion time in order to discuss their work with other participants.

Note

• Your presentation number indicates the location of your poster board. • Pushpins and Scotch tape will be provided at the presentation registration desk. • The transport of posters to the symposium is the responsibility of the authors. • No audiovisual equipment will be provided.

SCIENTIFIC PROGRAMME DAY 1 (Mon, Aug 2) PM 02:00-06:00 3rd floor Lounge Registration PM 03:30-PM 06:00 3rd floor Lounge Welcome Reception Wine, beer and other beverages will be served with cheese and light meals. Please enjoy after registration of the symposium. DAY 2 (Tue, Aug 3) AM 09:00-09:15 ROOM 301 Welcome Tsuji A, President of ISBC2004 AM 09:15-10:00 ROOM 301 Memorial Lecture for Late Prof. GOTO T (Chair: Tsuji A) New aspects on the molecular mechanism of Latia bioluminescence Niwa H AM 10:15-11:00 ROOM 301 Plenary Lecture #1 (Chair: Inouye S) Aequorin and GFP: an historical account Shimomura O AM 11:00-12:00 ROOM 302 Poster Session #1, Discussion PM 01:30-02:15 ROOM 301 Plenary Lecture #2 (Chair: Matsumoto M) On the CIEEL mechanism of triggerable dioxetanes: Does the electron jump or is it charge transfer? Adam W, Trofimov AV PM 02:30-04:00 ROOM 301 Session #1 Mechanism and Theoretical Study of Chemiluminescent Reactions (Chairs: Adam W, Yamaguchi K) PM 02:30-02:55 (keynote lecture) Relationship between heat of reaction and chemiluminescence efficiency of chemiluminescent reactions Kimura M, Iga H, Araki H, Matsumoto M PM 02:55-03:20 (keynote lecture) Dioxetane chemiluminescence mechanisms Fujimori K, Wakasugi T, Okamoto T, Nakajima T, Maeda K, Morihashi K, Takahashi O, Watanabe N, Matsumoto M, Asano T, Ohga Y

PM 03:20-03:40 Intramolecular CIEEL mechanism on chemiluminescence of phenoxide substituted 1,2-dioxetanes Tanaka C, Tanaka J, Matsumoto M PM 03:40-04:00 Theoretical studies on charge transfer mechanism for decomposition of dioxetanes and dioxetanones Isobe H, Okumura M, Yamaguchi K PM 04:15-05:45 ROOM 301 Session #2 Color of Chemiluminescence and Bioluminescence (Chairs: Akhavan-Tafti H, Kato H) PM 04:15-04:40 (keynote lecture) Bioluminescence color determination in firefly luciferase Branchini BR PM 04:40-05:05 (keynote lecture) Change of color and decay-rate by microenvironments for base-induced chemiluminescence of dioxetanes bearing a biaryl moiety Matsumoto M PM 05:05-05:25 Structural Basis of firefly luciferase reaction Kato H (Abstract in this book) PM 05:25-05:45 Green luminescence emitted from adsorbed oxygen Aurora has appeared on the surface of materials? Kimura J PM 02:30-05:35 ROOM 303 Session #3 Luminescent Bio-imaging and Biosensors Developed with Nanomolecules and Nanoparticles (Chairs: Tamiya E, Ohtani T, Tsujimoto K) PM 02:30-03:10 Advanced optical biosensors based on nano/micro technology Tamiya E PM 03:10-03:40 Scanning near field optical / atomic force microscopy (SNOM/AFM) -novel imaging technique in nano-meter scale- and DNA-nanoFISH method Ohtani T, Kim JM, Yoshino T, Nakao H, Sasou M, Sugiyama S, Hirose T, Muramatsu H PM 04:00-04:30 Rapid and onsite BOD sensing by luminous cells-immobilized-chip Sakaguchi T, Morioka Y, Tamiya E PM 04:30-04:45 Chagas assay using recombinant antigens on a fully automated chemiluminescence immunoassay analyzer Shah D, Chang C, Cheng Y, Jiang L, Salbilla V, Haller A, Schochetman G

PM 04:45-05:00 Application of highly luminescent quantum dot bioconjugates in protein imaging: quantum dot-based immunoblot analysis Bakalova R, Zhelev Z, Ohba H, Ishikawa M, Baba Y PM 05:00-05:15 Enhancement by gold nanoparticles of luminol chemiluminescence Zhang ZF, Lai CZ, Shi MJ, Cui H PM 05:15-05:30 Luminescence techniques in reinvestigation into photocatalytic reactivity of TiO2 Kimura J PM 05:30-05:45 In vivo observation the super weak luminescence of single living cotton cell Paiziev A, Krakhmalev V PM 02:30-06:00 ROOM 304 Session #4 Recent Development in Environmental Fields (Chair: Hayakawa K) PM 02:30-03:10 (keynote lecture) Microchip electrophoresis with chemiluminescent detection and its possible application to environmental analytical chemistry Lin J-M (Chairs: Ripp S, Nakashima K) PM 03:10-03:30 Noncompetitive inhibition of the rare earth element-induced oxidative burst by zinc in tobacco cells: A chemiluminescent analysis Kawano T, Kadono T PM 03:30-03:50 Dynamics of colonisation and clearance of the murine pathogen Citrobacter rodentium Wiles S, Clare S, Harker J, Huett A, Dougan G, Frankel G PM 03:50-04:10 Development of a sensitive field test for detecting nerve gases in water Zomer G, Hamzink M, Strick T, Bloemen H PM 04:10-04:30 Chemiluminescence detection of 3-nitrobenzanthrone and 2-nitrotriphenylene in airborne particles with on-line reduction HPLC system Inazu K, Saito T, Vu DN, Aika K, Hisamatsu Y (Chairs: Lin J-M, Kuroda N) PM 04:40-05:00 Bioenergetic confirmation of viable pathogens in foods by ATP-bioluminescence Tu S-I PM 05:00-05:20 Bioluminescent bioreporter integrated circuit sensing of the chemical and biological spacecraft environment Ripp SA, Garland J, Sayler GS

PM 05:20-05:40 Bioluminescence variability of lux-marked strain Escherichia coli Z905/pPHL7 in aquatic microcosms with different salinity Boyandin AN, Kargatova TV, Ganusova EE, Lobova TI, Popova LYu PM 05:40-06:00 Enzyme-based biosensors based on bacterial bioluminescence for environmental monitoring Kratasyuk VA, Esimbekova EN, Vetrova EV PM 06:15-07:00 ROOM 301 ISBC General Meeting All ISBC members are welcome. DAY 3 (Wed, Aug 4) AM 09:00-09:45 ROOM 301 Plenary Lecture #3 (Chair: Masujima T) Development of fluorescence probes for biological applications, based on photoinduced electron transfer Nagano T AM 10:00-12:00 ROOM 301 Session #5 Biological Application with Fluorescent Biomolecules and Cells (Chairs: Yokoyama K, Tsujimoto K) AM 10:00-10:20 New methods for development of FRET-based biosensors with expanded dynamic range Nagai T, Miyawaki A AM 10:20-10:40 Novel method for visualization of protein-protein interactions in living mammalian cells using Red fluorescent protein, DsRed Miwa Y, Tanaka J, Shigesada K, Goto K AM 10:40-11:00 Fluorescence sensor peptide for protein phosphorylation Yokoyama K, Kazuya Itoda K AM 11:00-11:20 Flashing a protein-protein interaction in living cells via split Renilla luciferase complementation Kaihara A, Umezawa Y AM 11:20-11:40 In vivo imaging with fluorescent proteins: The new cell biology Hoffman RM AM 11:40-12:00 Imaging fluorescence and bioluminescence in living cells Hickey PC

AM 10:00-12:20 ROOM 303 Session #6 Bioluminescence; Evolutional, Biological and Ecological Aspects (Chairs: Viviani V, Meyer-Rochow VB) AM 10:00-10:20 Expulsion of symbiotic luminous bacteria from pony fish, Leiognathus nuchalis Wada M, Barbara GM, Mizuno N, Azuma N, Kogure K, Suzuki Y AM 10:20-10:40 Bioluminescence in Ophiuroids (Echinodermata) Mallefet J AM 10:40-11:00 The mating system and alternate mating strategies of a marine bioluminescent Caribbean ostracode Rivers TJ, Morin JG AM 11:00-11:30 (keynote lecture) Luminescent behaviour in the New Zealand glowworm, Arachnocampa luminosa (Insecta; Diptera; Mycetophilidae) Ohba N, Meyer-Rochow VB AM 11:30-11:50 An evolutionary history of the Japanese aquatic fireflies inferred from mitochondrial DNA sequences Suzuki H, Sato Y, Ohba N AM 11:50-12:20 (keynote lecture) Flashers, glowers and sniffers: Sexual selection and signal evolution in fireflies Branham M AM 10:00-11:10 ROOM 304 Session #7 Synthesis of Compounds Relating to Chemiluminescence (Chairs: Brancini B, Fujimori K) AM 10:00-10:25 (keynote lecture) Chemiluminescent substrates for phosphatases and peroxidases Akhavan-Tafti H AM 10:25-10:45 Chemiluminescence involving the phosphorus chemistry. Phospha-1, 2-dioxetanes as the most likely high-energy intermediates in autoxidation of phosphonate carbanions Motoyoshiya J, Aoyama H AM 10:45-11:10 Development of the chemistry of the imidazopyrazinone-bioluminescence system: From the bio- and chemiluminescence mechanism to a design of sensor molecules Hirano T, Nakai S, Sekiguchi T, Fujio S, Maki S, Niwa H

AM 11:25-12:40 ROOM 304 Bioluminescence Chemiluminescence Festival Plaza (BCFP) Luminescence Co-starring Exhibition & Exciting Free Debating on Light Emitter Forming Mechanisms (Chairs: Adam W, Kimura M, Fujimori K) Panelists: Adam W, Akhavan-Tafti H, Brancini B, Fujimori K, Kato H, Matsumoto M, Tanaka J, Yamaguchi K PM 01:30- EXCURSION All participants are free.

A: HAKKEIJIMA SEA PARADISE B: HISTORIC TOWN OF KAMAKURA C: SCIENTIFIC TOUR TO YOKOSUKA CITY MUSEUM

DAY 4 (Thu, Aug 5) AM 09:00-09:45 ROOM 301 Plenary Lecture #4 (Chair: Ohashi M) Bioluminescence reaction in the firefly squid, Watasenia scintillans Tsuji FI (Abstract in this book) AM 10:00-12:00 ROOM 301 Session #8 Basic and Applied Studies of Beetle Bioluminescence (Chairs: Watanabe H, Ohmiya Y) AM 10:00-10:30 (keynote lecture) Luciferase-like enzymes in non-luminescent beetles: a model of beetle protoluciferase? Viviani V AM 10:30-10:45 Firefly luciferase and Drosophila CG6178 gene product are fatty acyl-CoA synthetases Oba Y, Ojika M, Inouye S AM 10:45-11:05 Bioluminescent spectra of native and mutant firefly luciferase as a function of pH Ugarova NN, Maloshenok LG, Uporov IV AM 11:05-11:20 Importance of firefly luciferase C-terminal domain in binding of luciferyl-adenylate Ayabe K, Zako T, and Ueda H AM 11:20-11:35 Comparison of kinetic properties of firefly luciferase from Photinus pyralis and Luciola mingrelica Brovko LY, Gandelman OA, Kershengolz IB, Ugarova NN AM 11:35-12:00 (keynote lecture) A novel tricolor reporter assay system for simultaneous monitoring of multiple gene expressions Nakajima Y, Ohmiya Y

AM 10:00-12:05 ROOM 303 Session #9 Development in Biomedical Fields I (Chairs: Kricka L, Santa T) AM 10:00-10:25 Luminescent probes for sensitive and specific optical microscope imaging Roda A, Guardigli M, Pasini P, Mirasoli M, Michelini E AM 10:25-10:50 Chemiluminescent polymeric probe for the sensitive detection of DNA Kai M AM 10:50-11:15 Application of imaging detection using XYZ emission system to food analysis Yoshiki Y AM 11:15-11:40 Visualization of tumors and metastases in live animals with injected bacteria and vaccinia virus encoding light-emitting proteins Yu YA, Shabahang S, Timiryasova TM, Zhang Q, Beltz R, Gentschev I, Goebel W, Szalay AA AM 11:40-12:05 Repetitive assay for enhanced detection of immobilized horseradish peroxidase by imidazole chemiluminescence coupled to the technique of on-line regeneration of inactivated peroxidase Nozaki O, Munesue M, Kawamoto H AM 10:00-12:10 ROOM 304 Session #10 Frontiers of Industrial Application of Firefly Luciferase (Chairs: Lundin A, Matsuyama A) AM 10:00-10:20 Overview of new analytical tools for bioluminescent biomass estimation Lundin A, Elvang A AM 10:20-10:50 (keynote lecture) Applications of bioluminescence-based assay in monitoring microbial burden Venkateswaran K AM 10:50-11:10 Bacteriophage-based bioluminescent bioreporters for monitoring opportunistic pathogens in the spacecraft environment Daumer K, Ripp S, Sayler G, Garland J AM 11:10-11:30 The use of ATP bioluminescence for monitoring biocide or disinfectant treatment of water Ramsay CM, Wayman D, Davenport K, Michie I (Abstract in this book) AM 11:30-11:50 A bioluminescent assay for real-time nucleic acid amplification Gandelman O, Church V, Murray J, Tisi L

AM 11:50-12:10 DNA analysis method by luciferase-based bioluminescence detection and a miniaturized luminometer for the bioluminescence assays Kamahori M, Harada K, Kambara H PM 01:30-02:15 ROOM 302 Poster Session #2, Discussion PM 02:15-03:00 ROOM 301 Plenary Lecture #5 (Chair: Nakashima K) Genetically Encoded Fluorescent and Bioluminescent Indicators for Cellular Signaling Pathways in Live Cells Umezawa Y PM 03:15-05:55 ROOM 301 Session #11 Basic and Applied Studies of Marine and Bacterial Bioluminescence (Chairs: Lee J, Karatani H) PM 03:15-03:40 (keynote lecture) Spectral modulation of bioluminescence from Vibrio fischeri strain Y1cells Karatani H, Yoshizawa S, Hirayama S PM 03:40-03:55 NO in photophores of bioluminescent fish Krönström J, Holmgren S, Baguet F, Mallefet J PM 03:55-04:10 Patterns and chemistry of siphonophore bioluminescence Haddock SHD PM 04:10-04:25 Ca2+-regulated photoproteins of the bioluminescent ctenophores: Cloning, expression, and some properties Markova SV, Burakova LP, Golz S, Frank LA, Vysotski ES PM 04:25-04:40 Studies on the bioluminescent mechanism of symplectin photoprotein Isobe M, Matsuda T, Kuse M, Mori H, Fujii T, Kondo N, Kageyama Y PM 04:40-04:55 Pharmacological and electrophysiological studies of light emission in 3 ophiuroid species: preliminary results Vanderlinden C, Nilius B, Gailly P, Mallefet J PM 04:55-05:25 (keynote lecture) The crystal structure of a Ca2+-discharged photoprotein supports the proton relay mechanisms proposed for the calcium trigger and bioluminescence Deng L, Markova SV, Vysotski ES, Liu ZJ, Lee J, Rose J, Wang BC PM 05:25-05:40 Structure based spectral tuning of photoprotein bioluminescence Vysotski ES, Stepanyuk GA, Markova SV, Malikova NP, Frank LA, Lee J

PM 05:40-05:55 PhotinaTM: an improved Ca2+-sensitive photoprotein Mastroianni N, Foti M, Bovolenta S, Stucchi M, Rossignoli A, Corazza S PM 03:15-06:00 ROOM 303 Session #12 Development in Biomedical Fields II (Chairs: Roda A, Totani M) PM 03:15-03:30 Development and biological application of a novel fluorescent probe for ratiometric imaging of protein tyrosine phosphatase activity Kikuchi K, Takakusa H, Nagano T PM 03:30-03:45 Effect of anaesthesia with propofol and remifentanil on whole-blood chemiluminescence: discriminant analysis of the results De Sole P, Rossi C, Scatena R PM 03:45-04:00 A homogenous bioluminescence assay for rapid microbial detection Butler B, Wood K, Fan F PM 04:00-04:15 Quantitative analysis of chemiluminescence intensity and toxicity in silico Hanai T, Tachikawa T PM 04:15-04:30 Capillary electrophoresis with chemiluminescence detector -Direct analysis of biomolecules- Tsukagoshi K, Nakahama K, Nakajima R (Chairs: Kai M, Nozaki O) PM 04:30-04:45 Determination of plasma catecholamine analysis with HPLC-peroxyoxalate chemiluminescence reaction detection system in mouse Tsunoda M, Imai K PM 04:45-05:00 Photosensitive luminol releasing compounds Nakazono M, Zaitsu K PM 05:00-05:15 Development of the enzyme immunoassay using new chemiluminescence substrate Yamada M, Matsumoto M, Watanabe N PM 05:15-05:30 Dual-antibody sandwich format combined with enzyme-linked immunomagnetic chemiluminescence for the detection of Escherichia Coli O157:H7 Gehring AG PM 05:30-05:45 Development and validation of a avidin-biotin chemiluminescence enzyme immunoassay (CL-EIA) for the quantitative detection of albumin in urinary Zhao L, Lin J-M, Wei Y, Li Z, Ma S

PM 05:45-06:00 Calcium-regulated photoprotein obelin as a label in immunoassay: An outlook for application Frank LA, Borisova VV, Vysotski ES PM 03:15-04:55 ROOM 304 Session #13 Functional Chemi-Luminophore (Chairs: Suzuki N, Kimura M) PM 03:15-03:35 Preparation of a crown-ether-modified isoluminol derivative and its chemiluminescence properties in an organic medium Okamoto H, Kimura M PM 03:35-03:55 Solid surface enhancement effects on chemiluminescence: Investigation of high performance solid media and its application to analytical chemistry Yoshinaga T, Ichimura T, Hiratsuka H PM 03:55-04:15 Inhibition and enhancement of luminol chemiluminescence by organic compounds Cui H, Xu H, Shi MJ, Zhou J PM 04:15-04:35 Excited states of dioxins as studied by ab initio quantum chemical computations: Anomalous luminescence characters Koto T, Toyota K, Sato K, Shiomi D, Takui T PM 04:35-04:55 Isoluminol chemiluminescence detection of reducing sample using ligand exchanging effect Niina N, Kodamatani H, Saito K, Yamazaki S PM 04:55-06:15 ROOM 304 Session #14 Singlet Oxygen and Superoxide (Chairs: Hirano T, Trofimov AV) PM 04:55-05:15 Purification of environment by singlet oxygen Suzuki N PM 05:15-05:35 Superoxide or singlet oxygen: the chemiluminescence of Cypridina luciferin analogues in the photodynamic solutions Bancirova M, Snyrychova I PM 05:35-05:55 On the role of singlet oxygen dimol in chemiluminescence of dioxirane reactions Kazakov VP, Kazakov DV, Latypova RR, Maistrenko GY, Mal'zev DV, Safarov FE PM 05:55-06:15 Universal chemiluminescent assay for oxidative and antioxidative processes in chemical and biological media: Fundamentals and application aspects Belyakov VA, Fedorova GF, Naumov VV, Trofimov AV, Vasil'ev RF

PM 06:30-08:30 "Pacific" room, InterContinental Hotels YOKAHAMA GRAND IC BANQUET All participants are welcome. Banquet will be held at 3F "Pacific" room, InterContinental Hotels YOKAHAMA GRAND IC near the symposium venue. The DeLuca Prize winners will be announced at the banquet. DAY 5 (Fri, Aug 6) AM 09:00-12:00 ROOM 301 Educational Session Scientific Educational Symposium for Early Teens Firefly Sheds Light on New Technology Organized by: The Organizing Committee of ISBC2004 Supported by: Kanagawa Prefecture Board of Education Yokohama City Board of Education Presented by: Kikkoman Corporation

POSTER PRESENTATIONS Poster Session #1 ROOM 302

Setup: Aug 2, PM 03:00-06:00 Aug 3, AM 08:00-09:00 Begins: Aug 3, AM 09:00 Discussion: Aug 3, AM 11:00-12:00 Ends/Take down: Aug 4, AM 10:00

A: Bioluminescence: Evolutional, Biological and Ecological Aspects 1-1 Bioluminescence: adaptive or defensive mechanism

Ali M

1-2 Bioluminescence and mating behaviour in pony fish, Leiognathus nuchalis Azuma N, Furubayashi C, Shichiri T, Wada M, Mizuno N, Suzuki Y

1-3 The first deep-sea observations of natural benthic bioluminescence using an in situ ISIT camera Battle EJV, Bagley PM, Priede IG

1-4 The diversity of bioluminescent beetles in the Atlantic Rain Forest (Brazil) Viviani V, Bechara EJH, Costa C

1-5 Bioluminescence on the web: The digital photobiology compendium Lee J, Branchini B, Guardigli M, Haddock S, Lin L, Meighen E, Mirasoli M, Pasini P, Roda A, Thomson C, Viviani V, Vysotski E, Ward W

1-6 "BIOLUMBASE" - the electronic collection of bioluminescent organisms Medvedeva SE, Boyandin AN, Lankin YuP, Kotov DA, Kargatova TV, Rodicheva EK, Popova LYu

1-7 Modules in emerging fields: An overview of bioluminescence Southworth TL, Gonzalez S, Michelini E, Roda A, Branchini BR

1-8 Possible mechanisms of luminous bacteria adaptations to environment. Vydryakova GA

1-9 Supplements for photobacterium phosphoreum RL-1 culture medium to enhance the luminescence activity Yu R, Imada C, Wada M, Kobayashi T, Hamada-Sato N, Watanabe E

B: Chemiluminescence 1-10 Synthesis and application of boronic acid derivatives of firefly luciferin to chemi- and

bioluminescent detection of peroxide Akhavan-Tafti H, Lauwers KS, de Silva R, Handley RS, Schaap AP

1-11 Toward blue-shifted firefly bioluminescence by the modification of the luciferin structure Amano Y, Ohkita Y, Nakamura M, Maki S, Hirano T, Ohmiya Y, Niwa H

1-12 Solvent effects on the chemiluminescence of TCPO in presence of 7-amino-4-trifluoromethylcoumarin Chaichi MJ, Shamsipour M, Karami A, Alizadeh K, Nazari O

1-13 Chemiluminescent detection of peroxide using novel stabilized 1,2-dioxetane boronic acids and boronate esters Eickholt RA, de Silva R, Handley RS, Schaap AP, Akhavan-Tafti H

1-14 A spectrophotometry of luminescence for quantitative spectral analysis Enomoto T, Ando Y, Yamada N, Irie T, Kubota H, Akiyama H, Ohmiya Y

1-15 Synthesis and electrochemistry of 5, 5'-dimethyl [12, 12'] bibenzo [b] acridylidene Fukai T, Kimura M

1-16 Molecular recognition for charge-transfer-induced chemiluminescent decomposition of optically active dioxetanes bearing a bisnaphthyl moiety triggered with an optically active crown ether / potassium t-butoxide Matsumoto M, Hamaoka K, Takashima Y, Yokokawa M, Watanabe N, Ijuin H K

1-17 On the mechanism of arylboronic acid "enhancement" of peroxidase-catalyzed reactions Handley RS, Eickholt RA, Schaap AP, Akhavan-Tafti H

1-18 Chemiluminescence reaction of 4-styrylphthalhydrazides. Remarkable substituent effect on the emitting species and chemiluminescence efficiency Motoyoshiya J, Yokota K, Hotta M, Nishii Y, Aoyama H

1-19 Studies on the chemiluminescence mechanism of Cypridina luciferin analogues: Dissociation constants of the singlet-excited Cypridina oxyluciferin analogues Saito R, Iwasa E, Katoh A

1-20 Chemiluminescence in the reactions of uranium and lanthanides Kazakov VP, Ostakhov SS, Kazakov DV, Mamikin AV, Antipin VA, Klimina SN, Khazimullina LN, Kochneva OA

1-21 Generation of chemiluminescence upon reaction of phenyltin (IV) compounds irradiated with UV light and tris (2,2'-bipyridine) ruthenium (III) Kodamatani H, Ogino A, Yokota K, Niina N, Muromatsu A, Fukushi K, Yamazaki S, Saito K

1-22 The high energy key intermediates in the peroxyoxalate chemiluminescence of 2,4,6-trichlorophenyl N-aryl-N-tosyloxamates Koike R, Motoyoshiya J, Nishii Y, Aoyama H

1-23 Chemiluminescence studies on the photochemical production of hydrogen peroxide from porphyrins and their aggregates Komagoe K, Osada S, Shindo T, Tamagake K

1-24 The chemiluminescence reaction mechanism of imidazopyrazinones: Effects of the reaction conditions for the chemiluminescence Kondo H, Maki S, Niwa H, Hirano T

1-25 Electron-excited states and energy transfer processes in bioluminescent reactions of luminous organisms Kudryasheva NS, Nemtseva EV, Kirillova TN, Belogurova NV, Sizykh AG

1-26 Lophineperoxides as an efficient organic source of singlet oxygen Kimura M, Lu G, Iga H, Nishikawa H

1-27 Synthesis and bioluminescent activities of the Latia luciferin analogs Masaki M, Mamino M, Nakamura M, Matsui R, Kojima S, Ohmiya Y, Maki S, Hirano T, Niwa H

1-28 Fluorescence and chemiluminescence characteristics of bisindoles Nakazono M, Asechi M, Zaitsu K

1-29 Synthesis and chemiluminescence of novel π-onjugated bisimidazopyrazinone derivatives Niibe M, Obata S, Maki S, Niwa H, Hirano T

1-30 A novel method to enhance the subcutaneous detection of bioluminescence in the facultative anaerobe, streptococcus pyogenes, by DMSO-assisted transdermal oxygen delivery Buxton DE, Childers BJ, Oberg KC

1-31 Solvent effect on the NMR and absorption spectra of firefly luciferin in Tetrahydrofuran Odai K, Nishiyama S, Shibata R, Yoshida Y, Wada N

1-32 High pressure effects on green fluorescent protein (GFP) chromophore models Okamoto T, Fujimori K, Niwa H

1-33 6,8-Diarylimidazo [1,2-a] pyrazin-3 (7H)-ones as potential chemiluminescent pH / superoxide double sensors Saito R, Suga N, Katoh A, Maki S, Hirano T, Niwa H

1-34 Improved polymer-supported photosensitizers Sandison MD, Larkin RK, Schaap AP, Akhavan-Tafti H

1-35 Substituent effect on regioselectivity of ene-reaction of lophine derivatives with singlet oxygen Wada J, Kimura M

1-36 Robust chemiluminescent peroxidase substrates Xie W, de Silva R, Eickholt RA, Mazelis ME, RA, Handley RS, Schaap AP, Akhavan-Tafti H

1-37 Color modulation for chemiluminescence of a dioxetane bearing a 3-aryl-5-hydroxyphenyl moiety triggered by potassium tert-butoxide / crown ether complex Matsumoto M, Kasai D, Yamada K, Fukuda N, Watanabe N, Ijuin HK

C: Basic and Applied Bioluminesence 1-38 Thermostabilization of firefly luciferase by using osmolytes

Eriksson J, Nyren P

1-39 Bioluminescence intensity modeling and optimization of sampling strategies Shulman I, McGillicuddy DJ, Haddock SHD, Moline MA, Phelps MW, Nechaev D

1-40 Ionic structure of the excited light-emitter in the calcium-activated photoprotein bioluminescence Mori K, Maki S, Niwa H, Hirano T

1-41 Molecular cloning and expression of a cDNA encoding the luciferase from the firefly, Lampyris turkestanicus Hosseinkhani S, Said Alipour B, Nikkhah M, Naderi-Manesh H, Sadeghi-Zadeh M, Naderi-Manesh M, Chaichi MJ

1-42 A novel orange fluorescent protein from the Cnidaria "tube anemone" Cerianthus sp. and its multi-color fluorescent mutants Ip DTM, Wan DCC

1-43 Chemical studies on bioluminescence of acorn worm, Ptychodera flava: Isolation and characterization of luminous substances Kanakubo A, Koga K, Isobe M

1-44 Biosynthesis of Cypridina luciferin from free amino acids in Cypridina (Vargula) hilgendorfii Kato S, Oba Y, Ojika M, Inouye S

1-45 The function of Trp residues in obelin bioluminescence Malikova NP, Vysotski ES, Blinks JR, Frank LA, Markova SV, Lee J

1-46 Chromatography and mass spectrometric analysis of isoforms of recombinant apoaequorin Masuda H, Takenaka Y, Mizuno H, Ohnishi-Kameyama M, Yoshida M, Tsuji FI

1-47 The bacterial bioluminescence as a protection from oxidative stress Remmel NN, Kratasyuk VA, Vydryakova GA, Labas YA

1-48 Spectral difference between obelin and aequorin is determined by the residue in position 88 Stepanyuk GA, Markova SV, Frank LA, Lee J, Vysotski ES

1-49 A MNDO-PM5 study of the enzyme-free NADH generation from NAD+ in the presence of electron-transfer mediator and an evaluation of the process based on bacterial bioluminescence Sugimoto T, Wada N, Karatani H

1-50 Effect of organic solvents on bioluminescence emission spectra of bacterial luciferase from Photobacterium leiognathi Sukovataya IE, Tyulkova NA

1-51 Kinetic investigation of bacterial Luciferase Mezhevikin VV, Sukovataya IE, Tyulkova NA

1-52 Spectral control of 2-arylimidazopyrazinone derivatives: substituent effect and an interaction with biological molecules Takamuki Y, Maki S, Niwa H, Hirano T

1-53 Biosynthesis of Vargula hilgendorfii luciferin, arisen from L-arginine, L-tryptophan, and L-isoleucine Toya Y

1-54 Luminous bacteria: applied aspects Vydryakova GA, Chugaeva YuV, Tyulkova NA, Medvedeva SE, Kuznetsov AM, Rodicheva EK

1-55 Bacterial bioluminescence with flavinmononucleotide activated by N- methylimidazole Krasnova OI, Tyulkova NA

1-56 Interaction of oxyluciferin's analogs, dimethyl oxyluciferin and monomethyl oxyluciferin, with firefly Luciferase Vlasova TN, Leontieva OV, Ugarova NN

1-57 Bacterial bioluminescence metabolic structure Dolgopolova YuB, Mezhevikin VV, Vydryakova GA

1-58 RFLP analysis of the luxA genes of Photobacterium leiognathi isolates derived from the symbiotic light organ of Leiognathid fish, Leiognathus rivulatus Wada M, Kamiya A, Kita-Tsukamoto K, Ikejima K, Nishida M, Kogure K

1-59 Development of novel bioluminescent reporter constructs for utilisation in Mycobacterial sp. Wiles S, Ferguson K, Robertson BD, Young DB

1-60 Phylogenetic analysis of dinoflagellate luciferase genes from seven species: a possible role for conserved nucleotides in the circadian regulation of protein synthesis Liu L, Hastings JW (Abstract in this book)

Poster Session #2 ROOM 302

Setup: Aug 4, AM 10:30-12:00 Aug 5, AM 08:00-09:00 Begins: Aug 5, AM 09:00 Discussion: Aug 5, PM 01:30-02:15 Ends/take down: Aug 6, AM 11:00

D: Development in Environmental Fields 2-1 Bacterial rainbow for microbial ecology studies

Brovko LY, Wang H, Kruis M, Griffiths MW

2-2 Control of microorganisms by singlet oxygen Fujimura A, Toshitoku Y, Suzuki N, Sato H, Kanazawa R

2-3 Chemiluminescence in the Ru(bpy)32+-catalyzed Belousov-Zhabotinsky reaction

Jinguji M, Ishihara M, Nakazawa T

2-4 Determination of particle-associated nitro-PAH using HPLC/chemiluminescence detection system Kameda T, Inazu K, Hisamatsu Y, Takenaka N, Bandow H

2-5 Simultaneous determination of twenty-one mutagenic nitropolycyclic aromatic hydrocarbons by high-performance liquid chromatography with chemiluminescence detection Tang N, Taga R, Hattori T, Toriba K, Kizu R, Hayakawa K

2-6 Luminol-dependent chemiluminescence of peripheral neutrophils from workers exposed to low frequency electromagnetic fields Vuotto ML, Sannolo N, Liotti F, Miranda R, De Seta C, Di Grazia M, Ruggiero G, Spatuzzi D, Sarno C, De Sole P

E: Development in Biomedical Fields 2-7 Homogeneous chemiluminescent assays: selective quenching of acridinium tracers

Adamczyk M, Fino JR, Mattingly PG, Johnson DD, Moore JA, Pan Y

2-8 Tandem bioluminescent enzyme immunoassay for BDNF and NT-4/5 Akahane S, Ito K, Arakawa H, Maeda M

2-9 A new assay for determining pyrophosphate using pyruvate phosphate dikinase and its application to DNA analysis Arakawa H, Karasawa K, Suzuki S, Maeda M

2-10 The chemiluminescent measurement of the black and green tea antioxidant capacity and the comparison with their antimicrobial activity Bancirova M, Medkova J

2-11 Method for implementing bioluminescence-based analytical assays in nanoliter volumes Bartholomeusz DA, Davis RH, Andrade JD

2-12 Use of the peroxyoxalate chemiluminescent reaction in acetone in the presence of Nile red for the analysis of glucose Castro-Hartmann P, Guerrero S, Daban J-R

2-13 Applications of Lumigen PS-atto and TMA-6 chemiluminescent peroxidase substrates de Silva R, Xie W, Sugioka K, Handley RS, Schaap AP, Akhavan-Tafti H

2-14 Use of new chemiluminescent reagent in detection of oxidase enzymes and their substrates by a coupled enzyme reaction de Silva R, Handley RS, Schaap AP, Akhavan-Tafti H

2-15 Induction of differentiation in HL-60 leukemia cells by HEMA, TEGDMA and Bis-GMA Nocca G, De Sole P, Gambarini G, Chimenti C, De Palma F, Giardina B, Lupi A

2-16 Application of enhanced chemiluminescence for immunochromatographic rapid assay of fatty acid binding protein (FABP) Egorov AM, Rubtsova MYu, Grigorenko VG, Andreeva IP

2-17 Immobilization of bioluminescent systems and their applications Esimbekova EN, Kratasyuk VA

2-18 Chemiluminescent analysis of the antioxidant and immunomodulation effects of several psychotropic drugs on peritoneal macrophages Hadjimitova V, Traykov T, Bakalova R, Petrova V, Lambev I, Ishikawa M, Baba Y

2-19 Highly sensitive CLEIA for C-peptide in serum with chemiluminescent substrate using a new CLEIA system Hayama S, Moriyama K, Kitajima S (Abstract in this book)

2-20 Chemiluminescence assay for lipase activity in human serum by using a proenhancer substrate Ichibangase T, Hamabe C, Ohba Y, Kishikawa N, Nakashima K, Kuroda N

2-21 Effect of caloric restriction and aging on the generation of reactive oxygen species in rat liver mitochondria and peroxisome Imada I, Sato EF, Konaka R, Nishikawa M, Kira Y, Park AM, Li Q, Inoue M

2-22 Development of tandem bioluminescent enzyme immunoassay for Angiotensin I and Endothelin-1 Ito K, Ohwaki K, Arakawa H, Maeda M

2-23 Determination of basic compounds with peroxyoxalate chemiluminescence detection Kawanishi H, Tsumura M, Fukushima T, Kato M, Toyo'oka T

2-24 Effects of forced exercise started from different ages on chemiluminescent response and cytokine excretion of alveolar macrophage Kumae T, Arakawa H

2-25 Determination of artemisinin by HPLC with on-line photoreactor and peroxyoxalate chemiluminescence detection Kuroda N, Amponsa-Karikari A, Kishikawa N, Ohba Y, Nakashima K

2-26 Effects of various antifungal agents on reactive oxygen species generation by Candida albicans Masui S, Majima T

2-27 Can hydrogen peroxide be generated from natural antimicrobial materials? Matsuo N, Someya K, Ueda Y, Arakawa H, Maeda M

2-28 HPLC with fluorescence detection of morphine in rat plasma using 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride as a label Nakashima K, Ogata Y, Nakashima MN, Wada M

2-29 Rapid and simultaneous bioluminescent assay using of aequorin and firefly Luciferase Nishimura W, Ito K, Arakawa H, Maeda M, Inouye S, Tatsumi H

2-30 Comparative study of ROS scavengers in terms of quenching MCLA-dependent chemiluminescence Obuki M, Nakajima J, Suzuki M, Miyahara K, Hosaka S

2-31 Specific detection of singlet oxygen using vinylpyrene derivatives as chemiluminescent probe Ohno K, Haryu Y, Nakano K, Lin J-M, Yamada M

2-32 Oxidative processes and catalytic properties of cytoplasmatic NAD-malate dehydrogenase in rat heart at norm and under ischemia Safonova OA, Popova TN, Matasova LV, Pinheiro de Carvalho MAA

2-33 Oxidative and glucose-6-phosphate dehydrogenase (G6PDH) activities in rat liver and blood serum at norm, toxic hepatitis and melatonin treatment Pashkov AN, Popov SS, Semenikhina AV, Safonova OA, Pinheiro de Carvalho MAA, Andreesheva EM

2-34 Development of a range of bioluminescent food borne pathogens for assessing in-situ heat inactivation and recovery of bacteria during heat treatment of foods Baldwin A, Nelson SM, Lewis RJ, Dowman A, Salisbury VC

2-35 Simultaneous measurement of fluorescence and chemiluminescence using neutrophil-like culture cells Satozono H, Kazumura K, Okazaki S, Hiramatsu M

2-36 Modeling of the signal intensity in the various reaction chamber of the NO-O3 chemiluminescence nitrogen oxides monitor to obtain higher sensitivity Sawada H, Okitsu K, Takenaka N, Bandow H

2-37 Chemiluminescent study on oxidation of mono-, di- and poly-saccharides Tamefusa T, Kimura J, Ito R, Inoue K, Yoshimura Y, Nakazawa H

2-38 Study on chemiluminescent probes for superoxide anions - control of chemiluminescence resonance energy transfer by cyclomaltooligosaccharide (cyclodextrin) - Teranishi K, Nishiguchi T

2-39 Development of FIA-chemiluminescence methods to evaluate quenching effects against reactive oxygen species Wada M, Katoh M, Kido H, Nakashima MN, Kuroda N, Nakashima K

2-40 Bioluminescent detection of RNA hydrolysis probes in DNA testing White PJ, Gandelman O, Tisi L, Murray JA, Lee MA, Squirrell DJ

2-41 Development of highly sensitive analysis of glycated protein in human hair by luminol chemiluminescence Yajima T, Ito K, Ito R, Inoue K, Masubuchi K, Yoshimura Y, Yamada S, Atsuda K, Kubo H, Nakazawa H

2-42 Development of a new chemiluminescence substrate for the enzyme immunoassay Yamada M, Kitaoka K, Matsumoto M, Watanabe N

2-43 Syntheses and properties of cell-membrane permeable lucigenin derivatives for the assay of intracellular superoxide Yamada S, Kohsaka N, Iwamura M

2-44 Chemiluminescent immunometric detection of SARS-CoV in sera as a early diagnosis of SARS Yang X, Sun X

F: Biological Application with Fluorescent Biomolecules and Cells 2-45 Use of bioluminescent Salmonella typhimurium DT104 to monitor uptake and intracellular

survival within a human cell-line Angell JE, Salisbury VC, Hill PJ, Alloush HM

2-46 Multi-parametric experimental design of bioluminescence-based analytical assays Al-Sheikh Y, Abernathy J, Bartholomeusz DA, Davis RH, Andrade JD

2-47 Design and synthesis of luminescent lanthanide complexes with a luminescence OFF/ON switch Kikuchi K, Iwasawa S, Nagano T

2-48 Visualization of superoxide generated from colonies of Candida albicans Masui S, Majima T, Ito-Kuwa S, Nakamura K, Aoki S

2-49 Single-molecular imaging of protein in living cell by pin-fiber video-microscope Hirakawa Y, Hasegawa T, Masujima T, Tokunaga M, Tsuyama N, Kawano M

G: Luminescent Bio-imaging and Biosensors 2-50 Construction of a novel bioluminescence bacterial biosensor for real-time monitoring of

cytotoxic drugs activity Alloush HM, Angell JE, Smith MA, Perehinec TM, Hill PJ, Salisbury VC

2-51 Chemiluminescent microspheres for measuring reactive oxygen species (ROS) in phagocytosis Hosaka S, Hosaka Y, Ichimura K

2-52 Pre-treatment of plant activator enhances the elicitor-responsive photon emission from the rice cell culture. Iyozumi H, Kato K, Kageyama C, Inagaki H, Furuse K, Baba K, Tsuchiya H (Abstract in this book)

2-53 Potential mechanism of the enhancement of photoluminescence of CdSe quantum dots under UV-irradiation: role of free Cd and Se ions Zhelev Z, Jose R, Bakalova R, Nagase T, Ohba H, Ishikawa M, Baba Y

H: Industrial Application of Firefly Luciferase 2-54 Bioluminescent assay of hygiene quality of ground beef

Froundjian VG, Ugarova NN, Moroz NA

2-55 Bioluminescent assay of sterility or cleanliness in hospital environment Froundjian VG, Ugarova NN, Gabriyelyan NI, Aref`eva LI, Preobrazhenskay TB

2-56 Rapid detection of microorganisms in aseptic products using an ATP bioluminescent system Igarashi T (Abstract in this book)

2-57 A new instrument for automated luminescent assays Symonds WH, Squirrell DJ, Jackson RS

2-58 Hospital testing of a rapid bioluminescent assay for MRSA Squirrell DJ, Murphy MJ, Leslie RL, Matthews SCW, Cotterill SL, Skyrme M

2-59 A single-step bioluminescent endpoint assay for Nucleic Acid Amplification Gandelman O, Church V, Murray J, Tisi L

SESSION ORGANIZERS A: Basic and Applied Bioluminescence: Luciferase, Photoprotein and Fluorescence-Protein

Organizers: Ohmiya Y, Viviani V Session #8 (ROOM 301, Thu, Aug 5) Session #11 (ROOM 301, Thu, Aug 5)

B: Chemiluminescence: Proving into Secrets in Chemiluminescence

Organizers: Kimura M, Fujimori K Session #1 (ROOM 301, Tue, Aug 3) Session #2 (ROOM 301, Tue, Aug 3) Session #7 (ROOM 304, Wed, Aug 4) Session #13 (ROOM 304, Thu, Aug 5) Session #14 (ROOM 304, Thu, Aug 5) Bioluminescence Chemiluminescence Festival Plaza (ROOM 304, Wed, Aug 4)

C: Bioluminescence: Evolutional, Biological and Ecological Aspects Organizers: Ohmiya Y, Viviani V

Session #6 (ROOM 303, Wed, Aug 4) D: Recent Development in Environmental Fields

Organizers: Hayakawa K, Nakashima K, Kuroda N Session #4 (ROOM 304, Tue, Aug 3,)

E: Development in Biomedical Fields

Organizers: Nozaki O, Totani M, Santa T Session #9 (ROOM 303, Thu, Aug 5) Session #12 (ROOM 303, Thu, Aug 5)

F: Biological Application with Fluorescent Biomolecules and Cells

Organizer: Tsujimoto K Session #5 (ROOM 301, Wed, Aug 4)

G: Luminescent Bio-imaging and Biosensors Developed with Nanomolecules and Nanoparticles

Organizers: Hayakawa K, Tamiya E, Tsujimoto K Session #3 (ROOM 303, Tue, Aug 3)

H: Frontiers of Industrial Application of Firefly Luciferase

Organizers: Hattori N, Matsuyama A Session #10 (ROOM 304, Thu, Aug 5)

Late and Corrected Abstract

Highly sensitive CLEIA for C-peptide in serum with chemiluminescent substrate using a new CLEIA system Hayama S, Moriyama K, Kitajima S Fujirebio Inc., Research and Development Division, 51 Komiya-cho Hachioji, Tokyo 192-0031, Japan Background: Measurement of C-peptide in serum is useful for presumption of pancreatic β-cell function, preventing influence of exogenous insulin or insulin autoantibody. It has been thought that it is difficult to measure without being influenced of proinsulin. Moreover, a C-peptide is small molecule it has been thought that it is difficult to measure using two monoclonal antibodies. Therefore we have developed the highly sensitive and specific assay for C-peptide in serum and plasma on the LUMIPULSE system. The system is a fully automated chemiluminescent enzyme immunoassay (CLEIA) system, that uses AMPPD as a substrate for alkaline phosphatase and ferrite micro-particles as a solid phase. Methods: Utilizing a monoclonal antibody that recognizes N-terminal of the C-peptide molecule realized the specificity of the assay. It is measured correctly, without influencing C-peptide of the molecule used as proinsulin or the fragmentized C-peptide by the singularity of this antibody. The assay format for C-peptide was based on 2-step sandwich principle. The incubation times for first and second reactions were 10 minutes each. The amount of detecting antibodies bound on the particle were determined by measuring chemiluminescent light emission of the AMPPD substrate. Results: The evaluation was performed based on the NCCLS guideline. Representative data from some of the assay are summarized in the table below: Detection interval 0.02 - 30ng/mL Imprecision (CV) 2.64% (2.40ng/mL)

1.62% (8.45ng/mL) Limit of detection 0.004ng/mL Limit of quantitation 0.02ng/mL Cross-reactivity pro-insulin, 0.6% Conclusions: The C-peptide assay on the system showed good sensitivity, specificity and

precision. Accordingly it was concluded that the C-peptide assay was of use to diagnose glucose metabolic disorder and to monitor the disease status of diabetes. Rapid detection of microorganisms in aseptic products using an ATP bioluminescent system. Igarashi T Research & Development Division, Kikkoman Corporation, Chiba 278-0037, Japan. Using a mutant firefly luciferase (heat-resistant and surfactant tolerant) and an ATP degradation system, we have developed a rapid method to monitor aseptic conditions in food and drink products. We used three bacteria strains of Pseudomonas fluorescens, Bacillus mycoides and Klebsiella pneumoniae, which were isolated from non-sterility products. Each of these bacteria were cultured in dairy creams or cocoa drink for 24 h at 37 °C and then an each aliquot of each cultured sample was assayed using our new ATP bioluminescent kit and the traditional plating method. Using our new ATP kit, the detection limit of P. fluorescens, B. mycoides and K. pneumoniae were 2x104, 1x103and 5x103 CFU/ml, respectively. This ATP system was widely used at food plants (cream, milk and yogurt) and beverage plants (canned coffee) in Japan. Pre-treatment of plant activator enhances the elicitor-responsive photon emission from the rice cell culture. Iyozumi H1, Kato K1, Kageyama C1, Inagaki H1, Furuse K2, Baba K3 and Tsuchiya H4

1. Shizuoka Agricultural Experiment Station, 678-1, Tomigaoka, Toyoda, Iwata, Shizuoka 438-0803, Japan 2. Kumiai Chemical Industry Co., Ltd., 3360, Kamo, Kikugawa, Ogasa, Shizuoka 439-0031, Japan 3. Nippon Soda Co., Ltd., 345, Takada, Odawara, Kanagawa 250-0280, Japan 4. Hamamatsu Photonics K. K., 5000, Hirakuchi, Hamakita, Shizuoka 434-8601, Japan

Plants generate a relatively high level of ultraweak photon emission during their defense response to pathogens. The spectral composition of the photon emission is also different from that of their steady state. These phenomena could be indicators of physiological changes in a defense response. Plants also show a defense response related photon emission against the “elicitor”. Elicitors are the substances of biological and non-biological origin that induce defense responses in plants or in cell cultures. The cell culture-elicitor system is often used as a simplified model of defense responses of many plants. Recently, new types of agrochemicals termed “plant activators” have been used. These chemicals are eco-friendly because they protect plants from diseases by intensifying plants’ own defense response, while they do not have germicidal activity. A rice cell culture was pre-treated with a plant activator and then was mixed with an elicitor. Pre-treatment of acibenzolar-S-methyl, probenazole, methyl jasmonate, and carpropamide, that intensify defense response in rice, increased the intensity of the elicitor responsive photon emission by two times or higher. The plant activators themselves did not induce such a remarkable photon emission from rice cells. The initial rise of the increased photon emission became faster when the pre-treatment period became longer. The spectral composition of the increased photon emission was quite similar to that of the non-enhanced elicitor responsive photon emission. The results indicate that the pre-treatment of plant activators enhance the elicitor-responsive photon emission from the rice cell culture. A powerful screening method for plant activators is presumably derived from the findings. Structural Basis of firefly luciferase reaction Hiroaki Kato Graduate School of Pharmaceutical Sciences, Kyoto University, and RIKEN Harima Institute at SPring-8 Enzymes undergo distinct structural changes during their reaction or as a control of their reaction. A fundamental understanding of enzyme mechanism must therefore include a description of structural changes during the reaction. Firefly luciferase catalyzes an

oxidative reaction involving ATP, firefly luciferin and molecular oxygen, yielding an electronically excited oxyluciferin species. This excited species emits yellow-green visible light. To capture transit species in each step during the enzymatic reaction, an analogue of luciferyl-AMP intermediate of the reaction has been synthesized, and the products of the reaction have been obtained by the enzymatic reaction. The crystal structures of the enzyme complexed with them were solved at 1.3 and 1.6 Å resolutions, respectively. In this talk, I will describe the catalytic machinery of the reaction based on the structures. The use of ATP bioluminescence for monitoring biocide or disinfectant treatment of water Ramsay CM, Wayman D, Davenport K, Michie I Biotrace Limited, The Science Park, Bridgend, CF31 3NA There are many industrial processes where control of microbial contamination is important to maintain the quality and/or safety of water systems; chemical biocides are generally used for this purpose. ATP bioluminescence measurement is a commonly used technique to monitor the effectiveness of the biocide treatment of waters in cooling towers, power stations, oil and gas recovery and in paper pulp processing. It is known that the results very much depend on the nature of the biocidal mode of action and that measurement of ‘free’ or non cellular ATP can be of use in interpreting the results. We have evaluated biocides of different types and have data that illustrates the different patterns of results that might be seen. New EU directives may limit the development and application of new biocides and for this reason, and other concerns such as over pollution, there is interest in alternative water treatment techniques. The use of ATP measurements to monitor the effectiveness of electro-chemical treatment has been evaluated in our laboratory. The results indicate that as with chemical biocide treatment the use of both Total and Free ATP measurements can be of value in the interpretation of the results and monitoring the efficacy of this method of water treatment.

Bioluminescence reaction in the firefly squid, Watasenia scintillans Frederick I. Tsuji Marine Biology Research Division, S.I.O., Univ. of California at San Diego, La Jolla, CA. 92093-0202, U.S.A. Each spring, in a far-off region of northern Japan, where the Noto Peninsula projects into the Sea of Japan forming Toyama Bay (maximum depth, ca. 1,200 meters), the deep-sea squid Watasenia scintillans (mantle length, ca. 6 cm; wet weight, ca. 9 gm), comes inshore to lay fertilized eggs. The migration takes place on a vast scale, involving hundreds of millions of squids. The migration is most notable in Toyama Bay. For nearly a hundred years the squid has served as a food source and a local fishery has developed around the squid in the small maritime city of Namerikawa. The squids are caught by setting nets in shallow water and in March-April; of this year, the recorded catch was 2,500 tons. The squid has been studied ever since the luminescence was first described by Watasé in 1905, for whom the squid is named. An early visitor was E. Newton Harvey, who made the long journey from Princeton and reported his observations in a paper in 1917. The squid has more than 800 minute luminous organs distributed over its ventral mantle, a row of 5 prominent organs lining the lower margin of its eye and a cluster of 3 tiny pigmented organs (less than 1 mm diam) on the tips of the 4th pair of arms. The ventral organs produce a steady glow of light, whereas the arm organs emit brilliant flashes of light (470 nm). The rhythmic flashing sometimes observed in the arm organs resembles that of a firefly flashing at night and so the squid is known in Japan as the “firefly squid.” The Watasenia reaction is due to a luciferin-luciferase reaction, involving a “soluble component”, “insoluble component,” ATP, Mg ions and molecular oxygen. The optimum pH is 8.8. If the arm organs are homogenized in Tris-HCl buffer, pH 8.3, and injected with ATP, a bright luminescence is observed. On centrifuging the homogenate, the supernatant is found to contain luciferin as a “soluble component,” whereas the pellet yields an unstable membrane-bound luciferase as “insoluble component.” Neither the “soluble component” nor the “insoluble component” gives light with ATP, except when reconstituted. There is an absolute requirement

for molecular oxygen. Earlier work on structure determination, total chemical synthesis and measurement of light-emitting activity has shown that the luciferin is coelenterazine disulfate. Based on results with Watasenia and other luminescent systems, a hypothetical scheme for the Watasenia reaction is proposed involving: (1) a base/luciferase-catalyzed enolization of the C-3 keto oxygen of coelenterazine disulfate, (2) an adenylation of the enol group by ATP forming the intermediate adenyl colenterazine disulfate, (3) removal of AMP and the addition of molecular oxygen to the C-2 carbon forming a dioxetanone intermediate and (4) spontaneous cleavage of the dioxetanone ring yielding carbon dioxide, coelenteramide disulfate and 60 kcal/mol of energy required for the blue light emission. The probable light emitter in the reaction is the excited state amide anion of coelenteramide bound to luciferase. Phylogenetic analysis of dinoflagellate luciferase genes from seven species: a possible role for conserved nucleotides in the circadian regulation of protein synthesis Liu L, Hastings JW Dept of Molecular & Cellular Biology, Harvard University, Cambridge, MA 02138 USA The structures and sequences of luciferase genes from five bioluminescent species, Alexandrium affin, Alexandrium tamarensis, Pyrocystis noctiluca, Pyrocystis fusiformis, and Protoceratium reticulatum have been determined and compared with two previously reported ones from Lingulodinium polyedrum and Pyrocystis lunula. All have a structure that is unique to dinoflagellate luciferases: three homologous catalytic domains preceded by an N-terminal region of unknown function. Both pairwise comparison and phylogenetic inference indicate that the similarity of the corresponding individual domains between species is greater than that of the different domains within the polypeptide. Trees constructed from each of the three individual domains are congruent with the tree of the full-length coding sequence, suggesting coevolution of three domains. Luciferase and ribosomal DNA trees both indicate that the L. polyedrum luciferase represents the most primitive form. Synonymous

but not nonsynonymous substitution rates of the central region of the intramolecularly conserved domains vary considerably among the lineages, being greatly reduced from the flanking regions in L. polyedrum, mildly in Protoceratium and very little if any in Alexandrium and Pyrocystis species. The lineage-specific constraints of synonymous substitution in the central region of the domains correlate inversely with the content of GC3 (~87 % for Lingulodinium, 84% for Protoceratium, and only ~65% for Pyrocystis). The difference in GC3 content can be accounted for by the biased usage toward C-ending codons at the degenerate sites.

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Annex HallAnnex HallAnnex Hall

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NationalNationalConvention HallConvention Hall

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