$508 Friday, November 11, 2005 Poster Abstracts

pyramidal cells appear on layer V at the tenth day and on both layer V and III after culturing 12 days. In the control group, NFH positive pyranfidal cells appear on layer V in 5-day-old rats, and in over 3-week-old rats which appear on both layer V and III. In cultured cerebral slices, the number of pyramidal neurons in layer V of M1 area is invariable fi'om 12 days to 2 months. Conclusions: Orgaotypic cerebral culture can be used to study postnatal development of neocortex and build some in vitro models of neurodegenerative diseases.

1605 Tau protein, but not 14-3-3 protein, is elevated in rdapsing reurtting MS

Frederiksen JL 1, Kristensen K 1, Milthers ja, Chtistiansen M 2, Csarna, JM 3. ~ Department of Neurology, Glostrup Hospital, University o f Copenhagen, Denmark; 2Department o f clinical biochemistry and Department of Autoimmunity, Statens Serum Institute, Copenhagot, Denmark

Background: The progress in treatments possibilities in nmltiple sclerosis (MS) has increased the need of biomarkers to make an early diagnosis of MS and to monitor the disease course and the effect of treatment. Small, retrospective studies have suggested that Tau protein and 14-3-3 protein may be valuable biomarkers of mxonal damage in MS. Methods: Tau protein and 14-3-3 protein were analysed with the routine metod used in the department of autoimmmfity and the dep- artment of clinical biochemistry, respectively. The refence values of Tau protein was divided according to age: Below 40 years, 40-70 years and above 70 years. We studied 61 patients divided into relapsing- remitting (RR) MS (in -- 27) and acute ON (n - 34), either isolated or as part of MS. These results were correlated to the results IgG index and oligoclonal bands in the CSF and to findings on brain MRI. Results: The concentration of tau protein was significantly elevated in patients with RRMS and in patients with ON who went on to develop clinically definite MS, but not in patients with isolated ON. The concentrationen of Tau protein was significantly correlated to the EDSS score. The 14-3-3 proetin was not present in any patient. Conclusions: Increased concentration of tau protein may be of prognostic value for developing MS and Tau protein seems a useful marker to monitor neurolocal deficits in RRMS and may thereby correlate to the degree of mxonal skade. The relationship of Tau values with MRI and IgG status in the CSF findings will be presented at the congress. 14-3-3 protein cannot be used as a biomarker in MS.

1606 Multiple Sclerosis: evaluation of 100 cases: diagnosis, trea_hnent and evolution over the period of 1998 to 2003

Gomes, S 2, Luna, AST, Pizzatto, R, Valle, ELR, Dantas, TN, Campanella, CAD, Rodrigues, LC, Cassis, R, Sassine, S, Godoy, R, Oliveira, PGD, Montanaro, AC. ~Hospital Real e Benemdrita Sociedade Portuguesa de Ben@'cigncia HRBSPB; 2Faculdade de Medicina da Universidade de Sdo Paulo FMUSP, Sao Paulo, Brazil," 3Hospital Geral Exdreito de S~o Paulo - HGESP, Brazil

Objective: Clinical evaluation of the patients concerning sex, race, medium age, of the symptoms onset and ssanptoms. Methods: Clinical analysis, LCR, RNM, evoked potential and clinical evolution. Pulsetherapy with metil prednisolona and interferon B1A. Results: Female gender predominance, 20-40 age ranging, pyramidal symptoms/optical nerve/sensitive. Conclusion: The RNM works as a marker in the disease diagnosis.

1607 Isaacs Syndrome: ease report

Gomes, S 2, Pizzato, R, Valle, ELR, Dantas, TN, Campanella, CAD, Luna, AST, Rodrigues, LC, Cassis, R, Sassine, S, Godoy, R, Oliveira,

PGD, Montanaro, AC. 1Hospital Real e BenemOrita Soeiedade Portuguesa de Benefieidneia - HRBSPB; 2Faeuldade de Medieina da Universidade de Sdo Paulo FMUSP, Sao Paulo, Brazil," 3Hospital Geral Ex~rcito de Sdo Paulo HGESP, Brazil

Objective: Isaacs Syndrome neuro-physiological and clinical report. Methods: PAL, 35 years old, female, with a scenario of proximal muscular strength reduction in lower and upper limbs associated with cramps, muscular contractures. Presenting over exam, distal muscular atrophy of the upper limbs, generalized cereous hypertony, grade 3+ global nmscular strength, hyporeflexy and fasciculation presence. Results: Enzimatic disturbances, ENMG evidentiating spontaneous discharges of motor unity potentials, normal neuro-conduction, phar- macological proposal done with carbamazepina obtaining improve- ment in the scenario. Conclusion: The Isaacs Syndrome is an autoinmmne disease developed in the kation canals through the presence of potassium attticanals antibodies. Presence of continuous nmscular activity and myokymia. Response to fenitoine and carbamazepina.

1608 Tropical Spastic Paraparesis - Hamtsp: ease report

Gomes, S 2, Dantas, TN, Campanella, CAD, Luna, Pizzato, R, Valle, ELR, Rodrigues, LC, Cassis, R, Sassine, S, Godoy, R, Oliveira, PGD, Montanaro, AC. ~Hospital Real e Benem~rita Sociedade Portuguesa de Beneficidneia HRBSPB; 2Faculdade de Medieina da Universidade de Sdo Paulo - FMUSP, Sao Paulo, Brazil," 3Hospital Geral Ex~reito de S~.o Paulo - HGESP, Brazil

Objective: To give an account of medullar disease with retroviral etlfiology. Method: SAP, 60 years old, with lower limbs progressive motor deficit scenario for three years. Sphincteral disturbances presence. Presented paraparesis, overexdted reflexes, pallesthetic disturbances, pyramidal syndrome over exam. Positive serology for HTLVI, thoracic, cohinn RNM evidentiating T2-T9 levels increase. Condasion: Commentary on the tropical spastic myelopathies.

1609 Dis~nyelinating Polineuropathy in patient with Chrotdcal kidney ins~lfliciency

Gomes, S 2, Valle, ELR, Dantas, TN, Campanella, CAD, Luna, Pizzato, R, Rodrigues, LC, Cassis, R, Sassine, S, Godoy, R, Oliveira, PGD, Montanaro, AC. 1Hospital Real e Benem~rita Sociedade Portuguesa de Beneficigncia HRBSPB; 2Faculdade de Medicina da Universidade de Sdo Paulo FMUSP, Sao Paulo, Brazil," 3Hospital Geral Ex~reito de S~o Paulo - HGESP, Brazil

Objective: Report the response after use of gamaglobulin in chronical kidney patient. PC, 30 years old, with clinical scenario of IRC with kidney transplant and benefitting with hemodialysis. Developed flacid tetraparesis with hyporeflexy. During the neurophysiological investi- gation, presented sensitivemotor polineuropathy scenario with dismye- linating standard.

LCR evidentiates protein increase. Use of gamaglobulin preconized. Results: GamaglobulJn in the 24g/day dosis for five days with good response to the neurological symptoms. Conclusion: The sensitive, motor, mixed, autonomic neuropattfic process with mxonal characteristics and uremia increase is the mani- festation of what occurs in the chronical kidney insufidency. The incre- sase in the uremia complicates the diagnosis of the mxonal neuropathy. The neurophysiological study contributes greatly in the evaluation and the gamaglobulin in the improvement of the clinical scenario.

1610 Neurological Matfitestations of the Internfittent Acute Porphyria: Case Report