Poster Abstracts Friday, November 11, 2005 $515

Background: Vestibular evoked myogenic potentials (NEMP) have been known to useful in documenting abnormality in patients with various vestibular disorders but the studies of VEMP in stroke patients are rare. Method: We recorded VEMP in 23 consecutive patients with acute stroke. 21 patients had ischemic stroke and 2 patients had intracerebral hemorrhage. All patients underwent magnetic resonance imaging and we compare VEMP results with the lesion documented by brain imaging. VEMP were defined to be abnormal when they were very asyimnetrical (one is 2 times of more as large as the other), or absent in one side. The patients who showed bilateral no response in VEMPs were excluded from this study. Results: VEMP abnormalities were found in 23?/; (18/23). VEMP were abnormal in 147/; (2/14) in the patients with supratentorial lesions, 78% (7/9) in brainstem lesions. Most abnormalities found in the ipsilateral side of the lesion (8/10) but abnormalities in contralateral side of lesion were found in 50% (12/4) of patients with pontine infarction. Conclusion: VEMP would be considered a useful complementary neurophysiological tool for the evaluation of brainstem dysfunction in acute stroke patients. Supraneuclear control may play a role in the abnormalities found in supratentorial stroke patients.

I636 Hyperinstdinenfia of Myototfic Dystrophy Type 1 (Dml) might be Inhibited using Voglibose

Kinoshita M 1'2, [mai T z, Satoh M z, I toh T z, [soda K ~, Hasegawa H ~, Mitarai M 1, Tochigi S ~, Yonemoto K ~, Hirose K 4. 1Fourth Department of Internal Medicine, Saitama Medical Center, Saitama Medical School, Saitama, Japan; 2Saitama Yorii Hospital, Saitama, Japan; XTokyo Metropolitan University of Health Sciences, Tokyo, 3"apart; .4 Ueno Hospital, Tokyo, )'apart

Background: Hyperinsulinemia or diabetes mellitus has been occasion- ally shown in myotonic dystrophy type 1 (DMI). I hit on the idea that hyperinsulinemia of the disease might be inhibited using voglibose, which has the inhibitory actions on intestinal ~-glucosidase activity and postprandial hyperglycemia. Method: 11 DM1 patients with hyperinsulinemia were chosen. After being fasted for 15 hours, all patients had 500 kcal carbohydrate rich breakfasts. Shortly before and at 30, 60, 90, 120, and 180 min after the breakfast, blood samples were taken to measure both plasma glucose and insulin levels (voglibose minus group). After being fasted for 15 hours in another day, in these patients one tablet of vogli was administered shortly before the same breakfast. Similarly, blood samples were done (voglibose plus group). Southern blot analysis was done. Results: Abnormal expanded allele sizes in all patients were ranged from 0.6 to 4.3 kb. Both each level and total sum of plasma glucose were not significant between the above two groups. Plasma insulin30 level in voglibose plus group was significantly lower than that in voglibose minus group, whereas another insulin levels were not significant between the two groups. Total sum of insulin in voglibose plus group was significantly lower than that in voglibose minus group. The values and percent of the maxinmm reductions in insulin levels were 112.7 ± 71.7 pU/ml and 60.6 ± 13.3',/o. Conclusion: Hyperinsulinemia of DM1 was inhibited using voglibose and administration of that agent might prevent these patients with hyperinsulinemia from diabetes mellitus.

1637 Influence of Neuroprotectant Xymedon on Number of Different Populations DRG Neuxons During of Rats Development

Kozlova, M 1, Raginov, 11, Chelyshev, Yu 1. 1Kazan State Medical University, Kazan, Russia

We have previously demonstrated the xymedon is a potent neuropro- teeter to DRG neurons after injury. Xymedon support survival IB4 + and NF200 + neurons and stinmlate expression of atttiapoptotic protein Bcl-2 and regeneration of myelinic fibers. In the current study, we are the estimated of the total nmnber of D R G neurons, quantity of the small, medimn and large neurons populations in L5 DRG on post-natal day 15, with injection xymedon since P0 to P15 (30mg/kg i/p). The number of the small neurons are 55,4%, medium neu rons - 28,2% and large neurons - 15,8% of total number of DRG neurons in P0 rats. Total number of neurons on P15 is increased by 58"/0 (P < 0,05). As well as the nmnber of large neurons is increased by 57% (P < 0,05), medium neurons is unchanged, and number of small neurons is decreased by 13%. In xymedon group the total number of neurons is increased approximately 20%, in comparison with P15 (without xymedon). At the same time, the number of large, medium and small neurons is increased by 98"/0 (P < 0,05), 43% (P < 0,05) and 16',/o, respectively. We support, that neuroprotecter xymedon has stimulation effect on differentiation DRG neurons during development.

1638 Substantia Nigra Maxinesco bodies in the brains of individuals without Neurodegenerative Diseases

Krygowska-Wajs, A 1, Adamek, D 2, Kuter, K 3, Gryz-Kurek, E 1, Dec, M ~, Smialowska, M 4, Wolfarth S 3, Ossowska, K 3 . 1Department of Neurology Collegium Medicum, Yagiellonian University, Cracow, Poland.: 2Department of Neuropathology, Collegium Medieum Jagiellonian Univesity, Cracow, Poland," 3Department of Neuro- Psyehopharmaeology, Institute of Pharmacology, Polish Academy of Sciences, Cracow, Poland; 4Department of Neurobiology, Institute of Pharmacology, Polish Academy of Sciences, Cracow. Poland

Background: Marinesco bodies (MB) are nuclear inclusions found in pigmented neurons of the substantia nigra and locus ceruleus. Although the biological role of Marinesco bodies and other intra- cellular inclusions for neurodegeneration of dopamJnergic neurons are still unresolved, it has been found that the frequency of these inclusions negatively correlate with striatal concentrations of the dopanrinergic neuron markers: dopamJne transporter and tyrosine hydroxylase. MB were found in spinocerebellar atmxia type 3 and other trinucleotide repeated disorders and also in normal elderly brains. Method: We evaluated 20 brains of clinically normal individuals, who died of various causes but without apparent clinical signs of Parkinsonism/or Dementia and one parkJnsonian brain, hmnunocy- tochemical examination was performed using monoclonal antibodies against ~-synuclein, tau protein, ubiquitin and beta amyloid. Results: In series of normal individuals ubiquitin-positive and ~-synyclein negative MB were found in pigmented neurons of substantia nigra in 80% of brains studied. MB frequency increased with advancing age. There was no correlation between the frequency of substantia nigra MB and neurodegenerative changes of senile type. Numerous MB have been found in neurons of substantia nigra parkinsonian brain. Conclusion: Preliminary studies indicate that MB frequency increases with advancing age but no correlation has been found with morpho- logical changes of senile type; Further studies are needed to ascertain the molecular basis of MB. The study was supported by the State Committee for Scientific. Research (KBN) as the solicited research project PB2-MIN-001/PO5/18.

1639 Acquired copper deficiency mimicking Sub Acute Combined Degeneration of the Spinal Cord

Land, JM 1, Briddon, A 1, Worth, p1, Giovannoni, G 1 . ~Neurometabolic Unit, National Hospital for Neurology', London, United Kingdom