2-AB Labeled Glucose Homopolymer Ladder : Cat. No. CAB-GHP-x © Ludger Ltd, 2005 Page 1

2-AB (2-aminobenzamide) Labeled Glucose Homopolymer (GHP) Ladder

Cat. No. CAB-GHP-x (where x denotes pack size)

Structure [Glcα1-6]n Glc-2AB

Synonyms : 2-AB labeled dextran hydrolysate ladder

Description: 2-aminobenzamide labeled glucose homopolymer ladder for use as a calibration

standard for HPLC and MS analysis of glycans. The ladder contains glucose

homopolymer species from degree of polymerization (dp) 1 to up to dp 22 (i.e. the

glucose monomer GU1-2AB to GU22-2AB).

Preparation : Glucose homopolymer ladder was obtained by controlled acid hydrolysis of dextran

then labeled by reductive amination at the reducing termini with the fluorescent glycan

tag 2-aminobenzamide (2-AB).

Form: Dry. Dried by centrifugal evaporation from an aqueous solution.

Purity: > 90% pure as assessed by HPLC.

Use: See Certificate of Analysis for recommended sample dilution and injection volume.

Storage: Refrigerate (-20oC) both before and after dissolution. This product is stable for at least

5 years as supplied.

Shipping: The product can be shipped at ambient when dry. After dissolution, ship on dry ice.

Handling: Allow the unopened vial to reach ambient temperature and tap unopened on a solid

surface to ensure that most of the lyophilized material is at the bottom of the vial.

Gently remove the cap, add the desired volume of reconstitution medium, re-cap and

mix thoroughly to bring all the oligosaccharide into solution. We recommend

analytical grade pure water as the reconstitution medium. For maximal recovery of

oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the

2-AB Labeled Glucose Homopolymer Ladder : Cat. No. CAB-GHP-x © Ludger Ltd, 2005 Page 2

reconstituted vial briefly before use. Ensure that any glass, plastic ware or solvents

used are free of glycosidases and environmental carbohydrates.

Safety: This product is non-hazardous and has been purified from natural sources certified to

be free of all hazardous material including pathogenic biological agents.

For research use only. Not for human or drug use

Warranties and liabilities

Ludger warrants that the above product conforms to the attached analytical documents. Should the product

fail for reasons other than through misuse Ludger will, at its option, replace free of charge or refund the

purchase price. This warranty is exclusive and Ludger makes no other warrants, expressed or implied,

including any implied conditions or warranties of merchantability or fitness for any particular purpose.

Ludger shall not be liable for any incidental, consequential or contingent damages.

This product is intended for in vitro research only.

Document # 'CAB-GHP-Guide', revision 1.1

Certificate of Analysis

LudgerPureTM 2AB Labeled Glucose Homopolymer

Cat. # : CAB-GHP-30 Lot # : A3AO-01 Size : approx. 1 nmol of G1

Purity: > 95% pure as assessed by HPLC (see Fig 1).

0 10-200

500

1,000

1,500

2,000 LudgerSepN1-A51Q-01-CAB-GHP-30-A3AO-01 FluorescencemV

10.5

1

13.2

7

Figure 1 : LudgerS

No. A3AO-01).

HPLC Running C

Column: LudgerSep N

Solvent A: 100 % aceton

Gradient: 0-7

75-

80-

83-

85-

Detector: Jasco FP920

Sample Dilution

Redissolve CAB-GHP-

GHP with 60 µl aceton

G1

20

16.7

3

20.8

1

5

epN1

onditio

1 Amide

itrile

5 min -

80 min -

83 min 1

85 min -

115 min

30 in 300

itrile and

G

30 40

25.2

1

29.6

7

33.9

7

38.0

7

HPLC Profile of

ns

(Cat. no. LS

Solvent B: 2

35-65 % B

65-100 % B

00 % B

100-35 % B

35 % B

Excitation wavelengt

µl analytical grade w

20 µl water.

G10

50 60 70 80 90 100 110 116

min

41.8

5

45.3

2

48.4

9

51.3

9

54.1

2

56.5

858

.82

60.9

362

.88

64.6

766

.38

67.9

169

.39

70.7

371

.95

73.1

474

.22

2AB Labeled Glucose Homopolymer (Cat. # : CAB-GHP-30, Lot

-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

50 mM ammonium formate pH 4.4

h: 360 nm Emission wavelength: 425 nm

ater. Approximately 10 µl of reconstituted sample is used per hplc run. Inject 10 µl