2018 9th International Conference on Food Engineering and ...icfeb.org/ICFEB2018-program.pdf · Ninik Rustanti 21 C3004: Prohibited Coloring Agent in Dominating Hazardous Street Food

2018 HKCBEES OKINAWA CONFERENCE

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2018 9th International Conference on Food

Engineering and Biotechnology

(ICFEB 2018)

March 28-30, 2018

University of the Ryukyus, Japan

Sponsored and Published by

Indexed by

www.cbees.org

http://www.cbees.org/


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Table of Contents

2018 HKCBEES Okinawa Conference Introductions

6

Presentation Instructions 7

Keynote Speaker Introductions 8

Schedule for Conference 14

Session 1

C0002: Technological Development of Probiotic Supplement for Zootechnical

Improvement of Broilers

Magali T. Uono, Sibylle S. Hacker, Catarina V. Manfrinato, Mariana M. Matsuo,

Svetoslav D. Todorov and Cristina S. B. Bogsan

16

B4003: Design and Tests of Hemp Harvester

Huang Jicheng and Shen Cheng

16

B0013: Establishment of New Cell Lines from Pupal Ovaries of Spodoptera exigua with

Differential Susceptibility to Homologous Nucleopolyhedrovirus

Sudawan Chaeychomsri, Win Chaeychomsri, Jindawan Siruntawineti, Motoko Ikeda

and Michihiro Kobayashi

17

B0015: Effect of Colistin and Liquid Methionine with Capsaicin Supplementation in

Diets on Growth Performance and Intestinal Morphology of Nursery Pigs

Thitima Jarupan, Chaowit Rakangthong, Chaiyapoom Bunchasak, Theerawit

Poeikhampha and Pudcharaporn Kromkhun

17

B4002: Design, Analysis and Test on Cutting Test Bench of Ramie Stalk

Shen Cheng

18

B0014: Effect of Colistin and Liquid Methionine with Capsaicin Supplementation in

Diets on Blood Chemical and Intestinal Bacteria of Nursery Pigs

Theerawit Poeikhampha, Chaowit Rakangthong, Chaiyapoom Bunchasak, Thitima

Jarupan and Wiriya Loongyai

18

B0016: Effects of Liquid Methionine and Capsaicin Supplementation in Diets on

Growth and Intestinal Morphology of Broilers

Wararat Arparjirasakul, Chaiyapoom Bunchasak, Chaowit Rakangthong and

Theerawit Poeikhampha

19

Session 2


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B0004: Non-destructive Colorimetric Sensor Applications for Agro-food Products

Yong Hoon Kim, Chun Wan Park, Jin Se Kim, Dong Soo Choi, Su Jang Lee and Seok

Ho Park

20

C0003: Development of a Desktop Food Printer for Dough Extrusion

Jie Sun, Geok Soon Hong, Sanjairaj Vijayavenkataraman and Johandy Tantra

20

C4001: Chromatic and Chemical Properties of Mao Berry as a Key Parameter for

Industry

Wanphen Jitjaroen, Laddawan Papin, and Lachinee Panjai

20

C3003: Effect of Processing Methods on Glycemic Index of Chocolate Crackers Made

with Modified Kepok Banana [Mussa paradisiaca L.] Flour

Diana Nur Afifah, Anindya Selviana Putrianti, Andrea Putri Sekar Tunjung and

Ninik Rustanti

21

C3004: Prohibited Coloring Agent in Dominating Hazardous Street Food around

Elementary School in Semarang-Indonesia

Gemala Anjani, Ninik Rustanti, Hartanti Sandi Wijayanti, Trisna Suryaningrum and

Diana Nur Afifah

21

B0002: Homogenization and Microwave Irradiation Enhancements of Betacyanin

Extraction from Pitahaya Peel and Its Stability

Songchai Wiriyaumpaiwong and Arpaporn Prapasanobon

22

C0014: Isolation and Characterization of Biosurfactant Produced by Lactic Acid

Bacteria from Indigenous Thai Fermented Foods

Fatin Ruangprachaya and Pumnat Chuenchomrat

22

Session 3

C0009: Production of Glucosyl Rebaudioside A via Enzymatic Bioconversion and Their

Characteristics as a Sweetener

So-Hyeon Lee, Jin-A Ko, So-Jin Yang, So-Yeon Kim, Hae-Soo Kim, Min-Ho Jo,

Joong-Su Kim, Young-Jung Wee, Jeong-Yong Cho, Su-Jeong Choi, Young-Min Kim

23

C0004: Rapid Detection of Gene HLA-A3101 on Membrane Based Lateral-Flow Strips

Jui-Chuang Wu and Yu-Shiun Huang

23

C0010: IGF2 Induces CD133 Expression in Esophageal Cancer Cells to Promote Cancer

Stemness

Bin Li

24

C0013: Study of Regulation Mechanism of Autophagy by Periodontal Pathogen in

Diabetes Model

Mi Hee Park, Hee Sam Na, Yuri Song, Seyeon Kim, Hyun Ah Lee, Jin Chung

25

C3005: Relationship of Waist Circumference and Percent Body Fat With Adolescent

Leptin-Adiponectin Levels Indonesia

Nuryanto, Subarjati A and Sulchan M

25

B0007: Imperatorin Suppresses Esophageal Cancer Metastasis through Inhibition of

TGFβ2/ERKsignaling Pathway

26


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Wen Wen Xu, Bin Li

C0017: Effects on Rats at Reflux Esophagitis Treated with Gardeniae Fructus 50%

EtOH Extract (GE)

Soo Hyun Kim, Seong-Soo Roh

26

Session 4

B4004: Phytoremediation of Heavy Metal Contaminated Soils with Maize of Different

Varieties

Kokyo Oh, Shinichi Yonemochi, Miwa Makoto, Tetsushi Yonekura, Yugo Isobe,

Hongyan Cheng, Yinghe Xie, Jianping Hong, Tiehua Cao

27

C0011: Biochemical Characteristics of Alkaliphilic Cyclodextran Glucanotransferase for

Cyclodextran Production

So-Jin YANG, Hae-Soo KIM, So-Hyeon LEE, So-Yeon KIM, Min-Ho JO, Su-Jeong

CHOI, Ju-Hui KIM and Young-Min KIM

28

C3002: Encapsulated Vitamin D3 in Liposome Coating by β-Lactoglobulin

Gemala Anjani, B.Panungga, D.N. Afifah, A.Pramono, M.Sulchan and A. Ohta

29

C1003:Niger Delta Remediation-to-Biofuel Solution: Hydrocarbon Pollution

Management to Wealth Creation

Ayodele A. Otaiku

29

C0008: Acid Hydrolysis Gives the High Concentration of Gallic Acid in Green Tea

Extract

Hae-Soo Kim, Gyeoung-Bin Park, So-Hyeon Lee, Min-Ho Jo, Su-Jeong Choi, Ju-Hui

Kim, Seung-Hee Nam, Jong-Bang Eun and Young-Min Kim

30

B0010: Simulation of Soil Failure around the Subsoiler Type Curved Leg using

Computational Fluid Dynamics

SAMART BUN-ART and KRIENGKRAI RAYANASUK

30

B0008: Data Engineering Analysis of Fruit Bug Characteristics for Pear Cultivation

Kazuya Miyakawa and Tatsuya Yamazaki

31

Poster Session

C3001: Preparation and Physicochemical Properties of the Isomaltooligosaccharides

Using Puffed-rice Flour

Bo-Ram Park, Han Suk Choi and Shin-Young Park

31

B0005: The current state of custom works in agriculture of the Republic of Korea

Jeongmin Lee, Yuyong Kim, Byounggap Kim

32

B0006: Case Study on a Rainwater Catchment Device for Water Harvesting in Upland

Farm in Long Slope Land

Youngjin Kim, Yonghun Choi, Minyoung Kim and Jonggil Jeon

33


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Listeners 34

One Day Visit 35

Conference Venue 36

Note 37

Feedback Information 40


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2018 HKCBEES Okinawa Conference

Introductions

Welcome to CBEES 2018 conference in Okinawa, Japan. The objective of the Okinawa conference is to provide a platform for researchers, engineers, academicians as well as industrial professionals from all over the world to present their research results and development activities in Food Engineering and Biotechnology.

2018 9th International Conference on Food Engineering and Biotechnology (ICFEB 2018)

Papers will be published in one of the following journals:

International Journal of Food Engineering (IJFE, ISSN: 2301-3664), which is

indexed by WorldCat, Google Scholar, Cross ref, ProQuest , CABI.

Journal of Advanced Agricultural Technologies (JOAAT,ISSN:2301-3737),

which is indexed by Engineering & Technology Digital Library, and indexed by

Ulrich's Periodicals Directory, Google Scholar, Engineering & Technology Digital

Library, Crossref and Electronic Journals Digital Library;

International Journal of Bioscience, Biochemistry and Bioinformatics, which is

indexed by Ulrich's Periodicals Directory, Google Scholar, Engineering &

Technology Digital Library, Crossref and Electronic Journals Digital Library, et al;

Conference website and email: http://www.icfeb.org/; [email protected]

mailto:[email protected]


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Presentation Instructions

Instructions for Oral Presentations

Devices Provided by the Conference Organizer:

Laptop Computer (MS Windows Operating System with MS PowerPoint and Adobe Acrobat

Reader)

Digital Projectors and Screen

Laser Sticks

Materials Provided by the Presenters:

PowerPoint or PDF Files (Files should be copied to the Conference laptop at the beginning of

each Session.)

Duration of each Presentation (Tentatively):

Keynote Speech: about 30 Minutes of Presentation and 5 Minutes of Question and Answer

Regular Oral Presentation: about 12 Minutes of Presentation and 3 Minutes of Question and

Answer

Instructions for Poster Presentation

Materials Provided by the Conference Organizer:

The place to put poster

Materials Provided by the Presenters:

Home-made Posters

Maximum poster size is A1

Load Capacity: Holds up to 0.5 kg

Best Presentation Award One Best Oral Presentation will be selected from each presentation session, and the

Certificate for Best Oral Presentation will be awarded at the end of each session on March 29,

2018.

Dress code Please wear formal clothes or national representative of clothing.


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Keynote Speaker Introductions

Keynote Speaker I

Prof. Kokyo Oh

Center for Environmental Science in Saitama, Japan

Prof. Kokyo Oh is a senior researcher in Center for Environmental Science in Saitama,

Japan. He graduated with his MSci degree and Ph.D. degree (soil science) in Chinese

Academy of Sciences, and was honored as a STA research fellow by Japan government from

1997 to 1999. The research areas include soil science, environmental conservation,

environmental chemistry, and atmospheric environment. His current research is mainly on soil

remediation, environmental agronomy, atmospheric PM2.5 and water environment

conservation. He has published more than 90 publications

Topic: “Development and Technology of Environmental Friendly Agriculture in Japan”

Abstract—Agriculture is the most dominant human influence on sustainability and

environmental quality of the earth. Japan is a mountainous country with high population

density and very limited flat land, which is quite unfavorable for its agricultural production

and environmental conservation. However, Japan has developed its own environmental

friendly agricultural way to conserve the multiple functions of agriculture, and to ensure the

agriculture maintains at high level in quality, productivity, safety and environmental

conservation. This presented study briefly introduced the policies, current situation,

development, and technologies of the environmental friendly agriculture in Japan. This

research provides important information to sustain agricultural productivity and enhance food

security and environmental quality.


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Keynote Speaker II

Prof. Yong-Suk Jang

Chonbuk National University, Republic of Korea

Prof. Yong-Suk Jang has completed his PhD from Northwestern University, Chicago, IL,

USA at 1993, and postdoctoral study at Cancer Research Institute of Seoul National

University Medical School, Seoul Korea. He is a professor and chairman in the Department of

Molecular Biology, College of Natural Science, Chonbuk National University, Republic of

Korea. He has published more than 150 papers in reputed journals including the Journal of

Immunology, Biomaterials, European Journal of Immunology, and Scientific Reports. He has

been elected as a President of the Korean Association of Immunologists for 2019. His current

research is mainly on mucosal immune regulation and mucosal vaccine development Topic: “Development of Oral Mucosal Vaccine Using Antigen-targeting Strategy to M

Cells in the Mucosal Immune Compartments”

Abstract—Mucosa-associated lymphoid tissue (MALT) is defined as solitary organized

mucosa-associated lymphoid follicles and is subdivided by anatomical regions. MALT is

characterized as lacking afferent lymphatics, therefore only taking up exogenous antigens

through its follicle-associated epithelium, which contains enterocytes, goblet cells, and

microfold (M) cells. Among the MALT, the gastrointestinal mucosa maintains a tolerogenic

microenvironment to protect the body from unwanted induction of the immune response to

continuously exposed commensal microorganisms and food antigens. Considering that 90%

of infections occur in mucosal areas, it is conceivable that using mucosal vaccination to

establish protective immunity in this frontline of pathogen infection could offer great

advantages in current vaccination strategy. However, the number of currently available oral

vaccines is very limited compared to the number of parenteral vaccines. This limited

availability of oral mucosal vaccines is closely related with the lack of an effective antigen

delivery system and a strong adjuvant to stimulate immunity due to the intrinsic nature of the

mucosal immune system, which has a low efficiency in antigen delivery into the inductive site

and a tendency to induce oral tolerance. We have concentrated our efforts to elaborate

efficient antigen delivery system to M cells to establish the strategy for effective oral mucosal

vaccine development. Various antigen-targeting strategy with adjuvant activity which includes

peptide ligands, adhesion molecule, and antimicrobial peptide against various viral antigens

will be discussed. (This study was supported by the research fund from Korea Research

Institute of Chemical Technology, CEVI-2016-3-1 and by the National Research Foundation

(NRF) funded by the Korean Ministry of Science, ICT, & Future Planning,

2014K1B1A1073861 & 2016R1A2B2010096.)


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Keynote Speaker III

Honorary Distinguished Prof. Jinchyau Peng

National Chung-Hsing University, Taiwan

Prof. Jinchyau Peng was born in Taiwan. His major at his BS degree (National Chung-Hsing

University at 1976) and MS degree (National Taiwan University at 1978) was in Agricultural

Machinery. He completed his PhD from University of Missouri-Columbia, MO, USA at 1991

and major in Food Engineering. Since 1980, he has been working in National Chung-Hsing

University as an Instructor, Associate Professor, Professor, and Distinguished Professor. Now

he is an Honorary Distinguished Professor. He had been as a chairman in the Bio-industrial

Mechatronics Engineering Department from 2002 to 2005. He had received the ―Youth

Medal‖ by the Government at 1985 and the ―Academic Achievement Award‖ by the Institute

of Chinese Agricultural Machinery at 2007. His research areas include the applications of

extrusion technology, food engineering and food machinery, the developments of agricultural

machinery. He has published more than 110 papers in different reputed journals and more than

130 meeting and conference reports.

Topic: “The Development and Experiments of a Dehumidified Dryer”

Abstract—Drying is an important operation in the food (or agricultural products) process.

The purposes of drying operation are to extend the products storage period, to prevent the

damage due to the microorganism, and to enhance the products quality. The drying methods

used will affect the products quality (aroma, color and flavor) and costs. The drying methods

such as sun drying, hot air drying, freeze drying, and dehumidified drying are often used

based on different situations. The sun drying is the most traditional method and it depends on

the weather conditions. The hot air drying method is the most common used among all the

drying methods because of its low costs and convenient operation. But, the aroma and flavor

is removed from the raw materials and therefore it reduced the products quality. The freeze

drying method is the most expensive method and it is applied to the high price products

drying.

The dehumidified dryer which consists of air inlet, heater, drying chamber, condenser, and

condensed water outlet, was designed and developed by Dr. Peng and Sung-Yeou Co., and is

extended and commercialized in Taiwan. The experiments using dehumidified dryer for

agricultural products such as Lotus nut, Lotus flower, Chinese Jujube, Orange Daylily,

Chinese yam, Roselle, Obtuse leaf etc. were conducted in order to investigate the drying

characteristics of all the products. All the experiments showed that the dehumidified drying

method is the most useful method among all the drying methods. The advantages of the

dehumidified drying method are as follows: (1) Shorten the drying time. (2) Maintain the

products aroma, flavor, color, and therefore enhance the products quality. (3) Lower the

drying costs. (4) Multiple usages of the condensed water.


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Keynote Speaker IV

Prof. Shigeru Kato

Seikei University, Japan

Prof. Shigeru Kato was born in Tokushima, Japan, 1947. His experences are as following:

March 1970, B.S. (Agricultural Chemistry), Faculty of Agriculture, Tokyo University of Agriculture

April 1971, Graduate School (Agricultural Chemistry course), Tokyo University of Agriculture (until

March 1976)

March 1976, Ph.D. (Agricultural Chemistry), Tokyo University of Agriculture

April 1976, Visiting Fellow, NIEHS (National Institute of Environmental Health Sciences), NIH (National

Institute of Health), US Government (until June 1979), North Carolina, USA

July 1979, Research Associate, NRI (NODAI Research Institute), Tokyo University of Agriculture (until

March 1982)

April 1982, Lecturer, NRI (NODAI Research Institute), Tokyo University of Agriculture (until March

1990)

April 1990, Associate Professor, NRI (NODAI Research Institute), Tokyo University of Agriculture (until

March 1995)

April 1995, Professor, NRI (NODAI Research Institute), Tokyo University of Agriculture (until April 1997)

May 1997, Visiting Scientist, RCAST (Research Center for Advanced Science and Technology), The

University of Tokyo (until April 1998)

July 1998, Invited Researcher, NEDO (New Energy and Industry Technology Development Organization),

Ministry of Economy, Trade and Industry (until March 2001)

April 2003, Invited lecturer and Research associate, Department of Applied Chemistry, Faculty of

Engineering, Seikei University (until March 2007)

April 2007, Professor (Special Invited Research Professor), Department of Materials and Life Science,

Faulty of Science and Technology, Seikei University Topic: “Rehabilitation of Abandoned Mangroves in Nakhon Si Thammarat, Thailand”

Abstract—Mangroves are a type of tropical forest, uniquely positioned at the dynamic

interface of land and sea. Mangroves distribute along coast and estuaries throughout the

tropics and subtropical regions of the world. Mangroves are one of the most important forests

as carbon sink for global warming mitigation ecosystem. Mangrove forests are of great

importance to coastal communities for providing as food and energy sources. Mangrove forest

provides numerous ecosystem services in a buffer zone between anthropogenically polluted

areas and the sea, including nutrient cycling, carbon sequestration from atmosphere, sediment

trapping, land protection from typhoons and tsunami, wood for fuel and lumber and habitat

for living organisms like crab, shell, shrimp and fish.

I would like to introduce about study on the carbon accumulation and food chain (web)

system in the rehabilitated mangrove sites of Nakhon Si Thammarat, southern Thailand. The

study sites, the abandoned shrimp ponds and the new mud flat areas, have been rehabilitating

since 1998 through mangrove planting. More than eight million mangrove seedlings were


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planted in more 1,300 ha. Carbon content in soil at rehabilitated mangrove forest was

gradually increased as planting age from the state of the abandoned shrimp ponds and also

new mud flat areas, respectively. Rehabilitated mangrove forests would be as a sink source

for atmospheric carbon dioxide through photosynthesis by mangrove trees and habitat for

rehabilitating and increasing rich biodiversity of the estuaries ecosystem.

The results were shown that the δ15

N content in the living organism is gradually increased

from small phytoplankton to large fishes through the food chain system. Conversely, there is

significant change of the δ13

C value through the food chain system. The data analysis reveals

that the carnivorous fishes involve in 22th to 23th step of the food chain system which starts

from mangrove leaves of the rehabilitated mangrove forest.


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Keynote Speaker IV

Prof. Nyuk Ling Chin

Departmen of Process and Food Engineering, Universiti Putra Malaysia

Prof. Nyuk Ling Chin obtained her Ph.D in Chemical Engineering from University of

Manchester Institute of Science and Technology (UMIST), United Kingdom at the age of 26.

She is now a Professor with the Department of Process and Food Engineering, Faculty of

Engineering, Universiti Putra Malaysia. She is a registered professional food engineer with

Board of Engineers Malaysia and a chartered chemical engineer with Institute of Chemical

Engineer, UK. She lectures on food engineering operations and systems. Her research is on

physical and engineering properties of food and natural products with aims of structuring food

tailored towards end-user functionality for improved texture, appearance, perception, shelf life

stability, consumer acceptability and healthiness. Her focus operations include mixing,

heating, baking, spray-drying, freeze-drying and extraction where physical properties

including rheological aspects are studied fundamentally. Her most recent projects are on food

authentication; in particular, honey and bird nest origin traceability. She has 125 journals

published. She receives recognitions from the many professional services rendered being an

invited foreign lecturer, an editor of Journal of Food and Bioproducts Processing and others,

an invited speaker in local and international conferences and seminars, a panel judge for

research competitions, a panel reviewer for research grant proposals, a panel examiner for

curriculum and programme reviews, and a visiting professor to other institutions.

Topic: “Chemical and Genetic Markers for Identification of Honey Origin from Its Bee

Speciation”

Abstract—The need for accurate and reliable methods for identification of honey origin is

important for reducing honey fraud. This study has identified suitable chemical and genetic

markers to determine the origin of honey from its bee source of Apis honey bees or Trigona

stingless bee. In the chemical analysis, moisture, fructose, glucose, sucrose, free acidity, and

colour intensity were chemical markers identified for differentiating honey by its bee origin.

The use of principal component analysis (PCA) and hierarchical cluster analysis (HCA) on

honey composition have successfully classified honey into groups of Apis and Trigona. In the

DNA-based method, mitochondrial cytochrome c oxidase subunit I (COI) gene was used as a

genetic marker to identify honey origin by its bee species accurately from the clear groupings

and distinct clusters in phylogenetic trees. The genetic marker of COI gene is accurate and

reliable for this identification as it has direct matching to its reference bee species.

Incorporating both chemical and genetic markers affirm the identity of honey.


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Schedule for Conference

Day 1

March 28, 2018 (Wednesday) 10:00~17:00

Venue: Engineering Building 1-221

Arrival Registration and Conference Materials Collection

Day 2

March 29, 2018 (Thursday)


Arrival Registration, Keynote Speech, and Conference Presentation

Morning Conference （Venue: Engineering Building 1-222）

Opening Remarks 8:55~9:05

Prof. Dongshik KANG, University of the Ryukyus, Japan

Keynote Speech I 9:05~9:40

Topic: ―Development and Technology of Environmental Friendly

Agriculture in Japan‖

(Prof. Kokyo Oh, Center for Environmental Science in Saitama,

Japan)

Keynote Speech II 9:40~10:15

Topic: ―Development of Oral Mucosal Vaccine Using

Antigen-targeting Strategy to M Cells in the Mucosal Immune

Compartments‖

(Prof. Yong-Suk Jang, Chonbuk National University, Republic of

Korea)

Keynote Speech III 10:15~10:50

Topic: ―The Development and Experiments of a Dehumidified

Dryer‖

(Prof. Jinchyau Peng, National Chung-Hsing University, Taiwan)

Coffee Break and Group Photo Taking 10:50~11:20

Keynote Speech IV 11:20~11:55

Topic: ―Rehabilitation of Abandoned Mangroves in Nakhon Si

Thammarat, Thailand‖

(Prof. Shigeru Kato, Seikei University, Japan)


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Keynote Speech V 11:55~12:30

Topic: ―Chemical and Genetic Markers for Identification of

Honey Origin from Its Bee Speciation‖

(Prof. Nyuk Ling Chin, Departmen of Process and Food

Engineering, Universiti Putra Malaysia)

Lunch 12:30~14:00

Afternoon Conference

Session 1 14:00~15:45

Venue: Engineering Building

1-222

7 presentations-Topic: ―Agricultural

Science and Technology‖

Session 2 14:00~15:45


7 presentations-Topic: ―Food Science

and Engineering‖

Coffee Break 15:45~16:00

Session 3 16:00~17:45

Venue: Engineering Building

1-222

7 presentations-Topic: ―Bioscience

and Biotechnology‖

Session 4 16:00~17:45


7 presentations-Topic: ―Environmental

and Chemical Engineering‖

Poster Session 8:55~17:45


3 posters

Dinner 18:00

Day 3 One Day Visit

Tips: Please arrive at the conference room 10 minutes before the session begins to upload

PPT into the laptop.

Note: (1) The registration can also be done at any time during the conference.

(2) The organizer doesn’t provide accommodation, and we suggest you make an early reservation.

(3) One Best Oral Presentation will be selected from each oral presentation session, and the

Certificate for Best Oral Presentation will be awarded at the end of each session on March 29, 2018.


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Session 1 Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,

we strongly suggest that you attend the whole session.

Afternoon, March 29, 2018 (Thursday)

Time: 14:00~15:45


7 presentations- Topic: “ Agricultural Science and Technology”

Session Chair: Prof. Tatsuya Yamazaki

C0002 (Session 1) Presentation 1 (14:00~14:15)

Technological Development of Probiotic Supplement for Zootechnical Improvement of

Broilers

Magali T. Uono, Sibylle S. Hacker, Catarina V. Manfrinato, Mariana M. Matsuo, Svetoslav

D. Todorov and Cristina S. B. Bogsan

Department of Biochemical-Pharmaceutical Technology, University of Sao Paulo, Brazil

Abstract—The increasing of the demand for functional foods, which include those

supplemented or fermented by probiotic microorganisms, resulted in the advancement of

research and development of new strains. The probiotic microorganisms Bacillus spp. are

attractive for the inherent stability due to their characteristic of spore-forming bacteria.

Spores allow prolonged shelf life and increase the ability to survive gastric barriers, which

turn out to be an advantage over lactobacilli. Two bacteria were supplied by VitaBridge

Company in order to identify which strain would have the best technological performance for

the development of a novel functional food with high performance in the zootechnical

improvement of broilers. The characterization was performed according to the protocol

which included phenotypic and genotypic tests for determining the probiotic potential of the

strains. The results of the tests revealed that BVB1 possesses the best technological

characteristics and can be used with efficiency in the improvement of the zootechnical

performance of broilers.

B4003 (Session 1) Presentation 2 (14:15~14:30)

Design and Tests of Hemp Harvester

Huang Jicheng, Shen Cheng

Nanjing Research Institute for Agricultural Mechanization, Ministry of Agriculture of the

P.R.C., Nanjing 210014, China

Abstract—For the current situation that mechanization of hemp is at a low level and its

relevant harvest equipment is in shortage, a hemp harvester suitable for field work in main


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producing areas of hemp has been designed based on physical parameters obtained from

statistical analysis of main hemp varieties. This type of harvester is equipped with a dedicated

power chassis, and the main body of harvester bench includes stalk lifter, cutter, three groups

of chain transverse conveyers, longitudinal force conveyer and collecting device, etc. The

motive power is driven by hydraulic system, by which cutting, conveying and charging of

industry hemp can be completed at a time. Field test showed that working parts of the

harvester were in stable operation status with a good harvesting effect. The rate of miss

cutting was 5.8%~10.8%, qualification rate of cutting height was 79%~92%, conveying

success rate was 81%~91%, and production rate was 0.15~0.22 hm2·h

-1, which basically met

mechanized harvest requirement of hemp. This study provides a reference to realize

mechanized harvest of hemp.


Establishment of New Cell Lines from Pupal Ovaries of Spodoptera exigua with Differential

Susceptibility to Homologous Nucleopolyhedrovirus

Sudawan Chaeychomsri, Win Chaeychomsri, Jindawan Siruntawineti, Motoko Ikeda and

Michihiro Kobayashi

Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand

Abstract—Four new cell lines from pupal ovaries of Spodoptera exigua were established.

These cell lines consisted of round and spindle-shaped cells. The cell lines were examined for

their population doubling time and susceptibility to the homologous virus, S. exigua multiple

nucleopolyhedrovirus (SeMNPV). One cell line, designated KU-SEPO-2 (SEPO2), had

significantly shorter population doubling time than that of other cell lines. The results and

observations strongly indicated that the susceptibility of the cell lines to SeMNPV varied from

fully susceptible to resistant. The SEPO2 cell line was highly susceptible to SeMNPV. By 5

days postinfection (pi), 100% of the cells contained occlusion bodies (OBs). Ultrastructural

studies showed that SeMNPV replication and OB formation in the infected SEPO2 cells were

similar to that normally observed in vivo. This pupal ovarian cell line derived from S. exigua

had distinct characteristics in growth and susceptibility to the homologous virus. The

availability of this cell line will provide a useful tool for the basic research on NPVs and for

future studies of virus-host cell interactions.


Effect of Colistin and Liquid Methionine with Capsaicin Supplementation in Diets on Growth

Performance and Intestinal Morphology of Nursery Pigs

Thitima Jarupan, Chaowit Rakangthong, Chaiyapoom Bunchasak, Theerawit Poeikhampha

and Pudcharaporn Kromkhun

Kasetsart University, Bangkok, Thailand

Abstract—This study was conducted to evaluate comparative effect of Colistin and Liquid

methionine (LMA) with Capsaicin supplementation in diets on growth performances and

intestinal morphology of nursery pigs. Seventy-two crossbred pigs (Duroc x Large White x

Landrace; initial weight 6.76±0.22 kg) were divided into three groups with six replications of

eighteen piglets each. There were three dietary treatment: 1) basal diet (control) 2) basal diet


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with anitibiotic (Colistin 40 ppm of diet) and 3) basal diet with 0.2% LMA with Capsaicin.

The result show that the supplemental of 0.2% LMA with Capsaicin significantly improve

feed conversion ratio of pigs during pre-starter period (p<0.01). Adding Colistin and LMA

with Capsaicin to diets can increase villous in the duodenum and ileum (p<0.01) and the

villous height:crypt depth ratio in duodenum and jejunum. The supplement of LMA with

Capsaicin significantly increase villous height and may lead to absorb nutrient for growth in

pigs. In conclusion, this study indicates that adding LMA with capsaicin in diets can reduce

antibiotic in diets of nursery pigs.


Design, Analysis and Test on Cutting Test Bench of Ramie Stalk

Shen Cheng

Nanjing Research Institute for Agricultural Mechanization, Ministry of Agriculture of the

P.R.C., Nanjing 210014, China

Abstract—A ramie stalk cutting test bench was proposed in the paper to study the cutting

performance of ramie stalk so as to offer theoretical basis and technical support for the

research on ramie harvester. The cutting test bench suggested could adjust the cutting average

speed and feed conveying average speed of ramie stalk from 0 to 2 m/s. The cutting

transmission mechanism of the test bench was designed as a space crank-rocker linkage

mechanism. The analysis on kinematics of transmission mechanism and its simulation

indicated that the kinetic characteristics of mechanism were similar to the characteristics of

sine. Additionally, no-load test and ramie stalk cutting test were conducted for the cutting test

bench. According to the test results, peak driving force of the cutter and cutting energy in the

single cutting cycle increased with the rise of cutting velocity under unloaded state.

Otherwise, peak cutting force of the cutter and cutting energy in the single cutting cycle

reduced with the rise of cutting velocity during ramie stalk cutting test. The cutting test data

have some reference significance for the follow-up study on harvesters.


Effect of Colistin and Liquid Methionine with Capsaicin Supplementation in Diets on Blood

Chemical and Intestinal Bacteria of Nursery Pigs

Theerawit Poeikhampha, Chaowit Rakangthong, Chaiyapoom Bunchasak, Thitima Jarupan

and Wiriya Loongyai


Abstract—This study was conducted to determine comparative effect of supplemented

Colistin and Liquid Methionine (LMA) with Capsaicin in diets on blood chemical and

bacteria population in caecum of nursery pigs. Seventy-two crossbred pigs (Duroc x Large

White x Landrace; initial weight 6.76±0.22 kg) were divided into three groups with six

replications of eighteen piglets each. There were three dietary treatment: 1) basal diet

(control) 2) basal diet with anitibiotic (Colistin 40 ppm of diet) and 3) basal diet with 0.2%

LMA with Capsaicin. The results indicated that supplemental of Colistin in diets significantly

decreased lymphocyte (p<0.05), increased of neutrophil and neutrophil:lymphocyte ratio

(p<0.05) compared with control group. In addition, supplementing both Colistin and LMA


- 19 -

with Capsaicin can increased population of Domain bacteria and Lactobacillus sp. in caecum

of nursery pigs. In conclusion, this study indicates that supplementing of LMA with capsaicin

in diets can reduce antibiotic in diets of nursery pigs.


Effects of Liquid Methionine and Capsaicin Supplementation in Diets on Growth and

Intestinal Morphology of Broilers

Wararat Arparjirasakul, Chaiyapoom Bunchasak, Chaowit Rakangthong and Theerawit

Poeikhampha


Abstract—The experiment was conducted to investigate the effects of Liquid Methionine

(LMA) and capsaicin in broiler. In the first study, an in vitro study was conducted

investigating the antimicrobial efficacy of the combination between LMA and capsaicin

against clinical isolates and reference laboratory strains of Escherichia coli ATCC25922. The

minimum inhibitory concentration for Escherichia coli ATCC25922 was 0.125 %. The second

study, a total of 192 commercial male broiler chicks was used. Birds were raised for 38 days

in high ambient temperature and relative humidity in an open house. The chicks were divided

into 2 groups in 6 replications with 16 chicks each in a completely randomized design for

study the effects of supplementation of LMA 0.05% and capsaicin 2.5 ppm supplementation

in diets on growth and intestinal morphology of broiler reared in open housed condition. The

results indicated that broilers fed with LMA combination capsaicin, had higher final average

daily gain (g) (P<0.05). However, the dietary treatments not influenced the feed intake, body

weight and intestinal morphology (P>0.05). Therefore, the concluded that supplementation

with LMA and capsaicin lead to improve the growth performance in live body weight of

broiler reared in open housed condition.

Session 2

Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,



Time: 14:00~15:45


7 presentations- Topic: “Food Science and Engineering”

Session Chair: Prof. Nyuk Ling Chin


- 20 -


Non-destructive Colorimetric Sensor Applications for Agro-food Products

Yong Hoon Kim, Chun Wan Park, Jin Se Kim, Dong Soo Choi, Su Jang Lee and Seok Ho

Park

National Institute of Agricultural Sciences, Rural Development Administration, 310

Nongsaengmyeng-ro, Wansan-gu, Jeonju, Korea

Abstract—From harvest immediately after to consumption, postharvest technology based on

storage and distribution is an effective method to minimize a property loss and to sustain the

high quality of safe agricultural products. Especially, a naked-eye detective colorimetric

sensor could communicate with consumers through colors which include the information of

agricultural products, such as quality and ripeness. In addition, this sensor offers the

time-saving convenience for the final purchasing decision as well as can be used as a

marketing tool to elevate the value of product. Herein, in order to achieve the advancement in

postharvest technology, along with the current research trend, various research fields were

integrated in the chemosensor fabrication. Monitoring the quality of agricultural products with

non-destructive methods is employed by on-packaging smart sensors which are sensitive to

fruit aroma and temperature. Fruit aroma sensitive colorimetric chemosensor utilizing pH dye

molecules is designed for the determination of apple ripeness. A temperature-response

colorimetric sensor is fabricated by thermochroic materials base on the electron transfer

between acceptor and donor. Non-destructive sensor applications by chemical approaches will

be presented.

This work was conducted with the support of the ―Cooperative Research Program for

Agriculture Science & Technology Development (Project No.: PJ01247301)‖ Rural

Development Administration, Republic of Korea. C0003 (Session 2) Presentation 2 (14:15~14:30)

Development of a Desktop Food Printer for Dough Extrusion

Jie Sun, Geok Soon Hong, Sanjairaj Vijayavenkataraman and Johandy Tantra

Xi'an Jiaotong-Liverpool University, China

Abstract—Food printing is increasingly being explored for mass customization of food. This

study reports the development of a desktop 3D printer for printing cookie dough. Specifically,

the effect of different extruder profiles on the properties of the extruded dough, are

experimentally evaluated to obtain an optimal design which consists of a screw, connection

profile between the cartridge and the syringe, and nozzles. Three different nozzle designs,

namely, conic, fat, and skinny nozzle profiles are tested. The conic nozzle design gives a good

balance between the speed of extrusion and extrusion consistency, compared with the other

designs. It is further modified to add a straight tube-like section to improve the extrusion

consistency. This study demonstrates the importance of dough extruder design in food

printing.


Chromatic and Chemical Properties of Mao Berry as a Key Parameter for Industry

Wanphen Jitjaroen, Laddawan Papin, and Lachinee Panjai


- 21 -

Department of Agro-industry, Rajamangala University of Technology Lanna Lampang,

Thailand

Abstract—The main physico-chemical composition of four mao cultivars (cv.) (Antidesma

thwaitesanum Müell.; Fah-pra-tann, Sann-home, Kham-ta, Heuy-bang) were investigated. The

cv. Fah-pra-tann and Kham-ta had the highest sugar content (193.58, and 190.90 g/L), with

the lowest acidity (6.55, and 6.76 g/L). The sugar acid ratio represent the balance taste

ranging from 13.72 to 30.02. The cv. Fah-pra-tann and Kham-ta were in appropriate range of

sugar x pH2 (238, and 226). The main acid was citric (5.02-10.98 g/L), and tartaric (3.36-5.51

g/L) acids. They were rich in phenolic (349.18-845.97 µgGAE/ml), anthocyanin

(583.07-2361.42 mg/l), and antioxidant activity (290.71-1213.80 µgQEAC/ml), being

inversely proportional to colour intensity (6.16-8.39), and were highest in the cv. Kham-ta.

They had similar contribution to the colouration between yellow and red pigments, and

correlated with the results for the tint and anthocyanin of the juices. It indicates that mao had

desirable characteristics to produce healthy food and interest products.


Effect of Processing Methods on Glycemic Index of Chocolate Crackers Made with Modified

Kepok Banana [Mussa paradisiaca L.] Flour

Diana Nur Afifah, Anindya Selviana Putrianti, Andrea Putri Sekar Tunjung and Ninik

Rustanti

Department of Nutrition Science, Faculty of Medicine, Diponegoro University, Indonesia

Abstract—Type 2 diabetes mellitus can be controlled or prevented by consuming foods which

had a low glycemic index (GI). Modified kepok banana (Mussa paradisiaca L.) flour was

made with an autoclaving-cooling method and spontaneous fermentation to produce low

glycemic index chocolate crackers as a snack for type 2 diabetes patients. This study analyzed

the organoleptic properties, the content of resistant starch and in vitro starch digestibility, the

glycemic index [GI], and glycemic load [GL] of chocolate crackers made with modified

kepok banana flour. The inclusion of modified kepok banana flour produced significant

differences in color, texture and taste in the crackers, which also had a low GI and GL. In

addition, the resistant starch content was unaffected by the production method, while the in

vitro starch and digestibility were affected by the amount of kepok banana flour used.


Prohibited Coloring Agent in Dominating Hazardous Street Food around Elementary School

in Semarang-Indonesia

Gemala Anjani, Ninik Rustanti, Hartanti Sandi Wijayanti, Trisna Suryaningrum and Diana

Nur Afifah


Abstract—Snack foods comprise ready-to-eat foods and beverages that are sold by traders.

Street foods in Indonesia are often misused by adding hazardous substances those are

prohibited or safe limits. The aim of this study was to un-derstand the street food quality and

safety in elementary schools in Semarang. This was an observational study con-ducted by a


- 22 -

research technique of simple random sampling. The target of the study was snack foods sold

around 32 elementary schools in the city of Semarang. The hazardous substances present in

the food were qualitatively analyzed by an easy method using easy testing kits. There were

four schools with no exposure to hazardous substances. In addi-tion, rhodamine B is the

most often substances added to snack foods primarily sauce, seasoning powder, and jam. The

percentage of street foods containing preservatives (formalin and borax), coloring agents

(rhodamine B and metanil yellow), and sweeteners (cyclamate and saccharin) is still high.

Therefore, It is necessary to control the sales of street foods, especially those containing

harmful food addi-tives, to prevent food adulteration.


Homogenization and Microwave Irradiation Enhancements of Betacyanin Extraction from

Pitahaya Peel and Its Stability

Songchai Wiriyaumpaiwong and Arpaporn Prapasanobon

Mahasarakham University, Maha Sarakham, Thailand

Abstract—The aims of this research are to enhance extraction of betacyanin from pitahaya

(dragon fruit - Hylocereus undatus) peel by using homogenization and microwave techniques

and assessed pigment stability under the different pH and heat treatments. We assessed

enhanced extraction with homogenization in distilled water at 2500 rpm at times of up to 20

minutes and 100W microwave power with times up to 30 seconds. After extraction, the

stability test under the different pH of 3-7 and heat treatment levels (without heat treatment,

pasteurization and sterilization) was also investigated. The homogenized extract after 20

minutes and microwave powered extract for 30 seconds led to the highest red color (measured

by a* value) and betacyanin content when compared with each other processing time. The

extracted pigment without heat treatment had higher stability than pasteurized and sterilized

extract in the pH 4-6 range.


Isolation and Characterization of Biosurfactant Produced by Lactic Acid Bacteria from

Indigenous Thai Fermented Foods

Fatin Ruangprachaya and Pumnat Chuenchomrat

Department of Food Science and Technology, Faculty of Science and Technology, Thammasat

University (Rangsit Center), Thailand

Abstract—Biosurfactant-producing lactic acid bacteria were isolated from the indigenous

Thai fermented foods. MRS-oil agar and surface tension measuring methods were used as

primary and secondary screening, respectively. There were 78 LAB isolates from 24 samples

of the indigenous Thai fermented foods showed halo zone on MRS-oil agar. PS001 isolate

derived from pickled fish (Pla Som) represented the lowest surface tension with 54.33 mN/m

of PBS supernatant. PS001 produced cell-bound biosurfactant as growth associated product.

For biosurfactant extraction, PBS supernatant was acid precipitated and followed by

extraction with a mixture of chloroform:methanol (2:1). Its chemical structure of purified

biosurfactant from PS001 was identified by TLC and FTIR spectroscopy as glycoprotein.


- 23 -

Session 3




Time: 16:00~17:45


7 presentations- Topic: “ Bioscience and Biotechnology”

Session Chair: Prof. Yong-Suk Jang


Production of Glucosyl Rebaudioside A via Enzymatic Bioconversion and Their

Characteristics as a Sweetener

So-Hyeon Lee, Jin-A Ko, So-Jin Yang, So-Yeon Kim, Hae-Soo Kim, Min-Ho Jo, Joong-Su

Kim, Young-Jung Wee, Jeong-Yong Cho, Su-Jeong Choi, Young-Min Kim

Department of Food Science & Technology and BK21 Plus Program, Chonnam National

University, Republic of Korea

Abstract—Rebaudioside A, derived from the leaf of Steiva rebaudiana Bertoni, has been used

as natural sweeteners and it is known to have high intensity sweetness and quality of taste.

However, its low solubility in water limits application in food and pharmaceutical products. In

this study, glucosyl rebaudioside A (GR) was synthesized through dextransucrase from

Leuconostoc lactis (LlDexT). GR was produced with 86% yield by LlDexT at 30°C and it

was isolated via Diaion HP-20 and C18 column chromatography with a step-wise ethanol

gradient. Finally, isolated GR has the molecular mass of 1129. Isolated GR showed the

following properties: First, GR improved the water solubility in aqueous solution at 25°C for

1 month. Second, GR showed high stability over 80% at broad pH range (2–10) and at

90–120°C for 2 h. Third, GR was also observed stable in commercially available soft drinks.

These results suggest that newly synthesized GR could serve as a high purity and sweetener in

soft drinks. C0004 (Session 3) Presentation 2 (16:15~16:30)

Rapid Detection of Gene HLA-A3101 on Membrane Based Lateral-Flow Strips

Jui-Chuang Wu and Yu-Shiun Huang

Department of Chemical Engineering, Chung Yuan Christian University, Taiwan

Abstract—Gene Human leukocyte antigen (HLA)-A3101 is known responsible of a medicine


- 24 -

allergy which may cause a fatal outcome. General clinics need a quick test to determine if a

patient is engaged with the related gene defect and avoid the medical prescription from

suspicious drugs. In this study, Gene HLA-A3101 is detected on membrane-based

lateral-flow (MBFL) strips, incorporated with an immune reaction and color readouts

developed by gold nanoparticles. The HLA-A-3101 DNA modified with biotin and

digoxigenin labels was amplified by the Polymerase Chain Reaction (PCR) and then detected

on the MBFL strips. The optimal condition of antibody concentration was at least 10µg/ml, to

avoid the antibody-gold complex from aggregation. The signal can be observed as low as 0.49

ng of the PCR product by naked eyes. The specificity of PCR primers was also investigated

their annealing validity. The detection specificity from other species genes, including a plant,

a virus, and a human gene, were also verified. The coefficient of variation values of the intra

assay, which tests the same sample in the same day, and the inter assay, which tests three

samples in three consecutive days, are in satisfactory ranges of 4.6% and 6.5%, respectively.


IGF2 Induces CD133 Expression in Esophageal Cancer Cells to Promote Cancer Stemness

Bin Li

College of Life Science and Technology, Jinan University, China

Abstract—Failure to eradicate cancer stem cells (CSC) during primary therapy may lead to

recurrence. We recently reported that CD133 is a functional biomarker for CSCs in

esophageal squamous cell carcinoma (ESCC), but the molecular pathways critical for survival

and maintenance of CD133-positive CSCs, which may be exploited in therapeutic targeting of

CSCs, are largely unknown. Here, we revealed that knockdown of IGF2 in ESCC cells

significantly suppressed CSC phenotypes including tumor initiation, sphere formation, and

chemoresistance. Conversely, IGF2 promoted these properties via activation of the PI3K/AKT

signalling pathway, and blockade of PI3K/AKT with inhibitors markedly inhibited the

abilities of CD133-positive ESCC cells to self-renew, resist chemotherapeutic drugs, and form

tumors. Further functional analysis identified miR-377as a downstream regulator of

PI3K/AKT signaling, and the results showed that miR-377 mediated the effects of IGF2 on

CD133 expression and CSC properties. By analysing a tissue microarray, we found that the

immunohistochemical expressions of IGF2 and CD133 were positively correlated with each

other in primary ESCC, and that concurrent elevation of IGF2 and CD133 levels was

significantly associated with poor survival outcomes in ESCC patients. Furthermore, our in

vivo experiments demonstrated that blockade of IGF2 with neutralizing antibody enhanced

the sensitivity of tumor xenografts to 5-FU treatmentin nude mice. This study underpins the

importance of the IGF2-PI3K/AKT-miR-377-CD133 signaling axis in the maintenance of

cancer stemness and in the development of novel therapeutic strategy for treatment of

esophageal cancer.

[This work was supported by the National Natural Science Foundation of China (81773085),

Guangdong Natural Science Research Grant (2016A030313838), and Guangzhou Science and

Technology Project (201707010260)].


Study of Regulation Mechanism of Autophagy by Periodontal Pathogen in Diabetes Model


- 25 -

Mi Hee Park, Hee Sam Na, Yuri Song, Seyeon Kim, Hyun Ah Lee, Jin Chung

Department of Oral Microbiology, School of Dentistry, Pusan National University, Korea

Abstract—Background and Objective: Diabetes is the metaboilic syndrome and its incidence

increases with age which is the important factor in the pathogenesis of diabetes. Furthermore,

periodontal pathogen-induced periodontal disease is well known as age-related inflammatory

disease. Although studies about relationship between periodontal disease are growing, there

no reports about effect of autophagy by periodontal pathogen on periodontal disease and

diabetes. Therefore, in this study, the regulatory mechanism for autophagy induction and

periodontal disease/diabetes/autophagy network induced by peridontal pathogen would be

investigated to clarify the relationship between periodontal disease and diabetes pathogenesis

and suggest the molecular targets for the effective control of diabetes

Methods: The differentiated THP-1 macrophages were stimulated by P. gingivalis xylitol in

low and high glucose conditions. The effect of glucose on cell viability was measured by

LDH assay. The production of IL-1β and TNFα was detected by ELISA. The expression or

activation of autophagy markers was Immunoblot analysis and confocal microscopy.

Results: Cell viability was not affect by glucose concentration in the differentiated THP-1

macrophages and HepG2 cells. The expression of autophagy marker was reduced in high

glucose condition, but the production of IL-1β and TNFα was increased by high glucose

condition. High glucose condition decreased P. gingivalis-induced autophagy and

inflammation in the differentiated THP-1 macrophages and HepG2 cells.


Relationship of Waist Circumference and Percent Body Fat With Adolescent

Leptin-Adiponectin Levels Indonesia

Nuryanto, Subarjati A and Sulchan M

Department of Nutrition, Faculty of Medicine, Diponegoro University, Indonesia

Abstract—Background: Levels of leptin and adiponectin are closely related to waist

circumference and percent of body fat. Changes in second marker levels on insulin sensitivity.

The aim of this study was to observe the association of the waist circumference and percent

body fat with the levels of leptin and adiponectin.

Method: A study using crossectional design with a total of 72 adolescents. Data on

circumference is a waist circumference which is the measurement of the waist circumference

of the subject measured using a tape meter with accuracy of 1 mm index. Percent body fat is

the difference between body weight and lean body mass as measured by using Bioelectric

Impedance Analyzer (BIA). Data on leptin and adiponectin seen in the levels of blood serum

taken using ELISA method. Relationship analysis of Waist Circumference and Percent body

fat with leptin level using Product Moment Correlation test (Pearson) and Rank Spearman.

Result: The mean waist circumference of sample is 81,75 ± 13,34 cm with mean body fat

percentage 32,26 ± 8,51%. The mean leptin content of 3.06 ± 0.51. The mean adiponectin

level was 6.29 ± 2.46. The test results showed no relationship between waist circumference

and leptin levels (r: 0.204; p: 0.087), but there was a negative relationship between waist

circumference and adiponectin levels (r: -0.539; p: 0,000). There is a positive relationship

between percent body fat and leptin (r: 0.271; p: 0,022) and there is a negative relationship


- 26 -

between percent body fat and adiponectin levels (r: -0,370; p: 0,002).

Conclusion: Waist circumference and percent body fat, negatively associated with adiponectin

levels. There are a correlation of fatty percent to leptin levels but with the waist circumference

unrelated.


Imperatorin Suppresses Esophageal Cancer Metastasis through Inhibition of

TGFβ2/ERKsignaling Pathway

Wen Wen Xu, Bin Li

Jinan University, Guangzhou 510632, China

Abstract—Imperatorinis abioactiveingredient extracted from the root of Angelica dahurica, a

traditional Chinese medicine. Imperatorin has been reported to have traditional curative

effects including anti-inflammatory, anti-coagulantand and anti-bacterial. Recent studies

showed that imperatorin may inhibittumor grow than dinduce apoptosis. However, it is still

unknown whether it can inhibit tumor invasion and metastasis. In addition, the effect of

imperatorin in ESCC cells remains unclear. Furthermore, the underlying mechanism show

imperatorin in fluences cancer cells were scarcely studied. This study aims to explore the

biological significance of Imperatorin in cancer invasion and metastasis, and illustrate the

molecular mechanisms involved. The in vitro and in vivo experiments demonstrated that

imperator in significantly suppressed the invasive and metastatic potential of ESCC cells

without significant toxic effects. Western blot analysis indicated that imperator in regulated

the expression levels of EMT markers including Fibronectin, N-cadherin and E-cadherin, and

inactivated the ERK signaling pathway. Bioinformatics analysis of proteomics data suggested

that TGFβ2 may play an important role in the biological functions of imperatorin, which was

confirmed by the decreases in expression and secretion of TGFβ2in imperatorin-treated ESCC

cells. Mechanistically, we found that Imperatorin may directly bind to CREB1, a known

upstream regulator of TGFβ2, and inhibit its phosphorylation and therefore

reduceTGFβ2transcription and expression. In summary, Imperatorin can reduce the

expression and secretion of TGFβ2 viaregulating CREB1, and then inhibit the

phosphorylation of ERK, as a result, suppressing the invasion and metastasis of ESCC

cells.[This work was supported by the National Natural Science Foundation of China

(81672953) and the Fundamental Research Funds for the Central Universities (21616322).]


Effects on Rats at Reflux Esophagitis Treated with Gardeniae Fructus 50% EtOH Extract

(GE)

Soo Hyun Kim, Seong-Soo Roh

Jinan University, Guangzhou 510632, China

Abstract—Objective: Gardeniae Fructus is a traditional herb that has been used to treat a

variety of diseases. In this study, antioxidant, anti-inflammatory and anti-apotosis properties

of reflux-induced esophagitis (RE) model in evaluated using Sprague-Dawley (SD) rats.

Materials and methods: The antioxidant activities were evaluated through radical scavenging


- 27 -

assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis

(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals. The rat was observed by

reflux-esophagitis (RE) after a 12-hour fasting period and was followed by a single

administration of 50 mg/kg or 100 mg/kg of GE.

The esophageal tissue was staining with Hematoxylin and eosin (H&E) and Periodic

Acid-Schiff (PAS).

Results: The application of the extract of the Gardeniae Fructus showed remarkable relief of

mucosal damage in esophageal tissue. In addition, the administration of GE reduced the

increase of serum reactive oxygen species (ROS). GE administration significantly reduced

inflammatory protein expression through MAPK-related signaling pathways and significantly

reduced the nuclear factor-kappa B (NF-κB) pathway.

Conclusion: The results of this study confirmed the reduction of inflammation by activating

the antioxidant pathway of GE in acute reflux esophagitis.

Session 4




Time: 16:00~17:45


7 presentations- Topic: “Environmental and Chemical Engineering”

Session Chair: Prof. Kokyo Oh


Phytoremediation of Heavy Metal Contaminated Soils with Maize of Different Varieties

Kokyo Oh, Shinichi Yonemochi, Miwa Makoto, Tetsushi Yonekura, Yugo Isobe, Hongyan

Cheng, Yinghe Xie, Jianping Hong, Tiehua Cao

Center for Environmental Science in Saitama, Saitama, Japan

Abstract— Soil contamination with heavy metals is a great public concern as it threatens

safety of agriculture, ecological environment, and human health. Phytoremediation as a

remedial technology and management option is recommended to solve the problem. Maize

(Zea mays L.) is a popular biofuel crop for ethanol production due to its abundance and

relative ease of conversion to ethanol, and has received increasing attention for heavy metal


- 28 -

removal from soils as its good economic income through ethanol production, large biomass

production, and easy cultivation and management. In this study, growth and phytoremediation

potential of 11 varieties of maize in a heavy metal contaminated soil were investigated using

pot culture to find the suitable maize varieties for phytoremediation of contaminated soils in

Japan.

The pot cultural experiment was conducted in the green house and the soil used was a heavy

contaminated loam soil with Pb, Ni, Cu and Cd. With three replications, 11 maize varieties

were grown and one plant in each pot was left for harvest finally. Plant dry biomass

production and heavy metal contents were determined, and phytoremediation potential was

evaluated based on the total amount of heavy metal extracted by plant in one pot.

The results showed that there were no great differences in total biomass yield. However, great

differences were found in grain yield among the 11 varieties, which ranged from 1.1 to 28

g/pot, showing that maize grain was more easily influenced than other plant parts by

contamination of heavy metals. The concentrations of heavy metals in maize were generally

in the order Cu>Cd>Pb, Ni. The 11 varieties generally had a similar level in heavy metal

concentrations, but the Bignikukon variety had a much higher Cd concentration than other

varieties. Phytoremediation potential of soil heavy metals among these varieties were found

varied greatly, and as an example the remediation potential of Cd ranged from 24.5 to143㎍

/pot. Two varieties, Kuromochitomorokoshi and Kakuteru600, were found possibly superior

in phytoremediation as they showed high levels in both phytoremediation potential and grain

production (This work was partially supported by JSPS KAKENHI No.16H05633).


Biochemical Characteristics of Alkaliphilic Cyclodextran Glucanotransferase for

Cyclodextran Production

So-Jin YANG, Hae-Soo KIM, So-Hyeon LEE, So-Yeon KIM, Min-Ho JO, Su-Jeong CHOI,

Ju-Hui KIM and Young-Min KIM



Abstract—Cyclodextran (CI) is a cyclic α-¬1,6 oligosaccharide produced from dextran by

cycloisomaltooligosaccharide glucanotransferase(CITase), and up to now, 7 to 17 linked CIs

have been reported. These CIs are over 100 times more soluble than cyclodextrin (CD). Also

CI has a wider molecular diameter than that of the α-1,4 bond of CD and has the ability to

encapsulate a guest molecule. For the industrial production of these CIs, we performed

production and purification of alkaliphilic CITase from Paenibacillus daejeonensis

(PDCITase). PDCITase showed the optimum activity at pH 8.0 and maintained 100% activity

for 18 h at pH 6.5-11.5, indicating that it was a alkaliphilic and alkali-stable enzyme.

PDCITase produces not only CI-¬7 to CI-¬17 but also CI-¬18 and CI-¬19. To our best

knowledge, these are new CIs that have not been discovered so far. In the enzyme kinetic

study, PDCITase were allosteric enzymes, unlike the existing CITase, and were characterized

by negative cooperative properties. These will be useful for CI production


- 29 -


Encapsulated Vitamin D3 in Liposome Coating by β-Lactoglobulin

Gemala Anjani, B.Panungga, D.N. Afifah, A.Pramono, M.Sulchan and A. Ohta


Abstract—Vitamin D3 (VD3) known as a lipid soluble vitamin is a important issue because

the high prevalence of deficiency in the world. However it is very delicate to oxygen, and

light. Encapsulating vitamin D3 by liposome in the form of small unilamellar vesicle

(SUV)-cholesterol coated by β-Lactoglobulin could be a significant answer in delivering VD3

to the target cells. Method VD3 concentration in system was estimated by HPLC. Size particle

and zeta potential were calculated by nano particle analyzer, DPPC content in system was

estimated by phospholipid C-enzyme assay. The solubility of VD3 decreased by the

cholesterol related to the similar active site of cholesterol and VD3 to DPPC. However, the

existences of β-Lactoglobulin in the liposome increase the solubility of VD3 in the system.

This study recommend to use 10% cholesterol in this 1.4 mM SUV with final concentration of

β-Lactoglobulin is 0.05 mM. Furthermore, it was concluded that the possibly interaction of

β-lactoglobulin in the liposome system is in surface of SUV.


Niger Delta Remediation-to-Biofuel Solution: Hydrocarbon Pollution Management to Wealth

Creation

Ayodele A. Otaiku

Faculty of Arts & Social Science, Department of Geography, Environmental Management

Nigeria Defence Academy, Kaduna, Nigeria

Abstract—Purpose: Convert degraded ecosystems of Niger Delta region into bioenergy

economy by 2020. An estimated 5-10% of Nigerian mangrove ecosystems have been wiped

out by hydrocarbon degradation. Today, decades since hydrocarbon was founded in Nigeria, a

wealth of completed reported and remediation cases offers us the opportunity to make a rich -

if preliminary -appraisal of the ―real‖ problems that underlie the remedy selection and

implementation process.

Methodology: The remediation-to-biofuel research and developed (2004-2016) technology

platform called PC3R Technology® (Pollution construct, Remediation, Restoration and

Reuse) for the sustainable development of the Niger Delta region into bioenergy economy.

PC3R

techniques encapsulates human capacity development, bioremediation, restoration ecology

(post-remediation) phyto-remediation and cultivation of bio-energy crops and bio-ethanol

production within the Niger Delta region. The case studies are: In 2007 remediation of OML

58, OB 12 /70 (Obagi) with microbial consortia treatment within four (4) weeks. Empty shells

of snails killed as a result of the toxicity of the drilling muds operations was restored by

improved ecology as a result of post restoration impact. Human capacity training Oil Spill

Management

and Environmental Remediation Training (OSMERT), May 5 -17th, 2014 using our PC3R

Technology®. Approval of climate smart technologies by Nigeria regulatory agencies 2015.

Findings: The process of implementing remediation and restoration as a Protocol for Niger


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Delta degraded ecosystems. An integrated solution from hydrocarbon cleans -to-wealth

creation (conversion of polluted land to agricultural farm land for bioenergy crops). The

techniques encapsulates human capacity development bioremediation, restoration ecology

(postremediation) phyto-remediation and cultivation of bio-energy crops converted into

bio-ethanol factory Niger Delta region as a wealth creation scheme.

Implication: PC3R Technology® provides the practical basis for assessing these problems,

and incorporating these new insights in ways that would improve the hydrocarbon operations

in Niger Delta region.

Conclusion: This is a framework and action plan for post remediation of Niger Delta region

where all stakeholders will become collaborators for sustainable development.


Acid Hydrolysis Gives the High Concentration of Gallic Acid in Green Tea Extract

Hae-Soo Kim, Gyeoung-Bin Park, So-Hyeon Lee, Min-Ho Jo, Su-Jeong Choi, Ju-Hui Kim,

Seung-Hee Nam, Jong-Bang Eun and Young-Min Kim



Abstract—Gallic acid is a functional ingredient abundantly present in puer tea, which is a

representative fermented tea of China. Recently, it has been reported that gallic acid has

physiological activities such as anti-oxidant, anti-obesity, anti-bacterial, anti-inflammation,

and the loss of body fat. Originally, green tea, a raw material of fermented tea, contains a

small amount of gallic acid but it has various kinds of catechins such as EGCG, ECG, EGC, or

EC. It has been studied to increase gallic acid through the fermentation or bio-transformation

of galloyl group in EGCG or ECG by tannase.

In this study, we tried to make green tea extracts increased gallic acid by acid hydrolysis

which is a simple and economical approach. The gallic acid was produced 45.6% yield from

EGCG with treatment of 2 M hydrochloric acid at 110°C for 1 h. When the same treatment

against the green tea extracts from Chamnok as a cultivated in Korea, native species, and

Yabukita, the gallic acid increased from 0.17 mM into 4.87 mM (91.3% yield), from 0.28 mM

to 5.33 mM (92.3% yield), and from 0.17 mM to 4.44 mM (93.9% yield), respectively.

Therefore, it is possible to manufacture green tea extract with high concentration of gallic acid

by using simple acid hydrolysis.


Simulation of Soil Failure around the Subsoiler Type Curved Leg using Computational Fluid

Dynamics

Samart Bun-Art and Kriengkrai Rayanasuk

Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand

Abstract—Use of soil tillage tool in agricultural field wants for soil failure. Behavior of soil

failure: soil deformation and soil failure depend on soil properties and action force method.

The subsoiler used to change the depth of soil from the surface to the ground breaking into a

small block. The objective of this research was to determine soil flow pattern around the

subsoiler type curved leg used computational fluid dynamics and to study soil fracture and


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soil movement of subsoiler type curved leg. Simulations carried out using ANSYS Fluent

14.0 commercial CFD software. The computational fluid dynamics used predict the soil flow

through subsoiler type curved leg at subsoiler operating speed constant. For a subsoiler speed

of 1.02 m s-1

, with operating at 400 mm depth, rake angle of 30°. The results show, the soil

failure is lateral deformation of subsoiler and soil movement on the surface of rake angle.


Data Engineering Analysis of Fruit Bug Characteristics for Pear Cultivation

Kazuya Miyakawa and Tatsuya Yamazaki

Niigata University, Niigata, Japan

Abstract—Several kinds of fruits are covered with fruit bags until harvesting to protect them

from insects, birds, disease, agrochemical, etc. It is called as the preharvest bagging. There

may be negative effect of the fruit bag used in the preharvest bagging, because humidity in the

fruit bag is possible to rise. In this paper, the inner-bag temperature and humidity are directly

measured at hourly intervals for four months from infancy to harvesting. During

measurement, six different types of fruit bags are used. The collected measurement data are

statistically analyzed, and difference is significantly disclosed among these inner-bags for

humidity.

Poster Session




Time: 08:55~17:45


Poster Session: 3 presentations

C3001 Poster 1 (08:55~17:45)

Preparation and Physicochemical Properties of the Isomaltooligosaccharides Using

Puffed-rice Flour

Bo-Ram Park, Han Suk Choi and Shin-Young Park

Department of Agrofood Resources, National Institute of Agricultural Science, Rural

Development Administration, Korea


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Abstract—This study was conducted to investigate the physicochemical properties of

isomalto-oligosaccharides using puffed rice flour by one-step processing method under

different manufacturing condition. Puffed rice flour sample prepared with grounded puffed

rice flour by passing through 50 mesh standard seive. Experimental groups were composed

according to substrate concentration(15%, 20%, w/v) and reaction temperature(60℃, 65℃);

PR15-60, PR20-60, PR15-65, PR20-65. Enzyme reaction of puffed rice flour and water

mixture during 0, 1, 6, 24, 48 hours with commercial enzymes (Maltogenic, Promozyme D2

and Transglucosidase) amount of 0.5% (w/v), respectively. We evaluated the physicochemical

properties of production of isomalto-oligosaccharides regarding to different manufacturing

condition. Reducing sugar contents, dextrose equivalent (DE), and total soluble solides of

reactant increased according to time goes by. The carbohydrate composition and contents of

isomaltooligosaccharides using puffed rice flour containing DP(degree of polymerization) 2

to DP 7 was examined by a HPLC-ELSD(evaporative light scattering detector) system. As a

result of the carbohydrate composition and quantitative analysis, experimental group PR20-65

has highest isomalto-oligosaccharides contents at 6 hour(138.47 mg/mL) and

isomaltooligosaccharides / total carbohydrate ratio at 24 hour sample, 135.00 mg/mL and

68.04 %, respectively. B0005 Poster 2 (08:55~17:45)

The Current State of Custom Works in Agriculture of the Republic of Korea

Jeongmin Lee, Yuyong Kim, Byounggap Kim

National Institute of Agricultural Sciences, R.D.A., Jeonju, 55365, Korea

Abstract—The lack of farm machinery operators in rural areas in Korea in the near future due

to the aging agricultural population is causing concern as this will disrupt timely farm work.

The present study analyzed the statistics of the agricultural census by Statistics Korea and

determines the current state of custom works of farm villages to set the direction for

improvement of the system in using farm machinery. The ratio of custom work of domestic

rice cropping is a growing trend. Especially, raising seeding and pest control need a lot of

work forces and their custom works increased a lot. Also, the ratio of custom work by size of

farm was the highest in 3ha or smaller farm households at 45.8% to 85.2%. And the farm

owner whose age was 65 or older was high at 4.0∼20.9%p per work. The ratio of farm

households with the farm owner whose age was 65 or older increased from 43% in 2005 to

54% in 2015, showing an 11%p increase. Farms with older owners tended to show a higher

ratio of custom work, which raises concern about disruption to timely farm work due to aging.

Therefore, it is necessary to come up with a measure against the constant decrease in farm

machinery operators due to aging. In addition, to overcome the lack of farm machinery

operators in the future and smoothly carry out farm work in time, it is necessary to establish

preemptive measures to improve the system of using farm machinery such as setting up an

agency for farm work and a business specialized in mediating custom work.


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B0006 Poster 2 (08:55~17:45)

Case Study on a Rainwater Catchment Device for Water Harvesting in Upland Farm in Long

Slope Land

Youngjin Kim, Yonghun Choi, Minyoung Kim and Jonggil Jeon

National Institute of Agricultural Sciences, Jeonju-si, Republic of Korea

Abstract—Korea has been damaged in agriculture production in almost every 2-year for last

10 years. The reason of that can be appointed of rainfall pattern exchange and upland area

promotion. Even though the annual total precipitation is increasing, the drought damages

production of vegetables and crops in upland farm. Most of Korean upland field area under

0.5 ha is hard to be irrigated with large reservoirs and water channel networks. The purpose of

this study is to develop a water catchment device capable of collecting rainwater runoff flow

in small stream channel, which can be helpful to irrigate small upland field of 1,000 m2 area.

The system is consisted with a catchment device and a storage tank. Furthermore, this water

harvesting system operation and effect were tested in a field farm.

The rainwater catchment device has cylindrical shape of 150 mm diameter and 700mm length

that has slits of water inflow on its surface. This stainless steel device can ensure strong and

non-corrosion of exterior shocks, Slit on its surface can separate large amounts of suspended

solids. A filter media was inserted into the device inner space by a size of 120cm × 50 cm.

The maximum intake flow was analyzed in the test channel. This catchment device was

installed in natural small channel, and its catchment flow was monitored with cumulative

water meter. The real time total runoff flow was measured with V-notch flume and floating

water level sensor.

The maximum intake flow was analyzed in the test under various runoff flow rate and level.

The total flow rate was varied in 143.4 L /min, 263.5 L/min and 489.4 l / min, while varying

the water level from 5 to 30 cm at each specific flow rate. The maximum intake flow of the

device is 142 L/min. The devices were installed at two points of mountain small stream for

field performance analysis. Point 1 is to rainfall runoff flow. Point 2 is to ordinary runoff.

Total rainfall depth for this study was 334.5 mm. Total runoff flow of 1,722 ton and 7,984 ton

occurred in point 1 and point 2, then 273 ton and 125 ton were collected by this rainwater

catchment device. It was possible to 30 ton/month of rainwater harvesting and it is expected

that the rainwater catchment device will help to solve short-term water shortage in a field of

roughly 10 area. The catchment ratio to runoff flow is less than 20 %, which means that

periodic maintenance is required for eliminating the removal of dried dwellings and removing

deposits of sedimentary sediments. This water harvesting system can catch 5 tons of water

from 1 mm rainfall depth on 1 ha area.

This wok was conducted with the support of the ―Research Program for Agriculture Science

& Technology Development (Project No. PJ012686012018)‖, National Institute of

Agricultural Sciences, Rural Development Administration, Republic of Korea.


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Listeners Name Affiliation

Prof. Jong-Bang Eun Chonnam National University, REPUBLIC OF KOREA

Ju-Hui Kim Chonnam National University, REPUBLIC OF KOREA

Yongmei Jin Jilin Academy of Agricultural Sciences, China

Xiufeng Lin Jilin Academy of Agricultural Sciences, China

Loke-Ming Chou National University of Singapore, Singapore

Kriengkrai Rayanasuk Suranaree University of Technology, Thailand

Win Chaeychomsri Kasetsart University, Thailand


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One Day Visit Time: 8:50~17:00 March 30, 2018(Friday)

(Tip: The following places are for references, and the final schedule should be adjusted to the

actual notice.)

8:50 Gathering in Daiwa Roynet Hotel Naha Omoromachi’s lobby

1. 9:00 depart from the hotel

2. 9:30~10:30 Urasoe Large Park

This is one place in Okinawa where your kids will love to come

repeatedly to enjoy the rides and the nice atmosphere. There are

plenty of sliding and climbing games for the kids and you will be

amazed to see the facility for the toddlers. You can also enjoy

some rides here, with the roller coaster being the best choice. The

sandbox area is developed only for the kids and your child will

love to play with some new friends here. You must try to reach the

top of the building where you can enjoy a majestic view of the entire area. Don’t miss out

on the delicious ice-creams from the parlour in the park.

3. 11:00~12:00 Zampamisaki

Zampamisaki is located at the northwestern end of

Yomitani-mura. It is a famous tourist attraction in Okinawa. White

lighthouse, blue sea and blue sky, beautiful scenery. There are

promenades around the wreckage for walks, and there are also

famous attractions such as diving and seaside fishing.

4. 12:30~13:30 Lunch

5. 14:00~14:30 Naminoue Shrine

Naminoue Shrine, literally "Above the Waves Shrine", is a Shinto

shrine in Naha, Okinawa, Japan, the primary shrine in the

prefecture. It sits atop a high bluff, overlooking Naminoue Beach

and the ocean.

Originally a sacred space of the native Ryukyuan religion, due to

its location and natural beauty, it was dedicated to nirai kanai, the mythical source of all life,

and to the sea. At some point it came to be known as Hana gusuku and Nanminsan;

6. 15:30~17:00 Shuri Castle

Shuri Castle is a Ryukyuan gusuku in Shuri, Okinawa.

Between 1429 and 1879, it was the palace of the Ryukyu

Kingdom, before becoming largely neglected. In 1945, during

the Battle of Okinawa, it was almost completely destroyed.

After the war, the castle was re-purposed as a university

campus.

7. 17:00 Go back to Naha City


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Conference Venue

Faculty of Engineering, University of the Ryukyus, Japan https://ie.u-ryukyu.ac.jp/

Address: 1 Senbaru,Nishihara, Okinawa Japan 903-0213

The University of the Ryukyus was established in 1950 on the remains of Shuri Castle, which

was burnt to ashes in the Battle of Okinawa. Under the U.S. administration governing

Okinawa, the strong desire of Okinawans for postwar reconstruction and restoration of

education moved the U.S. Military Government to establish the first university in the Ryukyu

Archipelago. In 1966, administration of the University was transferred to the Ryukyu

Government, and with the reversion of Okinawa to Japan in 1972, the University became a

national university. In 1977, the University was moved to a large new campus in the region

where the three municipalities of Nishihara Town, Ginowan City, and Nakagusuku Village

meet, and it became a national university corporation in 2004. Although the University of the

Ryukyus has gone through a number of historic hardships, such as interference in academic

freedom and the autonomy of the university during such transitions, it has made great

contributions to the development of human resources in the local community and to the

production of new knowledge.


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Note


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Note


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Note


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Documents

2018 9th International Conference on Food Engineering and ...icfeb.org/ICFEB2018-program.pdf · Ninik Rustanti 21 C3004: Prohibited Coloring Agent in Dominating Hazardous Street Food