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TRANSPIRATION : LIGHT INTENSITY
Design an experiment to investigate whether light intensity affects the rate of transpiration by using a simple photometer
Problem statement : How does the light intensity affect the rate of transpiration?
Objective : To investigate whether light intensity affects the rate of transpiration
Hypothesis : The rate of transpiration of plant is higher in strong sunlight
Variable
Manipulated variable : Light intensityResponding variable : Rate of movement of air bubble in the photometerFixed variable : air movement, temperature and relative humidity
Materials and apparatus:
Capillary tube (about 30 cm long), retord stand, 500 ml beaker, basin, scalpel, rubber tubing (about 6 cm long), tissue paper, vaseline, marker pen and stop watch, ruler , 2 Hibiscus plant of similar size
Technique : The movement of water in a given time is recorded using a stopwatch.
Procedure
1. The leafy shoot is immersed in a basin containing water, and its stem is cut diagonally
2. The leafy shoot is placed upright in the phptpmeter’s capillary tube, through a stopper cork
3. All equipment connection points are wiped with vaseline4. To trap an air bubble, the end of the capillary tube is removed from the basin, and
then put back into the water5. A length of 5 cm is marked on the photometer6. The photometer is placed in the shade and using a stopwatch, record the time taken
(in minutes) for the air bubble to move from point A to point B 7. To reset the photometer, squeeze the rubber tubing so that the air bubble escapes
into the beaker of water.8. Repeat step 4 to 7 to get three readings with the same shoot in the shade and under
strong sunlight respectively9. The average and the rate of transpiration measurement is recorded in the table.
Result:
Environmental condition of experiment
Time taken for air bubble to move from Point A to Point B (in minutes)
Rate of transpiration(cm/min)First
readingSecond reading
Third reading
Average
Shady (lower light intensity)
Strong sunlight (higher light intensity)
Conclusion: Hypothesis is accepted. In a shady condition, the plant’s rate of transpiration is lower
when compared with its rate of transpiration in strong sunlight
.