126 PATENT ABSTRACTS New antibiotically active compounds having the basic structure of rifamycin S. namely 3-hydroxyrifamycin S (formula A: X= (Cc double bond@ Rl=OH; RZ=H). 3. 31- dihydroxyrifamycin S (formula A: X=(C double bond0; RI=RZ=OH) and I-desoxy-l- oxarifamycin S (formula A: X=-O-; RI= R2=H) (A) are formed by cultivating, under aerobic conditions, a strain of Nocardia mediterranei which is derived from Strep- tomyces mediterranei ATCC 13 685 as the parent strain and is characterized by the ability to produce at least one of the mentioned compounds. The recombinant strain Nocardia mediterranei DSM 1415 has proved suitable. The mentioned rifamycin S analogues have analogous antibiotic proper- ties to this but have a wider range of action. especially against gram-negative bacteria. 4376110 IMMUNOMETRIC ASSAYS USING MONOCLONAL ANTIBODIES Gary S. David. Howard E. Greene, assigned to Hybritech incorporated I,,,, -- 9, CI” Two-site or sandwich immunometric assay techniques for determination of the presence and/or concentration of antigenic substances in fluids using monoclonal antibodies. One monoclonal antibody is presented in a soluble labeled form and a second monoclonal antibody is presented bound to a solid carrier; the soluble and bound monoclonal antibodies may be the products of either the same or different cell lines. Each monoclonal antibody has an affinity for the antigenic substances of at least about 108 liters )I mole. 4376071 MITOGENIC SPINAL CORD GROWTH FACTOR Thomas J. Jennings. Allan Lipton. assigned to Research Corporation A proteinaceous factor substantially puri- fied from mammalian spinal cord which has mitogenic activity, a molecular weight of about 11,000. a pl of 9.6 and which is heat and acid labile, is useful for the promotion of growth of cells. 4376059 PROCESS FOR PREPARING ARTIFICIAL RED CELLS Thomas A. Davis. William J. Asher. assigned to Exxon Research and Engineering Co A process for preparing artificial red cells comprising microdroplets of aqueous, stroma- free hemoglobin solution encapsulated in membranes of polymerized hemoglobin. said process comprising forming microdroplets of hemoglohin solution in a continuous oil phase. cross-linking the hemoglobin at the surface nf the microdroplets with a suitable cross-linkmg agent to form the artificial red cells and recovering the cells. In one embodiment. a liquid membrane encapsulation technique employing a water-soluble cross- linking agent is used to form the cells. In another embodiment an oil-soluble cross- lurking agent is present in the oil phase con- taining the hemoglobin microdroplets.

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Page 1: 4376059 Process for preparing artificial red cells

126 PATENT ABSTRACTS

New antibiotically active compounds having the basic structure of rifamycin S. namely 3-hydroxyrifamycin S (formula A: X= (Cc double bond@ Rl=OH; RZ=H). 3. 31- dihydroxyrifamycin S (formula A: X=(C double bond0; RI=RZ=OH) and I-desoxy-l- oxarifamycin S (formula A: X=-O-; RI= R2=H) (A) are formed by cultivating, under aerobic conditions, a strain of Nocardia mediterranei which is derived from Strep- tomyces mediterranei ATCC 13 685 as the parent strain and is characterized by the ability to produce at least one of the mentioned compounds. The recombinant strain Nocardia mediterranei DSM 1415 has proved suitable. The mentioned rifamycin S analogues have analogous antibiotic proper- ties to this but have a wider range of action. especially against gram-negative bacteria.

4376110

IMMUNOMETRIC ASSAYS USING MONOCLONAL ANTIBODIES

Gary S. David. Howard E. Greene, assigned to Hybritech incorporated

I,,,,

9, CI”

Two-site or sandwich immunometric assay techniques for determination of the presence and/or concentration of antigenic substances in fluids using monoclonal antibodies. One monoclonal antibody is presented in a soluble labeled form and a second monoclonal antibody is presented bound to a solid carrier; the soluble and bound monoclonal antibodies may be the products of either the same or different cell lines. Each monoclonal antibody has an affinity for the antigenic substances of at least about 108 liters )I mole.

4376071

MITOGENIC SPINAL CORD GROWTH FACTOR

Thomas J. Jennings. Allan Lipton. assigned to Research Corporation

A proteinaceous factor substantially puri- fied from mammalian spinal cord which has mitogenic activity, a molecular weight of about 11,000. a pl of 9.6 and which is heat and acid labile, is useful for the promotion of growth of cells.

4376059

PROCESS FOR PREPARING ARTIFICIAL RED CELLS

Thomas A. Davis. William J. Asher. assigned to Exxon Research and Engineering Co

A process for preparing artificial red cells comprising microdroplets of aqueous, stroma- free hemoglobin solution encapsulated in membranes of polymerized hemoglobin. said process comprising forming microdroplets of hemoglohin solution in a continuous oil phase. cross-linking the hemoglobin at the surface nf the microdroplets with a suitable cross-linkmg agent to form the artificial red cells and recovering the cells. In one embodiment. a liquid membrane encapsulation technique employing a water-soluble cross- linking agent is used to form the cells. In another embodiment an oil-soluble cross- lurking agent is present in the oil phase con- taining the hemoglobin microdroplets.