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140 PATENT ABSTRACTS
Peptides having the following sequences are dis- closed: Leu-Asp-Gln-Phe-Pro-Leu-Gly-Arg- Lys-Phe-Leu, Leu-Asp-Gln-Phe-Pro-Leu-Gly- Arg-Arg-Phe-Leu, Ala-Lys-Arg-Arg-Arg-Lys. and Ala-Lys-Lys-Lys-Lys-Lys.
4551433
MICROBIAL HYBRID PROMOTERS
A method is described for constructing double- stranded DNA using messenger RNA templates. DNA strands complementary to the templates are formed using a non-specific Y hydroxy ter- minus primer. After separation, the DNA single strands are disabled from self priming by blocking the free 3 ~ hydroxy. Primer segments are then provided complementary to a portion of the amino terminus-coding end of the desired single strands. These primer segments are then extended with a suitable polymerase to complete the complementary strands.
Herman A DeBoer assigned to Genentech Inc
Novel microbial hybrid promoters and their use to direct the microbial expression of hetero- logous genes are described. Such promoters are selectively and functionally constructed by recombinant techniques, utilizing the discovery that certain DNA regions of given promoters are responsible for particularly advantageous func- tional properties.
4559300
M E T H O D F O R U S I N G A N H O M O L O G O U S B A C I L L U S
P R O M O T E R A N D A S S O C I A T E D N A T U R A L O R M O D I F I E D
R I B O S O M E B I N D I N G S I T E - C O N T A I N I N G D N A S E Q U E N C E IN
S T R E P T O M Y C E S
4554250
M E T H O D O F I N C R E A S E D P R O D U C T I O N O F P E N I C I L L I N
A C Y L A S E A N D P L A S M I D E M P L O Y E D T H E R E I N
John E McCullough assigned to E R Squibb & Sons Inc
A method is provided for enhancing penicillin acylase production wherein a Bacillus subtilis plasmid containing an insert of a chromosomal DNA fragment of Bacillus megaterium which in- cludes the appropriate gene for enhancing penicillin acylase production is cloned into Bacil- lus megaterium and the Bacillus megaterium containing the above plasmid is employed in the production of penicillin acylase. A plasmid method for forming same and host plasmid com- bination which are useful in the above method are also provided.
Steven Kovacevic, Jeffrey T Fayerman, James Miller, Mark A Richardson assigned to Eli Lilly and Company
A method for expressing a functional poly- peptide in Streptomyces comprises transforming a Streptomyces host cell with a recombinant DNA expression vector and then culturing the transformed cell under conditions suitable for cell growth. The recombinant DNA expression vector comprises the veg or any other homo- logous Bacillus promoter, a naturally occurring or modified ribosome binding site-containing DNA sequence and a gene that codes for a func- tional polypeptide such as human pre- proinsulin. The method is specifically exemplified by use of expression plasmids pOW529, pOW539 and transformants, Strep- tomyces ambofaciens/pOW529 and Streptomy- ces ambofaciens/pOW539. The method is broadly applicable and is particularly useful in economically important Streptomyces taxa.
4559308
4555486
M E T H O D F O R U S I N G A N A M I N O - T E R M I N U S D N A
S E Q U E N C E T O S Y N T H E S I Z E A S P E C I F I C D O U B L E - S T R A N D E D
D N A
Chancier Bahl, Jack Nunberg assigned to Cetus Corporation
C O R Y N E B A C T E R I U M P L A S M I D A N D V E C T O R
Robert C Nutter, Lucy C Panganiban assigned to Stauffer Chemical Co
Plasmids derived from Corynebacterium glutamicum, particularly pRN3.1, of suitable size, about 3,1 kilobases in length and weight ab- out 2.0"106 daltons, and having a limited num- ber of restriction sites therein, suitable as vehicles for genetic engineering of Corynebacterium.