354 PATENT

protein therefor; (b) a conjugate comprising the other partner of said specific binding pair in- corporated with a substance which protects the specific binding protein of said pair from enzyme inactivation when bound with its partner: and (c) an active protein-inactivating enzyme. Also a specific binding method of assaying for an enzyme-resistant ligand in a sample, which method uses the above test material and which results in a reduction in interference caused by non-specific protein.

4650760

M O D I F I E D A L K A L I N E P H O S P H A T A S E

Jan F Chlebowski, Catherine H Roberts as- signed to Research Corporation

This invention provides a proteolytically modified alkaline phosphatase which is irrever- sibly inactivated upon removal of divalent ions. The modified enzyme is particularly useful as a molecular biological or immunological reagent.

4650763

PROTEASE AND PROCESS FOR PRODUCTION AND USE

THEREOF

Hisayuki Matsuo, Kensaku Mizuno, Takaharu Tanaka, Miyazaki, Japan assigned to Suntory Limited

Disclosed is a new protease having the following properties: (1) it is able to hydrolitically cleave a peptide bond between two adjacent basic amino acids in a peptide chain; (2) it has a molecular weight of about 43, 000 as determined by elec- trophoresis; (3) it is inhibited by phenyl- methylsulphonyl fluoride and diisopropyl fluorophosphate, but is not inhibited by mono- iodoacetate, p-chloromercuribenzoic acid, et hylenediaminetetraacetic acid. 1.10- phenanthroline, tosyl-L-lysine chloromethyl ketone, and leupeptin. The protease can be pro- duced by culturing Saccharomyces cerevisiae, and recovering purification by conventional methods, and is useful as a processing enzyme for conversion ofa prohormone to an active hor- mone.

ABSTRACTS

4652524

S O L U B L E S T A B I L I Z E D E N Z Y M E S

Ivan E Modrovich. Paul F Wegfahrt, Wanda A Gaussiran assigned to Modrovich Ivan E

A method is disclosed for preparing a soluble stable enzyme. The method comprises the steps of reacting in a liquid media an enzyme with a polymer having pendant groups capable ofcova- lently bonding with pendant groups on the en- zyme. The enzyme and polymer are mixed with at least one composition which affects the ac- tivity of the enzyme. Such a composition can be selected from the enzyme substrate, product of the enzyme substrate reaction, an activator for the enzyme and'or inhibitor for the enzyme. In addition, a composition can be added which can also competitively react with the available pen- dant groups of the polymer.

4654300

F L U O R E S C E N T M I C R O B E A D Q U E N C H I N G A S S A Y

Robert F Zuk, David J Litman assigned to Syn- tex (U S A ) Inc

Method and compositions for performing immunoassays having conjugated fluorescent particles and conjugated catalyst where the par- ticles and catalysts are conjugated to members of a specific binding pair. Bound to the fluorescent particle is a catalyst, usually enzyme, member of a signal producing system, which system in- cludes the fluorescent particle. Another catalyst is bound to a specific binding pair member. The catalyst-specific binding member conjugate becomes bound to the particle, by the inter- mediacy of the binding of the specific binding pair, producing a quenching product which binds to the particle, resulting in a reduction in fluorescence.

4656128

M O D I F I E D A L K A L I N E P H O S P H A T A S E

Jan F Chlebowski, Catherine H Roberts as- signed to Research Corporation

This invention provides a proteolytically modified alkaline phosphatase which is irrever-