PATENT

molecular weight about 28,000 (gp 28) and a pro- tein of molecular weight about 23,000 (p 23), ob- tained by known methods by treatment of surface antigen with detergent, are prepared in micelle form substantially free from detergent. The micelles are at least as immunogenic as the unfragmented antigen from which they are derived and are useful in the preparation of hepatitis B vaccine. The detergent is removed by layering the detergent containing fragmentation product on an aqueous buffer containing a sucrose gradient and centrifuging the layered buffer when micelles containing both gp 28 and p 23 form and can be recovered in substantially detergent free form from the centrifuged layered buffer.

4666845

M O N O C L O N A L A N T I B O D I E S T O O V A R I A N , C E R V I C A L A N D

U T E R I N E H U M A N C A N C E R S A N D M E T H O D O F D I A G N O S I S

M Jules Mattes, John L Lewis, Kenneth O Lloyd, Lloyd Old, Carlos Cordon-Cardo as- signed to Sloan-Kettering Institute

Mouse monoclonal antibodies to several cell antigens of human ovarian, cervical and endo- metrial carcinomas have been produced and characterized. The distribution of the antigens was determined by mixed hemagglutination assays on 153 normal and malignant cell cultures of various types, and by immunoperoxidase staining of frozen sections of 27 normal adult and 24 fetal tissues, five monoclonal antibodies representative of five classes of mAb raised to restricted ovarian, cervical and endometrial cells were tested extensively producing mAb reactive with cancer but not normal cells. One such mAb, MF116 was readily detected in the spent culture medium of metabolically radiolabeled cells. These antibodies, reacting with relatively restric- ted cell surface antigens, are useful in the analysis of epithelial cell differentiation, in cancer dia- gnosis and therapy and in tissue typing of normal or abnormal cells.

4666846

N O V E L C L O N I N G V E C T O R S C O N T A I N I N G S E L E C T A B L E

G E N E T I C M A R K E R S F O R U S E IN S T R E P T O M Y C E S A N D R E L A T E D

O R G A N I S M S

Jeffrey T Fayerman, Michael D Jones, Nancy Malin assigned to Eli Lilly and Company

ABSTRACTS 371

The present invention discloses novel recom- binant DNA cloning vectors for use in Strep- tomyces and related organisms. These novel cloning vectors contain genetic markers that provide antibiotic resistance or colorimetric selectivity to the host cells. The invention further comprises transformants of the aforementioned vectors.

4668621

D E T E C T I N G B L O O D C L O T T I N G F A C T O R S W I T H I M M O B I L I Z E D

F I B R I N O G E N A N D L A B E L E D F I B R I N O G E N

George J Doellgast assigned to Wake Forest University

Assays and reagents for the direct determination of blood factors, as well as complementary methods and reagents for determining such blood factors and haptens, antigens and recep- tors. The methods involve clot formation due to thrombin activated fibrin formation from in- solubilized fibrogen and labeled solubilized fibrogen. Insolubilized label can be determined prior to or after clot formation.

4668629

H U M A N H Y B R I D O M A S , P R E C U R S O R S A N D P R O D U C T S

Henry S Kaplan, Lennart Olsson assigned to The Board of Trustees of Leland Stanford Jr University

Human monoclonal antibody compositions, human-human monoclonal hybridoma cells, human myeloma cells, human antibody genes and their uses. Human myeloma cells are developed for fusing with immunized lymphoid cells to provide stable human-human hybridoma strains producing complete monoclonal anti- bodies for a predefined antigen. From a myeloma cell line, rapidly growing 8-azaguanine resistant HAT sensitive cells are selected. The selected myeloma cells are crossed with im- munized lymphoid cells and the resulting cell mixture grown under controlled selective condi- tions. After expansion of the desired hybridoma cells, the monoclonal antibodies may be har- vested. The hybridomas serve as a source for messenger RNA for light and heavy chains which may be used for production of light and