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4769330

PATENT ABSTRACTS

M O D I F I E D V A C C I N I A V I R U S A N D M E T H O D S F O R M A K I N G

A N D U S I N G T H E S A M E

Enzo Paoletti, Dennis Panicali assigned to Health Research Incorporated

What are disclosed are methods for modifying the genome of vaccinia virus to produce vaccinia mutants, particularly by the introduction into the vaccinia genome of exogenous DNA; modified vaccinia prepared by such methods; certain DNA sequences and unmodified and genetically modified microorganisms involved as intermediates in such methods; and methods for infecting cells and host animals with such vaccinia mutants to provoke the amplification of exogenous DNA and proteins encoded by the ex- ogenous DNA, including antigenic proteins, by said cells and host animals.

A process for the preparation of a mutant strain of B. bronchiseptica having chromosomal gene- tic markers carrying at least temperature- sensitive (grown at 42 degrees C.), nalidixic acid- resistant and heat-labile dermonecrotic toxin producing ability negative markers, suitable for the preparation of live attenuated AR vaccine, which comprises subjecting a strain of B. bronchiseptica to an induced mutation, isolating a mutant having the genetic markers of being at least temperature-sensitive (grown at 42 degrees C.), nalidixic acid-resistant and heat-labile der- monecrotic toxin negative, culturing the isolated strain of B. bronchiseptica on a Bordet-gengou agar plate containing nalidixic acid at 42 degrees C., thereafter picking up an organism morpho- logically resembling a phase-I organism, repeating the procedures of culturing and picking up, and converting the organisms to a heat-labile dermonecrotic toxin-producing ab- ility negative phase-! organism having a com- plete capsular antigen.

4769331

R E C O M B I N A N T M E T H O D S A N D M A T E R I A L S

Bernard Roizman, Leonard E Post assigned to University Patents Inc

Specific DNA sequence insertions, deletions and substitutions (i.e., combinations of sequence deletion and insertion) in eukaryotic cell or viral genomes are stably effected through use of selec- table DNA sequences comprising a herpesvirus thymidine kinase (tk) gene.

4770875

P R O C E S S F O R P R E P A R A T I O N O F I M P R O V E D M U T A N T S T R A I N O F B O R D E T E L L A B R O N C H I S E P T I C A U S E F U L F O R L I V E A T T E N U A T E D V A C C I N E F O R P R O T E C T I O N O F

B. B R O N C H I S E P T I C A I N F E C T I O N A N D L I V E A T T E N U A T E D A R

V A C C I N E P R O D U C E D T H E R E F R O M

Katsum Kume, Toyotsug Nakai, Hiroshi Nishizawa, Takashi Yoshikawa, Hirofumi Dan- bara, Chiba, Japan assigned to The Kitasato In- stitute

4770992

D E T E C T I O N O F S P E C I F I C D N A S E Q U E N C E S B Y F L O W

C Y T O M E T R Y

Engh Gerrit J Van den, Barbara J Trask

A method for detection of DNA sequences. In the method, chromatin (comprising protein and DNA) is contacted with a cross-linking agent for the protein of the chromatin to provide a sub- stantially rigid chromatin particle. The DNA of the chromatin particle is then subjected to treat- ment to cause a separation of the individual DNA strands into single stranded DNA. Pre- selected sequences of the singlestranded DNA are then contacted with a complementary poly- nucleotide probe specific for the DNA sequence of interest. The polynucleotide probe is marked with a fluorescent label thereby labelling a target sequence of DNA in the chromatin particles. The fluorescently tagged DNA sequences are then detected by subjecting the polynucleotide probe to a suitable light source and detecting the light emitted by the fluorescent label so as to identify the preselected DNA sequence.