PATENT ABSTRACTS 399

4839291 4839419

D I S P O S A B L E B I O L O G I C A L M E T H O D F O R I M M O B I L I Z I N G I N D I C A T O R T E S T P A C K F O R D I S S O L V E D P R O T E I N S

M O N I T O R I N G S T E A M A N D E T H Y L E N E O X I D E Dieter Kraemer, Hermann Plainer, Bruno

S T E R I L I Z A T I O N C Y C L E S Sproessier, Helmut Uhlig, Reiner Schnee, Mainz, Federal Republic Of Germany assigned

Jon D Welsh, Denis G Dyke assigned to to Rohm GmbH American Sterilizer Company

Methods for adsorbing a protein, for example an A disposable test pack for monitoring the el'- enzyme, onto an insoluble, solid, macroporous, ficacy of steam or ethylene oxide sterilization small-particle support by washing said support cycles includes a fiber board housing for a bio- with an aqueous solution of the protein con- ligical indicator. The housing includes an outer raining an electrolyte in an ionic strength of at tube and a shorter inner tube. The outer tubehas least 0.15 mole/liter and cr6sslinking said pro- upper and lower portions which define a seam or tcin, before, during, or after such adsorption, gap at their adjoining open ends. The inner tube with a coupling component present in aqueous extends past the seam and telescopes into the up- electrolyte-containing solution. per and lower portions. The seam and the close tolerance between the inner tube and the upper and lower portions of the outer tube provide a 4839439 tortuous path for the entry of sterilant into the interior of the housing. The tortuous path has a P R E P A R A T I O N O F P O L Y M E R moisture absorbent surface and is dimensioned B A S E D O N P O L Y V I N Y L E N E to promote intimate contact between the C A R B O N A T E AND/OR sterilant and the absorbent surface. The exterior P O L Y H Y D R O X Y M E T H Y L E N E of the housing can be lined with foil to prevent sterilant from permeating the housing, thereby Otto Mauz, Liederbach, Federal Republic Of preventing entry of the steam by any path other Germany assigned to Hoechst Aktiengesellc- than through the seam. A hole covered by an op- shaft tionally removable tab is provided at the end of the upper portion of the outer tube for use in The invention relates to modified polymers monitoring ethylene oxide sterilization cycles: based on polyvinylene carbonate and/or poly-

hydroxymethylene, the modification being ef- fected by particular alkoxylated compounds incorporated into the polymer. These polymers, for the preparation of which, according to the in- vention, particular dispersion stabilizers are

4839292 used, are suitable, inter alia, as carriers for bio- logically active substances or for affinity

C E L L C U L T U R E F L A S K chromatography. U T I L I Z I N G A M E M B R A N E

B A R R I E R 4840714

Joseph G Cremonese E L E C T R O B L O T T I N G

A cell culture flask includes a hollow housing T E C H N I Q U E F O R and a gas permeable membrane disposed within T R A N S F E R R I N G S P E C I M E N S the housing which divides the housing into a first F R O M A P O L Y A C R Y L A M I D E chamber and a second chamber. A retaining ring E L E C T R O P H O R E S I S O R L I K E secures the gas permeable membrane within the G E L O N T O A M E M B R A N E housing and forms a liquid-tight seal between the first chamber and the second chamber. A gas in- William J Littlehales assigned to American Bio- let in the housing communicates with the first netics Inc chamber and supplies gas thereto. A media inlet in the housing communicates with the second An apparatus especially suitable for transferring chamber and provides cell growth media thereto, a particular specimen from a polyacrylamide

40o PATENT ABSTRACTS

electrophoresis or like gel onto a support sheet Describes a process for controlling the poly- by means of electroblotting is disclosed herein merization and cross-linked density of elec- along with its method of operation. This ap- trophoretic gel products useful for separation of paratus utilizes a pair of plate-shaped electrode bioorganic molecules, which process does not assemblies, each of which in the disclosed em- use initiators common to processes of the art. bodiment is a multilayered assembly including Electron beam polymerized gels afford the an electrode layer of electrically conductive desired advantages of being ultra thin and material and a layer of thermally conductive having a high electrophoretic resolution with material which serves as a heat sink. These elec- programmable porosity profiles. trode assemblies are adjustably supported in spaced-apart but confronting relationship to one another with the gel and support sheet sand- wiched therebetween. At the same time, the elec- trode layers are connected to a source of power 4840784 for producing an electric field across the gel and support sheet in order to transfer the specimen from the gel to the sheet by means ofelectroblot- U S E O F P Y R Y L I U M A N D ting. In the specific embodiment disclosed, the T H I A P Y R Y L I U M C O M P O U N D S spacing between the electrode assemblies can be AS B I O L O G I C A L S T A I N S adjustably decreased at a number of different points in order to ensure that they remain para- David S Frank, Robert Belly assigned to East- llel to one another in their adjusted positions man Kodak Company whereby to squeeze the gel and support sheet uniformly along the extend of the gel. Disclosed herein is an improvement in a method

for distinguishing cells in a biological sample by 4840730 staining with a dye, wherein the improvement

comprises employing as the dye a compound of C H R O M A T O G R A P H Y S Y S T E M the formula See Patent for Chemical Structure

U S I N G H O R I Z O N T A L F L O W wherein G is O or S; R1, R3, and R5 are indepen- dently selected from the group consisting of

C O L U M N S hydrogen, alkyl, aryl, aralkyl, amino, styryl, bi- s(diaryl)vinylene, and See Patent for Chemical

Vini Saxena assigned to Sepragen Corporation Structure wherein R is hydrogen or alkyl; Z represents the elements necessary to complete a

A system for chromatography using horizontal basic heterocyclic ring system of thetype used in (radial) flow columns is disclosed. The system cyanine dyes; n is 0 or 1; R2 is hydrogen or, taken provides rapid separation of complex biological together with either R 1 or R3, represents the ele- or organic mixtures at low operating pressures, ments needed to complete an aromatic or a car- The system may be used in a variety of applica- bocyclic ring system; R4 is hydrogen or, taken tions, such as in the analysis of the constituents together with either R3 or R5, represents the ele- of a mixture, or in large pilot or plant scale ments needed to complete an aromatic or a car- operations where the isolation of one or more bocyclic ring system; and X- is an anion. purified components of a mixture or fluid is re- quired for further testing or for commercial pur- poses. The system is designed to accommodate separation via a variety of routes including ion- exchange, affinity, hydrophobic, chiral, hydro- xylapatite, reverse phased as well as rapid 4840892 desalting techniques. P O L Y N U C L E O T I D E

H Y B R I D I Z A T I O N P R O B E S 4840756

Craig W Adams, Jeffry J Leary, Martin Rosen- R A D I A T I O N P R O C E S S F O R berg assigned to Smithkline Beckman Corpora-

P R E P A R A T I O N O F tion E L E C T R O P H O R E S l S G E L

M A T E R I A L Polynucleotide hybridization probes are labeled in non-probe regions but not in probe regions by

Richard C Ebersole, Robert P Foss assigned to E selectively protecting probe regions prior to I Du Pont Nemours and Company modification of the polynucleotide.