PATENT ABSTRACTS 371

Novel compositions and methods employing the compositions are provided involving eukaryotic plasmids having a replication system or replicon from an extrachromosomal element, a chromosomal replicator, and the regulatory signals of the structural gene for yeast 3- phosphoglycerokinase controlling expression of a structural gene. The plasmids find particular use in yeast for production of poly(amino acids).

4B66042

METHOD FOR THE DELIVERY OF GENETIC MATERIAL ACROSS

THE BLOOD BRAIN BARRIER

Edward Neuwelt

A method for treating genetic and acquired brain disorders is disclosed in which genetic material is introduced into the blood stream for delivery to the brain. Prior to delivery, the inter- endothelial structure of the BBB is chemicallv altered to permit passage of the genetic material therethrough. This is accomplished through os- motic disruption of the BBB by administration of suitable chemical agents. Prior to administra- tion, the genetic material can be inserted within the genome of a viral vector preferably incapable of replication in vivo. After crossing the blood brian barrier, the vector containing the genetic material enters the brain tissues where it delivers in a site-specific manner the genetic material in order to control adverse effects of the disease caused by defective genes. After delivery of the genetic material, the replication-defective character of the viral vector prevents its reproduction.

4866166

BIOASSAY FOR TRANSFORMING GENES AND GENES DETECTED

THEREBY

Michael Wigler assigned to Cold Spring Harbor Laboratory

A sensitive bioassay for transforming genes based on the tumorigenicity of cotransfected NIH3T3 cells in nude mice is disclosed. Genes mct2 and mcD have been detected in tissue breast cancer using this assay.

4866167

DETECTION OF HUMAN ORAL CELLS BY NUCLEIC ACID

HYBRIDIZATION

Michael C Chen, Pearl Chen, Lynn Klotz as- signed to Biotechnica Diagnostics Inc

A probe for the detection, in a sample obtained from the mouth of a human patient, of a micro- bial or human cell associated with a human oral medical disorder, the probe consisting essentially of a seament of DNA or RNA cauable of selec- tively hybridizing, under hybridizing conditions, to single-stranded DNA of the cell.

486795

LIVE ATTENUATED TEMPERATURE-SENSITIVE

AVIAN INFECTIOUS BRONCHITIS VIRUS VACCINES AND PREPARATION AND USE

THEREOF

Jack Gelb assigned to University of Delaware

The cold-adapted (ca) temperature-sensitive (ts) vaccine is prepared by serially passaging a suitably selected virus (e.g. Arkansas-type DPI strain) through a culture medium at a sub- optimal replication temperature which is less than about 35 degrees C., e.g. 28 degrees C., and harvesting the live, cold-adapted mutant for for- mulation into dosage units of, for example, ab- out 103 to about I05 EIDSO/bird. The ca ts mutant is much less able to replicate at 40 degrees-42 degrees C. (internal avian body tem- peratures) than at the temperature of the bird’s upper respiratory tract, which is below 40 degrees C. The vaccine can be administered by any of the usual techniques, including the spray technique. Vaccines of this invention appear to be very effective (immunogenic), but are also safe (non-pathogenic).

4868104

HOMOGENEOUS ASSAY FOR SPECIFIC POLYNUCLEOTIDES

Nurit Kurn, Chander Bahl, Edwin F Ullman as- signed to Syntex (U S A ) Inc