PATENT ABSTRACTS 843

4897354 4897471

MONOCLONAL ANTIBODY- SPECIFIC MEROZOITE

ANTIGENS

CONSENSUS HUMAN LEUKOCYTE INTERFERON

Wasim A Siddiqui assigned to University of Hawaii

Disclosed herein is a P. falciparum merozoite antigenic polypeptide of approximate molecular weight 185,000. The polypeptide and processing fragments are specific to, and isolable using, a monoclonal antibody produced by hybridoma cell line HB 9148. The polypeptides are useful in immunizing against malaria.

4897394

A N T I V I R A L C O M B I N A T I O N S

Thomas P Zimmerman, Gerald Wolberg as- signed to Burroughs Wellcome CO

This invention relates to synergistic combina- tions of nucleoside analogues, which are con- verted to viral DNA polymerase inhibitors through the action of at least one virus-induced enzyme, and nucleoside transport inhibitors, These combinations are especially useful in com- batting herpes virus infections.

Yitzhak Stabinsky assigned to Amgen

Described are rapid and highly efficient pro- cedures for the total synthesis of linear, double stranded DNA sequences in excess of about 200 base pairs in length, which sequences may com- prise entire structural genes. Novel sequences are prepared from two or more DNA subunits pro- vided with terminal regions comprising restric- tion endonuclease cleavage sites facilitating insertion of subunits into a selected vector for purposes of amplification during the course of the total assembly process~ The total, finally- assembled sequences include at least one, and preferably two or more, unique restriction endo- nuclease cleavage site(s) at intermediate posi- tions along the sequence, allowing for easy excision and replacement of subtmits and the correspondingly facile preparation of multiple structural analogs of polypeptides coded for by the sequences. Manufactured genes preferably' include codons selected from among alternative codons specifying the same amino acid on the basis of preferential expression in a projected host microorganism (e.g., E. coil) to be trans- formed. Illustrated is the preparation and ex- pression of manufactured genes capable of directing synthesis of human immune and leukocyte interferons and of other biologically active proteinaceous products, which products differ from naturally-occurring forms in terms of the identity and/or relative position of one or more amino acids, and in terms of one or more biological and pharmacological properties but which substantially retain other such properties.

4897466

HUMAN LYMPHOBLASTOID CELL LINE AND HYBRIDOMAS

DERIVED THEREFROM

James Larrick, Andrew R Raubitschek, Ken- neth Truitt assigned to Cetus Corporation

A 6-thioguanine-resistant subvariant of the EBV-transformed haman lymphoblastoid B cell line WI-L2 is described. The subvariant line, designated LTR228, fuses efficiently with human cells, Human*human hybridomas derived from LTR228 that produce monoclonal antibodies against tetanus toxin and blood group A are exemplified.

4898730

METHOD TO STIMULATE THE IMMUNE RESPONSE TO

SPECIFIC ANTIGENS

Julia G Levy, J Kevin Steele, Anthea Stammers, Vancouver, Canada assigned to The University of British Columbia

Factors secreted in the suppressor cascade are effective in contravening the suppression of the immune system in response to a particular anti- gen when properly administred. TsF2 factors may administered prior to or con- temporaneously with the antigen; TsFI factors,