PATENT ABSTRACTS

specific organisms, and further information can be gained by observing colorations, gas bubbles, precipitates and the like.

4906567

N O N - I M M U N O C H E M I C A L B I N D I N G O F

L I P O P O L Y S A C C H A R I D E S A N D S A N D W I C H A S S A Y S T H E R E F O R

Mark Connelly assigned to E I Dupont De Nemours and Company

Sandwich assays for detecting and identifying lipopolysaccharides of gram negative bacteria utilizing immobilized lipopolysaccharide bi- nding proteins and labelled detection reagents are provided. The active supports are also useful in removing LPS from biomedical and cosmetic preparations.

619

4906569

P R O C E S S F O R I S O L A T I N G A N D R E C O V E R I N G E R T H R I T O L F R O M

C U L T U R E M E D I U M C O N T A I N I N G T H E S A M E

Toshihiro Maeda, Isao Nakazawa, Takashi Kombu, Zama, Japan assigned to Mitsubishi Kasei Corporation; Nikken Chemicals Com- pany Limite

A process for readily isolating and recovering highly pure erythritol at a high crystallization yield from an erythritol-containing culture medium, which contains erythritol together with various impurities such as salts, coloring materials and polysaccharides, through chromatographic separation with the use of a strongly acidic cation exchange resin. The pro- cess of the present invention can be continuously operated, since the lowered separation capability of said cation exchange resin can be readily restored by treating the same with a warm alkali solution.

4906568

P R O C E S S F O R I S O L A T I O N O F C A R N I T I N E H Y D R O L Y A S E A N D

A N E F F E C T O R F R O M E N T E R O B A C T E R I A C E A E A N D U S E T H E R E O F T O P R E P A R E

C A R N I T I N E

Heinrich Jung, Kirsten Jung, Hans-Peter Kleber, Wolfen, German Democratic Republic assigned to Sigma Tau Industrie Farmaceutiche Riunite S p A

An enzyme carnitinehydrolyase is isolated from microorganisms belonging to the family En- terobacteriaceae, particularly Escherichia coli, Proteus vulgarius, Proteus mirabilis, Citrobacter freundii, Salmonella typhimurium, Salmonella anatum, e Salmonella cottbus, following anaerobic cultivation of the microorganisms in the presence of D,L-carnitine and/or crotonobetaine, by cell disruption and chromatography of the resulting protein extract. The enzyme, upon immobilization and activa- tion by an effector which is also obtained from the protein extract following enzyme isolation, is used for stereoselectively hydrating crotonobetaine into L(-)-carnitine by contacting a solution of crotonobetaine or crotonobetaine derivatives with carnitinehydrolyase in the pre- sence of the effector.

4906570

P O L Y M E R S B A S E D O N P O L Y V I N Y L E N E C A R B O N A T E

A N D / O R P O L Y H Y D R O X Y M E T H Y L E N E A S C A R R I E R S F O R B I O L O G I C A L L Y

A C T I V E S U B S T A N C E S

Otto Mauz, Liederbach, Federal Republic Of Germany assigned to Hoechst Aktiengesel- lschaft

Modified polymers based on polyvinylene car- bonate and/or polyhydroxymethylene are pre- pared containing covalently bonded units derived from particular alkoxylated compounds. Polymerization is carried out in the presence of the alkoxylated compounds and a particular dis- persion stabilizer. The modified polymer is suitable as a carrier for biologically active sub- stances or for affinity chromatography.

4906573

C U L T U R E M E D I U M F O R D E T E C T I O N O F B E E R S P O I L A G E

M I C R O O R G A N I S M S

Michael C Barney, Edward Kot, Etze Chicoye assigned to Miller Brewing Company

620 PATENT ABSTRACTS

Beer spoilage microorganisms are detected with a culture medium that is advantageous for detec- ting Lactobacillus spp., Pediococcus spp. and wild yeast, and which allows detection to be car- ried out in four days. The medium contains tomato juice, peptones, cysteine hydrochloride, sorbitan nonooleate, mono and disaccharides, yeast extract, beef extract, potassium acetate, malic acid, mono and dibasic potassium phos- phate, magnesium sulfate, maganese sulfate, sodium chloride, ferrous sulfate, beer, water and agar.

4906577

C E L L C U L T U R E B I O R E A C T O R

David W Armstrong, L Perr Fleming, Deborah G Grenzowski, Ottawa, Canada assigned to Canadian Patents and Development Ltd

concentrically to the inner wall surface of said tank structure and having a cylindrical space for accommodating a coolant; a plurality of fluid downcast pipes passed vertically through said space and being open at upper and lower ends thereof to upper and lower interior parts of the tank structure; means for passing the coolant through said space; and means for supplying fluid media into the tank structure, whereby, when the fluid media are supplied into the tank structure to a level above the upper ends of the fluid downcast pipes, the fluid media overflow into and descend through said pipes, while being cooled, into a lower interior part of the tank, while a portion of said fluid media in contact with the inner surface of the cooling jacket is also cooled and descends, the fluid media body as a whole thereby undergoing a natural circulatory flow due to convection.

4906715

There is disclosed a cell culture apparatus par- ticularly suitable for the culture of viable, shear sensitive cells, either grown in suspension or at- tached to a substrate such as microcarrier beads. The apparatus comprises a cell culture compart- ment and a compensation chamber disposed thereabove and serving to maintain sterile condi- tions for the cell culture compartment operating in a zero-headspace mode. The cell culture com- partment comprises a gas exchange tube connected to an outside source of gas and defining a downflow zone and a central upflow zone in the cell culture compartment. An Ar- chimedean screw-type auger is installed coax- ially in the cell culture compartment to provide a circulating flow of liquid therein. The turbulence of the flow is reduced by the provision of an up- per and lower flow guide surface. The gas ex- change tube is adapted to exchange gas from outside with the liquid inside the cell culture compartment both on its outer and on its inner surface thereby enhancing the efficiency of gas exchange.

N , N ' - D I V I N Y L A L K Y L U R E A C R O S S L I N K E D P O L Y M E R S , A

P R O C E S S F O R T H E I R P R E P A R A T I O N , A N D T H E I R U S E

Otto Mauz, Siegfried Noetzel, Bernhard Neumann, Liederbach, Federal Republic Of Germany assigned to Hoechst Aktiengesel- lsehaft

The invention relates to crosslinked polymers based on polyvinyl acylate or polyvinyl alcohol and having crosslinking agent units which are derived from crosslinking agents of the general formulae See Patent for Chemical Structure (I) and/or See Patent for Chemical Structure (II) These products, inter alia, are particularly hydrolysis-resistant and can have high cross- linking densities. The invention also relates to a process for preparing these polymers and to their use in chromatography and as carriers for bio- logically active substances.

4906578

A E R O B I C - C U L T I V A T I O N T A N K

Akira Suzuki, Hiroyoshi Konno, Shigeo Kono, Tokyo, Japan assigned to Kirin Beer Kabushiki Kaisha

An aerobic-cultivation tank of vertical type comprises: a closed outer tank structure of cylin- drical shape; an internal cooling jacket secured

4908307

H Y B R I D I Z A T I O N M E T H O D A N D P R O B E F O R D E T E C T I N G

N U C L E I C A C I D S E Q U E N C E S

Karin D Rodland, Peter J Russell assigned to Rodland Karin D

Improved hybridization probe compositions and method for detecting nucleic acids of interest