650 PATENT ABSTRACTS

was Constructed with fragments of the gene. Recombinant DNA clones producing specific antigenic determinants were isolated using monoclonal antibodies and the sequences of their insert DNAs were determined with a rapid primer extension method. Amino acid sequences for six different epitopes of the M. leprae protein were elucidated. A peptide containing sequences for one of these epitopes, which is unique to M. leprae, was synthesized and shown to bind the appropriate monoclonal antibody; The ap- proach described here can be used to elucidate rapidly protein epitopes that are recognized by antibodies or T cells. In addition, the well- characterized M. leprae antigens can be used in prevention, diagnosis and treatment of leprosy.

4908203

M E T H O D F O R I N D U C I N G H I V N E U T R A L I Z I N G A N T I B O D I E S U S I N G A N I N T E R N A L I M A G E

I D I O T O P E

George Thornton, Rancho Bernardo, Canada assigned to Johnson & Johnson

The present invention contemplates a method for inducing a HIV neutralizing immune response using an OKT4A antibody molecule homolog as an immunogen. Vaccines for prac- ticing an immunization method of this invention are also described.

4908204

R E V E R S I B L Y B L O C K E D P L A S M I N , T - P A H Y B R I D

F I B R I N O L Y T I C E N Z Y M E S A N D P H A R M A C E U T I C A L

C O M P O S I T I O N S A N D A N T I - T H R O M B O T I C U S E T H E R E O F

Jeffery H Robinson, Ian Dodd, Epsom, United Kingdom assigned to Beecham Group p I c

A fibrinolytically active hybrid protein which comprises one chain of a 2-chain protease linked to a chain of a different 2-chain protease, or to the same chain of the same protease, at least one of the chains in the hybrid protein being derived from a fibrinolytically active protease, such that the hybrid protein has a catalytic site essential for fibrinolytic activity which is optionally blocked by a removable blocking group.

4908305

C O M P E T I T I V E E L I S A F O R D E T E R M I N A T I O N O F

N E U T R A L I Z I N G I B D V A N T I B O D Y

David B Snyder assigned to The University of Maryland

A method for determining the level of infectious bursal disease virus IBDV neutralizing antibody in poultry comprising a labeled monoclonal anti- body R63 having the IBDV neutralizing cap- ability of the monoclonal antibody expressed by hybridoma cell line ATCC HB-9490. Mono- clonal antibody R63 is specific to all known IBDV strains and serotypes and competes only with itself and other antibodies recognizing the same neutralizing epitope present in poultry sera. In a competition assay, the increase in un- bound labeled monoclonal antibody is an indication of the level of IBDV neutralizing anti- body present in poultry sera.

4908306

H U M A N P A P I L L O M A V I R U S 56 N U C L E I C A C I D H Y B R I D I Z A T I O N

P R O B E S A N D M E T H O D S F O R E M P L O Y I N G T H E S A M E

Attila T Lorincz assigned to Life Technologies lnc

Nucleic acid hybridization probes for human papillomavirns types and particularly human papillomavirns type 56; and methods for em- ploying the same.

4908308

M E T H O D F O R D E T E C T I N G A N I M A L - I N F E C T I V E P R O T O Z O A IN V I T R O A N D A M E T H O D F O R

D E T E C T I N G A G E N T S W H I C H B L O C K T H E D I F F E R E N T I A T I O N

T H E R E O F

Ploeg Lex H T Van der, Suzanne H Giannini, Charles Cantor assigned to The Trustees of Columbia University in the City of New York

This invention concerns a method for iden- tifying in vitro an animal-infective form of a parasitic protozoan which comprises recovering total mRNA from the protozoan and detecting