BOO PATENT ABSTRACTS

4916122

3 ' - A Z I D O - 2 ' , 3 ' D I D E O X Y U R I D I N E A N T I - R E T R O V I R A L

C O M P O S I T I O N

Chung K Chu, Raymond F Schinazi assigned to University of Georgia Research Foundation Inc; Emory Universi

Compositions for the treatment of AIDS and ARC having the following compound as an ac- tive ingredient: See Patent for Chemical Struc- ture where RI is OH, monophosphate, diphosphate, or triphosphate; or a pharma- cologically acceptable salt thereof. The primary advantage of this compound is its highly selec- tive anti-retroviral activity, i.e., it significantly decreases viral replication as measured as reverse transcriptase activity while demonstrating orders of magnitude less cytotoxicity than other anti-viral compounds such as AZT. In a pre- ferred embodiment, the compound is present in an amount sufficient to inhibit the HIV reverse transcriptase activity but not significantly inhibit human DNA polymerase activity. Also included within the scope of this invention are 3'-azido- 2',3'-dideoxyuridine mono-, di-, and triphos- phate and compositions containing these compounds as the active agent.

4916212

D N A - S E Q U E N C E E N C O D I N G B I O S Y N T H E T I C I N S U L I N

P R E C U R S O R S A N D P R O C E S S F O R P R E P A R I N G T H E I N S U L I N

P R E C U R S O R S A N D H U M A N I N S U L I N

Arg connected to the amino terminus of the A(l- 21)-chain. Human insulin is prepared from the insulin precursors by in vitro conversion.

4917998

M E T H O D O F D E T E C T I N G A I D S V I R U S I N F E C T I O N

Denis R Burger, Andrew S Goldstein assigned to Epitope Inc

A method is disclosed for detecting the presence of HTLV III infected cells in a medium. The method comprises contacting the medium with monoclonal antibodies against an antigen pro- duced as a result of the infection and detecting the binding of the antibodies to the antigen. The antigen may be a gene product of the HTLV III virus or may be bound to such gene product. On the other hand the antigen may not be a viral gene product by may be produced as a result of the infection and may further be bound to a lymphocyte. The medium may be a human body fluid or a culture medium. A particular embodi- ment of the present method involves a method for determining the presence of an AIDS virus in a person, The method comprises combining a sample of a body fluid from the person with a monoclonal antibody that binds to an antigen produced as a result of the infection and detec- ting the binding of the monoclonal antibody to the antigen. The presence of the binding in- dicates the presence of an AIDS virus infection. Also disclosed are novel monoclonal antibodies, novel compositions of matter, and novel dia- gnostic kits.

Ja Markussen, Niels Fiil, Mogens Hansen, Kjel Norris, Gustav Ammerer, Lars Thim, Hans O Voigt, Herlev, Denmark assigned to Novo lndustri A/S

Human insulin precursors containing the pep- tide chain B(I-29)-A(I-21) of human insulin and derivatives thereof with a bridging chain con- necting the carboxyl terminus of the B(I-29)- chain with the amino terminus of the A(I-21)- chain are preps/red by culturing a yeast host transformed with a replicable expression vehicle capable of expressing a DNA-sequence encoding the insulin precursor. The bridging chain is pre- ferably relatively short and contains preferably from 2 to 8 amino acid residues. The bridging chain must not contain two adjacent basic amino acid residues (Lys or Arg) and has one Lys or

4917999

O L I G O N U C L E O T I D E P R O B E S F O R D E T E C T I O N O F A L P H A -

A M Y L A S E G E N E S

Graham Byng, Karen L Wollweber assigned to Miles Inc

Methods and compositions are provided for detecting a gene encoding alphaamylase from a nucleic acid sample suspected of containing said gene. Said methods utilize oligonucleotide probes of consensus sequences deduced from highly conserved DNA sequences of various Bacillus species.