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ANNEXURE

Czapek Dox media Sod.nitrate 2.0g Di potassium hydrogen phosphate 1.0 g MgSO4 0.5g KCl 0.5g FeSO4 0.1g Sucrose 30.0g Agar 15.0g Distilled water up to 1000 ml Peptone Yeast extract dextrose agar media Peptone 2.0g Yeast extract 2.0g Dextrose 5.0g Agar 15.0g Distilled water up to 1000 ml Sabouraud Agar media Peptone 10.0g Maltose/Dextrose 40.0g Agar 15.0g Distilled water up To 1000 ml (pH -5.6) Preparation of broth medium RPMI-1640 medium 10.4 gm 3-(N-morpholino) propanesulfonic acid (MOPS) 34.53g Distilled water 400 ml (pH- 7.0 at 25 °C) Phosphate buffer saline (PBS) NaCl 8 g Na2HPO4 1.15 g KCl 0.2 g KH2HPO4 0.2 g Distiilled water 800 ml pH -7.4 (with HCl)

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