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5. (cytolytic reaction)
(, ) ,
1) (Hemolytic reaction)
() ( hemolysin:)
++ ->
2) (Bacteriolytic reaction) () ( bacteriolysin)
+ + ->
Gram phospholipid -- (Pfeiffer)
Gram
(bacteriolysin, hemolysin) 56 30
6. (complement fixation reaction)
- -
1
2
, influenza, virus , , Rickettsia, ,
.() 56 30
7. (Absorption reaction)
()
8. ()(Inhibition reaction)
2 1
anti-hCG Anti-hCG
hCG Latex
virus
influenza virus
9. *
(Immunoassay)
(RIA: Radioimmunoassay)
(ELISA: Enzyme-linked immunosorbent assay, EIA)
(: Immunofluorescence)
Immuno-Blot
1)
125I
-
hapten
insulin
hormone
,
,
(solid phase method) plastic
Bead
Bead
1966 ELISA (peroxidase, alkaline phosphatase, -D-galactosidase) ,
()
filter
( ) (fluorescent antibody)
(anti-human -globulin)
Immuno-Blot
()
SDS polyacrylamide
Nitrocellulose
1-
2-
,
(autoradiography), (, )
10.
Turbidimetry
Nephelometry
(Flowcytometry)
Turbidimetry, nephelometry
* Turbidimetry ()
/
Immunoglobulin, CRP, ASO, Complement
* Nephelometry ()
, ,
Beer(Lambert-Bouquers)
: 0.2 ~ 1.0 (1.0 )
A=abc=log(100/%T)=2-log%T A(absorbance): a: (molar absorptivity) b: light path c: %T:
(monochromatic radiation)
, , , ,
, ,
DNA Reticulocyte
. What is Flow Cytometer ?
Flow () + Cyto ()
() (Particle) (Cell)
(sensing area)
Signal
.
Flowcytometer []
+ Meter ()
Consist of the FCM
1. Fluidic System
(Laser )
laser
+ - Charged Plates
FSC detector
Fluorescence detector
Sorting Mechanism
Light signal
Electronic pulse
Channel Number
Data stored by comuter
ADC
Summary
Thank you! ^^
http://blog.goodbyecrutches.com/GoodbyeCrutches-blog/bid/128865/Top-5-Vacation-Destinations-while-on-Crutches