124 PATENT ABSTRACTS

5045465

F E R M E N T A T I O N P R O C E S S F O R C R E A T I N I N E I M I N O H Y D R O L A S E

Jefferson C Lievense assigned to Eastman Kodak Company

A fermentation process and improved aqueous nutrient medium are used for the production of urease-free creatinine iminohydrolase from an aerobic soil microorganism. In the process, an inoculam culture of the microorganism is trans- ferred into a production medium to generate microorganisms in which creatininc im- inohydrolase production has been induced, said nutrient medium containing a source of ammonia and after the ammonia is substantially exhausted, incrementally adding a solution of creatinine, controlling the pH by addition of alpha-ketoglutaric acid, and extracting urease- free creatinine iminohydrotase. An improved aqueous nutrient medium for use as the produc- tion medium is disclosed.

5045470

D E V I C E F O R S U B M E R G E D C U L T U R E O F T I S S U E C E L L S

Get Kloss, Kolbermoor, Federal Republic Of Germany assigned to Stawag

An apparatus for submerged culture of tissue cells in which gas is supplied to the culture medium in an upright sterilizable container through a rotating hollow shaft having a plurality of sets of radially extending blades from which the gas is discharged only along upper sur- faces of the blades within a cage formed by ver- tically spaced hollow baffle plates connected by hollow posts through which gas is supplied to the baffle plates and is charged into the nutrient medium through porous parts of the plates.

5045479

C O N T I N U O U S F L O W C O M P E T I T I V E A S S A Y W I T H

R E F E R E N C E S Y S T E M

Arnold L Newman, William Stanbro assigned to The Johns Hopkins University

The invention relates to a continuous flow com- petitive assay system for the detection and

measurement of chenlical am.l hiochcn/icai analytes. It is a time-based, continuous on-linc measurement of analyte concentrations t2ont prising three functional assemblies connccted hi series: a sampler, a reactor and a detector Tag- ged immunochemical discharged from thcrcac tor is detected in the detector which contain~ ~l model of system response with Hlc tagged immunochemical and keeps lrack of {t~c amotmt of tagged immunochemical lost during Ihc course of the operation of the reactor

5051354

D E T E C T I O N O F A L T E R E D IGA1 IN F L U I D S A M P L E S

Jerry G Henslee, G Michael Hass, Jay R Schenck, Harry Rittenhouse assigned to Abbott Laboratories

Thc invention is a novel immunoassay method for detection of the immunoglobulin IgAl with O-linked oligosaccharides lacking sialic acid in fluid samples, such as blood, serum and plasma. Particuarly, the invention is an immunoassay method which extracts total IgA from fluid sam- ples by reaction with an immobilized anti-lgA antibody, and then detects only the lgAI frac- tion with labeled peanut agglutinin.

5051359

M E T H O D O F D E T E R M I N I N G T H E Q U A L I T Y O F A M E D I U M

William G Characklis assigned to Research and Development Institution Inc at Montana State University

An in situ method of determining the quality of a medium capable of supporting celhdar growth comprises obtaining viable cells which are anchored to a substrate, determining the value of at least one characteristic of the cell which is pro- portional by a factor to the number of the cells present under conditions which preserve cell vi- ability, calculating the number of cells present by multiplying the value of the characteristic by the factor, exposing the cells to the medium by in situ immersing the substrate with the anchored cells in the medium for a period of time and under conditions which preserve the viability of the cells and permit cell growth, withdrawiing the cells from the medium, repeating the steps for determining the value of the characteristic of the cells and for calculating the number of cells pre-