300 PATENT ABSTRACTS

A bioconversion reactor for the anaerobic fer- mentation of organic material. The bioconver- sion reactor comprises a shell enclosing a predetermined volume, an inlet port through which a liquid stream containing organic materials enters the shell, and an outlet port through which the stream exits the shell. A series of vertical and spaced-apart baffles are positioned within the shell to force the stream to flow under and over them as it passes from the inlet to the outlet port. The baffles present a bar- rier to the microorganisms within the shell causing them to rise and fall within the reactor but to move horizontally at a very slow rate. Treatment detention times of one day or less are possible.

5091316

BIOLOGICAL SAMPLE COLLECTION AND TRANSPORT

DEVICE

James F Monthony, David T Stitt, C Michael Gosnell, Shannon D Stewart assigned to Becton Dickinson and Company

A microbiological culture collection and trans- port device maintains viable organisms for periods of time longer than possible with existing sampling devices. It also allows recovery of detectable antigen at levels not achievable with conventional swabs. The new device has a sterile swabbing tip made with a non-toxic poly- urethane foam having open cells at its exposed surface. It does not require a transport medium and can be used dry. The device may further in- clude a sample inoculator to distribute or- ganisms collected onto a solid or semi solid medium. Methods for collecting and trans- porting microbiological specimens and for recovering detectable antigen are also described.

5091542

TRIS-MALEIMIDO COMPOUNDS AS INTERMEDIATES IN

TRIFUNCTIONAL ANTIBODY SYNTHESIS

Clarence Ahlem, Ann E Huang assigned to Hybritech Incorporated

The present invention is directed to a compound for use as an intermediate in the production of novel trifunctional antibody-like compounds.

More particularly, the present invention is direc- ted to a compound of the formula: See Pntent,for Chemical Structure wherein X is See Patent for Chemical Smcrure (Z)k(Z)k(Z)k, or (Z)kf701 +A,2 +BN.O +US,5 +SB,lI (Z)+HD k+A.3 +BL,A +BN,55 +SD,lI fS.5 (Z)+HD kfUS.36 +L ;+RE +RE+PS +RE+PS +RE+PS wherein k+32 I or 0; +RE+PO wherein Z is +RI +TL,4 +RB +AB,4 +M,l +AD,OO+MR,I CNH+AB.4

+AB,4 +M.I +AD,O O+MR,I C+AB, 4 b+AB.4 , +AB,4 O+AB.4 +M,l +AD,O O+MR,I C+AB,4 ,+RE +0 fRB +AB,4 NH+AB,4 +M,I +AD,OO+MR,I C+AB,4

+AB,4 O+AB,4 , I+RE

or +AB,4 NH+AB,4 +RE+PS +RE+PS +RE+PO

wherein s+32 I or0; +PG,2 +RE+POwherein n+32 I or 0; +RE+PO wherein q+32 I or 0: +RE+PO wherein Y is +Rl +TL,4 +RB +AB,4 SiAB, 4 S+AB,4 , +AB,4 +M,I +AD,O O+MR,I C+AB,4 NH+AB,4 fAB.4 NH+AB,4 +M,I +AD,O O+MR,i C+AB,4 , +AB,4 +M,l fAD.0 O+MR.l C+AB,4 O+AB,4 ,+RE +0 +RB +AB,4 O+AB,4 +M,l +AD,O O+MR,I C+AB,4, +AB,4 O+AB,4 +0 or +AB,4 NH+AB,4 ; +RE +RE+PS +RE+PS +RE+POwherein Y+40, is +Rl tTL.4 +RB +AB,4 S+AB,4 S+AB,4 , +AB,4 +M,I +AD,O O+MR,I C+AB,4 NH+AB,4 , +AB,4 NH+AB,4 +M,l +AD, 0 O+MR,I C+AB,4, fAB.4 +M,l +AD,O O+MR,I CfAB.4 O+AB,4 ,+RE +0 +RB +AB,4 O+AB,4 +M,I +AD,OO+MR,I C+AB,4, +AB,4O+AB,4 +0 or +AB,4 NH+AB,4 ;+RE +RE+PS + RE+ PS + REf PO wherein p or m may be the same or different and are integers ranging from 0 to 20 with the proviso that when n+32 0, the sum ofm and p is an integer ranging from I to 20. whereas when n + 32 I, p and m are each an in- teger that is at least I and the sum of p and m is an integer ranging from 2 to 20; + RE+PO wherein R+ HU I + L is straight or branched chain lower alkyl having from I to 6 carbon atoms or lower alkoxy having from I + 14 6 car- bon atoms; and +RE+PO wherein R+HU 2 +L is hydrogen, phenyl, + I3 COOH, or straight or branched chain lower alkyl having from I + 14 6 carbon atoms. with the proviso that the lower alkyl moiety may be mono sub- stituted by +I3 NH+HD 2+L, +I3 OH, or + I3 COOH. + RE + PA The compound of the present invention is useful as a trivalent coupling agent for linking Fabf40 -like fragments to form both bifunctional and trifunctional antibody-like compounds. + REnds. + REike compounds. + REds. + REnds. + REnds. +RE.+REds.+RE+ REnds.+ RE.+REs. + REnds. + REnds. + REd- s. + REs. + RE. + REnds. + REds. + REd-

PATENT ABSTRACTS 301

s. + REs. + REike compounds. + REds. + REnds. + REnds. + RE. + REs. + RE. + REREtituted by + 13 NH+HD 2+L, +I3 OH, or +13 COOH. + RE+ PA The compound of the present inven- tion is useful as a trivalent coupling agent for linking Fab+40 -like fragments to form both bi- functional and trifunctional antibody-like com- pounds. + REnds. + REnds. + REnds. + REituted by + 13 NH + HD 2 + L , +13OH,or +13COOH. +RE+PAThecom- pound of the present invention is useful as a trivalent coupling agent for linking Fabf40 - like fragments to form both bifunctional and trifunctional antibody-like compounds. + RE

5993045

BIOLOGICALLY ACTIVE LIPIDS BINDING MEMBRANE

RECEPTORS

Mohammed Shoyab, George J Todaro assigned to Oncogen

Neutral lipids are provided characterized by bi- nding to phorboid and ingenoid receptors. These lipids are found in a wide variety of cellular sour- ces as well as milk and may be isolated by specific extraction and chromatographic procedures. Depending upon the source, the glycerides may be mono- or di-glycerides, wherein the total number of carbon atoms of the fatty acis is in the range of 18 to 26, so that the monoglyceride has a fatty acid of at least 18 carbon atoms, while the di-glyceride has a fatty acid of at least 14 carbon atoms. The long chain fatty acids have at least one site of olefinic unsaturation.

5093081

DRY-TYPE ANALYTICAL ELEMENT FOR IMMUNOASSAY

Yukio Sudo, Yoshihiro Ashihara, Toshikage Hiraoka, Isao Nishizono, Shigeki Kageyama, Tetsuji Tanimoto, Saitama, Japan assigned to Fuji Photo Film Co Ltd; Fujirebio Kabushiki Kais

A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to en- zyme immunoassay, which comprises, (A) a water-insoluble macromolecular substrate, and (B) an antibody, reacting with the ligand in the

sample, conjugated with an enzyme capable of acting on the above water-insoluble macro- molecular substrate. The above analytical sub- stance further comprises, (C) a macromolecular substance which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element. The invention can conduct a highly sensitive and simple enzyme immunoassay.

5993231

SUBSTRATE COMPOSITION FOR ALKALINE PHOSPHATASE AND

METHOD FOR ASSAY USING SAME

Randal A Hoke assigned to Becton Dickinson and Company

A composition contains levamisole and a subs- trate for alkaline phosphatase in a high pH buf- fer including 2-amino-2-methyl-1-propanol. The composition may be included in a kit of materials useful in performing an immunoassay in which intestinal alkaline phosphatase is the label. The invention includes a method for detec- tion of a viral antigen by an immunoassay in- cluding intestinal alkaline phosphatase as the label and the composition of the invention to provide stabilized levamisole to inhibit non- intestinal alkaline phosphatase.

5093237

METHOD AND REAGENT FOR DETERMINING THE

BIOLOGICAL ACTIVITY OF ANTITHROMBIN III BY

MEASURING COAGULATION TIME

Masayasu Enomoto, Takatsuki, Japan assigned to Nippon Shoji Kabushiki Kaisha

A method for determination of biological ac- tivity of AT III by measuring coagulating time of blood plasma comprises mixing a specimen having AT III free-extrinsic coagulation factor- containing plasma, heparin and a prothrombin time-measuring reagent or a factor X-activating reagent and then measuring the coagulation time, and a reagent and plasma to be used there- for.