58
5.1 Proteomics tools on ExPASy

5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Embed Size (px)

Citation preview

Page 1: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.1 Proteomics tools on ExPASy

Page 2: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.2 (Part 1) Primary, secondary, and tertiary protein structure

Page 3: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.2 (Part 2) Primary, secondary, and tertiary protein structure

Page 4: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.3 (Part 1) Protein structural profiles

Page 5: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.3 (Part 2) Protein structural profiles

Page 6: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.3 (Part 3) Protein structural profiles

Page 7: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.4 (Part 1) Two-dimensional polyacrylamide gel electrophoresis

Page 8: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.4 (Part 2) Two-dimensional polyacrylamide gel electrophoresis

Page 9: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.5 Affinity chromatography

Page 10: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.6 (Part 1) Basis of mass spectrometry peptide sequencing

Page 11: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.6 (Part 2) Basis of mass spectrometry peptide sequencing

Page 12: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.6 (Part 3) Basis of mass spectrometry peptide sequencing

Page 13: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.7 (Part 1) Peptide sequencing by tandem mass spectrometry

Page 14: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.7 (Part 2) Peptide sequencing by tandem mass spectrometry

Page 15: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.8 (Part 1) Quantitative proteomics using ICAT reagents

Page 16: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.8 (Part 2) Quantitative proteomics using ICAT reagents

Page 17: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.8 (Part 3) Quantitative proteomics using ICAT reagents

Page 18: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.8 (Part 4) Quantitative proteomics using ICAT reagents

Page 19: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.9 (Part 1) Antibodies and immunohistochemistry

Page 20: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.9 (Part 2) Antibodies and immunohistochemistry

Page 21: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.10 Protein microarrays

Page 22: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.11 Self-assembling NAPPA protein microarrays

Page 23: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.12 (Part 1) Yeast two-hybrid (Y2H) screens

Page 24: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.12 (Part 2) Yeast two-hybrid (Y2H) screens

Page 25: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.13 Applications of structural proteomics

Page 26: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.14 Flow diagram for solving a protein structure

Page 27: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.15 The Drosophila Engrailed homeodomain dimer bound to DNA

Page 28: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.16 (Part 1) Fold recognition

Page 29: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.16 (Part 2) Fold recognition

Page 30: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.17 Frequency of mutations recovered in “saturation” screens

Page 31: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.18 (Part 1) The three major types of mutagen

Page 32: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.18 (Part 2) The three major types of mutagen

Page 33: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.18 (Part 3) The three major types of mutagen

Page 34: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.19 (Part 1) F1 and F3 genetic screens

Page 35: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.19 (Part 2) F1 and F3 genetic screens

Page 36: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.20 (Part 1) Targeted mutagenesis

Page 37: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.20 (Part 2) Targeted mutagenesis

Page 38: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.21 (Part 1) Inhibitory RNA expression (RNAi)

Page 39: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.21 (Part 2) Inhibitory RNA expression (RNAi)

Page 40: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.22 Targeted loss- and gain-of-function genetics

Page 41: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.23 Deficiency mapping

Page 42: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.24 Transposon mutagenesis and enhancer trap screens

Page 43: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.25 Floxing mice

Page 44: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

5.26 Genetic fingerprinting

Page 45: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Box 5.1 (Part 1) Hidden Markov Models in Domain Profiling

Page 46: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Box 5.1 (Part 2) Hidden Markov Models in Domain Profiling

Page 47: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Box 5.2 (Part 1) Biological Networks In Genome Science

Page 48: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Box 5.2 (Part 2) Biological Networks In Genome Science

Page 49: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Box 5.2 (Part 3) Biological Networks In Genome Science

Page 50: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Box 5.2 (Part 4) Biological Networks In Genome Science

Page 51: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Box 5.3 (Part 1) Transgenic Animals and Plants

Page 52: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Box 5.3 (Part 2) Transgenic Animals and Plants

Page 53: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Exercise 5.1 Structural annotation of a protein

Page 54: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Exercise 5.2 (Part 1) Identification of a protein on the basis of a MS profile

Page 55: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Exercise 5.2 (Part 2) Identification of a protein on the basis of a MS profile

Page 56: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Exercise 5.3 Formulating a network of protein interactions

Page 57: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

Exercise 5.4 Designing a genetic screen

Page 58: 5.1 Proteomics tools on ExPASy. 5.2 (Part 1) Primary, secondary, and tertiary protein structure