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6-FAMfor Proligo® Reagents

Product Information

• 6-FAM consists of one isomer derived from6-carboxyfluorescein

• Couples to the 5’-end of the oligonucleotide using standard synthesis protocols

• Protectinggroupsonthefluorescein moiety are removed under standard conditions of cleavage and deprotection with concentrated ammonia

• A detritylation step is not required since thefluoresceinphosphoramiditedoes not contain a DMT-group

SAFC Supply Solutions® is extending its Proligo® Reagents offer with 6-FAMasacommonfluorescentderivatizationreagentforcovalentlabeling of synthetic oligonucleotides.

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6

7

Test 6-FAM

HPLC Purity ≥97.0%31P-NMR ≥97.0%

Analyticalspecificationsof6-FAMOrdering Information

Description Unit Cat. No.

Compatible with ExpediteTM and Polygen® Instruments

6-FAM 1 x 0.1g X010181-01

Compatible with ABI® Instruments

6-FAM 1 x 0.1g X010131-01

For other quantities and custom packaging requirements please contact your sales representative to inquire. Some packaging options may not be available in your region.

Figure A. 6-FAM

Key Features of 6-FAM

• The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia. Standard procedures can be used if additional purificationisrequired.

• Lot to lot consistent purity and performance

• Manufacturedunderacertified ISO 9000:2000 quality system

Sigma-Aldrich Biochemie GmbHGeorg-Heyken-Strasse 1421147Hamburg,Germany

+494079702250(Europe)+18777765446(USTollFree)

Global Email: safcproligoreagents@sial.com