Slide #1

6850/6890 Splitless Animation - Split/Splitless Inlet - Component Identification

Septum

Liner

Liner O-Ring

Gold Seal

Capillary Column

9/27/02

Slide #2

6850/90 Split/Splitless Inlet - Component Identification

SeptumSEPTUM PURGE

VENT

Split Vent

Slide #3

6850/90 Splitless Animation - Default Conditions

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

PURGEVALVEOPEN

TOTAL FLOWCONTROL LOOP

SPLIT

VENT

GOLD SEAL

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI0

ML/MIN

SPLIT VENTTRAP

0

PSI

Slide #4

6850/90 Splitless Animation - Total Flow Setting

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

PURGEVALVEOPEN

TOTAL FLOWCONTROL LOOP

SPLIT

VENT

55 ML/MIN

GOLD SEAL

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI

PRIMARY FLOW PATH

REDUCED FLOW PATH

55

ML/MIN

SPLIT VENTTRAP

0

PSI

Slide #5

6850/90 Splitless Animation - Inlet Pressure Setting

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVEOPEN

TOTAL FLOWCONTROL LOOP

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI

PRIMARY FLOW PATH

REDUCED FLOW PATH

55

ML/MIN

SPLIT VENTTRAP

10

PSI

SPLIT

VENT

50 ML/MIN

COLUMN FLOW

2 ML/MINPRESSURIZED GAS

BACK PRESSURE CONTROL LOOP

Mode: SplitlessTemp 250 250Pressure 10.0 10.0Purge Time 0.50Purge Flow 50.0Tot Flow 55.0Gas Saver OFF

Slide #6

6890 Splitless Animation - GC Status before Pre-Run

Slide #7

6890 Splitless Animation - GC Status after Pre-Run

Slide #8

6850/90 Splitless Animation - Injection Prep - Run

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVE

CLOSED

FORWARD PRESSURECONTROL LOOP

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI

PRIMARY FLOW PATH

REDUCED FLOW PATH

5

ML/MIN

SPLIT VENTTRAP

10

PSI

SPLIT

VENT

0 ML/MIN

COLUMN FLOW

2 ML/MINPRESSURIZED GAS

Mode: SplitlessTemp 250 250Pressure 10.0 10.0Purge Time 0.50Purge Flow 50.0Tot Flow 5.0Gas Saver OFF

Slide #9

6850/90 Splitless Prep Run - Ready Animation

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVE

CLOSED

FORWARD PRESSURECONTROL LOOP

GOLD SEAL

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI

PRIMARY FLOW PATH

REDUCED FLOW PATH

5

ML/MIN

SPLIT VENTTRAP

10

PSI

SPLIT

VENT

0 ML/MIN

COLUMN FLOW

2 ML/MINPRESSURIZED GAS

Slide #10

6850/90 Splitless Begin Injection Animation

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVE

CLOSED

FORWARD PRESSURECONTROL LOOP

GOLD SEAL

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI5

ML/MIN

SPLIT VENTTRAP

10

PSI

SPLIT

VENT

0 ML/MIN

COLUMN FLOW

2 ML/MIN

Slide #11

6850/90 Splitless Inject Sample Animation

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVE

CLOSED

FORWARD PRESSURECONTROL LOOP

GOLD SEAL

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI5

ML/MIN

SPLIT VENTTRAP

10

PSI

SPLIT

VENT

0 ML/MIN

COLUMN FLOW

2 ML/MIN

Slide #12

6850/90 Splitless Sample Vaporizes Animation

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVE

CLOSED

FORWARD PRESSURECONTROL LOOP

GOLD SEAL

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI5

ML/MIN

SPLIT VENTTRAP

10

PSI

SPLIT

VENT

0 ML/MIN

COLUMN FLOW

2 ML/MIN

Slide #13

6850/90 Splitless Liner Capacity? Animation

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVE

CLOSED

FORWARD PRESSURECONTROL LOOP

GOLD SEAL

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI5

ML/MIN

SPLIT VENTTRAP

10

PSI

SPLIT

VENT

0 ML/MIN

COLUMN FLOW

2 ML/MIN

Larger O.D Linerto minimize sample

contact with inlet bodyTapered Liner tominimize sample contact

with Gold Seal

Slide #14

6850/90 Splitless Sample Transfer to Animation Column - Liner Volume vs. Column Flow

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVE

CLOSED

FORWARD PRESSURECONTROL LOOP

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI5

ML/MIN

SPLIT VENTTRAP

10

PSI

SPLIT

VENT

0 ML/MIN

COLUMN FLOW

2 ML/MIN

OVEN < BP OF SOLVENTSAMPLE RE-CONDENSES

AT HEAD OF COLUMN

FOR 1 ML LINER VOLUMEAND 2 ML/MIN COLUMN FLOW

PURGE DELAY SHOULD BE0.5 MINUTES MINIMUM

Slide #15

6850/90 Splitless Pulsed Splitless Mode - Animation Reduced Purge Time

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVE

CLOSED

FORWARD PRESSURECONTROL LOOP

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI7

ML/MIN

SPLIT VENTTRAP

20

PSI

SPLIT

VENT

0 ML/MIN

OVEN < BP OF SOLVENTSAMPLE RE-CONDENSES

AT HEAD OF COLUMN

Mode: Pulsed SplitlessTemp 250 250Pressure 10.0 10.0Pulse Press 20.0Pulse Time 0.25Purge Time 0.25Purge Flow 50.0Tot Flow 7.0Gas Saver OFF

COLUMN FLOW INCREASES TO 4 ML/MIN CUTTTING PURGE TIME IN HALF

Slide #16

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVE

CLOSED

FORWARD PRESSURECONTROL LOOP

GOLD SEAL

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI5

ML/MIN

SPLIT VENTTRAP

10

PSI

SPLIT

VENT

0 ML/MIN

COLUMN FLOW

2 ML/MIN

OVEN < BP OF SOLVENTSAMPLE RE-CONDENSES

AT HEAD OF COLUMN

6850/90 Splitless Sample Re-Condenses Animation At the Head of the Column

Slide #17

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVE

CLOSED

FORWARD PRESSURECONTROL LOOP

GOLD SEAL

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI5

ML/MIN

SPLIT VENTTRAP

10

PSI

SPLIT

VENT

0 ML/MIN

COLUMN FLOW

2 ML/MIN

OVEN < BP OF SOLVENTSAMPLE RE-CONDENSES

AT HEAD OF COLUMN

6850/90 Splitless Sample Transfer to Animation Column Continues...

Slide #18

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVE

CLOSED

FORWARD PRESSURECONTROL LOOP

GOLD SEAL

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI5

ML/MIN

SPLIT VENTTRAP

10

PSI

SPLIT

VENT

0 ML/MIN

COLUMN FLOW

2 ML/MIN

OVEN < BP OF SOLVENTSAMPLE RE-CONDENSES

AT HEAD OF COLUMN

6850/9090 Splitless Until the end of the Animation Splitless Purge Delay...

Slide #19

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVEOPEN

TOTAL FLOWCONTROL LOOP

GOLD SEAL

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI

PRIMARY FLOW PATH

REDUCED FLOW PATH

55

ML/MIN

SPLIT VENTTRAP

10

PSI

SPLIT

VENT

50 ML/MIN

COLUMN FLOW

2 ML/MINPRESSURIZED GAS

BACK PRESSURE CONTROL LOOP

6850/90 Splitless Animation - Inlet is Returned to Split Configuration - Residual Solvent is flushed from Inlet - GC Temperature Program and Separation Begins

Slide #20

6850/90 Splitless Press Fit Splitless Liner - Animation With Hole - No sample

Contact with gold seal

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVE

CLOSED

FORWARD PRESSURECONTROL LOOP

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI7

ML/MIN

SPLIT VENTTRAP

20

PSI

SPLIT

VENT

0 ML/MIN

OVEN < BP OF SOLVENTSAMPLE RE-CONDENSES

AT HEAD OF COLUMN

Mode: Pulsed SplitlessTemp 250 250Pressure 10.0 10.0Pulse Press 20.0Pulse Time 0.25Purge Time 0.25Purge Flow 50.0Tot Flow 7.0Gas Saver OFF

COLUMN FLOW INCREASES TO 4 ML/MIN CUTTTING PURGE TIME IN HALF

Slide #21

FLOWSENSOR

PROPORTIONALVALVE 1

PRESSURESENSOR

SEPTUM PURGEREGULATOR

FIXED @ 3ML/MIN

PURGEVALVEOPEN

TOTAL FLOWCONTROL LOOP

GOLD SEAL

INPUTPRESSURE

PROPORTIONALVALVE 2

80 PSI

PRIMARY FLOW PATH

REDUCED FLOW PATH

55

ML/MIN

SPLIT VENTTRAP

10

PSI

SPLIT

VENT

50 ML/MIN

COLUMN FLOW

2 ML/MINPRESSURIZED GAS

BACK PRESSURE CONTROL LOOP

6850/90 Splitless Animation - Inlet is Returned to Split Configuration - Residual Solvent is flushed from Inlet - through the hole in the liner…..

Slide #22

Splitless Liner Selections

Single Taperwith wool

Double Taper Single

TaperDouble Taper

Single Taper

NO wool

Direct Connect

Hole for EPC

Slide #23

6890 Splitless Chromatogram -

min0 1 2 3 4 5 6 7 8 9

pA

0

500

1000

1500

2000

FID1 A, (A:\FIDSPLS.D)

Area: 1.41189e+007

1.0

01

5.9

37

6.7

95

7.6

37