9. Primo Molecular Biology Plasticware€¦ · 9 Plastic for Molecular Biology 76 Primo® FrameStar® 480/96 for Roche LightCycler® 480 The FrameStar® 480/96 plates are semi-skirted

9. Primo® Molecular Biology Plasticware• • • •

Frame Star® PCR PlatesStandard PCR PlatesScreening PlatesStripsAdhesive SealingHeat SealingPCR Thin Wall TubesPrimo® Storage Plates

• • • •

9 Plastic for Molecular Biology

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Primo® Frame Star® PCR plates

FrameStar® 2-component plates improve consistency of PCR results

A special technology combines an extremely rigid structure (polycarbonate skirt and deck ) with thin wall tubes to guarantee optimal results in PCR experiments. The two-component design guarantees optimal mechanical stability and eliminates any distortion with the advantage to improve the integrity of the seal. Several studies have demonstrated that the high thermal stability of Frame Star®Plates compared to the one piece polypropylene plates eliminates any risk of sample evaporation indeed the excessive thermal expansion may have a damaging effect on seal integrity. Moreover the rigid structure of the plates is ideal for the use with robotic instruments such as stackers and liquid handling robots. Plates are moulded by injection of clear virgin polymer, the entire process is carried out in a clean room facility. Well integrity and absence of contaminants is 100% guaranteed, each single well is tested for leakage and contaminants such as human DNA (genomic DNA free), nucleases and PCR inhibitors.Plates have been tested with success on most automation platforms: Hamilton, Beckman, Tecan, ZymarK. EuroClone offers a complete portfolio of PCR plates with full skirted semi skirted and non skirted plates FrameStar® plates are available with purple, blue, red, green, clear and black frames, all with clear tubes. in addition we offer plates with white tubes optimised for fluorescence assays.Plates with black tubes are also available. Please contact [email protected] if the plate you require is not available.

FrameStar® PCR plates are covered by one or more of the following U.S. patents or their foreign counterparts, owned by Eppendorf AG: US Patent nos. 7,347,977 and 6,340,589

Fluorescence based Analysis For experiments involving fluorescence detection, e.g. Q-PCR, we have developed plates which are especially low in autofluorescence. For this type of experiment we recommend to use our plates with white wells and black frames: White wells have shown to maximise signal intensity due to the reflective properties of the pigment. At the same time cross talk between adjacent wells is minimised by the black, light absorbing plate frame and deck. This is a colour combination that cannot be offered by traditional one piece PCR plates.

* COMPATIBILITY CHART AND TECHNICAL DATA INFORMATION ARE AVAILABLE IN THE

TECHNICAL APPENDIX

Bar Coding

All skirted and semi-skirted plates are available with linear barcodes for identification and traceability. The labels are highly scratch resistant and can withstand cold storage (-80°C) and solvents, such as DMSO. Either single or double barcodes are available off-the-shelf and a variety of customisations are possible.

Manufacturing & Quality Control

FrameStar® plates are produced under clean-room conditions in the most modern injection moulding facilities. Particles, bacterial cells and other contaminants are filtered from the atmosphere so that FrameStar® plates are kept free from any factors which may affect the quality of experimental results. Only virgin polymers are used to avoid the presence of impurities. Our stringent QC procedures guarantee 100% well integrity and the absence of contaminants:

3 Each individual plate well is robotically tested for holes 3 Leak tests are performed to test the seal integrity 3 Biological test ensure the absence of human DNA, nucleases and other PCR inhibitors.

Plastic for Molecular Biology 9

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Primo® Framestar® 384 Well Skirted for robotic use

Designed for high-throughput PCR. FrameStar 384 is compatible with the majority of 384 block PCR, qPCR and sequencing instruments. The rigid two-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems.

Features Recommended for low volume PCR Polycarbonate frame for mechanical stability of the plate Polypropylene thin-walled tubes for optimum PCR results Improved seal integrity due to thermal stability of frame Certified free of human and bacterial DNA, RNases and DNasesIdeal for use with robotic systems Compatible with majority of 384 block PCR, qPCR and sequencing instruments Available with black plate and white wells for fluorescent based analysis Different colour optionsAlphanumeric grid-referenceAvailable barcodedAutoclavable

Cat. Description Colour frame Qty/CaseECPCR0384B Primo® Framestar® PCR plate 384 clear wells blue 50 ECPCR0384C Primo® Framestar® PCR plate 384 clear wells clear 50 ECPCR0384R Primo® Framestar® PCR plate 384 clear wells red 50 ECPCR0384BW Primo® Framestar® PCR plate 384 white wells SPECIAL x optical assays black 50


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Primo® Framestar® 480/384 for Roche LightCycler® 480

The dimensions of this plate are designed to optimise assay conditions on the Roche LightCycler® 480, 384 well for reaction volumes of 5-20μl. The rigid two-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems.

Features Compatible with the Roche LightCycler® 480 Recommended for low volume PCR Polycarbonate frame for mechanical stability of the plate Polypropylene thin-walled tubes for optimum PCR results Improved seal integrity due to thermal stability of frame Certified free of human and bacterial DNA, RNases and DNasesIdeal for use with robotic systems Alphanumeric grid-referenceAvailable barcodedAutoclavable

Cat. Description Colour frame Qty/CaseECPCR0380 Primo®FrameStar® 480, 384well PCR plate clear wells,clear frame 50ECPCR0381 Primo®FrameStar® 480, 384well PCR plate white wells,clear frame 50ECPCR0382 ECPCR0381 + qPCR adhesive seals (ECPCR0560) 50ECPCR0383 ECPCR0380 + qPCR adhesive seals (ECPCR0560) 50


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Primo® Framestar® 96 Well Skirted Low Profile

Low profile plates have shorter wells than standard profile plates, decreasing the “dead space” between the heated lid of the thermal cycler and the sample. This eliminates condensation on the side wall of the tube, preventing reduction in PCR volume and increasing the efficiency of the reaction. Low profile products are especially recommended for use with reaction volumes below 20μl. The rigid two-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems.

Features Recommended for low volume PCR Polycarbonate frame for mechanical stability of the plate Polypropylene thin-walled tubes for optimum PCR results Improved seal integrity due to thermal stability of frameCertified free of human and bacterial DNA, RNases and DNases Recommended for use with robotic systems Different colour options Available with black plate and white wells for fluorescent based analysis Available barcoded Autoclavable



TECHNICAL APPENDIX


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Primo® FrameStar® 480/96 for Roche LightCycler® 480

The FrameStar® 480/96 plates are semi-skirted and the dimensions are designed to achieve optimised assay conditions on the Roche LightCycler® 480, 96well for reaction volumes of 10-100μl. The rigid two-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems. The semi-skirt allows for labelling or barcoding.

Features Compatible with the Roche LightCycler® 480 Recommended for low volume PCR Polycarbonate frame for mechanical stability of the plate Polypropylene thin-walled tubes for optimum PCR results Improved seal integrity due to thermal stability of frame Ideal for use with robotic systems Available barcoded Autoclavable

Cat. Description Colour frame Qty/CaseECPCR0950 Primo®FrameStar® 480, 96well semi-skirted PCR plate clear wells, clear frame 50 ECPCR0951 Primo®FrameStar® 480, 96well semi-skirted PCR plate white wells, clear frame 50 ECPCR0952 ECPCR0951+ 50 qPCR adhesive seals (ECPCR0560) 50 ECPCR0953 ECPCR0950+ 50 qPCR adhesive seals (ECPCR0560) 50


TECHNICAL APPENDIX


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Primo® FrameStar® 96 Semi skirted with upstand

This semi-skirted plate is recommended for ABi thermal cyclers and sequencers; it can be used directly with the ABi instruments with no adapters necessary. The rigid two-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems. The semi-skirt allows for labelling or barcoding.

Features Ideal for ABi thermal cyclers & sequencers Recommended for low volume PCR Polycarbonate frame for mechanical stability of the plate Polypropylene thin-walled tubes for optimum PCR results Improved seal integrity due to thermal stability of frame Certified free of human and bacterial DNA, RNases and DNases Ideal for use with robotic systems Alphanumeric grid-referenceDifferent colour options Available with black plate and white wells for fluorescent based analysis Available barcoded Autoclavable



TECHNICAL APPENDIX


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Primo® FrameStar® Fast Plate 96 Semi skirted recommended for ABI Fast cyclers 50

This semi-skirted low profile plate is recommended for ABi Fast Block thermal cyclers. Low profile plates have shorter wells than standard profile plates, decreasing the “dead space” between the heated lid of the thermal cycler and the sample. This eliminates condensation on the side wall of the tube, preventing reduction in PCR volume and increasing the efficiency of the reaction. Low profile products are especially recommended for use with reaction volumes below 20μl. The rigid two-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems. The semi-skirt allows Tfor labelling or barcoding.

Features Compatible with the ABi Fast Block thermal cyclers Recommended for low volume PCR Polycarbonate frame for mechanical stability of the plate Polypropylene thin-walled tubes for optimum PCR resultsCertified free of human and bacterial DNA, RNases and DNases Improved seal integrity due to thermal stability of frame Ideal for use with robotic systems Alphanumeric grid-referenceAvailable barcoded Autoclavable

Cat. Description Qty/CaseECPCR0910C Primo® FrameStar® 96 PCRplates semi skirted recommended for ABI Fast cyclers 50ECPCR0910CW Primo® FrameStar® 96 PCRplates semi skirted recommended for ABI Fast cyclers, clear frame,white wells 50


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Primo® FrameStar® 96 Semi skirted Standard Profile (cut corner A12)

Specifically designed to be directly compatible with all major thermal cyclers including all ABi instruments, this plate can be used directly in ABi 96well instruments with no adapters necessary. The rigid two-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems. The semi-skirt allows for labelling or barcoding.

FeaturesPerfect fit for ABi thermal cyclers & sequencers Recommended for low volume PCR Polycarbonate frame for mechanical stability of the plate Polypropylene thin-walled tubes for optimum PCR resultsCertified free of human and bacterial DNA, RNases and DNases Improved seal integrity due to thermal stability of frameAlphanumeric grid-reference Ideal for use with robotic systems Available barcodedAutoclavable

Cat. Description Colour frame Qty/CaseECPCR0770C Primo® Framestar® PCR plate 96 clear wells clear 50 ECPCR0770BW Primo® Framestar® PCR plate 96 white wells SPECIAL x optical assays black 50


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Primo® FrameStar® 96, Universal semi-skirted plate (cut corner H1)

This universal semi-skirted plate is designed to fit all major thermal cyclers. The rigid two-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems. The semi-skirt allows for labelling or barcoding.

Features Recommended for low volume PCR Polycarbonate frame for mechanical stability of the plate Polypropylene thin-walled tubes for optimum PCR results Certified free of human and bacterial DNA, RNases and DNasesImproved seal integrity due to thermal stability of frame Alphanumeric grid-referenceIdeal for use with robotic systems Available barcoded Autoclavable

Cat. Description Colour frame Qty/CaseECPCR0900C Primo®FrameStar® 96well semi-skirted PCR plate clear wells, clear frame 50 ECPCR0901 Primo®FrameStar® 96well semi-skirted PCR plate white wells, clear frame 50


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Primo® FrameStar® 96 Non skirted Standard Profile

This standard profile 96well PCR plate is designed to fit most major thermal cyclers. The polycarbonate frame, within the 2-component design, reduces thermal expansion during PCR, ensuring the seal integrity remains intact, even at elevated temperatures.

FeaturesRecommended for low volume PCR Polycarbonate frame for mechanical stability of the plate Polypropylene thin-walled tubes for optimum PCR resultsCertified free of human and bacterial DNA, RNases and DNases Improved seal integrity due to thermal stability of frame Different colour optionsAlphanumeric grid-referenceAvailable with black plate and white wells for fluorescent based analysis Autoclavable

Cat. Description Colour frame Qty/CaseECPCR0710B PCR plate 96 clear wells blue 50 ECPCR0710C PCR plate 96 clear wells clear 50ECPCR0710R PCR plate 96 clear wells red 50ECPCR0710BW PCR plate 96 white wells SPECIAL x optical assays black 50


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Primo® FrameStar® 96 Non Skirted Low Profile

This low profile PCR plate has shorter wells than the standard profile plate (ECPCR0710), decreasing the “dead space” between the heated lid of the thermal cycler and the sample. This eliminates condensation on the side wall of the tube, preventing reduction in PCR volume and increasing the efficiency of the reaction. Low profile products are especially recommended for use with reaction volumes below 20μl. The polycarbonate frame, within the 2-component design, reduces thermal expansion during PCR, ensuring the seal integrity remains intact, even at elevated temperatures.

FeaturesRecommended for low volume PCR Polycarbonate frame for mechanical stability of the plate Polypropylene thin-walled tubes for optimum PCR results Certified free of human and bacterial DNA, RNases and DNasesImproved seal integrity due to thermal stability of frame Different colour optionsAlphanumeric grid-referenceIdeal for use with robotic systems Autoclavable

Cat. Description Colour frame Qty/CaseECPCR0720B Primo® FrameStar® PCR plate 96 clear wells low profile blue 50

ECPCR0720CPrimo® FrameStar® PCR plate 96 clear wells low profile

clear 50

ECPCR0720RPrimo® FrameStar® PCR plate 96 clear wells low profile

red 50

ECPCR0720BWPrimo® FrameStar® PCR plate 96 white wells low profileSPECIAL x optical assays

black 50


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Primo® Standard PCR platesEuroClone offers a range of PCR consumables for low to medium throughput applications. Our plates are moulded from virgin polypropylene under clean-room conditions and comply to the same stringent QC requirements as our Primo® FrameStar® range.

96well PCR Plate, Skirted, low profile

FeaturesLow profile design, 0.2ml maximum working volume (when used with sealing sheets)Cut-off corner for plate orientationAlphanumeric grid referenceStackableClear wells for improved sample visibilityRaised well rims to avoid cross contamination and to facilitate sealingCompatible with our domed and flat cap stripsHeat sealing and adhesive sealing options available

Cat. Description Colour frame Qty/CaseECPCR0740C Skirted PCR plate 96 clear wells clear 50

96well PCR Plate, Semi-Skirted

Features0.3ml maximum well volume (when used with adhesive and heat seals) Black grid reference for easy sample identification Cut-off corner for plate orientation Compatible with many major thermal cyclers Clear wells for improved sample visibility Heat sealing and adhesive sealing options available Compatible with our domed and flat cap strips Suitable for barcoding

Cat. Description Qty/CaseECPCR0760 96well PCR plate, semi skirted 50 plates

96well PCR Plate, Non-Skirted

Features Universal fit for all 0.2ml thermal cyclers and most sequencers Standard tube height with 0.ml working volume Black grid reference for easy sample identification Cut-off corner for plate orientation Clear wells for improved sample visibility Raised well rims to avoid cross contamination and to facilitate sealing Compatible with our domed and flat cap strip Heat sealing and adhesive sealing options available

Cat. Description Colour frame Qty/CaseECPCR0750C Non Skirted PCR plate 96 clear wells clear 50


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Primo® PCR Thin Wall Tubes Specifically designed for use in thermal transfer applications. The tube walls are ultra-thin and consistent so thermal transfer is precise. Our tubes are manufactured for 99% virgin polypropilene and are made from highly polished moulds and are free of lubricant, dye, and heavy metals or fillers.

Strips of 8 domed cap

FeaturesCompatible with our tube strips and 96well PCR platesEasy to applyLarge end tabs for easy removalLabelled for orientation

Cat.No. Description QtyECPCR0752 Strips of 8 domed cap 300

Primo® PCR tubes

Cat.No. Description Colour Qty/BagECPCR02F 0.2 ml, flat cap clear 1000 ECPCR02D 0.2 ml, domed cap clear 1000 ECPCR05F 0.5 ml, flat cap clear 1000

The difference is clearly amazing. Our PCR tubes are certified to be DNase, RNase and Pyrogen Free. The unique cap design guarantees a perfect fit which means there is no sample evaporation during thermal cycling.

Primo® Strips

Primo® PCR tubes and cap strip series

Cat.No. Description Colour Qty/BagECPCR0208 0.2 ml, 8 tubes/strip clear 125 ECPCR08D 0.2 ml, 8 domed cap strip clear 125

ECPCR08F0.2 ml, 8 flat cap/stripSPECIAL x QPCR

clear 125

ECPCR0208D 0.2 ml, 8 tubes/strip + domed caps clear 125 ECPCR0208F 0.2 ml, 8 tubes/strip + flat caps clear 125

Strips of 8 Flat Caps

FeaturesCompatible with our tube strips and 96well PCR platesOptically clear for fluorescence detection (e.g. Q-PCR)Easy to applyLarge end tabs for easy removalLabelled for orientation

Cat.No. Description QtyECPCR0751 Strips of 8 flat cap 300


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Primo® Adhesive Sealing EuroClone offers a wide range of adhesive sealing materials, including those with clear, pierceable, peelable and solvent resistant properties. Applications include PCR, QPCR, compound storage, low temperature storage and sample shipping. All seals are certified free from nucleases and human genomic DNA contamination.

Primo® PCR seal

A strong transparent adhesive seal recommended for PCR. Suitable for fluorescence applications.Available also in the piercable version with cross-cut, clear windows for sample retrieval with a tip or probe. The lack of adhesive under the cross cut section reduces the risk of the tip or probe getting clogged.

Features Strong transparent adhesive seal recommended for PCR Suitable for fluorescence applications. Seal integrity range -20°C to 110°C Peelable Non sterile

Cat. Description Dim.(mm) FormatECPCR0500 Primo® PCR seal 135x 80 100 sheets

ECPCR0566Primo® pierceablePCR seal

135x 80 100 sheets

Primo® qPCR seal

Optically clear film recommended for qPCR and other fluorescence applications.

Features Optically clear film recommended for qPCR Suitable for fluorescence applications. Seal integrity range -40°C to 110°C Peelable Non sterileNon pierceable

Cat. Description Dim.(mm) FormatECPCR0560 Primo®qPCR seal 135x 80 100 sheets

Q-Stick™ for qPCR (only for 96 well plates)

FeaturesStrong adhesive propertiesDiscrete optical windowsSuitable for fluorescence applications. Seal integrity range -20°C to 110°C Peelable Non sterileNon pierceable

Cat. Description Dim. Format (mm)ECPCR0565 Primo® Q-Stick 135x 80 100 sheets

Primo® PCR foil seal

Pierceable and peelable foil seal. When pierced the irregular manner in which the foil tears prevents the formation of a vacuum.

FeaturesSuitable for PCR and sample storageSeal integrity range -40°C to 110°C Peelable Non sterileNon pierceable

Cat.No Dim.(mm) QtyECPCR0550 135 x 80 100


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Primo® solvent resistant foil seal (for sample storage)

Peelable barrier film with a very high level of solvent resistance.

FeaturesSuitable for sealing microplates containing solvents including DMSOSeal integrity range -20°C to 80°C Non sterileNon pierceable

Cat. Description Dim. Format (mm)

ECPCR0512Primo® PCR solvent resistant foil seal

135x 80 100 sheets

Primo® microplate seal (for sample storage)

A medium strength transparent adhesive seal.

FeaturesSuitable for sample storage (aqueous).Seal integrity range -20°C to 80°CNon-sterile. Non-PierceablePeelable

Cat. Description Dim. Format (mm)ECPCR0510 Primo® microplate seal 135 x 80 100

ApplicationSample storage (aqueous)


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Primo® Heat sealingEuroClone offers a wide range of heating sealing materials, incuding those with clear, pierceable, peelable and solvent resistant properties. Applicationd include PCR, QPCR, compound storage, low temperature storage and sample shipping. We offer sealing rolls for use with all leading brand automated heat seals and cut sheets for use with manual sealer4s.All seal are certified free from nuclease and human genomic DNA contamination.

Primo® transparent seal I

FeaturesSuitable for qPCR, short term compound storagSeal integrity range -80°C to 80°C (110°C with pressurized PCR heated lidClear polymer film forming a peelable seal to PP, PS and COC platesGood optical claritySome solvent resistance

Cat.No Description Dim.(mm) QtyECPCR0541 Primo® Transparent Seal I 125 x 80 100 sheetsECPCR0540 Primo® Transparent Seal I 5000 x 78 1roll *ECPCR0542 Primo® Transparent Seal I 3500 x 115 1roll **

*= compatible with Thermo ALPS300™ and ALPS3000™**= compatible with Velocity 11 PlateLoc®

Primo® transparent seal II

FeaturesSuitable for PCR, especially water bath cyclers, Storage and disposal of hazardous materials, Sample inspectionSeal integrity range -80°C to 110°CWeld seal to PP that can not be peeled and is difficult to pierce. Good optical clarity and solvent resistance.Recommended for use with DMSO

Cat.No Description Dim.(mm) Qty

ECPCR0575Primo® Transparent Seal II

125 x 80 100 sheets

Primo® transparent seal for ABI 3730

FeaturesRecommended for use with the ABI 3730 Sequencer as the thinner structure pierces more easily.Seal integrity range -80°C to 80°C (110°C with pressurized PCR heated lid)PierceableNot peelableClear polymerSeals to PP, PS, PEGood optical clarity, some solvent resistance

Cat.No Description Dimension Qty

ECPCR0581Primo® Transparent Seal for ABI 3730

1000 perforated sheets 1 roll


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Primo® peelable seal

FeturesSuitable for low temperature, compound storage, short term room temperature compound storage, PCR.Seal integrity range -80°C to 80°CNon pierceablePeelable heat seal to PP plates. Can be peeled directly from freezerGood barrier to aqueous solutionsModerate resistance to solvents at room temperature

Cat.No Description Dim.(mm) QtyECPCR0521 Primo® Peelable Seal 125 x 80 100 sheetsECPCR0520 Primo® Peelable Seal 6000 x 78 1roll*ECPCR0522 Primo® Peelable Seal 5000 x 115 1roll**

ECPCR0587Primo® Peelable Seal DMSO resistant

125 x 80 100 sheets


5000 x 78 1roll*


5000 x 115 1roll**

*= compatible with Thermo ALPS300™ and ALPS3000™

**= compatible with Velocity 11 PlateLoc®

Primo® pierceable seal

FeaturesSuitable for PCR, compound storage, sample shipping. Foil based materialSeal integrity range -20°C to 120°CHeat seals to PP plates and can be easily pierced with a tipVery good solvent resistance including DMSOGood resealing onto existing seal allowing multiple access to samplesNot recommended for peeling

Cat.No Description Dim.(mm) QtyECPCR0531 Primo® Pierceable Seal 125 x 80 100 sheetsECPCR0530 Primo® Pierceable Seal 6100 x 78 1rollECPCR0532 Primo® Pierceable Seal 5000 x 115 1roll

*= compatible with Thermo ALPS300™ and ALPS3000™

**= compatible with Velocity 11 PlateLoc®

Primo® foil seal

FeaturesSuitable for Low temperature compound storage, short term room temperature compound storagePCRSeal integrity range -20°C to 110°CHeat seal to PP and PS plates that can be pierced with a tip or peeled off by hand. Good solvent resistance including DMSO. Resealing onto existing seal is possible.

Cat.No Description Dim.(mm) QtyECPCR0536 Primo® Foil seal 125 x 80 100 sheets

Primo® Thermal seal

FeaturesSuitable for Long term storage including storage and transportation at low temperature PCR, especially water bath cyclersSeal integrity range -200°C to 110°CSeals to PP with a very strong peelable sealNon pierceablePeelableHigh degree of sample protectionGood solvent resistance including DMSO

Cat.No Description Dim. (mm) QtyECPCR0591 Primo® Thermal Seal 125 x 80 100 sheets


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Primo® Semi-Automated Heat SealerThe semi-automated heat sealer is designed for low to medium throughput and is compatible with a wide range of sealing sheets and with plates of differing heights. Sealing time and temperature can be adjusted to allow for optimisation of sealing conditions to suit all plate and seal types. No damage to sample, plate and sealing sheet can be ensured. Plates and seals are manually loaded into the instrument but the sealing mechanism is automated to ensure that the same pressure is applied with every sealing process and that highest sealing consistency is achieved. The rapid heating element allows for quick start up of the unit within minutes. The temperature of the heating block is displayed in real time so that the heating up process can be monitored. When the unit is left idle it automatically switches into stand-by mode and the temperature of the heating element is reduced to 60 °C to save energy. The time at which the unit switches into stand-by mode, can be adjusted to suit the users needs. When the unit is left idle for longer periods it switches off automatically for added safety.

Adapters and Sealing Aids PRIMO® Heat sealer is a unique and cost saving adapter system. Deep-well plates and most other skirted plates can be sealed on the standard plate carrier (ECPCR0615, supplied with the unit). For all 96well PCR plates an additional plate carrier (ECPCR0625, optional extra) must be used. Both plate carriers can also be combined for easier handling of shallow well plates such as 384well PCR plates. For better sealing of those films which have a tendency to curl we offer sealing aids. A weighted platen (ECPCR0602) for use with deep-well blocks is provided with the unit and a sealing frame for use with all other plate designs is supplied when ordering the optional ECPCR0625 adapter.

Plate Formats The following plate formats can be used. • SBS standard polypropylene & polystyrene plates & deep-well blocks

(incl. 384well PCR plates), use adapter ECPCR0615 • 96well PCR plates (incl. skirted, semi-skirted and non-skirted formats),

use adapter ECPCR0625. The rapid heating element allows for a quick start up within minutes. The temperature is displayed in real time so that the heating up process can be monitored.

Features Automatic sealing process for unmatched sealing consistency Selectable temperature and time settings for easy optimisation of sealing conditions Single action operation – closing the draw initiates the process Rapid heating element for fast start-up Real time temperature display Auto stand-by function to save energy Auto switch-off function for added safety Works with all plate types Full range of thermal seals available.

Code Description

ECPCR0650 Heat sealer incl. universal power supply, power cord, manual, standard plate adapter (ECPCR0615) and seal platen (ECPCR0602)

AccessoriesECPCR0615 Adapter for deep-well and 384well plates ECPCR0616 Adapter for Roche 1536well PCR plate

ECPCR0602*Weighted seal platen to hold down sealing sheets that have a tendency to curl

ECPCR0625 Adapter for 96well PCR plates, includes sealing frame (ECPCR0612) ECPCR0612* Sealing frame for use with ECPCR0625 adapter

*These items are included with the Heat Sealer

Technical Data Weight: 9kg Dimensions: H 310 x D275 x W 181mm Voltage: 110/230v Power: 300W Sealing Temp. 120-200ºC

Primo® 96 Square-Well Deep-Well U-Bottom Microplate

EuroClone deep-well plates are produced in clean room facilities which are certified free of RNase, DNase, protease and endotoxin. We use only the highest grade polypropylene with resistance to corrosion from chemicals such as DMSO, phenol, and chloroform.The round-wells maximise sample retrieval yet minimise wicking (capillary action). Deep well plates are compatible with robotics and automation; bar-coding is available by arrangement.

Features

Round-wells maximise sample retrieval, but minimise wicking

Produced in clean-room facilities

Certified free of RNase, DNase, protease and endotoxin

Compatible with robotics and automation

Temperature range: -80°C to 121°C

Cat. Description Qty/case

ECPCR0136 2.2ml Primo®96 square-well, U-bottom deep-well plate, polypropylene, clear

50 plates

Documents

9. Primo Molecular Biology Plasticware€¦ · 9 Plastic for Molecular Biology 76 Primo® FrameStar® 480/96 for Roche LightCycler® 480 The FrameStar® 480/96 plates are semi-skirted