A. A. CORE MATERIAL A.1. FOUNDATION MODULaok.pte.hu/docs/th/file/2012/tantervek_2012/course_eng_biotechnol... · inherited and aquired mutations and consequent genetic diseases. The

UP MS Medical Biotechnology MSc major – course descriptions - academic year 2012/2013

A. A. CORE MATERIAL A.1. FOUNDATION MODUL 1. Title of course: BIOCHEMISTRY 2. Course coordinator: Dr. BALÁZS SÜMEGI, professor 3. Credits: 4 4. Weekly classes: 2+2 5. Prerequisits: - 6. Course desrciption: The subject covers the principles of chemical/biochemical processes occuring in living organisms. It deals with the fundamental metabolic processes and with the function, structure and regulation of the enzymes, transporters and other proteins which participate in the abovementioned processes. The subject uncovers the physical, chemical, thermodynamical and reaction-kinetical laws and rules of the essential metabolic processes and describes the structural characteristics of participating small molecules. It gives a deeper view into the fundamentals of structure-function relationships of small- and macromolecules, especially focusing on the structure, function and regulation of the molecules that are involved in the storage and transmission of genetic information, and dealing with basic molecular biology techniques. Furthermore, the regulation mechanisms of metabolic, hormonal and signal transduction pathways on the levels of the cell as well as of the organism are covered. The special biochemical characteristics of the different organs are also discussed. 7. Textbooks: Albert L. Lehninger-David L.Nelson-Michael M.Cox: Principles of Biochemistry 2nd edition, 1993 Worth Publishers, Inc. Biochemistry. Syllabus for Medical Students Edited by the Biochemical Departments of DOTE-POTE-SZOTE. Experimental Biochemistry Edited by Dénes Szabó, 1990 Recommended literature: Lubert Stryer: Biochemistry 4th edition, 1995 E.H.Freeman & Company, New York. Thomas M. Devlin: Textbook of Biochemistry. With Clinical Correlations 2nd edition John Wiley & Sons, New York 1. Title of course: INTRODUCTION TO MOLECULAR AND CELL BIOLOGY 2. Course coordinator: Dr. JÓZSEF SZEBERÉNYI, professor 3. Credits: 4 4. Weekly classes: 1+3 5. Prerequisits: - 6. Course desrciption: The subject covers the structure, functions and regulation of eukaryotic cells. The functional morphology of cell organelles (cell nucleus, endoplasmic reticulum, Golgi complex, mitochondria, lysosome, cytoskeleton, cell membrane) are discussed, together with the most important methods. Basic processes of molecular biology (the structure of genome and genes, DNA replication and repair, RNA transcription and processing, translation) and their regulation are also included in the curriculum. 7. Textbooks: Szeberényi J., Komáromy L.: Molecular Cell Biology Syllabus (handout) G.M. Cooper: The Cell. A Molecular Approach, Essential Cell Biology: An Introduction to the Molecular Biology of the Cell by Bruce Alberts, Ed: illustrated, Taylor & Francis, 1998,


Molecular Cell Biology by Harvey Lodish, Arnold Berk, S. Lawrence Zipursky Ed: 4, W.H. Freeman, 2001, Molecular Biology of the Cell: [media DVD-ROM Inside] by: Bruce Alberts, John Wilson, Ed: 5, Garland Science, 2008 1. Title of course: HUMAN PHYSIOLOGY 2. Course coordinator: Dr. LÁSZLÓ LÉNÁRD, professor 3. Credits: 4 4. Weekly classes: 2+2 5. Prerequisites: - 6. Course desrciption: The goal of the course is to disclose human physilogical processes to the students. By means of the knowledge of complex human physiological processes the biotechnology students will also learn the interconnections and mutual influences of physiological systems and they will utilize their knowledge in planning and executing related animal model experiments and pharmaceutical tests. 7. Textbooks: W.F. Ganong: Review of Medical Physiology Appleton and Lange, Lange Medical Publications, 1990-2006 A.C. Guyton: Textbook of Medical Physiology W.B. Saunders Company, 1996-2006 J.B. West (ed.): Best and Taylor's Physiological Basis of Medical Practice Williams and Wilkins, 1990-1998 Fonyó: Principles of Medical Physiology, Medicina Kiadó 2002 Physiology exercises Laboratory exercises in physiology 1st Semester 1. Title of course: GENETICS 2. Course coordinator: Dr BÉLA MELEGH, professor, 3. Credits: 4 4. Weekly classes: 2+2 5. Prerequisites: - 6. Course desrciption: The aim of human genetic studies to explain the human genome and inherited and aquired mutations and consequent genetic diseases. The course material involves classical elements of general genetics and specifics of human genetics. The course also discusses results from the Human Genome Project that was completed in 2003. The primary aim of the course is to highlight general characteristics of the human genome, modern diagnostic techniques, ways of prevention and treatment of diseases. The causes of genomic disorders, their pathomechanism, early identification of involved families, and human genetic methods are also covered. The curriculum follows the general principles of similar European and US courses, beginning at the foundation and finally reaching the most up-to-date results. 7. Kötelező és ajánlott irodalom: Lecture notes - Nelson Textbook of Paediatrics - Emery and Mueller: Elements of Medical Genetics - High-Yield Genetics, by Ronald W Dudek, John E Wiley, Lippincott Williams & Wilkins, 2008


1. Title of course: INFORMATICS 2. Course coordinator: Dr. GÁBOR PAULER, associate professor 3. Credits: 4 4. Weekly classes: 1+3 5. Prerequisites: - 6. Course desrciption: This course introduces students in the joint use of MS Access and MS Excel, with the pupose of enabling them to perform data processing and basic statistical analysis task in routine biotech research job. We stress on describing differences in purpose and capacities of spreadsheet and database software to avoid common misuses resulting data loss and redundant workload. We introduce students of very basics of programming in SQL, procedural VB, and cell functions coding. 7. Textbooks: PTE-PMMK Databases/Spreadheets, Data Mining 2 Electronic Course Material, Introduction to Bioinformatics by Arthur Lesk, Ed: 2nd, 2005, Oxford University Press, New York, Understanding Bioinformatics by Marketa Zvelebil and Jeremy O. Baum; R Programming for Bioinformatics (Chapman & Hall/CRC Computer Science & Data Analysis) by Robert Gentleman 1. Title of course: INTRODUCTION TO ECONOMICS 2. Course coordinator: Dr VARGA ATTILA, professor 3. Credits: 2 4. Weekly classes: 1+1 5. Prerequisits:- 6. Course desrciption: Aim of the module is to provide students with basic understanding of economics and with knowledge about economic processes. Economic analysis is applied throughout society, in business and finance but also in education, science and research. The course material will discuss the meaning of macroeconomics, as it influences biotechnology firms, startup and spinoff companies. For example, macroeconomics examines the economy as a whole to explain broad aggregates and their interactions. It will include national income and output, the unemployment rate, and price inflation and subaggregates like total consumption and investment spending and their components. Economic efficiency will also be described how well a system generates the maximum desired output with a given set of inputs and available technology. At the end of the module students will be able to analyse

1. Title of course: BIOPHYSICS 2. Course coordinator: Dr MIKLÓS NYITRAI , professor, 3. Credits: 4 4. Weekly classes: 2+2 5. Prerequisites: - 6. Course desrciption: The aim of the Biophysics curses is to introduce the students to the theoretical and experimental bases of the methods and applications commonly used in medical biotechnology. To achieve these aims we will provide detailed information in the lectures regarding the principals underlying these applications. The seminars and practicals will serve the aims of the manifestation of this knowledge in practical situations, during manual applications. An example for the subjects covered in biophysics. 7. Textbooks: 1. Medical Biophysics, (Ed.:Sándor Damjanovich, Judit Fidy, János Szöllősi); 2008, Medicina. 2. Biophysics practicals, PTE ÁOK; 3. Integrated Molecular and Cellular Biophysics by Valerica


economic phenomena and processes. Another aim of the module is to create additional basis for further studies and practice in the field of economics. The module offers effective help to understand economic changes, development and issues of governmental economic policies. Students will be able to analyse critically institutions of redistribution to and different social dilemmas. 7. Textbooks: P. Samuelson-W. Nordhaus (2005): Economics. McGraw-Hill International Edition. XVIII. Ed, Principles of Economics, 4th Edition by N. Gregory Mankiw , Principles of Microeconomics by N. Gregory Mankiw , Principles of Macroeconomics by N. Gregory Mankiw , Naked Economics: Undressing the Dismal Science by Charles Wheelan 1. Title of course: MANAGEMENT 2. Course coordinator: Dr. ÁKOS JARJABKA, associate professor 3. Credits: 2 4. Weekly classes: 1+1 5. Prerequisits:- 6. Course desrciption: The goal of this course: Gives a brief summary of themes and different methods of management science. In possession of management knowledge on basic level the students can define, analyse and solve the management and leading process and problems The course is included the most famous management theories, methods, processes and solutions. 7. Textbooks: 1. David Buchanan – Andrzej Huczynski: Organizational Behavior – An Introductory Text - , Prentice Hall – Financial Times, 5. edition, 2004., ISBN: 0 273 68222 9 2. Managing Small Business: An Entrepreneurial Emphasis by Carlos W. Moore, Justin Gooderl 1917- Longenecker, Ed: 14, Cengage Learning EMEA, 2008; The Management of Technological Innovation: Strategy and Practice by Mark Dodgson, David Gann, Ammon Salter, Ed: 2, Oxford University Press, 2008 1. Title of course: BUSINESS LAW 2. Course coordinator: Dr. TIBOR NOCHTA, associate professor, Head of Department 3. Credits: 1 4. Weekly classes: 1 5. Prerequisits: - 6. Course desrciption: The course will deal with the main issues of regulation of economy,businesse law, company law, contracts law, leas,-franchise factor - managemant consulting contracts, trade law. It will also give a general overview of insolvensy. 7. Textbooks: 1. Nochta Tibor: Company Law, 2. Nochta-Juhasz-Szécsényi: Law Regulation of Economy I-II. 2008 Budapest, 3. Nochta T. , Paths of Civil Law Laibility in Company Law; 4. K. Clarkson: Business law (West’s Business Law) Ed: 11th 1. Title of course: ETHICS IN BIOTECHNOLOGY 2. Course coordinator: Dr. JÁNOS KÁLLAI, professor 3. Credits: 1 4. Weekly classes: 1 5. Prerequisits:- 6. Course desrciption: The main aim of the course is to present the ethical considerations and dilemmas, which are related to the biotechnological researches and to the applications of biotechnology. Beyond that, during the course students will get acquainted with the social debates that are involved by the development of medical biotechnology, and also with the main elements of the European legal regulations in this area. During the semester the following subjects will be discussed: the ethics and the legal regulations of animal experiments, the ethical questions concerning organ and tissue transplantation, the ethical


issues of cloning and genetic interventions, the future prospects of biotechnology and their social implications. 7. Textbooks: - A Companion to Genethics, edited by Justin Burley and John Harris, Balckwell Publishers Ltd., Massachussets/Oxford, 2002. - Gehring, Verna V., Genetic Prospects: Essays On Biotechnology, Ethics, and Public Policy, Rowman & Littlefield Publishers, Inc., 2004. - Andrew Johnson, Alan Holland, Animal Biotechnology and Ethics, Springer, 1997. - Peter Singer, Practical Ethics, Cambridge University Press, 1979, 48-72. - Hoeyer, K., Koch, L. The ethics of functional genetics: same, same, but different?, Trends in Biotechnology, 24(9), 387-3789, 2006. - H. Verhoog, Naturalness and the genetic modification of animals, Trends in Biotechnology, 21(7), 294-297, 2003. - F. S. Collins, E. D. Green, et al., A vision for the future of genomic research, Nature, 422, 835-847, 2003 - Peter Singer, Practical Ethics, Cambridge University Press, 1979, 48-72. - Hoeyer, K., Koch, L. The ethics of functional genetics: same, same, but different?, Trends in Biotechnology, 24(9), 387-3789, 2006. - H. Verhoog, Naturalness and the genetic modification of animals, Trends in Biotechnology, 21(7), 294-297, 2003. - F. S. Collins, E. D. Green, et al., A vision for the future of genomic research, Nature, 422, 835-847, 2003. - A Companion to Genethics, edited by Justin Burley and John Harris, Balckwell Publishers Ltd., Massachussets/Oxford, 2002. - Gehring, Verna V., Genetic Prospects: Essays On Biotechnology, Ethics, and Public Policy, Rowman & Littlefield Publishers, Inc., 2004. - Andrew Johnson, Alan Holland, Animal Biotechnology and Ethics, Springer, 1997.


A.2. SPECIALIZED FOUNDATION MODUL 1. Title of course: NUCLEIC ACID MANIPULATION, VECTOR-DESIGN 2. Course coordinator: Dr. JUDIT PONGRÁCZ, associate professor 3. Credits: 4 4. Weekly classes: 1+3 5. Prerequisits: completed foundation modul 6. Course description: The aim of the course is to present the theoretical and practical background of nucleic acid manipulation and vector-design. The course discusses in detail the methods and aims of nucleic acid manipulation and also the establishment of recombinant viral vectors. Participants will become familiar with the practical utilization of vectors, the genetic manipulation of cells and laboratory animals, and how e.g. a gene-therapeutic intervention is designed in the case of a monogenic recessive hematological disorder. 7. Textbooks: 1. Principles of Gene Manipulation and Genomics, 7th Edition. S. B. Primrose & R. M. Twyman. Blackwell Publishing. 2006. 626 pages. ISBN 1 405 13544 1 2. Medical Biotechnology, editors: Pongracz J, Keen M, Elsevier (2009), 3. Human Molecular Genetics by Tom Strachan, Garland Publishing, (2004); 1. Title of course: PROTEINS AND PROTEIN NETWORKS 2. Course coordinator: Dr. FERENC KILÁR, professor 3. Credits: 4 4. Weekly classes: 1+3 5. Prerequisits: completed foundation modul 6. Course description: The purpose of this subject is to describe the protein networks that coordinates the cell-functions. Analysis of the proteome („phosphoproteome”, „immunoproteome”, „inflammaproteome”) with molecular biology, bioinformatics and biostatistical methods. Structure-function relationships in proteins. Protein associates. 7. Textbooks: D. Liu and F-Y. Wan, editors, Advances in Computational Intelligence: Theory & Applications, World Scientific, Hackensack, 2006. Panchenko, Anna; Przytycka, Teresa (Eds.), Protein-protein Interactions and Networks, Identification, Computer Analysis, and Prediction, Series: Computational Biology , Vol. 9, 2008, Springer, Petsko, Gregory A./ Ringe, Dagmar, Protein Structure and Function, Sinauer Associates Inc., 2004 Buxbaum, Engelbert, Fundamentals of Protein Structure and Function, 2007, Springer 1. Title of course: SIGNAL TRANSDUCTION 2. Course coordinator: Dr. TÍMEA BERKI, associate professor 3. Credits: 4 4. Weekly classes: 2+2 5. Prerequisits: completed foundation modul 6. Course description: Novel antibodies against receptors, protein kinase inhibitors, and antisense oligonucleotides targeting both signal transduction and gene expression will predominate the therapeutic approaches in the coming decades. The course focus on the therapeutic potential for targeting cell signaling mechanisms with particular attention to cancer therapies and inflammatory signaling pathways as well as immunomodulation. General concepts of inter-and intracellular signal transduction: receptor-mediated signal transduction, cell surface receptors, steroid hormone and nuclear receptors and their cytoplasmic signal transduction and nuclear responses, apoptotic cell signaling will be discussed together with the novel approaches to drug discovery in signal transduction.


Internationally known experts of the field guest lecturers from Hungarian universities are contributing in the course. 7. Textbooks: 1.Gerhard Krauss: Biochemistry of Signal Transduction and Regulation (Wiley) Finkel, Toren(ed.) ; 2. Gutkind, J. Silvio(ed.): Signal Transduction and Human Disease (John Wiley & Sons, Inc.), 3. Bioinformatics and Systems Biology: Collaborative Research and Resources, Frederick Marcus, Springer, 2008 1. Title of course: IMMUNOLOGY 2. Course coordinator: Dr. PÉTER NÉMETH, professor 3. Credits: 3 4. Weekly classes: 1+2 5. Prerequisits: Completed foundation modul 6. Course description: The subject comprises major immunological processes, their cellular and molecular components, discusses the regulation of a physiological immune response. The course also briefly summarizes basic processes of immune-pathologies (i.e. autoimmunity, tumor-immunity, etc). The practices allow for the participants to become familiar with standard and modern immunological techniques. 7. Textbooks: IMMUNOBIOLOGY 7 PB (Janeway's Immunobiology) (Immunobiology: The Immune System (Janeway)) by Kenneth M. Murphy, Paul Travers, and Mark Walport; Kuby Immunology (Kindt, Kuby Immunology) by Thomas J. Kindt, Barbara A. Osborne, and Richard A. Goldsby; Essentials of Clinical Immunology by H.Chapel, Ed:5, Blackwell Publishing, 2008 1. Title of course: MICROBIOLOGY 2. Course coordinator: Dr. JÚLIA SZEKERES-BARTHÓ, professor 3. Credits: 3 4. Weekly classes: 1+2 5. Prerequisits: Completed foundation modul 6. Course description: The subject explains the course of setting the diagnosis and performing an efficient antimicrobial treatment in the case or suspicion of an infectious disease. The course follows current trends based on modern biotechnological knowledge (i.e. increase in the significance of opportunistic pathogens, recognition of new pathogens, changes in the roles of conventional pathogens etc). 7. Textbooks: 1. Medical Microbiology and Immunology by Warren Levinson; 2. Microbial Biotechnology: Fundamentals of Applied Microbiology by Alexander N. Glazer and Hiroshi Nikaido; 3. Advances in Applied Microbiology by Wayne William Umbreit, Academic Press, 2005 1. Title of course: MOLECULAR BASIS OF MICROBIAL PATHOGENESIS 2. Course coordinator: Dr. CSABA FEKETE, associate professor 3. Credits: 3 4. Weekly classes: 1+2 5. Prerequisites: Completed foundation modul 6. Course desrciption: The course is concerned with modern aspects of molecular microbiology including examination of the regulation of the growth of bacteria and fungi, and the molecular determination of virulence in pathogenic microorganisms. The lectures will focus on current molecular and biochemical techniques that are the most frequently used in medical and clinical research. Theoretical and practical aspects of molecular techniques used in the prevention, diagnosis and treatment of major pathogens will also be discussed. We will talk about how these methods work, their pitfalls, and strengths. The lecture


component covers the molecular features of the interactions between pathogens and the host, including the role of the immune system and the use of antimicrobial drugs in combating infection. 7. Textbooks: Diagnostic Molecular Microbiology: Principles and Applications by David H. Persing, T. F. Smith, F. C. Tenover. Published by ASM Press, 1993. Medical Biomethods Handbook by John M. Walker, Ralph Rapley Contributor John M. Walker, Ralph Rapley Published by Humana Press, 2005. Fundamentals of Molecular Diagnostics. By David E. Bruns, Edward R. Ashwood, Carl A. Burtis, Barbara G. Sawyer, Published by Saunders Elsevier, 2007. Principles of Gene Manipulation and Genomics by S. Primrose and R. Twyman, Blackwell Publishing, 2006. Kiadott publikációk. 1. Title of course: MOLECULAR PATHOLOGY 2. Course coordinator: Dr. LÁSZLÓ PAJOR, professor 3. Credits: 2 4. Weekly classes: 2 5. Prerequisits: Completed foundation modul 6. Course description: Molecular aberrations of tumours best characterised at the molecular level, will be presented at oncopathology lectures. The main emphasis will be on – apart from chronic myeloproliferative disorders, acute myeloid and lymphoid leukaemias, B- and T-cell malignant lymphomas – breast cancer, brain tumours (gliomas), HPV associated cervical carcinoma, colorectal and pulmonary epithelial tumours, and childhood soft tissue cancers. The lecture will also address existing and potential target therapies, their molecular basis, and system of criteria for predictive pathological diagnostics. The practical part of the course involves laboratory presentations, and experiments with active student participation. The experiments involve convential and molecular cytogenetics, automated interphase cytogenetic, multiparametric flow cytometry and cell sorting, DNA and RNA PCR, quantitative PCR, allele discrimination quantitative PCR and mutation analysis. 7. Textbooks: Margaret A. Knowles and Peter J. Selby. Introduction to the Cellular and Molecular Biology of Cancer. Oxford University Press, USA; 4 edition. 2005. Michael Khan, Stella Pelengaris. The Molecular Biology of Cancer. Wiley-Blackwell; 1 edition. 2006., DeVita, Hellman, and Rosenberg's Cancer: Principles & Practice of Oncology by Vincent T. DeVita, Theodore S Lawrence, Steven A. Rosenberg, Robert A Weinberg, Ronald A DePinho, Ed: 8, Lippincott Williams & Wilkins, 2008 1. Title of course: DEVELOPMENTAL BIOLOGY 2. Course coordinator: Dr. PÉTER BALOGH, associate professor 3. Credits: 2 4. Weekly classes: 2 5. Prerequisits: completed foundation modul 6. Course description: This course covers the molecular and cellular bases of development in a variety of experimental organisms with special emphasis on mammalian and human models. With the observation of differential gene-activity and geneexpression patterns it explains the development of various tissues and organs. The course considers the possible pharmacological and pharmacotherapical applications in the biological development. 7. Textbooks: 1. Scott F. Gilbert: Developmental Biology 2006. Sinauer Associates, 2. Principles of Development by Lewis Wolpert, Jim Smith, Tom Jessell, and Peter Lawrence, Oxford University Press, Ed: 3rd, 3. Essential Developmental Biology by Jonathan M. W. Slack, Blackwell, 2006; 4. Evolutionary Developmental Biology by B.K. Hall, Kluwer, Ed 2nd 1. Title of course: IN VIVO TEST SYSTEMS AND TRANSGENIC ANIMALS 2. Course coordinator: Dr. PÉTER BALOGH, associate professor


3. Credits: 2 4. Weekly classes: 1+1 5. Prerequisits: completed foundation modul 6. Course description: The aim of this course is the presentation of current in vivo experimental approaches for the development and application of various genetically modified animals (mainly mice) in biomedical research. 7. Textbooks: Transgenic Animals: Generation and Use. Louis-Marie Houdebine CRC Press, 1997, Transgenic Animal Technology: A Laboratory Handbook by Carl A. Pinkert, Academic Press, Ed:2nd, 2002, What's Wrong with My Mouse?: Behavioral Phenotyping of Transgenic and Knockout Mice by J.N. Crawley, Ed:2nd, 2007; Mouse Genetics and Transgenics: A Practical Approach by Ian J. Jackson and Cathy Abbott, Oxford University Press, 2001 1. Title of course: TISSUE CULTURE TECHNIQUES 2. Course coordinator: Dr. JUDIT PONGRÁCZ, associate professor 3. Credits: 3 4. Weekly classes: 1+2 5. Prerequisits: completed foundation modul 6. Course description: The course material discusses the basic principles of cell culture techniques both for cell lines and primary tissues. Fundamental techniques for stem cell purification and stem cell culture will also be detailed. The course will provide both theoretical and practical background for the use of tissue cultures in complex pharmaceutical testing and biotechnological modification of cells and tissues. 7. Textbooks: Fundamental techniques in cell culture – a laboratory handbook (ECACC&Sigma), Tissue Culture Techniques: An Introduction by Bernice M. Martin (Paperback - Aug 1, 1994); Clinical Bone Marrow and Blood Stem Cell Transplantation by Kerry Atkinson Ed: 3, Cambridge University Press, 2004, Animal Cell Technology: From Biopharmaceuticals to Gene Therapy by Leda dos Reis Castilho, Routledge, 2008 Encyclopedia of Stem Cell Research by Clive Svendsen, Svendsen, Allison D. Ebert, Sage eReference, SAGE Publications, 2008


B.1. Specialty in Molecular Biotechnology Mandatory courses (10-25 credit) 1. Title of course: MOLECULAR AND FUNCTIONAL GENETICS, GENOMICS 2. Course coordinator: Dr. BÉLA MELEGH, professor 3. Credits: 4 4. Weekly classes: 1+3 5. Prerequisits: completed A1 foundation modul 6. Course description: Sequencing the whole human genome was a milestone in the history of modern medicine. Knowing the nucleotide sequences of all genes is not enough, though. Even more important is the discovery of funcions of genes and gene products, and the complex networks they are organised in. The gained knowledge would make it possible to taylor treatment to every person. 7. Textbooks: Genomics and Clinical Medicine (Oxford Monographs on Medical Genetics) by Dhavendra, M.D. Kumar and David Weatherall; Genomic and Personalized Medicine, Volume 1-2 by Huntington F. Willard, Geoffrey S. Ginsburg., Analysis of Genes and Genomes by Daniel L. Hartl, Elizabeth W. Jones, Ed: 7, Jones & Bartlett Publishers, 2008 1. Title of course: MOLECULAR BASIS OF PATHOGENESIS AND DISEASES, DIAGNOSTICS 2. Course coordinator: Dr. BÉLA MELEGH, professor 3. Credits: 3 4. Weekly classes: 1+2 5. Prerequisits: completed A1 foundation modul 6. Course description: Sequencing the whole human genom was a milestone in the history of modern medicine. During the project, a multitude of gene-polymorphisms were found, and subsequent targeted research increased their number even further. Such polymorphisms can both cause and protect us from a number of infectious and other diseases. Analysis of the genome of pathogens is also very important, as it can help diagnosis. A lot of pathogens can adapt very quickly to new environments, often with the help of mobile genetic elements (e.g. plasmide-coded antibiotic resistance of bacteria), or by high mutation rate (e.g. HIV). 7. Textbooks: 1. Genomic Imprinting and Uniparental Disomy in Medicine: Clinical and Molecular Aspects by Eric Engel, Stylianos E. Antonarakis; 2. Microbial Genomics And Drug Discovery by Steven J. Projan.; 3. Human Molecular Genetics by Tom Strachan, Garland Publishing, (2004); 1. Title of course: MOLECULAR TARGETING 2. Course coordinator: Dr .BALÁZS SÜMEGI, professor 3. Credits: 3 4. Weekly classes: 1+2 5. Prerequisits:completed A1 foundation modul 6. Course description: The course explains how recent understanding of molecular mechanisms in human pathologies -using genomics, proteomics and bioinformatics- have lead to identification of molecular targets in complex signal mechanisms, that can lead to potential drug target identifications. 7. Textbooks: Molecular Targeting and Signal Transduction (Cancer Treatment and Research) by Rakesh Kumar (2004), Target Discovery and Validation Reviews and Protocols: Emerging Molecular Targets and Treatment Options,Volume 2 (Methods in Molecular Biology) by Mouldy Sioud (2007), Tissue Proteomics: Pathways, Biomarkers, and Drug Discovery, by Brian C.-S. Liu, Joshua R. Ehrlich, Springer, (2008);


1. Title of course: MOLECULAR TOXICOLOGY 2. Course coordinator: Dr. PÁL PERJÉSI, associate professor 3. Credits: 4 4. Weekly classes: 1+3 5. Prerequisits: completed A1 foundation modul 6. Course description: The aim of the course is the presentation of the mechanisms of absorption, distribution and metabolism of exogenous molecules in the body, with special attention to rective metabolites and their interaction with cellular macromolecules. The course presents the molecular basis of the main toxic reactions, and the molecules causing non-organ-specific and organ-specific effects. The national and international regulations concerning toxic substances are also covered. 7. Textbooks: Casarett&Doull’s Toxicology. The Basic Science of Poisons. Sixth Edition. C.D. Klassen (Editor) McGraww-Hill, New York, 2001; Molecular Toxicology (Advanced Texts) by Nick Plant, Bios Scientific Publishers, 2003; Principles of Biochemical Toxicology by John Timbrell, Taylor&Francis, Ed: 3rd, Mechanistic Toxicology: The Molecular Basis of How Chemicals Disrupt Biological Targets by Urs A. Boelsterli, Taylor&Francis, 2003; Casarett’s and Doul’s: Toxicology, The basic science of poisons, C.D. Klaassen, Ed:7th, 2008 1. Title of course: PHARMACOLOGICAL TESTING USING IN VITRO HUMAN AND IN VIVO ANIMAL SYSTEMS 2. Course coordinator: Dr. LÓRÁND BARTHÓ, professor 3. Credits: 4 4. Weekly classes: 2+2 5. Prerequisits: completed A1 foundation modul 6. Course description: The students will be shown as many in vitro and in vivo tests as possible. Most of the tests will be carried out in practicals, some of the more complex test systems will be presented to them by the lecturer. In vitro: Effect of inflammatory mediators on smooth muscles. Prostaglandins, leukotrienes, platelet-activating factor, histamin, nitrogen-monoxide: their effects, antagonisation, blocking their biosynthesys. Analysis of mast cell mediated responses. The theoretical background of the experiments are illuminated in seminars. In vivo: Testing of analgesic and antinociceptive effects on mammals. Induction of hyperalgesia using general and specific irritants, testing anti-hyperalgesic effects. Induction and blocking of inflammation of immunological and other origin. The theoretical background of the experiments are illuminated in seminars. 7. Textbooks: Departmental lecture notes, Animal Models in Toxicology, Second Edition (Drug and Chemical Toxicology) by Shayne C. Gad (Hardcover - 2006), Advances In Animal Alternatives For Safety And Efficacy Testing by Harry Salem (Hardcover -1997) 1. Title of course: DRUG RESEARCH AND DEVELOPMENT 2. Course coordinator: Dr. ERIKA PINTÉR, associate professor 3. Credits: 2 4. Weekly classes: 2 5. Prerequisits: completed A1 foundation modul 6. Course description: The aims of the course are: showing the history of the drug research and modes of the drug development, presenting information about the phases of rational drug design, from the plan of the molecules to clinical trials.


7. Textbooks: 1. Textbook of Drug Design and Discovery, Third Edition by Povl Krogsgaard-Larsen, Tommy Liljefors, Ulf Madsen, New York, Oxford University Press, 2. Drug-like Properties: Concepts, Structure Design and Methods : from ADME to Toxicity Optimization by Edward Harvel Kerns, Li Di, Academic Press, 2008; 3. Drug and Biological Development: From Molecule to Product and Beyond by Ronald P. Evens, Springer, 2007; 4. Drugs: From Discovery to Approval by Rick Ng, Wiley-Blackwell, Ed:2nd 2008; 5. Guide to Drug Development: A Comprehensive Review and Assessment by Bert Spilker, Kluwer, 2008


B.2. Specialty in cell- and tissue-engineering) Mandatory courses (10-25 credit) 1. Title of course: MOLECULAR EMBRYOLOGY 2. Course coordinator: Dr. PÉTER BALOGH, associate professor 3. Credits: 4 4. Weekly classes: 2+2 5. Prerequisits: completed A1 foundation modul 6. Course description: The aim of this course is to over view development of main tissues and organs of mammals from a molecular developmental perspective. And also to highlight the importance of specific transcription factors and soluble agents in tissue differentiation and functional activity. 7. Textbooks: Developmental Biology, Eighth Edition, Scott F. Gilbert, 2006, Larsen's Human Embryology by Gary C. Schoenwolf, Steven B. Bleyl, William J. Larsen, Philip R. Brauer, Philippa H. Francis-West, Ed: 4, Churchill Livingstone/Elsevier, 2008, Introduction to Molecular Embryology by J. Brachet and H. Alexandre, Springer-Verlag Berlin and Heidelberg GmbH & Co. K 1. Title of course: BIOLOGICAL THERAPIES 2. Course coordinator: Dr. PÉTER NÉMETH, professor 3. Credits: 4 4. Weekly classes: 2+2 5. Prerequisits: completed A1 foundation modul 6. Course description: The aim of the course is to present the molecular level pathomechanism of diseases targeted by biological therapies, and to cover the targets of protein based medicinal products, and the methods used to produce these medicinal products. The course discusses in detail the medicinal products applied during substitution, immunosupressive, anti-viral, anti-tumor, anti-inflammatory therapies, and during therapy of autoimmune diseases. 7. Textbooks: 1. The Handbook of Biological Therapy by Bruce Kirkham, Arthur Kavanaugh, Scott Eric Plevy, and Jonathan Barker, Oxford University Press (2009) ISBN-10: 0199208166 2. Medical Biotechnology, editors: Pongracz J, Keen M, Elsevier (2009); 3. Molecular Markers of Brain Tumor Cells: Implications for Diagnosis, Prognosis and Anti-Neoplastic Biological Therapy by Bela Bodey, Stuart E. Siegel, and Hans E. Kaiser, Kluwer,2004 1. Title of course: TRANSDIFFERENTIATION 2. Course coordinator: Dr. PÉTER BALOGH, associate professor 3. Credits: 4 4. Weekly classes: 1+3 5. Prerequisits: completed A1 foundation modul 6. Course description: The aim of the course is to present the techniques of tissue transformation (e.g. establishment of immortalized cell lines) and practical uses of the techniques. The course will cover possibilities of genetic reprogramming in both differentiated cells and stem cells. The course will cover in detail the design and cloning of tissue specific transcription factors and promoters, and will present various methods for incorporating these elements into primary cells. The students for instance will learn about the procedures involved in production of hepatic tissue suitable for implantation from healthy pancreatic cells.


7. Textbooks: Stem Cell Biology: Development and Plasticity Ourednik, Jitka (Author) Sakaguchi, Donald (Author) Nilsen-Hamilton, Marit (Editor) Wiley & Sons, Limited, John, 2005, ISBN 1573315346, Pancreatic Cancer by Andrew M. Lowy, Steven D. Leach, Philip Philip, Springer, 2008, Clinical Bone Marrow and Blood Stem Cell Transplantation, by Kerry Atkinson, Ed: 3, Cambridge University Press, 2004 1. Title of course: THREE DIMENSIONAL TISSUE CULTURE TECHNIQUES 2. Course coordinator: Dr. JUDIT PONGRÁCZ, associate professor 3. Credits: 4 4. Weekly classes: 2+2 5. Prerequisits: completed A1 foundation modul 6. Course description: The course material is designed to provide theoretical background to currently employed three dimensional tissue culture techniques including scaffold-based (biodegradable) and scaffold-free (tissue printing, pelleting and hanging-drop) systems. The course will detail the importance of the tissue microenvironment in normal and arteficial tissue development. Directed cellular growth, organ formation for surgical implantation and drug target identification in human tissue systems and in vitro testing of pharmaceutical compounds in complex tissue systems are also part of the curriculum. The course will discuss the latest advances in technology and medicine for replacing tissues and organs damaged by disease and of developing therapies for previously untreatable conditions, such as diabetes, heart disease, liver disease, and renal failure.

7. Textbooks: Pongracz&Keen 2009. Medical Biotechnology, Elsevier; Tissue Engineering and Artificial Organs by Joseph D. Bronzino (Author); Cardiovascular Regeneration Therapies Using Tissue Engineering Approaches by Hidezo Mori (Editor), Hikaru Matsuda (Editor); Micro- and Nanoengineering of the Cell Microenvironment, Technologies and Applications (Engineering in Medicine & Biology) by Ali Khademhosseini (Editor); Tissue, Cell and Organ Engineering (Nanotechnologies for the Life Sciences) by Challa S. S. R. Kumar (Editor); Tissue Engineering: Engineering Principles for the Design of Replacement Organs and Tissues by W. Mark Saltzman; Principles of Tissue Engineering, 3rd Edition by Robert Lanza, Robert Langer, Joseph Vacanti ; Tissue Engineering: From Cell Biology to Artificial Organs by Will W. Minuth, Raimund Strehl, Karl Schumacher ; Medicine by Design: The Practice and Promise of Biomedical Engineering by Fen Montaigne;Forgacs&Newman 2005. Biologycal Physics of the Developing Embryo, Cambridge University Press, Cambridge; Principles of Regenerative Medicine by Anthony Atala, Ed: 3, Academic Press, 2008

1. Title of course: AUTOLOGOUS TISSUE TRANSPLANTATION 2. Course coordinator: Dr. TAMÁS ILLÉS, professor 3. Credits: 4 4. Weekly classes: 2+2 5. Prerequisits: completed A1 foundation modul 6. Course description: The goal of the course is to provide useful information regarding the clinical practice with tissue engineered products (e.g., cartilage, bone, skin, muscle, etc.). Furthermore, the class will discuss the strict regulation of all human research materials, how all human engineered products are to be handled, documented, and followed-up. The introduction of any biotechnological innovation into human clinical practice will be described in details. 7. Textbooks: Bone and Cartilage Engineering by Ulrich Meyer, Hans Peter Wiesmann-Publisher: Springer-Verlag New York, LLC Pub. Date: April 2006 ISBN-13: 9783540253471 264pp Edition Number: 1


Chondral Disease of the Knee: A Case-Based Approach by Brian J. Cole (Editor), M. Mike Malek (Editor) Publisher: Springer; 1 edition (August 10, 2006) ISBN-10: 038730830X ISBN-13: 978-0387308302


Elective courses (15-25 credits) 1. Title of course: BIOTECHNOLOGICAL METHODS IN FORENSIC MEDICINE 2. Course coordinator: Dr. KATALIN SIPOS, associate professor 3. Credits: 3 4. Weekly classes: 1+2 5. Prerequisits: completed A1 foundation modul 6. Course description: These days the importance of laboratory and molecular biology methods is obvious in the field of forensic medicine. These methods are used in the examination of different body fluids (blood, saliva, vaginal secretum). During the course we will discuss in details the methods used to analyze DNA (identifications, paternity testing): the significance of analyses of STRs, SNPs, and mitochodrial DNA. Besides the basic DNA methods we talk about new scientific approaches which are based on RNA and protein analyses (determination of course and time of death). 7. Textbooks: Arthur M. Lesk: Introduction to Genomics (Oxford University Press), The Forensic Casebook: The Science of Crime Scene Investigation by N.E. Genge, 2004, A Litigator's Guide to DNA: From the Laboratory to the Courtroom by Ron C. Michaelis, Robert G. Flanders, Jr., Robert G. Flanders, Paula Wulff, Academic Press, 2008; Genetic Testimony: Guide to Forensic DNA Profiling (Prentice Hall's Exploring Biology) by C. A. Spencer, 2003; 1. Title of course: APPLICATION OF BIOMARKERS IN PUBLIC HEALTH 2. Course coordinator: Dr. ZOLTÁN GYÖNGYI, assistant professor 3. Credits: 3 4. Weekly classes: 1+2 5. Prerequisits: completed A1 foundation modul 6. Course description: The aim of the course is to provide knowledge of identification and application of biomarkers, which can be the tools of primary, secondary and tertiary prevention. Initially, students will learn the basics of molecular epidemiology. Identification of high risk groups is possible by analysis of allele polymorphism, biomarkers of carcinogenic exposure and disease-specific molecules. Biomarkers, which are predominantly nucleic acids or proteins, can indicate early stages or progression of diseases. Students will come acquainted with tests which can be applied in the practice. 7. Textbooks: 1.Biomarkers in Risk Assessment: Validity and Validation: 223 (Environmental Health Criteria) by ILO, Unep, and Who, 2001, 2. Biomarkers of Environmental Contamination by John F. McCarthy and Lee R. Shugart, 1990, 3. An Introduction to Genetic Epidemiology (Health & Society) by George Davey Smith, Paul Burton, and Lyle J. Palmer, 2009 1. Title of course: APPLICATION OF HIGH THROUGHPUT SCREENING METHODS AND BIOROBOTICS IN BIOTECHNOLOGY 2. Course coordinator: Dr. PÉTER NÉMETH, professor 3. Credits: 3 4. Weekly classes: 1+2 5. Prerequisits: completed A1 foundation modul 6. Course description: The course is an introduction to the high content screening (HCS) technologies, including cellomics and multiplexed HCS assays. The technologies are widely used to determine multiple parameters which are not only measured within single cells using multiple reagents and morphometrics or analytical cytology, but relationships between the


parameter values are calculated, analysed and interpreted on a cell by cell basis. 7. Textbooks: (1) Taylor, D.L. et al. (Eds): High Content Screening, Humana Press, Totowa, New Jersey, 2007. (2) Shapiro, H.: Practical Flow Cytometry, Willey-Liss, Hoboke, New Jersey, 2003., (3) High Content Screening: Science, Techniques, and Applications by Steven A. Haney, Wiley, 2008 1. Title of course: ANTIBODY AND VACCINE PRODUCTION IN PLANTS 2. Course coordinator: Dr. GÁBOR JAKAB, associate professor 3. Credits: 4 4. Weekly classes: 2+2 5. Prerequisits: completed A1 foundation modul 6. Course description: The curriculum will cover the application of plants in biotechnology, including molecular biology, genetics and microbiology of plants and synthesis with mammalian systems. Transformation and gene delivery methods in plants: viruses, transposons, etc. Agrobaktérium transzformáció. Plant tissue culture, regeneration. Gene silencing. Genetically modified (GM) plants. Modification in plant metabolism and development to create plant „bioreactors” (molecular farming) and their advantages. Application of plant biotechnology in medicine. Formulation of rekombinant proteins, and their heterologous expression. Plantibodies, edable vaccines. Risk assessment of using transgenic plants, including case studies. 7. Textbooks: Plant Derived Vaccines by David Castle, 2008, Plant-produced Microbial Vaccines (Current Topics in Microbiology and Immunology) by Alexander V. Karasev, 2009, The 2007-2012 World Outlook for Manufacturing Vaccines, Toxoids, Blood Fractions, and Culture Media of Plant and Animal Origin Excluding Diagnostic Products by Philip M. Parker, 2006 1. Title of course: BASIC PRINCIPLES OF ELECTRON MICROSCOPY IN CLINICAL PRACTICE AND BIOLOGICAL RESEARCH 2. Course coordinator: Dr. LÁSZLÓ SERESS, professor 3. Credits: 2 4. Weekly classes: 1+1 5. Prerequisits: completed A1 foundation modul 6. Course description: Methods of electron microscopic fixation, embedding and sectioning and the use of the electron microscope. Demonstration of the subcellular elements in details and of a few tissues and organs. Demonstration of the electron microscopic methods used in the biological research. The use of electron microscopic methods in the clinical practice. The basic principles of electron microscopy will be discussed and we provide informations about the mode and the use of electron microscopy in the basic research and in the clinical practice. 7. Textbooks: 1. Dr Szabolcs Virágh: Ultrastructural pathology and diagnostic electron microscopy, 2.Leon Weiss:Histology. Cell and Tissue Biology, 3.Electron Microscopy: Principles and Techniques for Biologists by J.J. Bozzola, 1998 1. Title of course: INTRODUCTION TO GERONTOLOGY 2. Course coordinator: Dr. MIKLÓS SZÉKELY, professor 3. Credits: 2 4. Weekly classes: 2 5. Prerequisits: completed A1 foundation modul 6. Course description: The changing demographic profiles of modern societies have lead to a growing interest in understanding ageing. The course will discuss the biology of ageing and


longevity and special physiological, psychosocial aspects, age-related problems and methods of care may help adequate health provision for the elderly patients. 7. Textbooks: 1. The Merck Manual of Geriatrics (MSD, Rathway, 1990), 2. Principles of Geriatric Medicine and Gerontology by William R. Hazzard, John P. Blass, Jeffrey B. Halter, and Joseph G. Ouslander, 2003, 3. Hazzard's Geriatric Medicine & Gerontology: Sixth Edition (Principles of Geriatric Medicine & Gerontology) by Jeffrey B. Halter, William R. Hazzard, Joseph G. Ouslander, and Mary Tinetti, 2009 1. Title of course: ENZYME BIOTECHNOLOGY 2. Course coordinator: Dr. TÍMEA BERKI, associate professor 3. Credits: 2 4. Weekly classes: 2 5. Prerequisits: completed A1 foundation modul 6. Course description: The aim of the subject is to present the theoretical and practical background of enyzmebiotechnology. The course discusses in detail the biological functions and practical use of enzymes in life sciences. Participants will become familiar with the possibilities of isolation, production and manipulation of enzymes together with their applications. 7. Textbooks: 1. The Complete Book of Enzyme Therapy: A Complete and Up-to-Date Reference to Effective Remedies (Penguin Putnam) by Anthony J. Cichoke; 2. Enzyme-Prodrug Strategies for Cancer Therapy (Kindle Edition) by Roger G. Melton and Richard J. Knox 3. Biocatalysts and Enzyme Technology by K. Buchholz, V. Kasche, and U.T. Bornscheuer, Wiley, 2005 1. Title of course: LABORATORY DIAGNOSTICS 2. Course coordinator: Dr. ATTILA MISETA, professor 3. Credits: 2 4. Weekly classes: 2 5. Prerequisits: completed A1 foundation modul 6. Course description: Introduction into the fundamentals of clinical biochemistry, modern diagnostic procedures, measurement and data analysis systems. Student will become familiar with electrolyte measurements, enzyme and metabolite measurements, hematology measurement techniques, lipid metabolism, hormone diagnostics, tumor markers, molecular diagnostics. 7. Textbooks: 1. William J Marshall – Stephen K. Bangert, Clinical Chemistry, 2. Mosby's Manual of Diagnostic and Laboratory Tests (Mosby's Manual of Diagnostic & Laboratory Tests) by Kathleen Deska Pagana and Timothy J. Pagana, Mosby/Esevier, Ed:3rd, 2006 3. Understanding and Evaluating Commonly Requested Laboratory Tests by Gail Vaughn, Prentice Hall, 1999. 1. Title of course: MOLECULAR GERONTOLOGY 2. Course coordinator: Dr. JUDIT PONGRÁCZ, associate professor 3. Credits: 2 4. Weekly classes: 1+1 5. Prerequisits: completed A1 foundation modul 6. Course description: The curriculum covers physiological aspects of ageing and the molecular background of the ageing process, at cell, intracellular signaling and nucleic acid level. It highlights the potential molecular intervention possibilities to slow down the ageing process and decrease development of age related diseases. 7. Textbooks: 1. Molecular Mechanisms and Models of Aging Vol 1119, 2007. ANNALS of


the New York Academi of Sciences., 2. Molecular Gerontology: Research Status and Strategies by Suresh I.S. Rattan and Olivier Toussaint, 1997, 3. Molecular and Cellular Gerontology (Annals of the New York Academy of Sciences) by Olivier Toussaint, 2006, 4. Telomerase, Aging and Disease (Advances in Cell Aging and Gerontology) by M.P. Mattson, 2001 1. Title of course: PROTEIN EXPRESSION SYSTEMS IN LABORATORY AND INDUSTRY 2. Course coordinator: Dr .PÉTER NÉMETH, professor 3. Credits: 3 4. Weekly classes: 1+2 5. Prerequisits: completed A1 foundation modul 6. Course description: One of the objectives of the course is to present theoretical and practical knowledge of protein expression systems used for research purposes in one hand, including protein expression performed in bacterial, yeast, baculoviral vector driven insect cell, and mammalian cell based systems. The course will cover the vectors and molecular tags applied during the construction of fusion proteins, and will detail the techniques used for purification of recombinant proteins. The other major aim of the course is to cover recombinant protein expression on the industrial scale with special emphasis on the production of hormones, cytokines and antibodies applied in biological therapies. 7. Textbooks: 1. Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems, Edited by Gerd Gellissen, 2005., Wiley, ISBN: 3527310363 2. Medical Biotechnology, editors: Pongracz J, Keen M, Elsevier (2009) 3. Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems by Gerd Gellissen, Wiley, 2004 1. Title of course: NEUROBIOLOGY 2. Course coordinator: Dr. RÓBERT GÁBRIEL, professor 3. Credits: 4 4. Weekly classes: 2+2 5. Prerequisits: completed A1 foundation modul 6. Course description: The course will deal with the history of neurology and will introduce the main national and international research centres, publications and journals. The course material will build the understanding of neurobiology by characterizing the neuron – neuromorphology, molecular structure - and neural networks, including the function and structure of various glia cell types. 7. Textbooks: 1. Kandel-Schwartz: Principles of neural science, 2. Neurobiology: Molecules, Cells and Systems by G.G. Matthews, 2000; 3. Elements of Molecular Neurobiology, 3rd Edition by C.U.M. Smith, Wiley, Ed: 3rd 2002 1. Title of course: MODERN BIOPHYSICAL RESEARCH METHODS 2. Course coordinator: Dr. GÁBOR HILD, associate professor 3. Credits: 2 4. Weekly classes: 1+1 5. Prerequisits: completed A1 foundation modul 6. Course description: The course addresses the physics and biophysics-based research methods employed in life sciences. The practicals will familiarize the students with the methods, including a hands-on experience with the instruments. 7. Textbooks: 1.Introduction to Biophysical Methods for Protein and Nucleic Acid Research by Jay A. Glasel and Murray P. Deutscher, 1995, 2. Biophysical Labeling Methods in Molecular Biology by Gertz I. Likhtenshtein, 2008, 3. Methods in Modern Biophysics by B. Nölting, Springer, Ed: 2nd, 2005


1. Title of course: BASICS AND APPLICATIONS OF FLUORESCENCE MICROSCOPY 2. Course coordinator: Dr. GÁBOR HILD, associate professor 3. Credits: 2 4. Weekly classes: 1+1 5. Prerequisits: completed A1 foundation modul 6. Course description: The course will cover the basics of the fluorescence spectroscopy and some examples about their practical use. Lectures and practicals will cover the basics of fluorescence spectroscopy, fluorophores, fluorescence lifetime the effect of the environmental properties on the fluorescence, quenching, anisotropy, fluorescence resonance energy transfer, insights into the modern fluorescence microscopy, single molecule detection, fluorescence correlation spectroscopy. 7. Textbooks: Fluorescence spectroscopy in Biology, by Hof, Hutterer, Fidler, Springer, 2004 Principles of fluorescence spectroscopy (2006) – Joseph R. Lakowicz Molecular Fluorescence: Principles and Applications (2001) – Bernard Valeur 1. Title of course: NANOBIOTECHNOLOGY 2. Course coordinator: Dr LÁSZLÓ GRAMA, assistant professor 3. Credits: 3 4. Weekly classes: 1+2 5. Prerequisits: completed A1 foundation modul 6. Course description: The course described the foundations of a novel and rapidly developing field called nanobiotechnology. In nanobiotechnology, biotechnological (e.g., recombinant techniques) methods are combined with nanotechnology and single-molecule manipulation methods (e.g., single-molecule manipulation methods, optical tweezers). During the course the high-profile nanobiotechnology techniques will be demonstrated. 7. Textbooks: http://biofizika.aok.pte.hu/hu/oktatas/valaszthato/nanobiotech/ Nanobiotechnology: Concepts, Applications and Perspectives by C.M. Niemeyer and C. A. Mirkin, Wiley, 2004 Nanobiotechnology Protocols (Methods in Molecular Biology) by Sandra J Rosenthal and David W. Wright, Humana Press, 2005 1. Title of course: APPLIED VIROLOGY 2. Course coordinator: Dr. FERENC JAKAB, assistant professor 3. Credits: 2 4. Weekly classes: 1+1 5. Prerequisits: completed A1 foundation modul 6. Course description: The most important topics of this course: On the general features of the viruses and the detailed presentation of the viruses with a wild type. The opportunities of the molecular biology applications of the viruses, and the theoretical bases of the recombinant virus making. The classic's and modern virology diagnostic methods and their practical application. 7. Textbooks: 1.Bernard N. Fields (ED): Fields-Virology (two volumes) 4th Edition, Lippincott Williams & Wilkins (2001); 2. Virology: Principles and Applications by John Carter and Venetia Saunders, Wiley, 2007; 3. Principles of Virology: Molecular Biology, Pathogenesis, and Control of Animal Viruses by Jane S. Flint, Lynn W Enquist, and Anna Marie Shalka, Ed:2nd, 2003, 4. Human Virology by Leslie Collier and John Oxford, 2006


1. Title of course: IMMUNOPATHOLOGY 1. 2. Course coordinator: Dr. PÉTER NÉMETH, professor 3. Credits: 2 4. Weekly classes: 2 5. Prerequisits: completed A1 foundation modul 6. Course description: The course will start with anintroduction to the pathological background of immune related diseases. It will then discuss the basic mechanisms of autoimmune diseases, acquired or heredited immunodeficiencies, tumor immunology and the immunological problems of organ transplantations. The course will also provide an overview of the clinico-pathological analysis of different immunoregulatory failures and the present biotherapies and the future perspectives from the therapeutical monoclonal antibodies to the tumor vaccines. 7. Textbooks: 1.Immunology, Immunopathology and Immunity by S. Sell, 2001, 2. Immunopathology: Clinical laboratory concepts and methods (Series in laboratory medicine) by Robert M Nakamura, 1974 3. Tumor Immunology: Immunotherapy and Cancer Vaccines (Cancer: Clinical Science in Practice) by Karol Sikora, A. G. Dalgleish, and M. J. Browning, Cambridge University Press, 1996, Tumor Antigens Recognized by T Cells and Antibodies (Tumor Immunology) by Hans J Stauss, Yutaka Kawakami, and Giorgio Parmiani, CCR Press, 2003 1. Title of course: IMMUNOPATHOLOGY 2. 2. Course coordinator: Dr. SZEKERES-BARTHÓ JÚLIA, professor 3. Credits: 2 4. Weekly classes: 2 5. Prerequisits: completed A1 foundation modul 6. Course description: After reviewing the normal functions of the immune system the course focuses on mechanisms resulting in pathological functioning. The following topics will be covered; immunity to infections, anti-tumor immunity, reproductive immunity, transplantation immunology, autoimmunity, hypersensitivity, immunology of AIDS and neuroimmunology. 7. Textbooks:1. Clinical immunology, H. Chapel, M. Haeney, N. Misbah, Snowden, Blackwell, 2008, 2. HIV and AIDS: Basic Elements and Priorities by S. Kartikeyan, R.N. Bharmal, R.P. Tiwari, and P.S. Bisen, 2007, 3. Allergy in Practice by T. Platts-Mills and Johannes Ring, 2003, 4. Reproductive Immunology by Satish Kumar Gupta, Springer, 2000 1. Title of course: BIOPHYSICAL BACKGROUND AND CLINICAL APPLICATION OF ISOTOPDIAGNOSTIC AND RADIOTHERAPEUTIC METHODS 2. Course coordinator: Dr. KATALIN ZÁMBÓ, professor 3. Credits: 2 4. Weekly classes: 1+1 5. Prerequisits: completed A1 foundation modul 6. Course description: A detailed presentation will be given of modern physical biophysical and imaging methods to follow the pathway of radiopharmacons in different organs to earn precise morphological and functional results by the new multimodal systems (SPECT/CT, PET/CT). 7. Textbooks: 1.Radioactive Isotopes in Clinical Medicine and Research (Advances in Pharmacological Sciences) by H. Sinzinger, H. Bergmann, and Helmet F. Sinzinger, 1995, 2. Molecular Anatomic Imaging: PET-CT and SPECT-CT Integrated Modality Imaging by Gustav K. Von Schulthess, 2006, 3. Advances in the Radiotherapeutic Management of Carcinoma of the Prostate by A.V. D'Amico and G.E.H. Hanks, 1997


1. Title of course: INBORN ERRORS OF METABOLISM 2. Course coordinator: Dr TAMÁS DECSI, professor 3. Credits: 2 4. Weekly classes: 1+1 5. Prerequisits: completed A1 foundation modul 6. Course description: Inborn errors of metabolism represent rare, but altogether important diseases of paediatrics. Significant developments in therapeutic modalities during the last decade led to major improvement in the prognosis of the inherited diseases of metabolism. This course summarises basic knowledge about the diagnostics and therapy of inborn errors of metabolism. Practical examples of patients treated for these dieases in the Department of Paediatrics serve for discussing not only the way to diagnosis, but the longé-term clinical care as well. 7. Textbooks: 1. Inborn Errors of Metabolism in Humans by Forrester Cockburn and R. Gitzelmann, 1982; 2. Molecular Endocrinology (Human Molecular Genetics) by Franklyn F. Bolander, 2004; 3. Molecular Genetics of Endocrine Disorders by R. V. Thakker, 1998 1. Title of course: THEORY AND PRACTICE OF EVIDENCE BASED MEDICINE 2. Course coordinator: Dr. TAMÁS DECSI, professor 3. Credits: 1 4. Weekly classes: 1 5. Prerequisits: completed A1 foundation modul 6. Course description: Evidence-based medicine (EBM) offers practical tools to solve the practical problems arising in clinical settings as well as in outpatient care. This course is aimed not only to summarise the basic knowledge of EBM, but to provide practical examples of the usefulness of EBM as well. Formulating of clinical questions, literatue search strategies as well as critical appraisal of papers revealed by the search is dicussed in detail. 7. Textbooks: 1.EBM for PDAs: Evidence Based Medicine by Allan Platt, 2008, 2. Evidence-Based Medicine: How to Practice and Teach EBM (Book with CD-ROM) by Sharon E. Straus, W. Scott Richardson, William Rosenberg, and R.Brian Haynes, 2000; 3. The Evidence Based Practice Manual for Nurses (Elsevier science) by Jean V. Craig and Rosalynd L. Smyth; 2002 1. Title of course: CLINICOPATHOLOGY 2. Course coordinator: Dr. LÁSZLÓ PAJOR, professor 3. Credits: 1 4. Weekly classes: 1 5. Prerequisits: completed A1 foundation modul 6. Course description: During the course of pathology the clinicopathological view is emphasized in order to aid the synthesis of clinical and laboratory data and alterations detected by macro- and microscopical examination. The development of basic clinicopathological thinking and differential diagnostic skills is required by the end of the pathology curriculum, which is further supported by the demonstration of a case related to the subject of lectures every week. 7. Textbooks: S. L. Robins, V. Kumar: Basic Pathology, 7th edition, Saunders Company, 2003; Clinical Pathology (Oxford Core Texts) by James Carton, Richard Daly, and Pramila Ramani, 2006; Clinical Pharmacy and Therapeutics 4th edition, by Roger Walker, Catherine Whittlesea, Churchill Livingstone, 2007 1. Title of course: GENETIC TOXICOLOGY


2. Course coordinator: Dr .CSABA VARGA, associate professor 3. Credits: 1 4. Weekly classes: 1 5. Prerequisits: completed A1 foundation modul 6. Course description: Short description of the course: The objective of this interdisciplinary field is to demonstrate the environmental effects of the different chemical agents and their consequences and the strategy of the research. The main educational task of the subject: The course provides an up-to-date preventive approach including the role of environmental genotoxic effects as a cause of increasing frequency of cancers and congenital malformations. 7. Textbooks: Hulka BS, Wilcosky TC, Griffith JC.: Biological markers in epidemiology, Oxford University Press, 1990 Fairbairn DW, Olive PL, O’Neil KL (1995) The comet assay: a comprehensive review. Mutation Res., 339: 37-59.Venitt S, Parry JM eds. Mutagenicity testing,. IRL Press Ltd, Oxford, 1984 Kirkland, D.: Report on the International Workshop on Standardisation of Genotoxicity Test Procedures Maron, DM, Ames BN: Revised methods for the Salmonella mutagenicity test Mutation Res., 113, 173-215 (1983) 1. Title of course: STATISTICAL ANALYSIS 2. Course coordinator: Dr LÁSZLÓ PÓTÓ, associate professor 3. Credits: 3 4. Weekly classes: 1+2 5. Prerequisits: completed A1 foundation modul 6. Course description: The aim of the modern approach to statistics to develop a statistical way of thinking to analyse data correctly that leads to setting up new hypothesis, theories, analogies etc. The course will focuse on computer based calculations in statistical programs and understanding on real-life problems instead of memorizing and using statistical formulas of different methods. 7. Textbooks: Moore, D.S., The Basic Practice of Statistics, 3rd Ed. 2004. W.H. Freeman, Pótó, L. Biometrics (workbook), PTE ÁOK, 2007. Moore, David S., McCabe, George P. Introduction to the Practice of Statistics 5th Ed, 2005, W.H. Freeman Yates, Dan, Moore, David S., Starnes, Daren S. The Practice of Statistics (TI-83/89 Graphing Calculator Enhanced) 2/e, 2003, 1. Title of course: BIOMETRICS 2. Course coordinator: Dr LÁSZLÓ PÓTÓ, associate professor 3. Credits: 1 4. Weekly classes: 1 5. Prerequisits: completed A1 foundation modul 6. Course description: The course provides opportunities to practice problem solving in Biometrics using PC and the SPSS software. The aim is to handle typical medical problems at statistical level. This foundation is an important focus point of the Biometrics course. This course offers an organized way for extra practices. 7. Textbooks: Biometrics (workbook) - by L. Pótó PTE ÁOK 2007. Moore, D. S. The Basic Practice of Statistics, 3rd Ed., 2004. Moore, David S., McCabe, George P. Introduction to the Practice of Statistics 5th Ed, 2005, W.H. Freeman Yates, Dan, Moore, David S., Starnes, Daren S. The Practice of Statistics (TI-83/89 Graphing


Calculator Enhanced) 2/e, 2003, W.H. Freeman Rees, W. G. Essential Statistics, Chapman and Hall, 1992 1. Title of course: THEORETICAL AND CLINICAL ASPECTS OF NEUROENDOCRINE EXPERIMENTS 2. Course coordinator: Dr VALÉR CSERNUS, professor 3. Credits: 1 4. Weekly classes: 1 5. Prerequisits: completed A1 foundation modul 6. Course description: The course will discus some modern methods used in neuroendocrine research and their practical application. The course will include the following topics: in vitro methods in the neuroendocrine research, the theoretical backgrounds of the ligand binding assays (RIA, ELISA, TEGLIA) and their practical applications as well as the application and evaluation of the dynamic in vitro bioassays (perifusion, superfusion) and studying functions of endocrine glands (pituitary, pineal gland, gonads) Topics will also include the functional evaluation of hypothalamic releasing and inhibiting hormones and their analogs, the mechanism and biological significance of the circadian biological rhythms and finally in vitro bioassay studies on the avian pineal gland as a model of circadian biological clocks. 7. Textbooks: Circadian Physiology 2nd Ed (2005) by Roberto Refinetti, Taylor&Francis, Biological Rhythms (2002) by Vinod Kumar, Cecil Textbook of Medicine by Russell La Fayette Cecil, Lee Goldman, D. A. Ausiello, Ed 22, illustrated, revised, Saunders, 2004 1. Title of course: CAPILLARY ELECTROPHORESIS 2. Course coordinator: Dr. FERENC KILÁR, professor 3. Credits: 2 4. Weekly classes: 1+1 5. Prerequisits: completed A1 foundation modul 6. Course description: The course will include the following topics: capillary electrophoresis in modern separation science and application in health sciences, its theoretical background and applications, the design of capillary electrophoretic experiments for spoteins and small molecules and also the electrophoresis of cells. Finishing topics will be special applications, chiral separations and microbiology. 7. Textbooks: Handbook of Capillary Electrophoresis, ed. James Landers, 1994. CRC Press, Pharmaceutical Analysis: A Textbook for Pharmacy Students and Pharmaceutical Chemists, By David G. Watson Edition: 2, illustrated, Elsevier Churchill Livingstone, 2005 Metabolomics: The Frontier of Systems Biology, Masaru Tomita, Takaaki Nishioka, Masaru Tomita, Takaaki Nishioka, Edition: illustrated, revised, Springer, 2005 1. Title of course: ARCHEOGENETICS 2. Course coordinator: Dr. ISTVÁN EMBER, professor 3. Credits: 1 4. Weekly classes: 1 5. Prerequisits: completed A1 foundation modul 6. Course description: This course reviews the goals, history, results and methods of archaeogenetical research. Ancient DNA refers to DNA which can be recovered and analyzed from clinical, museal, archaeological and paleontological specimens. Ancient DNA ranges in age from less than 100 years to hundred thousand of years. The study of ancient DNA is a young field, but it has been revolutionized by the application of polymerase chain reaction technology, and interest is growing very rapidly. Fields as diverse as evolution, anthropology, medicine, agriculture, and even law enforcement have quickly found


applications in the recovery of ancient DNA. 7. Textbooks: Introduction to Population Genetics by Richard Halliburton, 2003, Principles of Population Genetics by Daniel L. Hartl and Andrew G. Clark, 1998, A Textbook of Genetics by H.S. Bhamrah, C.M. Chaturvedi, Anmol Publications PVT. LTD., 2002 1. Title of course: POPULATION GENETICS 2. Course coordinator: Dr. ISTVÁN EMBER, professor 3. Credits: 1 4. Weekly classes: 1 5. Prerequisits: completed A1 foundation modul 6. Course description: The course will include the following topics: introduction to population genetics (Hardy-Weinberg law, migration and population admixture, natural selection of various types and genetic drift), the Human Genome Project, Human Genome Diversity Project and Genographic Project, molecular phylogenetics (Y-chromosome and mitochondrial DNA), the analysis of nucleotide polymorphism and diversity as well as human variations and diseases. And the molecular epidemiology and genetic analysis of roma population. 7. Textbooks: Introduction to Population Genetics by Richard Halliburton, 2003, Principles of Population Genetics by Daniel L. Hartl and Andrew G. Clark, 1998, A Textbook of Genetics by H.S. Bhamrah, C.M. Chaturvedi, Anmol Publications PVT. LTD., 2002 1. Title of course: THE CYTOSKELETAL SYSTEM 2. Course coordinator: Dr .MIKLÓS NYITRAI, associate professor 3. Credits: 1 4. Weekly classes: 1 5. Prerequisits: completed A1 foundation modul 6. Course description: The course discusses the cytoskeletal system, a filamentous network that traverses the entire cell and participates in almost every cellular process. Biophysical, biological and biochemical properties of cytoskeletal filaments and associated proteins will be presented, as well as the experimental methods, with videomicroscopic recordings and practical examples. 7. Textbooks: Mechanics of Motor Proteins and the Cytoskeleton by Jonathon Howard, 2005, The Prokaryotic Cytoskeleton: A Written Symposium (Journal of Molecular Microbiology and Biotechnology) by S. Trachtenberg, 2006, Color Textbook of Histology by Leslie P. Gartner, James L. Hiatt Ed.: 3, illustrated, revised Saunders/Elsevier, 2007 Other elective courses (5-10 credits) 1. Title of course: COMMUNICATION IN SCIENCE 2. Course coordinator: Dr. PÉTER BALOGH, associate professor 3. Credits: 2 4. Weekly classes: 1+1 5. Prerequisits: completed A1 foundation modul 6. Course description: The aim of this course is the introduction of various forms of communication in biomedical science, and their critical evaluation. The curriculum will include oral presentation, paper and grant writing. While communication within the scientific world is important, presentation of scientific results, advances and theories are just as important towards the general public and the course material will also cover this area of science communication.


7. Textbooks: Writing And Presenting Scientific Papers - 2nd Edition. B. Malmfors, P. Garnsworthy & M. Grossman (2003), Science Communication in Theory and Practice - by Sue Stocklmayer, Kluwer Academic Publisher, A Field Guide for Science Writers - by Deborah Blum, Oxford University Press 1. Title of course: INTELLECTUAL PROPERTY LAW 2. Course coordinator: DR. ZSOLT GYÖRGY BALOGH, associate professor 3. Credits: 3 4. Weekly classes: 2+1 5. Prerequisits: completed A1 foundation modul 6. Course description: The course will cover the following topics: basic concepts of intellectual property rights; copyright, patents, trade marks; business models for exploitation of inventions;.rights of the author;.lLegal position of the patent holder; enforcement of patent claim; basic documents of intellectual property rights in Hungary and in the EU; the copyright act; the act on patent protection of inventions; the act on protection of trademarks; the TRIPS Agreement; the European copyright directive and the European Patent Convention. 7. Textbooks: W. R. Cornish: Intellectual property: Patents, Copyright, Trade Marks and Allied Rights. Fourth Edition. Sweet & Maxwell. London. 1999. Robin Jacob – Daniel Alexander – Lindsay Lane: A Guidebook to Intellectual Property Patents, Trade Marks, Copyright and Designs 5th edition. Sweet & Maxwell. London. 2004. Carlos M. Correa: Intellectual Property Rights, the WTO and Developing Countries. The TRIPS Agreement and Policy Options. Zed Books Ltd. London, New York. 2000. Creators’ Rights in the Information Society. ALAI Budapest 2003. Proceedings of the ALAI Congress September 14-17, 2003. KJK-Kerszöv Kiadványok. Hungarian ALAI Group & KJK Kerszöv, Budapest. 2004. Intellectual Property Law by Lionel Bently and Brad Sherman, 2008, Intellectual Property Law Q&A (Questions & Answers) (Questions and Answers) by Janice Denoncourt, 2007 1. Title of course: MARKETING IN BIOTECHNOLOGY 2. Course coordinator: Dr. GÁBOR PAULER, associate professor 3. Credits: 3 4. Weekly classes: 2+1 5. Prerequisits: completed A1 foundation modul 6. Course description: Biotech is one of the most capital intensive fields of research, where returns can be enermous in long term, however risk of getting results is also huge. Therefore, it is very important for a biotech researcher to identify target market of the research, its profit generating capacity, and expected financial returns on investment. In this course we introduce students into the qualitative and quantitative techniques of marketing research assessing the goal above. 7. Textbooks: PTE-PMMK Data Mining 1-2 Electronic Course Material, Introduction to Marketing, Theory and Practice by A. Palmer, 2004, Oxford University Press, The Oxford Textbook of Marketing, 2000, Ed. K. Blois, Oxford University Press, Strategic marketing creating competitive advances, D.West, J.Ford, E.Ibrahim, 2006, Oxford University Press 1. Title of course: GLOBAL BUSINESS STRATEGIES 2. Course coordinator: Dr PÉTER NÉMETH, professor 3. Credits: 1 4. Weekly classes: 1


5. Prerequisits: completed A1 foundation modul 6. Course description: The goal of the subject is to introduce large multinational biotechnological, pharmaceutical, and research and development (R&D) companies, and to get acquainted with their structure and function. Evaluation and analysis of global marketing strategies in different sectors. Students will learn the possibilities of founding, sustaining and developing of profit-oriented international and Hungarian R&D companies and the structural and functional characteristics of distributing companies in Hungary. 7. Textbooks: lecture notes, RegionalRTD&Innovation Policies and Practices, 2007., Thunderbird on Global Business Strategy by R.E. Grosse (Wiley), 2000, Hill, C., Global Business Today (Latest Edition), Hill, C.W.L. (2007). International Business: Competing in the Global Marketplace, (Sixth edition)

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A. A. CORE MATERIAL A.1. FOUNDATION MODULaok.pte.hu/docs/th/file/2012/tantervek_2012/course_eng_biotechnol... · inherited and aquired mutations and consequent genetic diseases. The