(A Joint Academic Program for Capacity Development in Flow

Prospectus-2015

TM BD-JH FACS Academy3rd Floor, Science Block,

Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi -110 062, India

www.bdbiosciences.com/in

(HAMDARD UNIVERSITY)

(Deemed to be University under Section 3 of the UGC Act, 1956

Accredited and reaccredited in ‘A’ Category in 2003 and 2011 respectively

Hamdard Nagar, New Delhi – 110 062

www.jamiahamdard.edu

JAMIA HAMDARD

(A Joint Academic Program for Capacity Development in Flow Cytometry in India)

TM FACS eLEARN

BD Biosciences, part of Becton, Dickinson and Company and Jamia Hamdard University have set up TM TM the BD-JH FACS Academy at Jamia Hamdard University in New Delhi, India. BD-JH FACS Academy is

an Industry/Academia collaboration that offers basic and advanced training courses, workshops and

seminars in flow cytometry to train young scientists and researchers on flow cytometry, a critical

component of research and clinical practice. BD has a long-standing commitment to help strengthen TMthe healthcare systems in India. The establishment of the BD FACS Academy is an addition to the

ongoing education and training programs designed to help increase the capacity of researchers and

clinicians on a sustainable basis. The 3,000 square-feet, state-of-the-art facility in New Delhi, is

equipped with a comprehensive range of modern flow cytometers, including the BD FACSAria™, BD™

LSR II, BD FACSCalibur™, BD FACSVerse™ and BD Accuri™ C6 personal flow cytometer, which provides

flow cytometry training to approximately 250 researchers and clinicians in a year. Aligned with its

commitment to flow cytometry, BD Biosciences, India has established Centers of Excellence in flow

cytometry through collaborative partnership with several academic institutes of repute with and aim

to develop and jointly organize workshops, seminars and training programs on flow cytometry.

Jamia Hamdard is a University with distinction of being a Health Science University. It got the

deemed to be University status in 1989. Over the years, Jamia Hamdard emerged as an out standing

institution of higher learning with distinct and focused academic programs in Pharmacy, Basic

Sciences, Medical Science and allied disciplines including Nursing, Physiotherapy and Occupational

Therapy, besides contributing to higher education in Information Technology and Computer

Applications as well as Business Management. JH is well known for its postgraduate and doctoral

programs. The various faculties and the departments of the University are equipped with advanced

educational and research facilities particularly in the area of health sciences. The faculties publish

hundreds of research papers every year and run many different projects funding by all major agencies.

The University has been accredited two times in ‘A’ category by NAAC.

TM BD-JH FACS Academy

3rd Floor, Science Block, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi-110062, India

TM Phone: +91-11-26059688,Extn; 5568,5560,5478 (JH), 29968338 (BD-JH FACS Academy)

Program Coordinators: Dr. S. Raisuddin (JH); Dr. Paresh Jain (BD)

Email: [email protected], [email protected]

Website: www.jamiahamdard.edu, www.bdbiosciences.com/in

TM FACS eLEARN Prospectus-2015

BD,BD logo and all other trademarks are the property of Becton, Dickinson and Company. ©2015 BD


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It gives me great pleasure to note that BD Biosciences, BD India Private Ltd and Jamia Hamdard University TMhave expanded their educational efforts for teaching of flow Cytometry by setting up the FACS eLEARN

Program. This should go a long way in expanding the opportunities for students from all over India and the

neighboring countries to get proper training in applications of this important technology through the

internet.

The Indo-US Cytometry Workshops have a long standing partnership with BD Biosciences in India and the

leadership of Jamia Hamdard especially Dr. G.N. Qazi who was one of the first to host an Indo-US Cytometry

Workshop as the director of the Regional Research Labs in Jammu. We are looking forward to our continued

collaboration by holding future Indo-US workshops at Jamia Hamdard University and hope the students who TM

participate in the FACS eLEARN program will attend these to expand on their training.

I wish this joint effort best of luck as it will go a long way to fill the need for continuing education in

applications of flow Cytometry in biomedical research and patient monitoring.

(Awtar Krishan)

Message

Dr. Awtar KrishanEmeritus ProfessorUniversity of Miami

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I am happy to note that our collaboration with Becton Dickinson (BD) Biosciences is going to take a new

dimension with introduction of joint educational programs in Flow Cytometry. Jamia Hamdard has strived to

develop strong linkage with industry. We highly value such a relationship with the partners who are

internationally acclaimed for their R&D and Corporate Social Responsibility (CSR) efforts. BD is a world leader

in flow cytometry. Besides, they are also known for their highly successful efforts in creating trained human TM resource in flow cytometry. The BD-JH FACS Academy at Jamia Hamdard which is one of its kinds in this

TM region of continent is a vibrant hub of activities. Training programs of the FACS Academy are highly popular.

TM Researchers from all over India and other countries have been trained at the FACS Academy. I am confident TM

that academic programs named as FACS eLEARN initiative will be unique blend of theoretical knowledge

and hands-on exposure to the wonderful technique of flow cytometry which has found its increasing new

applications in the fields of science and medicine. The curriculum for these programs is very comprehensive

which will enable researchers to fully understand functioning and applications of the technique. There is no TMplace like BD-JH FACS Academy in this country where such a large range of state of art flow machines are

under one roof. During hands-on assignment researchers will have opportunity to try their hands on many of

them.

I congratulate team at BD and Jamia Hamdard Coordinator of the program for conceiving this initiative and

wish all success to it.

(G.N. Qazi)

Message

Dr. G. N. QaziVice-ChancellorJamia Hamdard



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BD is a leading medical technology company that partners with customers and stakeholders to address many

of the world’s most pressing and evolving health needs. BD Biosciences is a business segment of BD which

focuses on bringing innovative diagnostic and research tools to scientists, clinical researchers, laboratory

professionals and clinicians who are involved in basic research, drug discovery and development,

biopharmaceutical production and disease management. What differentiates BD Biosciences from other

players in this category is its high-caliber technical and engineering support which enables the customers to

get the best out of the systems and solutions that is offered.

TM The establishment of the BD-JH FACS Academy at Jamia Hamdard in 2012 was an inspiring initiative to our

ongoing education and training programs designed to help increase the capacity of young scientists,

researchers and clinicians on a sustainable basis. Designed to bring flow cytometry learning at your pace and

place, we are now pleased to offer on-line certificate and diploma courses in collaboration with Jamia

Hamdard University and Dr Mike Ormerod. The courses will offer you a sound understanding of the principles

and technology of flow cytometry thus enhancing your insight into flow cytometry as a critical research and

diagnostic tool.

I wish this program huge success, and through this commendable initiative taken by BD and Jamia Hamdard

teams, I look forward to developing a network and talent-pool of highly motivated individuals who are well-

trained in science and art of flow cytometry.

(Varun Khanna)

Message

Varun KhannaManaging DirectorBD INDIA Pvt. Ltd.

Since 2001, I have taken part in the INDO-US Cytometry Workshops organised by Dr. Awtar Krishan. During

those years there has been an enormous growth in the number of flow cytometers in India. There has also

been a concomitant growth not only in the number of competent operators but also in the quality of work

being produced.

The demand for training has also grown and it is now an opportune time for a new certificate and diploma in TM

Flow Cytometry. I am delighted to work with BD India and Jamia Hamdard on the FACS eLEARN initiative to

continue to drive up the standard of work carried out in laboratories in India.

( Michael G. Ormerod)

Message

Dr. Michael G. Ormerod

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TM FACS eLEARN

TM FACS eLEARN is a joint program where industry and academia have come together to develop capacity in one

of the most advanced techniques in Biomedical Sciences, that is, Flow Cytometry. There were days in India

when Flow Cytometers were limited in number as it was an expensive instrument and reagents were not

readily available. However, in last decade Flow Cytometry has gained momentum. The applications of Flow

Cytometry have been expanded and are now found in basic science, applied science, medical science and

plant science. Now flow cytometers have been installed in several research institutions, hospitals and

universities.

At various scientific forums, a need has been felt that India should have trained professionals who are able to

handle Flow Cytometers and interpret effectively the generated results. Since BD is associated with Flow

Cytometry, it has always perceived this need and has been actively involved in training professionals in this

field of advanced technology.

TMBD and Jamia Hamdard established a Flow Cytometry training and education facility named “BD-JH FACS nd TM

Academy” on 2 March 2012. The FACS Academy has been established in Jamia Hamdard campus with

state-of-art Flow Cytometers under one roof. It has trained 350 users across the country and overseas. The

response has been overwhelming. However, the routine operator training comprises a 2-4 days module.

Through the feedback of trainees as well as from various users and resource persons it has emerged that a

structured academic program would be immensely helpful. Moreover, a Certificate or Diploma course will not

only be helpful for their career in this field but also help them recognized nationally and internationally as TM

Certified User of this technology. Therefore, the FACS Academy has launched academic programs under the TMFACS eLEARN initiative to fulfill such a need.

TM Web Based Training Program - FACS eLEARN programs are designed for professionals who are already in a

job or a regular academic program and may not be available for regular contact time with the faculty. Hence, a

web based training program with e-mail support provides a ready access and opportunity to learn basic and

advanced Flow Cytometry at one’s own pace and convenience. The web module and e-mail support program

will be followed by a hands-on experience on Flow Cytometry instruments and applications.

In this e-learning format we have developed an alliance with Dr. Michael Ormerod; a UK based Scientist

renowned in the field of Flow Cytometry. He has authored or co-authored about 200 scientific publications

and a book “Flow Cytometry - A Basic Introduction”. He conducted a five day course on Flow Cytometry for the

Royal Microscopical Society for twenty years and still teaches on the course. He has also organized and/or

taught on courses in various countries including India. Furthermore, he ran a Distance Learning Course on

Flow Cytometry for the University of Ulster, UK for many years. He also runs a short introductory e-course on

Flow Cytometry for the Royal Microscopical Society.

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Courses

Detailed Course Content

TM The following programs will be offered through the FACS eLEARN initiative jointly by BD Biosciences and Jamia Hamdard.

To do the course, you need to have access to PC-compatible computer running Windows. You can only complete the

course if you have an understanding of how to work in a Windows OS environment on a PC. If you are not familiar with

Windows, you need to do a 'crash' course to bring yourself up-to-date on the Windows OS environment before tackling

any of the unit. In addition to the text and illustrations, you will be able to access example data files of typical applications.

You will be asked to analyze these files using a downloadable computer program.

Certificate Course in Flow Cytometry (CCFC)-

The web based certificate course comprises 5 units with assignments and 5-days hands-on program including evaluation

and examination. For the certificate course, first four units (compulsory); and one more unit, either the Unit 5 (Some

clinical applications) or the Unit 6 (DNA and the cell cycle) needs to be completed.

Advanced Diploma in Flow Cytometry Techniques (ADFC)-

The web based diploma course comprises all 9 units with assignments and 5-days hands-on program including evaluation

and examination.

ADFC 1 - Candidates who have successfully completed CCFC will need to complete only additional 4 units of the advanced

course.

ADFC 2 - Candidates who directly enroll in Advanced Diploma course will have to complete all 9 units.

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Intakeper batch

Course fee (INR)

and must have at least one year hands-on experience on flow cytometry

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Unit Description:

Unit 1. Introduction

Unit 2. The flow cytometer

Unit 3. Data analysis

Unit 4. Immunofluorescence

Unit 5. Some clinical applications

Unit 6. DNA and the cell cycle

Unit 7. Other applications

Unit 8. Cell proliferation

Unit 9. Cell death

This unit covers what is flow cytometry? It describes the principles underlying the technique, explains its advantages and

disadvantages and outlines the major applications.

On completing the unit, you should know about the components of a flow cytometer and be aware of the main features of

the instrument.

When you have finished this unit, you should be familiar with the major features of data analysis programs. You should

also have a clear understanding of how flow cytometric data is analyzed, using 'regions' and 'gates'.

The Unit describes the methods used for labeling cellular antigens using specific antibodies, the properties of the

different fluorochromes used to label antibodies, the problems arising from the overlap of the fluorescence spectra and

the method used to correct for this spectral overlap. It finally examines some quantitative aspects of flow cytometry.

This Unit introduces you briefly to most of the major applications of flow cytometry in the clinical laboratory such

asImmunophenotypic analysis of leukemia and lymphoma, Detection of minimal residual disease, Stem cell

enumeration, Solid organ transplantation, Detection of autoantibodies, HIV infection, Foeto-maternal haemorrhage,

Immunodeficiency diseases, Paroxysmal nocturnal haemoglobinuria, Reticulocyte analysis, Platelet counting and

function, Measurement of phagocytosis.

At the end of the Unit, you should understand how flow cytometry can be used to measure DNA ploidy and to analyze the

cell cycle. You are introduced to a computer program used for cell cycle analysis.

The Unit describes other techniques used in flow cytometry, which might be used in a variety of applications such as

Fluorescent proteins, Loading dyes into cells, Kinetic analysis of intracellular enzymes, Measuring membrane

permeability, Following changes in membrane potential, Using fluorescence ratiometric methods, measuring

intracellular calcium ions, Measuring intracellular pH, Measuring intracellular glutathione, Measuring intracellular

oxidative species, Measurement of drug uptake, Long term labels for cells, Analyzing and sorting chromosomes,

Microbead technology identification of stem cells using 'side population' (SP) analysis.

In this Unit, you will learn about methods used to follow the movement of cells through the cell cycle, in vitro and, for

tumors, in vivo.

The last unit looks at cell death. It will show you how a variety of flow cytometric methods can be applied to its study. You

will also learn how these methods might be applied in clinical research.


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Course calendar

Fee Structure

Mode of Fee Payment

Assessment Procedure

rd The web based learning course will start from 3 August 2015 and 5-days contact program inclusive of evaluation and th th

examination will be held between 16 November 2015 and 18 December 2015.

ndCommencement of Online Application Forms* 2 February, 2015

thLast date for Receiving Completed Application Form 30 July, 2015

stIntimation to the selected candidates by e-mail 31 July, 2015

rdCommencement of Web based course 3 August, 2015

th th5-days contact program inclusive of evaluation and examination** 16 November to 18 December 2015

*Enrollment in the course will be strictly on first-come, first-serve basis

**Exact dates (5 days) will be intimated to individual participants before 2 months of the contact program. A batch of around 6 students will be made to work on Flow TM Cytometer during contact program at BD-JH FACS Academy.

Name of Program Course Fee in Indian Rupees

Certificate Course in Flow Cytometry (CCFC) 30,000

Advanced Diploma in Flow Cytometry Techniques (ADFC 1)* 30,000

Advanced Diploma in Flow Cytometry Techniques (ADFC 2) 50,000

*Participants who have successfully completed CCFC are eligible for ADFC 1

The course fee includes access to the online course, copy of the text book on Flow Cytometry authored by Dr. Michael G.

Ormerod, hands-on training charges, examination and evaluation charges and light refreshment during hands-on TM

assignment at BD-JH FACS Academy.

The course fee should be paid either through a crossed cheque/demand draft in favor of “Jamia Hamdard” payable at

New Delhi. In special circumstances request for payment of fee may be accepted in two installments. The first installment

will be of Rs. 20000 for CCFC or ADFC 1 and Rs. 35000 for ADFC 2. Balance will be required to be paid before

commencement of hands-on program of the respective course.

A candidate may withdraw from the course within 30 day of registration. In this case fee will be refunded after deduction

of Rs. 1000. No request for withdrawal will be entertained after 30 days of registration and no fee refund will be made.

The enrolled candidates will be assessed for their proficiency throughout the duration of the course by the online course

coordinator. The coordinator will assess the candidate for their proficiency on the basis of the submitted assignments. TM

After completion of hands-on program a term-end written and oral evaluation will be conducted at BD-JH FACS

Academy to test the theory and practical knowledgebase of the candidates.


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Results & Certification

Accommodation

Course Workflow

Successful candidates will be issued a certificate based on their performance in submitted online assignments and in the

term-end evaluation. The term-end examination shall be comprised of questions and problems to test the skills learned TM

by them during the hands-on exposure at the FACS Academy as well as from the online assignments. The candidate will

be graded on the scale of Semester Grade Point Assessment (SGPA) as per the university norms. Candidates who are

unable to obtain the required SGPA in the first attempt will be allowed to reappear for one more attempt to obtain the

desired score. For this purpose they will be required to pay an examination fee of Rs. 5000 along with an application for

reassessment. The re-examination will be conducted along with subsequent batches. Those who fail to obtain the

required grade in two consecutive attempts will be required to register afresh.

TMAccommodation may be arranged for outstation candidates attending the hands-on module at BD-JH FACS Academy in

the Scholar’s house of Jamia Hamdard on payment of prescribed charges by the course participant. University Scholars’

house accommodation depends on availability and first-come, first-serve basis. Preference will be given to female

candidates attending the course in allocating the on-campus accommodation.

Online registration

Provisional confirmation

Payment of fee by the confirmed

candidate

Final confirmation

Access to online content

Provisional allotment

of dates for hands-on module

Successfulcompletionof all web

assignment

Hands-on module at BD-JH

TMFACS Academy,

New Delhi

Term-end evaluation

Declaration of results

Award of certificate

Confirmation of dates for

hands-on module


Prospectus-2015

(A Joint Academic Program for Capacity Development in Flow Cytometry in India)

TM FACS eLEARN

Previous batch scholars’ view about the program

Would you tell us a little more about your experience through this program

Dr. Uday Sudhalker is DNB in Pathology and works as a Specialist Pathologist at Lifeline Hospital, Oman "

“FACS eLEARN program included the basic flow cytometry book by Dr. Ormerod, online usage of software to complete the assignments and self-assessments that made this course a complete package and easy to understand. It was really a good experience to be with such a knowledgeable faculty who covered as many and research applications as possible”.

Dr. Samir Agarwal is MD Pathology and currently working as a long term trainee at Dept. of Transplant Immunology at AIIMS, New Delhi.

“My experience of the course was very enjoyable and a great learning curve. The course content was well designed and executed. The faculty members came across as extremely knowledgeable, ever willing to lend an ear to queries and provide solutions. The hands-on module was very well organised and designed to provide an overview of the spectrum of applications of flow cytometry”.

Dr. Kaneeka Bhatnagar is MD Pathology and works as a Senior Pathologist at DMCH, Ludhiana.

“It was a wonderful experience at FACS academy. This course was like a foundation course making understand flow cytometry in a simplified way. During the course, we were provided exposure to various available instruments”.

Would you tell a colleague about the program

Dr. Uday Sudhalker: “I will definitely recommend my friends and colleagues to opt such and informative and useful certificate/ diploma course”.

Dr. Samir Agarwal: “I have discussed about the course and its relevance in advancing my understanding of the science of flow cytometry, with my colleagues”.

Dr. Kaneeka Bhatnagar: “I would strongly recommend this course as the basics and different aspects are covered simultaneously. Being a pathologist myself, this course has given me confidence to deal with a flow cytometer and its various aspects. The technical stuff is as much important as the data analysis and for a good analysis, understanding of the instrument is really important”.

Documents

(A Joint Academic Program for Capacity Development in Flow