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About OMICS GroupAbout OMICS Group
OMICS Group International is an amalgamation of Open Access
publications and worldwide international science conferences and events.
Established in the year 2007 with the sole aim of making the information
on Sciences and technology ‘Open Access’, OMICS Group publishes 400
online open access scholarly journals in all aspects of Science,
Engineering, Management and Technology journals. OMICS Group hasEngineering, Management and Technology journals. OMICS Group has
been instrumental in taking the knowledge on Science & technology to the
doorsteps of ordinary men and women. Research Scholars, Students,
Libraries, Educational Institutions, Research centers and the industry are
main stakeholders that benefitted greatly from this knowledge
dissemination. OMICS Group also organizes 300 International
conferences annually across the globe, where knowledge transfer takes
place through debates, round table discussions, poster presentations,
workshops, symposia and exhibitions.
About OMICS Group ConferencesAbout OMICS Group Conferences
OMICS Group International is a pioneer and leading science event
organizer, which publishes around 400 open access journals and
conducts over 300 Medical, Clinical, Engineering, Life Sciences,
Phrama scientific conferences all over the globe annually with the
support of more than 1000 scientific associations and 30,000 editorial
board members and 3.5 million followers to its credit.board members and 3.5 million followers to its credit.
OMICS Group has organized 500 conferences, workshops and national
symposiums across the major cities including San Francisco, Las Vegas,
San Antonio, Omaha, Orlando, Raleigh, Santa Clara, Chicago,
Philadelphia, Baltimore, United Kingdom, Valencia, Dubai, Beijing,
Hyderabad, Bengaluru and Mumbai.
An application of electron beam
lithography: location of metallic
nanostructures based on .
Enrique SamanoCNyN-UNAM, Ensenada, B.C., México
3rd International Conference on Materials Science & Engineering-2014
OUTLINE• is the carrier of genetic information. It is also “brick and mortar”
for building at the nanoscale (N. Seeman, Nature 421, 427- 431 (2003)).
• DNA belongs to the nanometer scale, its diameter is approximately 2 nmand one full turn of the helix is about 3.5 nm (~ 10.5 bp long). Complexmaterials with micron-scale dimensions and nanometer-scale featureresolution can be created via engineered DNA self-assembly.
• These assemblies are increasingly being exploited as templates for theprogrammed assembly of functional inorganic materials.
• A scheme has been recently proposed to apply these bioinspired DNAtemplates towards the fabrication of composite materials for use inphotonics, medicine, etc. (E. Samano et al., Soft Matter (2011)).
• A method for producing metallic nanostructures with a programmabledesign based on the specific positioning of AuNPs (5 nm size) on DNAorigami is shown. The AuNPs can be enlarged by coalescence of Ag in acontrolled manner by metallization (E. Samano et al., Nano Lett. (2011)).
DNA ORIGAMI
• Origami is the Japanese art ofpaper folding. Flat sheets canbe folded into 3d-shapes.
• DNA origami is a 2d-nanostr.based on the ss-M13mp18viral genome with 7 249 bases.viral genome with 7 249 bases.
• This plasmid is folded in aplanned and accurate mannerby ~200 specific complement.“staple” strands of DNA (P.
Rothemund, Nature 440, 297-302 (2006)).
+M13mp18
225 fold excess
helper strands in
TAE/Mg2+(1x, pH8)
a) 90 °C for 5 min
in thermal cycler
b) 4 °C ( -1 °C/min)
c) Stored at 4 °C
DNA ORIGAMI
Self-assembly of Rectangular DNA Origami
90
nm
70 nm
DNA
origami
Soln.
2 nm height
Purification
Microcon YM-100
Centrifugal Filter
Unit,100K MWCO.
Strategy on rectangularorigami to organize :
• Biological materials and other chemical groups
AuNP:DNA ORIGAMI CONJUGATES
• Quantum dots and Magnetic nanoparticles
• Carbon nanotubes
• Metal nanoparticles (NPs)
AuNP:DNA ORIGAMI CONJUGATES
• DNA origami has the potential to act as a template for the accurate array of AuNP to produce precisely engineered DNA nanostructures.
• This is possible because the “sticky ends” from the origami can be extended at binding sites enabling the attachment of complementary DNA-functionalized AuNPs; i.e, modified DNA origami is conjugated.
• Later, the size of the AuNP bound to DNA origami can be enlarged by reducing Ag ions using a kit (initiator, moderator and activator) at R.T.
ORIGAMI + AuNP ON EACH CORNER
AFM image of AuNP + DNA (1.4:1) on SiO2 in air. Each particle is attached to origami by two binding sites. Scale bar, 250 nm.
PATTERN GENERATION BY e-BEAM• The Nanometer Pattern Generation System (NPGS) has been designed to
delineate complex structures with sizes from nanometers up to microns.
• There are three basic steps to generate a pattern by e-beam lithography:
i. Pattern design, ii. Parameter run file creation, iii. Pattern writing.
SiO2
Si
Scheme to generate the alignment marks and electrical leads by NPGS
Si
PATTERN GENERATION BY e-BEAM
Design of a 100 µm x 100 µm pattern to write 100 nm x 80 nm rectangles, 5 µm apart, by e-beam lithography to place metallized DNA origami in each.
ORIGAMI + 2 AuNP ON 2 CORNERS
AFM image of AuNP + DNA (1.5:1) on SiO2 in air for plasmonicsexper. Each particle is attached to origami by two binding sites.
ORIGAMI + 2 AuNP ON 2 CORNERS
SEM image showing the 100 nm x 80 nm rectangles, 5 µm apart, carved on SiO2 in order to immobilize Au NPs after 10 min. of metallization (~ 40 nm size).
PLASMONICS• There is a great interest in the study of the optical properties of metallic
nanoparticles and their aggregates in the nanoscale vicinity.
• Plasmon is a collective oscillation of the valence electrons in a metallicparticle. The resonance frequency depends on the material composition(mainly electron density) and the surrounding dielectric medium.
• In particular, Ag and Au exhibit d → s interband transition in the UV• In particular, Ag and Au exhibit d → s interband transition in the UVregion that mixes with the plasmon resonance and shifts it to the visible.
• In nanoparticles (NPs), the plasmons strongly couple to optical fields,producing intense light-scattering spectra. The intensity of the spectrafrom noble-metal NPs is highly dependent to particle size and shape.
• The light-scattering spectrum from a single noble-metal NP of 20 nm orlarger can be readily observed by dark-filed microscopy. As two metalNPs are brought together, their plasmons couple and the resonance freq.changes as a function of particle separation. A red shift will occur due toconstructive interference between the enhanced fields of each NP.
DARK FIELD MICROSCOPY
Experimental conditions:i) Unpolarized white light from a tungsten lamp.ii) The sample is placed on a glass slide.iii) A 5 µm size aperture is used to collimate the transmitted light.
PLASMONICS APPLICATIONS
M. I. Stockman, Phys Today (Feb), 39-44 (2011)
Sensing. The spectral red shift of localized plasmonic resonances due to the aggregation of metal NPs can be applied in sensing, HPT.
PLASMONICS APPLICATIONS
Nanolens. “Hot spots” in a system composed of 3 Ag NPs in a row whose sizes form a decr. geometric series. “Hot spots” are spikes of intense and highly localized fields.
M. I. Stockman, Phys Today (Feb), 39-44 (2011)
Sensing. The spectral red shift of localized plasmonic resonances due to the aggregation of metal NPs can be applied in sensing, HPT.
SUMMARY
Controlled placement of NPs on DNA-Origami
Controlled Ag metallization of NPs placed on DNA-Origami
Plasmon coupling for two Ag
metallized NPs on DNA-Origami
Let Us Meet Again
We welcome you all to our future conferences We welcome you all to our future conferences of OMICS Group International of OMICS Group International
Please Visit:Please Visit:
httphttp://materialsscience.conferenceseries.com://materialsscience.conferenceseries.com//
Contact Contact us atus at
materialsscience.conference@[email protected]
[email protected]@omicsgroup.com