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EVERY STEP OF THE WAY

AccuGENX-ID® BacSeqAccurate identification of bacterial isolates is critical to environmental monitoring programs. Charles River’s AccuGENX-ID®

BacSeq, bacterial identification service, utilizes comparative sequencing of the 16S ribosomal RNA (rRNA) gene in bacteria,

which has been proven to be the most accurate method for identifying unknown organisms.

The Advantages of Genotypic AnalysisFor many organisms, traditional phenotypic identification is problematic, takes a great amount of time, and can result

in subjective interpretation of the test results. Not all strains within a given species consistently exhibit a particular

characteristic, thereby limiting phenotypic identification methods. Additionally, library databases utilized in support of

phenotypic identification are limited and challenging to update. Our genotypic analysis process stems from the reference

method used by taxonomists. It analyzes DNA sequence data through a conventional assembly process, which includes

auto-assembly and manual review, and correction of base caller errors.

The BacSeq sequencing technique is independent of growth conditions or viability of the isolate because it only needs

genomic DNA from your microbe. The resulting DNA sequence of each sample submitted for identification is compared to

the validated Accugenix® library – the industry’s most comprehensive and up-to-date database for environmental isolates –

and the resulting phylogenetic analysis is unparalleled in the industry.

The AccuGENX-ID® Database and Accugenix® Validated LibrariesCharles River is committed to providing the most accurate identifications to the industries we serve. This commitment

requires that we continuously update our Accugenix® validated libraries to stay current with an evolving microbial world.

Since our objective is to achieve the highest percentage of species-level identifications, we compare sequence data from

samples that do not result in a species-level match against a variety of publicly available sequence databases. If a newly

described organism is a good match for those sample sequences and is validly named and published, our scientists

proceed with our validated library entry process.

MICROBIAL SOLUTIONS

Advantages:

• Over 100,000 bacterial samples identified each year

• Industry’s highest reportable rate of 98%

• Rapid results, including same-day, with a 99.6% on-time delivery rate

• Validated bacterial library with the most relevant species entries for manufacturing facilities

• AccuGENX-ID® identification process is cGMP-compliant

• AccuGENX-ID® sequence-based approach is more reliable than traditional ID methodologies

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askcharlesriver@crl.com • www.criver.com © 2018, Charles River Laboratories International, Inc.

Having a breadth of coverage of bacterial species is critical for a high rate of identification and the accuracy of those

identifications. By prioritizing the entry into the reference library of species that are being encountered in manufacturing

facilities, the Accugenix® databases are the most relevant in the industry. The Frequency of Occurrence data below shows

the volume and diversity of organisms identified in our laboratories over a two year period and guides further library

development based on new and recurring isolates found in manufacturing environments around the world.

Protein Coding Gene Sequencing with AccuGENX-ID® ProSeqIdentification with ProSeq is a highly discriminatory and accurate sequence-based method that can be used to distinguish

between very closely related microorganisms to the species level. Resolution is achieved by analyzing protein coding genes.

If the AccuGENX-ID® BacSeq report contains a species* confidence level (indicating the identification resolution is to the

group or complex level), and we have determined the target gene that can distinguish between the different species in that

group of organisms, customers can request our identification with ProSeq service.

Eligible species include Burkholderia cepacia complex (BCC), among others. Please contact us for a full list of available

closely related microorganism groups for species-level identification.

Attributes of the AccuGENX-ID® microbial identification sequencing service:

• Reconciles effects of polymorphic positions (mixed bases and insertions/deletions)

• Utilizes full sequence potential (no truncation)

• Calculates distance measurements similarly to the genome method

• Considers both the distance measure and the phylogenetic tree in obtaining an identification

• Continuous maintenance to validated library

1

10

100

1000

10000

1

251

501

751

1001

1251

1501

1751

2001

2251

2501

2751

3001

3251

3501

3751

4001

Bacterial Species Diversity Jan 2016 - Feb 2018

4028 species comprising 236,000 samples

Species count

Freq

uenc

y of

Occ

urre

nce

(Log

)

Service Code

AccuGENX-ID® bacteria (16S 500bp)

Same day BacSeq-0

1 day BacSeq-1

2 day BacSeq-2

5 day BacSeq-5

AccuGENX-ID® ProSeq1 day PROSEQ-1

5 day PROSEQ-5

Turnaround Time