Usingopensource technologies todevelopROVpayloadstoobserveandcharacterise themarineenvironment.

*Teague.J1,Graham.G2,Allen.M.J31MResAppliedMarineScience,PlymouthUniversity,Pl48AA,2SirAlister HardyFoundationforOceanScience(SAHFOS),CitadelHill,PL12PB, 3 PlymouthMarineLaboratory

(PML),ProspectPlace,Plymouth,Plymouth,PL13DH

References: Blueplanet:seasoflife,television program,BritishBroadcastingCorporation(BBC),London, firstbroadcast:(12Sep2001).Census ofMarineLife.(2015).Niskin Bottles.[ONLINE]Availableat:http://www.coml.org/edu/tech/collect/niskin.htm.[ Accessed:23August2015].MarineTechnologySociety.(2012).What isanROV?.[ONLINE]Availableat:http://www.rov.org/rov_overview.cfm.[Accessed: 22August2015].ROV.org.(2015).WhatisanROV?.[ONLINE]Availableat:http://www.rov.org/rov_overview.cfm.[Accessed: 08January2015].Teague,J.(2013).Producing andusinganROV(RemotelyOperatedVehicle)inbenthicvideosurveysaroundtheCornishcoast.FdSc MarineScience.FalmouthMarineSchool.

VersatilePlatform 3DPrinting

CustomRNAProfilingPayload

• RapidPrototyping• Research&Development• Replacementparts

• NewRevolutionaryTechnique• “Offtheshelfcomponents”• ‘Freeze’samplesinsitu

• Opensource• Economical• RapidDeployment

ARemotelyOperatedVehicle(ROV)isatetheredunderwaterrobotthatallowsthevehicle'soperatortoremaininasafeenvironmentwhiletheequipment(ROV)worksinthepotentiallyhazardousenvironmentbelow.Theopensource ROV,(OpenROV),offersaversatilemechanismformaritimesurveying.

TheanalysisofnucleicacidmaterialfrommarinesystemsiscurrentlycommonlyachievedviaNiskin samplingandpreservationofnucleicacidsisdelayeduntilarrivalatthesurface.ThisprojectaimstodevelopaninsitunucleicacidsamplerwhichpreservesthepayloadandthereforexpandsthecapabilityoftheOpenROV platform.

Focuswillbeonthecollectionofmarinebiomassfromseawaterandmetabolically‘freezing’theRNAprofileinsitu topreventdegradationandstress-induceddevianceuponbringingthesampletothesurface,withinasimpleandcheapdeviceattachedtoanROV.WewillaimtousethisdevicetomonitormicrobialcommunitiesinrealtimeusingbasicmicroscopylighttoselectivelytargetSpecificwaterbodiesforsampling.

ThenucleicacidpreservationdevicewillbeconstructedusingvariouscomponentssuchasArduinoandRaspberrypiforprogrammablecontrolandaperistalticpumpforsamplingcoupledwithsensorsmonitoringdepth,temperatureandsalinity.

Abstract:

ROVPayloads• VastrangeofPayloads• AlreadydevelopedaNiskin fortheROV• InsituMicroscopyfor‘Targeted’sampling