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US AGENCI FOR
1-n N~noAL ec tiol
Dr Nathaniel Pugh Fort Valley State College 1005 State College Drive Fort valley GA 31030-3298 Subject Grant No PCE-5053-G-00-3045-00
Dear Dr Pugh
Pursuant to the authority contained in the Foreign Assistance
1961 and the Federal Grant and Cooperative Agreement ActAct of amended tie Agency for International Developmentof 1982 as
as(hereinafter referred to AID) hereby grants to Fort
Valley State College (hereinfter referred to as FVSC or the
the sum of $100000 to provide financial support forGrantee) the program described ii Attachment 2 of this Grant entitled
Program Description
as of the date ofThis Grant is effective and obligation is made
this letter and shall apply to expenditures made by the Grantee
in furtherance of program objectives during the period beginning 1995with the effective date and ending December 31
This Grant is made to the Grantee on the condition that the terms andfunds will be administered in accordance with the
as set forth in Attachment 1 (the Schedule)conditions Attachment 2 (the Program Description) and Attachment 3 (the
all of which have been agreed to by yourStandard Provisions) organization
Please sign the original and all enclosed copies of this letter
to acknowledge your receipt of this grant and return the one copy to the undersignedoriginal and all but
If you have any questions please contact Ms Karin Kolstrom of
my staff at (703) 875-1189
y
Sincere
Grant Officer Chief PCE Branch
Division B Office of Procurement
20523320 T~intfFIRST STREET NW WSHIGIONDC
PCE-5053-G-00-3045-00 Page 2
Attachments
i Schedule 2 Program Description
ACKNOWLEDGED
Fort Va ey Sta ollege
BY
TYPED NAME Oscar L Prater
TITLE President
DATE September 29 1993
96521
PCE-5053-G-00-3045-00
Page 3
FISCAL DATA
A GENERAL
A1 Total Estimated AID Amount $100000
A2 Total Obligated AID Amount $100000
A3 Cost-Sharing Amount (Non-Federal)$0 A4 Other Contributions (Federal) $ NA
A5 Project No936-5053 A6 AID Project Office RampDUC A7 Funding Source AIDW A8 Tax ID No 58-6002062 A9 CEC No NA A10 LOC No 72-00-
B SPECIFIC
B1(a) PIOT No 936-5053-3692948 Bl(b) Appropriation 72-11310211 B1(c) Allotment 341-36-099-00-20-31 B1(d) BPC DDVA-93-16900-KGll B1(e) Amount $100000
PCE-5053-G-00-3045-00 Page 4
ATTACHMENT 1
SCHEDULE
IA PURPOSE OF GRANT
The purpose of this Grant is to provide financial support for the program described in Attachment 2 of this Grant entitled Program Description
1B PERIOD OF GRANT
The effective date of this Grant is the date of the Cover Letter and the estimated completion date is December 31 1995 Funds obligated hereunder (see Section IC2 below) shall be used to reimburse the Grantee for allowable program expenditures incurred by the Grantee in pursuit of program objectives during such period Funds obligated hereunder are anticipated to be sufficient for completion by the Grantee of the program described in Attachment 2 of this Grant by the estimated completion date
IC AMOUNT OF GRANT AND PAYMENT
ICI The total estimated amount of this Grant for its full period as set forth in Section lB above is $100000
1C2 AID hereby obligates the amount of $100000 for the purposes of this Grant during the indicated period set forth in Section lB above thereby fulfilling AIDs funding requirements AID shall not be liable for reimbursing the Grantee for any costs in excess of the obligated amount except as specified in paragraph (f) of the Standard Provision of this Grant entitled Revision of Grant Budget
1C3 Payment shall be made to the Grantee in accordance with procedures set forth in the Standard Provision of this Grant entitled Payment - Periodic Advance as shown in Attachment 3
ID GRANT BUDGET
IDl The following is the Budget for the total estimated amount of this Grant (see Section ICl above) for its full period (see Section IB above) The Grantee may not exceed the total estimated amount or the obligated amount of this Grant whichever is less (see Sections ICI and 1C2 respectively above) Except as specified in the Standard Provision of this Grant entitled Revision of Grant Budget as shown in Attachment 3 the Grantee may adjust line item amounts as may be reasonably necessary for the attainment of program objectives
PCE-053-G-00-3045-00
Page 5
1D2 Bde Cost-Sharing
Cost Element AID N etal
Salaries $54200 $0 $54200
Fringe Travel
$4350 $18500
$0 $0
$4350 $18500
Non-Exp Equip $10500 ODC $8085 Overhead 64
$0 $0
$10500 $8085
365
Total $100000 $0 $100000
1D3 Inclusion of any cost in the budget of this Grant does
not obviate the requirement for prior approval by the Grant
Officer of cost items designated as requiring prior approval by
the applicable cost principles (see the Standard Provision of
this Grant set forth in Attachment 3 entitled Allowable Costs)
and other terms and conditions of this Grant unless
specifically stated in Section I below
1El Financial Reportina
IE1(a) Financial reporting requirements shall be in
accordance with the Standard Provision of this Grant entitled
Payment - Periodic Advance as shown in Attachment 3 If a
Standard Form 269 is required by the aforesaid Standard said form shall be usedProvision the Long Form of
iE1(b) All financial reports shall be submitted to
AID Office of Financial Management FAFMCMPDDCB Room
700 SA-2 Washington DC 20523-0209 In addition three
copies of all financial reports shall be submitted to the
AID Project Office specified in the Cover Letter of this
Grant concurrently with submission of the Quarterly Technical
Reports (See Section 1E2 below)
1El(c) The frequency of financial reporting and the due
dates of reports shall be as specified in the Standard in Section iEl(a) aboveProvision of this Grant referred to
1E2 Program Reporting
1E2(a) Annual Workplan
The Grantee shall submit an annual workplan for1E2(a)(1) this Grant which shall contain the following
1E2(a)()(A) An action-oriented workplan describing
planned activities for the next year delineated by calendar
PCE-5053-G-00-3045-00 Page 6
and objectives whichquarter and linked to the project goals bedescribes the individuals to be involved the activities to
conducted and where and when they will be conducted Planned
activities shall be grouped by subject category and then
related to project objectives
1E2(a)(1)(B) A projected budget utilizing the same as are set forth in the budget of this Grantbudget line items
for each calendar quarter corresponding to the workplan and
1E2(a)(1)(C) Publications reports workshops seminars
and other information dissemination activities planned by
calendar quarter
The Grantee may develop the annual workplan in1E2(a)(2) consultation with the AID Project Officer for this Grant
lE2(a)(3) Five (5) copies of the annual workplan shall be
submitted to the designated AID Project Officer for this
Grant and one copy submitted to the Grant Officer The annual
workplan shall be submitted by the Grantee not later than sixty
(60) days from the effective date of this Grant (see Section
lB above)
IE2(b) Ouarterly Reports
The Grantee shall submit five (5) copies of brief quarterly
program performance reports which coincide with the financial
reporting periods described in Section lEI above to the
AID Project Office specified in the Cover Letter of this
Grant In addition two copies shall be submitted to AID
POLCDIEDI Washington DC 20523-1802 These reports shall be
submitted within 30 days following the end of the reporting
period and shall briefly present the following information
1E2(b)(1) A comparison of actual accomplishments with the
goals established for the period the findings of the investigator or both If the output of programs can be
readily quantified such quantitative data should be related to
cost data for computation of unit costs
of finances and expenditures and when appropriate analysis
1E2(b)(2) Reasons why established goals were not met if
applicable
1E2(b)(3) Other pertinent information including the status
and explanation of cost overruns or high unit costs
IE2(c) Special Reports
1E2(c)(1) Within 30 days following the completion of each a tripinternational trip the Grantee shall submit 3 copies of
PCE-5053-G-00-3045-00 Page 7
report summarizing the accomplishments of the trip to the
AID Project Officer specified in the cover letter of this
Grant If several individuals are travelling together to one The
site a single report representing the group will suffice
report shall include the purpose of the trip technical
observations suggestions and recommendations overall
impressions of the site situation (if appropriate) and a list
of persons visited with their title and organization
affiliation
Between the required program performance1E2(c)(2) that have significant impact
reporting dates events may occur inform upon the program In such instances the Grantee shall
the AID Project Officer as soon as the following types of
conditions become known
adverse conditions thatIE2(c)(2)(A) Problems delays or
attain programwill materially affect the ability to
objectives prevent the meeting of time schedules and goals or
preclude the attainment of work units by established time
This disclosure shall be accompanied by a statementperiods of the action taken or contemplated and any AID assistance
needed to resolve the situation
Favorable developments or events that enableIE2(c)(2)(B) sooner than anticipated or more worktime schedules to be met
units to be produced than originally projected
lE2(c)(3) If any performance review conducted by the
Grantee discloses the need for change in the budget estimates
in accordance with the criteria established in the Standard
this Grant entitled Revision of Grant BudgetProvision of the Grantee shall submit a request for budget revision to
the
Grant Officer and the AID Project Officer specified in the
Cover Letter of this Grant
IE2(d) Environmental Impact
this project will result inIf it appears that the outputs of
an adverse environmental impact the Grantee shall notify the
AID Project Officer prior to implementation in order to
allow for orderly preparation of an environmental impact that appropriate USstatement The Grantee shall assure
followedGovernment andor host country procedures are
1E2(e) Care of Laboratory Animals
If the Standard Provision entitled Care of Laboratory Animals
(see Section for applicability) theapplies to this Grant Grantee shall include the certificate required by paragraph
(c)
of said Standard Provision in all of its reports which pertain
to the use of laboratory animals
PCE-5053-G-00-3045-00
Page 8
IE2(f) Final Report
Within 90 days following the estimated completion date of this Grant (see Section lB above) the Grantee shall submit five (5) copies of a final report to the AID Project Office specified in the cover letter of this Grant In addition two copies shall be submitted to AID POLCDIEDI Washington DC 20523-1802 It will cover the entire period of the Grant and include all information shown in Sections 1E2(a) and 1E2(c) above
IF SPECIAL PROVISIONS
1F1 OPTIONAL STANDARD PROVISIONS
The following Optional Standard Provisions for US Nongovernmental Grantees as listed in Attachment 3 of this Grant are hereby deleted as follows
Provision Payment - Periodic Advance 13 Payment - Cost Reimbursement 15 Local Cost Financing 33 Patent Rights 35 Negotiated Indirect C-t Rates - Provisional 46 Participant Training 48 Voluntary Population Planning 49 Protection of the Individual as a Research Subject 56 Title To and Care of Property(US Government Title) 63 Title To and Care of Property(Cooperating Country Title)67 Cost Sharing 71
All other Optional Standard Provisions are hereby incorporated into this Grant and have been checked off on page 9 of Attachment 3 IF2 Limitations on Reimbursement of Costs of Compensation
for Personal Services and Professional Service Costs
IF2(a) Employee Salaries
Except as the Grant Officer may otherwise agree in writing AID shall not be liable for reimbursing the Grantee for any costs allocable to the salary portion of direct compensation paid by the Grantee to its employees for personal services which exceed the highest salary level for a Foreign Service Officer Class 1 (FS-l) as periodically amended
IF2(b) Consultant Fees
Compensation for consultants retained by the Grantee hereunder shall not exceed without specific approval of the rate by the Grant Officer either the highest rate of annual compensation
P2E-5053-G-00-3045-00 Page 9
received by the consultant during any full year of the
or the maximum rate of aimmediately preceding three years
Foreign Service Officer Class 1 (FS-l) (as periodically A daily rate is derived byamended) whichever is less
dividing the annual compensation by 2087 and multiplying the
result by 8
1F3 Defense Base Act (DBA) and Medical Evacuation Insurance
Section J16 of OMB Circular A-21 (for educationalPursuant to Section 18 of Attachment B of OMB Circularinstitutions) or
A-122 (for nonprofit organizations other than educational
institutions) the Grantee is authorized to purchase DBA andor If DBA insurancemedical evacuation insurance under this Grant
is purchased it shall be purchased from the insurance company
agent with which AID has a contract to provide DBAor insurance for AID contracts The Grant Officer will provide
the name address and telephone number of such insurance
company or agent upon request
IG INDIRECT COST RATE
IGI Pursuant to the Standard Provision of this Grant
entitled Negotiated Indirect Cost Rates - Provisional
(Nonprofits) and if applicable Negotiated Indirect Cost or rates shall beRates - Predetermined an indirect cost rate
the Grantees accounting periods whichestablished for each of this Grant Payment on account of allowable indirectapply to
on the basis of final or predeterminedcosts shall be made indirect cost rates for each accounting period which applies to
this Grant but not in excess of the following ceiling rate(s) applied to the base(s) which is (are) set forth below Any indirect costs above the ceiling rate(s) shall be absorbed by the Grantee and considered cost sharing
Rate Rase
On-CampusHome Office 520 1 Off-CampusOff-Site 291 1
J Base of Application direct salaries including vacation holiday sick pay and other paid absences but excluding all other fringe benefits
1H PROPERTY REQUIREMENTS
1HI Title to Property
Title to property acquired hereunder shall vest in the Grantee subject to the requirements of the Standard Provision of this grant entitled Title To and Use of Property (Grantee Title)
regarding use accountability and disposition of such property
(7
PCE-5053-G-00-3045-00
Page 10
1H2 Ecuipment Purchases
Equipment purchases under this Grant must be made in accordance
with the Standard Provisions entitled Procurement of Goods and
Services and AID Eligibility Rules for Goods and Services
included in Attachment 3 of this Grant Inclusion of costs in
the budget of this Grant for the purchase of nonexpendable equipment does not obviate the requirements of Section J13 of
OMB Circular A-21 (for educational institutions) or Section 13
of Attachment B of OMB Circular A-122 (for nonprofit organizations other than educational institutions) for prior
approval of such purchases by the Grant Officer nor any other
terms and conditions of this Grant unless specifically stated
in Section lH2(a)(3) below
IH2(a) Reguirement for Prior Approval
Pursuant to Sections 1D3 and 1G3 above and the Standard
Provisions of this Grant entitled Allowable Costs and
Revision of Grant Budget and by extension Section 13 of
Attachment B of OMB Circular A-122 the Grantee must obtain AID Grant Officer approval for purchases of the following
1H2(a)(1) General Purpose EQuipment which is defined as an
article of nonexpendable tangible personal property which is
usable for other than research medical scientific or technical
activities whether or not special modifications are needed to
make them suitable for a particular purpose (eg office
equipment and furnishings air conditioning equipment reproduction and printing equipment motor vehicles and
automatic data processing equipment) having a useful life of
more than two years and an acquisition cost of $500 or more per unit) and
IH2(a)(2) Special Purpose EguiPment which is defined as an
article of nonexpendable tangible personal property which is used only for research medical scientific or technical activities (eg microscopes x-ray machines surgical instruments and spectrometers) and which has a useful life of
more than two years and an acquisition cost of $1000 or more per unit)
1H2(a)(3) Approvals
In furtherance of the foregoing the Grant Officer does hereby provide approval for the following purchases which shall not be
construed as authorization to exceed the total estimated amount
or the obligated amount of this Grant whichever is less (see
Section 1C above)
- one microscope - two laboratory refrigerators
6)
PCE-5053-G-00-3045-00 Page 11
- two laboratoryfreezers - one diesel engine
one water pump and associated electrical materials-
IH2(a)(4) EMueption for Automation Eguipment
for the purchase of automation equipment which mayAny approval be provided in Section IH2(a)(3) above or subsequently
is not valid if the total cost of provided by the Grant Officer
purchases of automation equipment (eg computers word
related services made hereunder processors etc) software or
will exceed $100000 The Grantee must under such the Grant Officer for the
circumstances obtain the approval of any automation equipment software or total planned system of
related services
11 RESOLUTION OF CONFLICTS
the Attachments of this Grant shall beConflicts between any of
resolved by applying the following descending order of
precedence
Attachment 1 - Schedule - Standard ProvisionsAttachment 3
Attachment 2 - Program Description
Agency For International Development Research Grant Program
for Historically Black Colleges and Universities
Proposal Title Development and Dissemination of True Potato Seed Technology in Uganda
Institution Fort Valley State College Fort Valley Georgia 31030
Principal Investigator Dr A S Bhagsari Professor
AGENCY FOR INTERNATIONAL DEVELOPMENT RESEARCH GRANT PROGRAM
FOR HISTORICAL BLACK COLLEGES AND UNIVERSITIES
SUBMITTED BY FORT VALLEY STATE COLLEGE (FVSC) Agricultural Research Station 1005 State College Drive Fort Valley Georgia 31030-3298
PROPOSAL TITLE DEVELOPMENT AND DISSEMINATION OF TRUE POTATO SEED TECHNOLOGY IN UGANDA
PRINCIPAL INVESTIGATOR Ajmer SBhagsari Ph D (USA) Professor
Agricultural Research Station Fort Valley State College Fort Valley Georgia USA Telephone No (912) 825-6825 Fax No (912) 825-6376
CO-INVESTIGATOR Adipala Ekwamu PhD (Uganda) Faculty of Agriculture and Forestry
Department of Crop Science Makerere University Kampala Uganda Africa
DATE OF SUBMISSION April 1993
Signature of Principal Investigator Sgnature an Name
Agricultural Research Station Representative of Proposing Insfitution
Statements on Proposal Submission
This project proposal in part or in whole has not been submitted to any other1 funding agency and is not closely related to research being done for other sponsors
2 This project if approved will provide for its own logistic support and will not pose
any load on the USAID mission
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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23
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24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
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2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
PCE-5053-G-00-3045-00 Page 2
Attachments
i Schedule 2 Program Description
ACKNOWLEDGED
Fort Va ey Sta ollege
BY
TYPED NAME Oscar L Prater
TITLE President
DATE September 29 1993
96521
PCE-5053-G-00-3045-00
Page 3
FISCAL DATA
A GENERAL
A1 Total Estimated AID Amount $100000
A2 Total Obligated AID Amount $100000
A3 Cost-Sharing Amount (Non-Federal)$0 A4 Other Contributions (Federal) $ NA
A5 Project No936-5053 A6 AID Project Office RampDUC A7 Funding Source AIDW A8 Tax ID No 58-6002062 A9 CEC No NA A10 LOC No 72-00-
B SPECIFIC
B1(a) PIOT No 936-5053-3692948 Bl(b) Appropriation 72-11310211 B1(c) Allotment 341-36-099-00-20-31 B1(d) BPC DDVA-93-16900-KGll B1(e) Amount $100000
PCE-5053-G-00-3045-00 Page 4
ATTACHMENT 1
SCHEDULE
IA PURPOSE OF GRANT
The purpose of this Grant is to provide financial support for the program described in Attachment 2 of this Grant entitled Program Description
1B PERIOD OF GRANT
The effective date of this Grant is the date of the Cover Letter and the estimated completion date is December 31 1995 Funds obligated hereunder (see Section IC2 below) shall be used to reimburse the Grantee for allowable program expenditures incurred by the Grantee in pursuit of program objectives during such period Funds obligated hereunder are anticipated to be sufficient for completion by the Grantee of the program described in Attachment 2 of this Grant by the estimated completion date
IC AMOUNT OF GRANT AND PAYMENT
ICI The total estimated amount of this Grant for its full period as set forth in Section lB above is $100000
1C2 AID hereby obligates the amount of $100000 for the purposes of this Grant during the indicated period set forth in Section lB above thereby fulfilling AIDs funding requirements AID shall not be liable for reimbursing the Grantee for any costs in excess of the obligated amount except as specified in paragraph (f) of the Standard Provision of this Grant entitled Revision of Grant Budget
1C3 Payment shall be made to the Grantee in accordance with procedures set forth in the Standard Provision of this Grant entitled Payment - Periodic Advance as shown in Attachment 3
ID GRANT BUDGET
IDl The following is the Budget for the total estimated amount of this Grant (see Section ICl above) for its full period (see Section IB above) The Grantee may not exceed the total estimated amount or the obligated amount of this Grant whichever is less (see Sections ICI and 1C2 respectively above) Except as specified in the Standard Provision of this Grant entitled Revision of Grant Budget as shown in Attachment 3 the Grantee may adjust line item amounts as may be reasonably necessary for the attainment of program objectives
PCE-053-G-00-3045-00
Page 5
1D2 Bde Cost-Sharing
Cost Element AID N etal
Salaries $54200 $0 $54200
Fringe Travel
$4350 $18500
$0 $0
$4350 $18500
Non-Exp Equip $10500 ODC $8085 Overhead 64
$0 $0
$10500 $8085
365
Total $100000 $0 $100000
1D3 Inclusion of any cost in the budget of this Grant does
not obviate the requirement for prior approval by the Grant
Officer of cost items designated as requiring prior approval by
the applicable cost principles (see the Standard Provision of
this Grant set forth in Attachment 3 entitled Allowable Costs)
and other terms and conditions of this Grant unless
specifically stated in Section I below
1El Financial Reportina
IE1(a) Financial reporting requirements shall be in
accordance with the Standard Provision of this Grant entitled
Payment - Periodic Advance as shown in Attachment 3 If a
Standard Form 269 is required by the aforesaid Standard said form shall be usedProvision the Long Form of
iE1(b) All financial reports shall be submitted to
AID Office of Financial Management FAFMCMPDDCB Room
700 SA-2 Washington DC 20523-0209 In addition three
copies of all financial reports shall be submitted to the
AID Project Office specified in the Cover Letter of this
Grant concurrently with submission of the Quarterly Technical
Reports (See Section 1E2 below)
1El(c) The frequency of financial reporting and the due
dates of reports shall be as specified in the Standard in Section iEl(a) aboveProvision of this Grant referred to
1E2 Program Reporting
1E2(a) Annual Workplan
The Grantee shall submit an annual workplan for1E2(a)(1) this Grant which shall contain the following
1E2(a)()(A) An action-oriented workplan describing
planned activities for the next year delineated by calendar
PCE-5053-G-00-3045-00 Page 6
and objectives whichquarter and linked to the project goals bedescribes the individuals to be involved the activities to
conducted and where and when they will be conducted Planned
activities shall be grouped by subject category and then
related to project objectives
1E2(a)(1)(B) A projected budget utilizing the same as are set forth in the budget of this Grantbudget line items
for each calendar quarter corresponding to the workplan and
1E2(a)(1)(C) Publications reports workshops seminars
and other information dissemination activities planned by
calendar quarter
The Grantee may develop the annual workplan in1E2(a)(2) consultation with the AID Project Officer for this Grant
lE2(a)(3) Five (5) copies of the annual workplan shall be
submitted to the designated AID Project Officer for this
Grant and one copy submitted to the Grant Officer The annual
workplan shall be submitted by the Grantee not later than sixty
(60) days from the effective date of this Grant (see Section
lB above)
IE2(b) Ouarterly Reports
The Grantee shall submit five (5) copies of brief quarterly
program performance reports which coincide with the financial
reporting periods described in Section lEI above to the
AID Project Office specified in the Cover Letter of this
Grant In addition two copies shall be submitted to AID
POLCDIEDI Washington DC 20523-1802 These reports shall be
submitted within 30 days following the end of the reporting
period and shall briefly present the following information
1E2(b)(1) A comparison of actual accomplishments with the
goals established for the period the findings of the investigator or both If the output of programs can be
readily quantified such quantitative data should be related to
cost data for computation of unit costs
of finances and expenditures and when appropriate analysis
1E2(b)(2) Reasons why established goals were not met if
applicable
1E2(b)(3) Other pertinent information including the status
and explanation of cost overruns or high unit costs
IE2(c) Special Reports
1E2(c)(1) Within 30 days following the completion of each a tripinternational trip the Grantee shall submit 3 copies of
PCE-5053-G-00-3045-00 Page 7
report summarizing the accomplishments of the trip to the
AID Project Officer specified in the cover letter of this
Grant If several individuals are travelling together to one The
site a single report representing the group will suffice
report shall include the purpose of the trip technical
observations suggestions and recommendations overall
impressions of the site situation (if appropriate) and a list
of persons visited with their title and organization
affiliation
Between the required program performance1E2(c)(2) that have significant impact
reporting dates events may occur inform upon the program In such instances the Grantee shall
the AID Project Officer as soon as the following types of
conditions become known
adverse conditions thatIE2(c)(2)(A) Problems delays or
attain programwill materially affect the ability to
objectives prevent the meeting of time schedules and goals or
preclude the attainment of work units by established time
This disclosure shall be accompanied by a statementperiods of the action taken or contemplated and any AID assistance
needed to resolve the situation
Favorable developments or events that enableIE2(c)(2)(B) sooner than anticipated or more worktime schedules to be met
units to be produced than originally projected
lE2(c)(3) If any performance review conducted by the
Grantee discloses the need for change in the budget estimates
in accordance with the criteria established in the Standard
this Grant entitled Revision of Grant BudgetProvision of the Grantee shall submit a request for budget revision to
the
Grant Officer and the AID Project Officer specified in the
Cover Letter of this Grant
IE2(d) Environmental Impact
this project will result inIf it appears that the outputs of
an adverse environmental impact the Grantee shall notify the
AID Project Officer prior to implementation in order to
allow for orderly preparation of an environmental impact that appropriate USstatement The Grantee shall assure
followedGovernment andor host country procedures are
1E2(e) Care of Laboratory Animals
If the Standard Provision entitled Care of Laboratory Animals
(see Section for applicability) theapplies to this Grant Grantee shall include the certificate required by paragraph
(c)
of said Standard Provision in all of its reports which pertain
to the use of laboratory animals
PCE-5053-G-00-3045-00
Page 8
IE2(f) Final Report
Within 90 days following the estimated completion date of this Grant (see Section lB above) the Grantee shall submit five (5) copies of a final report to the AID Project Office specified in the cover letter of this Grant In addition two copies shall be submitted to AID POLCDIEDI Washington DC 20523-1802 It will cover the entire period of the Grant and include all information shown in Sections 1E2(a) and 1E2(c) above
IF SPECIAL PROVISIONS
1F1 OPTIONAL STANDARD PROVISIONS
The following Optional Standard Provisions for US Nongovernmental Grantees as listed in Attachment 3 of this Grant are hereby deleted as follows
Provision Payment - Periodic Advance 13 Payment - Cost Reimbursement 15 Local Cost Financing 33 Patent Rights 35 Negotiated Indirect C-t Rates - Provisional 46 Participant Training 48 Voluntary Population Planning 49 Protection of the Individual as a Research Subject 56 Title To and Care of Property(US Government Title) 63 Title To and Care of Property(Cooperating Country Title)67 Cost Sharing 71
All other Optional Standard Provisions are hereby incorporated into this Grant and have been checked off on page 9 of Attachment 3 IF2 Limitations on Reimbursement of Costs of Compensation
for Personal Services and Professional Service Costs
IF2(a) Employee Salaries
Except as the Grant Officer may otherwise agree in writing AID shall not be liable for reimbursing the Grantee for any costs allocable to the salary portion of direct compensation paid by the Grantee to its employees for personal services which exceed the highest salary level for a Foreign Service Officer Class 1 (FS-l) as periodically amended
IF2(b) Consultant Fees
Compensation for consultants retained by the Grantee hereunder shall not exceed without specific approval of the rate by the Grant Officer either the highest rate of annual compensation
P2E-5053-G-00-3045-00 Page 9
received by the consultant during any full year of the
or the maximum rate of aimmediately preceding three years
Foreign Service Officer Class 1 (FS-l) (as periodically A daily rate is derived byamended) whichever is less
dividing the annual compensation by 2087 and multiplying the
result by 8
1F3 Defense Base Act (DBA) and Medical Evacuation Insurance
Section J16 of OMB Circular A-21 (for educationalPursuant to Section 18 of Attachment B of OMB Circularinstitutions) or
A-122 (for nonprofit organizations other than educational
institutions) the Grantee is authorized to purchase DBA andor If DBA insurancemedical evacuation insurance under this Grant
is purchased it shall be purchased from the insurance company
agent with which AID has a contract to provide DBAor insurance for AID contracts The Grant Officer will provide
the name address and telephone number of such insurance
company or agent upon request
IG INDIRECT COST RATE
IGI Pursuant to the Standard Provision of this Grant
entitled Negotiated Indirect Cost Rates - Provisional
(Nonprofits) and if applicable Negotiated Indirect Cost or rates shall beRates - Predetermined an indirect cost rate
the Grantees accounting periods whichestablished for each of this Grant Payment on account of allowable indirectapply to
on the basis of final or predeterminedcosts shall be made indirect cost rates for each accounting period which applies to
this Grant but not in excess of the following ceiling rate(s) applied to the base(s) which is (are) set forth below Any indirect costs above the ceiling rate(s) shall be absorbed by the Grantee and considered cost sharing
Rate Rase
On-CampusHome Office 520 1 Off-CampusOff-Site 291 1
J Base of Application direct salaries including vacation holiday sick pay and other paid absences but excluding all other fringe benefits
1H PROPERTY REQUIREMENTS
1HI Title to Property
Title to property acquired hereunder shall vest in the Grantee subject to the requirements of the Standard Provision of this grant entitled Title To and Use of Property (Grantee Title)
regarding use accountability and disposition of such property
(7
PCE-5053-G-00-3045-00
Page 10
1H2 Ecuipment Purchases
Equipment purchases under this Grant must be made in accordance
with the Standard Provisions entitled Procurement of Goods and
Services and AID Eligibility Rules for Goods and Services
included in Attachment 3 of this Grant Inclusion of costs in
the budget of this Grant for the purchase of nonexpendable equipment does not obviate the requirements of Section J13 of
OMB Circular A-21 (for educational institutions) or Section 13
of Attachment B of OMB Circular A-122 (for nonprofit organizations other than educational institutions) for prior
approval of such purchases by the Grant Officer nor any other
terms and conditions of this Grant unless specifically stated
in Section lH2(a)(3) below
IH2(a) Reguirement for Prior Approval
Pursuant to Sections 1D3 and 1G3 above and the Standard
Provisions of this Grant entitled Allowable Costs and
Revision of Grant Budget and by extension Section 13 of
Attachment B of OMB Circular A-122 the Grantee must obtain AID Grant Officer approval for purchases of the following
1H2(a)(1) General Purpose EQuipment which is defined as an
article of nonexpendable tangible personal property which is
usable for other than research medical scientific or technical
activities whether or not special modifications are needed to
make them suitable for a particular purpose (eg office
equipment and furnishings air conditioning equipment reproduction and printing equipment motor vehicles and
automatic data processing equipment) having a useful life of
more than two years and an acquisition cost of $500 or more per unit) and
IH2(a)(2) Special Purpose EguiPment which is defined as an
article of nonexpendable tangible personal property which is used only for research medical scientific or technical activities (eg microscopes x-ray machines surgical instruments and spectrometers) and which has a useful life of
more than two years and an acquisition cost of $1000 or more per unit)
1H2(a)(3) Approvals
In furtherance of the foregoing the Grant Officer does hereby provide approval for the following purchases which shall not be
construed as authorization to exceed the total estimated amount
or the obligated amount of this Grant whichever is less (see
Section 1C above)
- one microscope - two laboratory refrigerators
6)
PCE-5053-G-00-3045-00 Page 11
- two laboratoryfreezers - one diesel engine
one water pump and associated electrical materials-
IH2(a)(4) EMueption for Automation Eguipment
for the purchase of automation equipment which mayAny approval be provided in Section IH2(a)(3) above or subsequently
is not valid if the total cost of provided by the Grant Officer
purchases of automation equipment (eg computers word
related services made hereunder processors etc) software or
will exceed $100000 The Grantee must under such the Grant Officer for the
circumstances obtain the approval of any automation equipment software or total planned system of
related services
11 RESOLUTION OF CONFLICTS
the Attachments of this Grant shall beConflicts between any of
resolved by applying the following descending order of
precedence
Attachment 1 - Schedule - Standard ProvisionsAttachment 3
Attachment 2 - Program Description
Agency For International Development Research Grant Program
for Historically Black Colleges and Universities
Proposal Title Development and Dissemination of True Potato Seed Technology in Uganda
Institution Fort Valley State College Fort Valley Georgia 31030
Principal Investigator Dr A S Bhagsari Professor
AGENCY FOR INTERNATIONAL DEVELOPMENT RESEARCH GRANT PROGRAM
FOR HISTORICAL BLACK COLLEGES AND UNIVERSITIES
SUBMITTED BY FORT VALLEY STATE COLLEGE (FVSC) Agricultural Research Station 1005 State College Drive Fort Valley Georgia 31030-3298
PROPOSAL TITLE DEVELOPMENT AND DISSEMINATION OF TRUE POTATO SEED TECHNOLOGY IN UGANDA
PRINCIPAL INVESTIGATOR Ajmer SBhagsari Ph D (USA) Professor
Agricultural Research Station Fort Valley State College Fort Valley Georgia USA Telephone No (912) 825-6825 Fax No (912) 825-6376
CO-INVESTIGATOR Adipala Ekwamu PhD (Uganda) Faculty of Agriculture and Forestry
Department of Crop Science Makerere University Kampala Uganda Africa
DATE OF SUBMISSION April 1993
Signature of Principal Investigator Sgnature an Name
Agricultural Research Station Representative of Proposing Insfitution
Statements on Proposal Submission
This project proposal in part or in whole has not been submitted to any other1 funding agency and is not closely related to research being done for other sponsors
2 This project if approved will provide for its own logistic support and will not pose
any load on the USAID mission
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
PCE-5053-G-00-3045-00
Page 3
FISCAL DATA
A GENERAL
A1 Total Estimated AID Amount $100000
A2 Total Obligated AID Amount $100000
A3 Cost-Sharing Amount (Non-Federal)$0 A4 Other Contributions (Federal) $ NA
A5 Project No936-5053 A6 AID Project Office RampDUC A7 Funding Source AIDW A8 Tax ID No 58-6002062 A9 CEC No NA A10 LOC No 72-00-
B SPECIFIC
B1(a) PIOT No 936-5053-3692948 Bl(b) Appropriation 72-11310211 B1(c) Allotment 341-36-099-00-20-31 B1(d) BPC DDVA-93-16900-KGll B1(e) Amount $100000
PCE-5053-G-00-3045-00 Page 4
ATTACHMENT 1
SCHEDULE
IA PURPOSE OF GRANT
The purpose of this Grant is to provide financial support for the program described in Attachment 2 of this Grant entitled Program Description
1B PERIOD OF GRANT
The effective date of this Grant is the date of the Cover Letter and the estimated completion date is December 31 1995 Funds obligated hereunder (see Section IC2 below) shall be used to reimburse the Grantee for allowable program expenditures incurred by the Grantee in pursuit of program objectives during such period Funds obligated hereunder are anticipated to be sufficient for completion by the Grantee of the program described in Attachment 2 of this Grant by the estimated completion date
IC AMOUNT OF GRANT AND PAYMENT
ICI The total estimated amount of this Grant for its full period as set forth in Section lB above is $100000
1C2 AID hereby obligates the amount of $100000 for the purposes of this Grant during the indicated period set forth in Section lB above thereby fulfilling AIDs funding requirements AID shall not be liable for reimbursing the Grantee for any costs in excess of the obligated amount except as specified in paragraph (f) of the Standard Provision of this Grant entitled Revision of Grant Budget
1C3 Payment shall be made to the Grantee in accordance with procedures set forth in the Standard Provision of this Grant entitled Payment - Periodic Advance as shown in Attachment 3
ID GRANT BUDGET
IDl The following is the Budget for the total estimated amount of this Grant (see Section ICl above) for its full period (see Section IB above) The Grantee may not exceed the total estimated amount or the obligated amount of this Grant whichever is less (see Sections ICI and 1C2 respectively above) Except as specified in the Standard Provision of this Grant entitled Revision of Grant Budget as shown in Attachment 3 the Grantee may adjust line item amounts as may be reasonably necessary for the attainment of program objectives
PCE-053-G-00-3045-00
Page 5
1D2 Bde Cost-Sharing
Cost Element AID N etal
Salaries $54200 $0 $54200
Fringe Travel
$4350 $18500
$0 $0
$4350 $18500
Non-Exp Equip $10500 ODC $8085 Overhead 64
$0 $0
$10500 $8085
365
Total $100000 $0 $100000
1D3 Inclusion of any cost in the budget of this Grant does
not obviate the requirement for prior approval by the Grant
Officer of cost items designated as requiring prior approval by
the applicable cost principles (see the Standard Provision of
this Grant set forth in Attachment 3 entitled Allowable Costs)
and other terms and conditions of this Grant unless
specifically stated in Section I below
1El Financial Reportina
IE1(a) Financial reporting requirements shall be in
accordance with the Standard Provision of this Grant entitled
Payment - Periodic Advance as shown in Attachment 3 If a
Standard Form 269 is required by the aforesaid Standard said form shall be usedProvision the Long Form of
iE1(b) All financial reports shall be submitted to
AID Office of Financial Management FAFMCMPDDCB Room
700 SA-2 Washington DC 20523-0209 In addition three
copies of all financial reports shall be submitted to the
AID Project Office specified in the Cover Letter of this
Grant concurrently with submission of the Quarterly Technical
Reports (See Section 1E2 below)
1El(c) The frequency of financial reporting and the due
dates of reports shall be as specified in the Standard in Section iEl(a) aboveProvision of this Grant referred to
1E2 Program Reporting
1E2(a) Annual Workplan
The Grantee shall submit an annual workplan for1E2(a)(1) this Grant which shall contain the following
1E2(a)()(A) An action-oriented workplan describing
planned activities for the next year delineated by calendar
PCE-5053-G-00-3045-00 Page 6
and objectives whichquarter and linked to the project goals bedescribes the individuals to be involved the activities to
conducted and where and when they will be conducted Planned
activities shall be grouped by subject category and then
related to project objectives
1E2(a)(1)(B) A projected budget utilizing the same as are set forth in the budget of this Grantbudget line items
for each calendar quarter corresponding to the workplan and
1E2(a)(1)(C) Publications reports workshops seminars
and other information dissemination activities planned by
calendar quarter
The Grantee may develop the annual workplan in1E2(a)(2) consultation with the AID Project Officer for this Grant
lE2(a)(3) Five (5) copies of the annual workplan shall be
submitted to the designated AID Project Officer for this
Grant and one copy submitted to the Grant Officer The annual
workplan shall be submitted by the Grantee not later than sixty
(60) days from the effective date of this Grant (see Section
lB above)
IE2(b) Ouarterly Reports
The Grantee shall submit five (5) copies of brief quarterly
program performance reports which coincide with the financial
reporting periods described in Section lEI above to the
AID Project Office specified in the Cover Letter of this
Grant In addition two copies shall be submitted to AID
POLCDIEDI Washington DC 20523-1802 These reports shall be
submitted within 30 days following the end of the reporting
period and shall briefly present the following information
1E2(b)(1) A comparison of actual accomplishments with the
goals established for the period the findings of the investigator or both If the output of programs can be
readily quantified such quantitative data should be related to
cost data for computation of unit costs
of finances and expenditures and when appropriate analysis
1E2(b)(2) Reasons why established goals were not met if
applicable
1E2(b)(3) Other pertinent information including the status
and explanation of cost overruns or high unit costs
IE2(c) Special Reports
1E2(c)(1) Within 30 days following the completion of each a tripinternational trip the Grantee shall submit 3 copies of
PCE-5053-G-00-3045-00 Page 7
report summarizing the accomplishments of the trip to the
AID Project Officer specified in the cover letter of this
Grant If several individuals are travelling together to one The
site a single report representing the group will suffice
report shall include the purpose of the trip technical
observations suggestions and recommendations overall
impressions of the site situation (if appropriate) and a list
of persons visited with their title and organization
affiliation
Between the required program performance1E2(c)(2) that have significant impact
reporting dates events may occur inform upon the program In such instances the Grantee shall
the AID Project Officer as soon as the following types of
conditions become known
adverse conditions thatIE2(c)(2)(A) Problems delays or
attain programwill materially affect the ability to
objectives prevent the meeting of time schedules and goals or
preclude the attainment of work units by established time
This disclosure shall be accompanied by a statementperiods of the action taken or contemplated and any AID assistance
needed to resolve the situation
Favorable developments or events that enableIE2(c)(2)(B) sooner than anticipated or more worktime schedules to be met
units to be produced than originally projected
lE2(c)(3) If any performance review conducted by the
Grantee discloses the need for change in the budget estimates
in accordance with the criteria established in the Standard
this Grant entitled Revision of Grant BudgetProvision of the Grantee shall submit a request for budget revision to
the
Grant Officer and the AID Project Officer specified in the
Cover Letter of this Grant
IE2(d) Environmental Impact
this project will result inIf it appears that the outputs of
an adverse environmental impact the Grantee shall notify the
AID Project Officer prior to implementation in order to
allow for orderly preparation of an environmental impact that appropriate USstatement The Grantee shall assure
followedGovernment andor host country procedures are
1E2(e) Care of Laboratory Animals
If the Standard Provision entitled Care of Laboratory Animals
(see Section for applicability) theapplies to this Grant Grantee shall include the certificate required by paragraph
(c)
of said Standard Provision in all of its reports which pertain
to the use of laboratory animals
PCE-5053-G-00-3045-00
Page 8
IE2(f) Final Report
Within 90 days following the estimated completion date of this Grant (see Section lB above) the Grantee shall submit five (5) copies of a final report to the AID Project Office specified in the cover letter of this Grant In addition two copies shall be submitted to AID POLCDIEDI Washington DC 20523-1802 It will cover the entire period of the Grant and include all information shown in Sections 1E2(a) and 1E2(c) above
IF SPECIAL PROVISIONS
1F1 OPTIONAL STANDARD PROVISIONS
The following Optional Standard Provisions for US Nongovernmental Grantees as listed in Attachment 3 of this Grant are hereby deleted as follows
Provision Payment - Periodic Advance 13 Payment - Cost Reimbursement 15 Local Cost Financing 33 Patent Rights 35 Negotiated Indirect C-t Rates - Provisional 46 Participant Training 48 Voluntary Population Planning 49 Protection of the Individual as a Research Subject 56 Title To and Care of Property(US Government Title) 63 Title To and Care of Property(Cooperating Country Title)67 Cost Sharing 71
All other Optional Standard Provisions are hereby incorporated into this Grant and have been checked off on page 9 of Attachment 3 IF2 Limitations on Reimbursement of Costs of Compensation
for Personal Services and Professional Service Costs
IF2(a) Employee Salaries
Except as the Grant Officer may otherwise agree in writing AID shall not be liable for reimbursing the Grantee for any costs allocable to the salary portion of direct compensation paid by the Grantee to its employees for personal services which exceed the highest salary level for a Foreign Service Officer Class 1 (FS-l) as periodically amended
IF2(b) Consultant Fees
Compensation for consultants retained by the Grantee hereunder shall not exceed without specific approval of the rate by the Grant Officer either the highest rate of annual compensation
P2E-5053-G-00-3045-00 Page 9
received by the consultant during any full year of the
or the maximum rate of aimmediately preceding three years
Foreign Service Officer Class 1 (FS-l) (as periodically A daily rate is derived byamended) whichever is less
dividing the annual compensation by 2087 and multiplying the
result by 8
1F3 Defense Base Act (DBA) and Medical Evacuation Insurance
Section J16 of OMB Circular A-21 (for educationalPursuant to Section 18 of Attachment B of OMB Circularinstitutions) or
A-122 (for nonprofit organizations other than educational
institutions) the Grantee is authorized to purchase DBA andor If DBA insurancemedical evacuation insurance under this Grant
is purchased it shall be purchased from the insurance company
agent with which AID has a contract to provide DBAor insurance for AID contracts The Grant Officer will provide
the name address and telephone number of such insurance
company or agent upon request
IG INDIRECT COST RATE
IGI Pursuant to the Standard Provision of this Grant
entitled Negotiated Indirect Cost Rates - Provisional
(Nonprofits) and if applicable Negotiated Indirect Cost or rates shall beRates - Predetermined an indirect cost rate
the Grantees accounting periods whichestablished for each of this Grant Payment on account of allowable indirectapply to
on the basis of final or predeterminedcosts shall be made indirect cost rates for each accounting period which applies to
this Grant but not in excess of the following ceiling rate(s) applied to the base(s) which is (are) set forth below Any indirect costs above the ceiling rate(s) shall be absorbed by the Grantee and considered cost sharing
Rate Rase
On-CampusHome Office 520 1 Off-CampusOff-Site 291 1
J Base of Application direct salaries including vacation holiday sick pay and other paid absences but excluding all other fringe benefits
1H PROPERTY REQUIREMENTS
1HI Title to Property
Title to property acquired hereunder shall vest in the Grantee subject to the requirements of the Standard Provision of this grant entitled Title To and Use of Property (Grantee Title)
regarding use accountability and disposition of such property
(7
PCE-5053-G-00-3045-00
Page 10
1H2 Ecuipment Purchases
Equipment purchases under this Grant must be made in accordance
with the Standard Provisions entitled Procurement of Goods and
Services and AID Eligibility Rules for Goods and Services
included in Attachment 3 of this Grant Inclusion of costs in
the budget of this Grant for the purchase of nonexpendable equipment does not obviate the requirements of Section J13 of
OMB Circular A-21 (for educational institutions) or Section 13
of Attachment B of OMB Circular A-122 (for nonprofit organizations other than educational institutions) for prior
approval of such purchases by the Grant Officer nor any other
terms and conditions of this Grant unless specifically stated
in Section lH2(a)(3) below
IH2(a) Reguirement for Prior Approval
Pursuant to Sections 1D3 and 1G3 above and the Standard
Provisions of this Grant entitled Allowable Costs and
Revision of Grant Budget and by extension Section 13 of
Attachment B of OMB Circular A-122 the Grantee must obtain AID Grant Officer approval for purchases of the following
1H2(a)(1) General Purpose EQuipment which is defined as an
article of nonexpendable tangible personal property which is
usable for other than research medical scientific or technical
activities whether or not special modifications are needed to
make them suitable for a particular purpose (eg office
equipment and furnishings air conditioning equipment reproduction and printing equipment motor vehicles and
automatic data processing equipment) having a useful life of
more than two years and an acquisition cost of $500 or more per unit) and
IH2(a)(2) Special Purpose EguiPment which is defined as an
article of nonexpendable tangible personal property which is used only for research medical scientific or technical activities (eg microscopes x-ray machines surgical instruments and spectrometers) and which has a useful life of
more than two years and an acquisition cost of $1000 or more per unit)
1H2(a)(3) Approvals
In furtherance of the foregoing the Grant Officer does hereby provide approval for the following purchases which shall not be
construed as authorization to exceed the total estimated amount
or the obligated amount of this Grant whichever is less (see
Section 1C above)
- one microscope - two laboratory refrigerators
6)
PCE-5053-G-00-3045-00 Page 11
- two laboratoryfreezers - one diesel engine
one water pump and associated electrical materials-
IH2(a)(4) EMueption for Automation Eguipment
for the purchase of automation equipment which mayAny approval be provided in Section IH2(a)(3) above or subsequently
is not valid if the total cost of provided by the Grant Officer
purchases of automation equipment (eg computers word
related services made hereunder processors etc) software or
will exceed $100000 The Grantee must under such the Grant Officer for the
circumstances obtain the approval of any automation equipment software or total planned system of
related services
11 RESOLUTION OF CONFLICTS
the Attachments of this Grant shall beConflicts between any of
resolved by applying the following descending order of
precedence
Attachment 1 - Schedule - Standard ProvisionsAttachment 3
Attachment 2 - Program Description
Agency For International Development Research Grant Program
for Historically Black Colleges and Universities
Proposal Title Development and Dissemination of True Potato Seed Technology in Uganda
Institution Fort Valley State College Fort Valley Georgia 31030
Principal Investigator Dr A S Bhagsari Professor
AGENCY FOR INTERNATIONAL DEVELOPMENT RESEARCH GRANT PROGRAM
FOR HISTORICAL BLACK COLLEGES AND UNIVERSITIES
SUBMITTED BY FORT VALLEY STATE COLLEGE (FVSC) Agricultural Research Station 1005 State College Drive Fort Valley Georgia 31030-3298
PROPOSAL TITLE DEVELOPMENT AND DISSEMINATION OF TRUE POTATO SEED TECHNOLOGY IN UGANDA
PRINCIPAL INVESTIGATOR Ajmer SBhagsari Ph D (USA) Professor
Agricultural Research Station Fort Valley State College Fort Valley Georgia USA Telephone No (912) 825-6825 Fax No (912) 825-6376
CO-INVESTIGATOR Adipala Ekwamu PhD (Uganda) Faculty of Agriculture and Forestry
Department of Crop Science Makerere University Kampala Uganda Africa
DATE OF SUBMISSION April 1993
Signature of Principal Investigator Sgnature an Name
Agricultural Research Station Representative of Proposing Insfitution
Statements on Proposal Submission
This project proposal in part or in whole has not been submitted to any other1 funding agency and is not closely related to research being done for other sponsors
2 This project if approved will provide for its own logistic support and will not pose
any load on the USAID mission
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
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6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
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11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
PCE-5053-G-00-3045-00 Page 4
ATTACHMENT 1
SCHEDULE
IA PURPOSE OF GRANT
The purpose of this Grant is to provide financial support for the program described in Attachment 2 of this Grant entitled Program Description
1B PERIOD OF GRANT
The effective date of this Grant is the date of the Cover Letter and the estimated completion date is December 31 1995 Funds obligated hereunder (see Section IC2 below) shall be used to reimburse the Grantee for allowable program expenditures incurred by the Grantee in pursuit of program objectives during such period Funds obligated hereunder are anticipated to be sufficient for completion by the Grantee of the program described in Attachment 2 of this Grant by the estimated completion date
IC AMOUNT OF GRANT AND PAYMENT
ICI The total estimated amount of this Grant for its full period as set forth in Section lB above is $100000
1C2 AID hereby obligates the amount of $100000 for the purposes of this Grant during the indicated period set forth in Section lB above thereby fulfilling AIDs funding requirements AID shall not be liable for reimbursing the Grantee for any costs in excess of the obligated amount except as specified in paragraph (f) of the Standard Provision of this Grant entitled Revision of Grant Budget
1C3 Payment shall be made to the Grantee in accordance with procedures set forth in the Standard Provision of this Grant entitled Payment - Periodic Advance as shown in Attachment 3
ID GRANT BUDGET
IDl The following is the Budget for the total estimated amount of this Grant (see Section ICl above) for its full period (see Section IB above) The Grantee may not exceed the total estimated amount or the obligated amount of this Grant whichever is less (see Sections ICI and 1C2 respectively above) Except as specified in the Standard Provision of this Grant entitled Revision of Grant Budget as shown in Attachment 3 the Grantee may adjust line item amounts as may be reasonably necessary for the attainment of program objectives
PCE-053-G-00-3045-00
Page 5
1D2 Bde Cost-Sharing
Cost Element AID N etal
Salaries $54200 $0 $54200
Fringe Travel
$4350 $18500
$0 $0
$4350 $18500
Non-Exp Equip $10500 ODC $8085 Overhead 64
$0 $0
$10500 $8085
365
Total $100000 $0 $100000
1D3 Inclusion of any cost in the budget of this Grant does
not obviate the requirement for prior approval by the Grant
Officer of cost items designated as requiring prior approval by
the applicable cost principles (see the Standard Provision of
this Grant set forth in Attachment 3 entitled Allowable Costs)
and other terms and conditions of this Grant unless
specifically stated in Section I below
1El Financial Reportina
IE1(a) Financial reporting requirements shall be in
accordance with the Standard Provision of this Grant entitled
Payment - Periodic Advance as shown in Attachment 3 If a
Standard Form 269 is required by the aforesaid Standard said form shall be usedProvision the Long Form of
iE1(b) All financial reports shall be submitted to
AID Office of Financial Management FAFMCMPDDCB Room
700 SA-2 Washington DC 20523-0209 In addition three
copies of all financial reports shall be submitted to the
AID Project Office specified in the Cover Letter of this
Grant concurrently with submission of the Quarterly Technical
Reports (See Section 1E2 below)
1El(c) The frequency of financial reporting and the due
dates of reports shall be as specified in the Standard in Section iEl(a) aboveProvision of this Grant referred to
1E2 Program Reporting
1E2(a) Annual Workplan
The Grantee shall submit an annual workplan for1E2(a)(1) this Grant which shall contain the following
1E2(a)()(A) An action-oriented workplan describing
planned activities for the next year delineated by calendar
PCE-5053-G-00-3045-00 Page 6
and objectives whichquarter and linked to the project goals bedescribes the individuals to be involved the activities to
conducted and where and when they will be conducted Planned
activities shall be grouped by subject category and then
related to project objectives
1E2(a)(1)(B) A projected budget utilizing the same as are set forth in the budget of this Grantbudget line items
for each calendar quarter corresponding to the workplan and
1E2(a)(1)(C) Publications reports workshops seminars
and other information dissemination activities planned by
calendar quarter
The Grantee may develop the annual workplan in1E2(a)(2) consultation with the AID Project Officer for this Grant
lE2(a)(3) Five (5) copies of the annual workplan shall be
submitted to the designated AID Project Officer for this
Grant and one copy submitted to the Grant Officer The annual
workplan shall be submitted by the Grantee not later than sixty
(60) days from the effective date of this Grant (see Section
lB above)
IE2(b) Ouarterly Reports
The Grantee shall submit five (5) copies of brief quarterly
program performance reports which coincide with the financial
reporting periods described in Section lEI above to the
AID Project Office specified in the Cover Letter of this
Grant In addition two copies shall be submitted to AID
POLCDIEDI Washington DC 20523-1802 These reports shall be
submitted within 30 days following the end of the reporting
period and shall briefly present the following information
1E2(b)(1) A comparison of actual accomplishments with the
goals established for the period the findings of the investigator or both If the output of programs can be
readily quantified such quantitative data should be related to
cost data for computation of unit costs
of finances and expenditures and when appropriate analysis
1E2(b)(2) Reasons why established goals were not met if
applicable
1E2(b)(3) Other pertinent information including the status
and explanation of cost overruns or high unit costs
IE2(c) Special Reports
1E2(c)(1) Within 30 days following the completion of each a tripinternational trip the Grantee shall submit 3 copies of
PCE-5053-G-00-3045-00 Page 7
report summarizing the accomplishments of the trip to the
AID Project Officer specified in the cover letter of this
Grant If several individuals are travelling together to one The
site a single report representing the group will suffice
report shall include the purpose of the trip technical
observations suggestions and recommendations overall
impressions of the site situation (if appropriate) and a list
of persons visited with their title and organization
affiliation
Between the required program performance1E2(c)(2) that have significant impact
reporting dates events may occur inform upon the program In such instances the Grantee shall
the AID Project Officer as soon as the following types of
conditions become known
adverse conditions thatIE2(c)(2)(A) Problems delays or
attain programwill materially affect the ability to
objectives prevent the meeting of time schedules and goals or
preclude the attainment of work units by established time
This disclosure shall be accompanied by a statementperiods of the action taken or contemplated and any AID assistance
needed to resolve the situation
Favorable developments or events that enableIE2(c)(2)(B) sooner than anticipated or more worktime schedules to be met
units to be produced than originally projected
lE2(c)(3) If any performance review conducted by the
Grantee discloses the need for change in the budget estimates
in accordance with the criteria established in the Standard
this Grant entitled Revision of Grant BudgetProvision of the Grantee shall submit a request for budget revision to
the
Grant Officer and the AID Project Officer specified in the
Cover Letter of this Grant
IE2(d) Environmental Impact
this project will result inIf it appears that the outputs of
an adverse environmental impact the Grantee shall notify the
AID Project Officer prior to implementation in order to
allow for orderly preparation of an environmental impact that appropriate USstatement The Grantee shall assure
followedGovernment andor host country procedures are
1E2(e) Care of Laboratory Animals
If the Standard Provision entitled Care of Laboratory Animals
(see Section for applicability) theapplies to this Grant Grantee shall include the certificate required by paragraph
(c)
of said Standard Provision in all of its reports which pertain
to the use of laboratory animals
PCE-5053-G-00-3045-00
Page 8
IE2(f) Final Report
Within 90 days following the estimated completion date of this Grant (see Section lB above) the Grantee shall submit five (5) copies of a final report to the AID Project Office specified in the cover letter of this Grant In addition two copies shall be submitted to AID POLCDIEDI Washington DC 20523-1802 It will cover the entire period of the Grant and include all information shown in Sections 1E2(a) and 1E2(c) above
IF SPECIAL PROVISIONS
1F1 OPTIONAL STANDARD PROVISIONS
The following Optional Standard Provisions for US Nongovernmental Grantees as listed in Attachment 3 of this Grant are hereby deleted as follows
Provision Payment - Periodic Advance 13 Payment - Cost Reimbursement 15 Local Cost Financing 33 Patent Rights 35 Negotiated Indirect C-t Rates - Provisional 46 Participant Training 48 Voluntary Population Planning 49 Protection of the Individual as a Research Subject 56 Title To and Care of Property(US Government Title) 63 Title To and Care of Property(Cooperating Country Title)67 Cost Sharing 71
All other Optional Standard Provisions are hereby incorporated into this Grant and have been checked off on page 9 of Attachment 3 IF2 Limitations on Reimbursement of Costs of Compensation
for Personal Services and Professional Service Costs
IF2(a) Employee Salaries
Except as the Grant Officer may otherwise agree in writing AID shall not be liable for reimbursing the Grantee for any costs allocable to the salary portion of direct compensation paid by the Grantee to its employees for personal services which exceed the highest salary level for a Foreign Service Officer Class 1 (FS-l) as periodically amended
IF2(b) Consultant Fees
Compensation for consultants retained by the Grantee hereunder shall not exceed without specific approval of the rate by the Grant Officer either the highest rate of annual compensation
P2E-5053-G-00-3045-00 Page 9
received by the consultant during any full year of the
or the maximum rate of aimmediately preceding three years
Foreign Service Officer Class 1 (FS-l) (as periodically A daily rate is derived byamended) whichever is less
dividing the annual compensation by 2087 and multiplying the
result by 8
1F3 Defense Base Act (DBA) and Medical Evacuation Insurance
Section J16 of OMB Circular A-21 (for educationalPursuant to Section 18 of Attachment B of OMB Circularinstitutions) or
A-122 (for nonprofit organizations other than educational
institutions) the Grantee is authorized to purchase DBA andor If DBA insurancemedical evacuation insurance under this Grant
is purchased it shall be purchased from the insurance company
agent with which AID has a contract to provide DBAor insurance for AID contracts The Grant Officer will provide
the name address and telephone number of such insurance
company or agent upon request
IG INDIRECT COST RATE
IGI Pursuant to the Standard Provision of this Grant
entitled Negotiated Indirect Cost Rates - Provisional
(Nonprofits) and if applicable Negotiated Indirect Cost or rates shall beRates - Predetermined an indirect cost rate
the Grantees accounting periods whichestablished for each of this Grant Payment on account of allowable indirectapply to
on the basis of final or predeterminedcosts shall be made indirect cost rates for each accounting period which applies to
this Grant but not in excess of the following ceiling rate(s) applied to the base(s) which is (are) set forth below Any indirect costs above the ceiling rate(s) shall be absorbed by the Grantee and considered cost sharing
Rate Rase
On-CampusHome Office 520 1 Off-CampusOff-Site 291 1
J Base of Application direct salaries including vacation holiday sick pay and other paid absences but excluding all other fringe benefits
1H PROPERTY REQUIREMENTS
1HI Title to Property
Title to property acquired hereunder shall vest in the Grantee subject to the requirements of the Standard Provision of this grant entitled Title To and Use of Property (Grantee Title)
regarding use accountability and disposition of such property
(7
PCE-5053-G-00-3045-00
Page 10
1H2 Ecuipment Purchases
Equipment purchases under this Grant must be made in accordance
with the Standard Provisions entitled Procurement of Goods and
Services and AID Eligibility Rules for Goods and Services
included in Attachment 3 of this Grant Inclusion of costs in
the budget of this Grant for the purchase of nonexpendable equipment does not obviate the requirements of Section J13 of
OMB Circular A-21 (for educational institutions) or Section 13
of Attachment B of OMB Circular A-122 (for nonprofit organizations other than educational institutions) for prior
approval of such purchases by the Grant Officer nor any other
terms and conditions of this Grant unless specifically stated
in Section lH2(a)(3) below
IH2(a) Reguirement for Prior Approval
Pursuant to Sections 1D3 and 1G3 above and the Standard
Provisions of this Grant entitled Allowable Costs and
Revision of Grant Budget and by extension Section 13 of
Attachment B of OMB Circular A-122 the Grantee must obtain AID Grant Officer approval for purchases of the following
1H2(a)(1) General Purpose EQuipment which is defined as an
article of nonexpendable tangible personal property which is
usable for other than research medical scientific or technical
activities whether or not special modifications are needed to
make them suitable for a particular purpose (eg office
equipment and furnishings air conditioning equipment reproduction and printing equipment motor vehicles and
automatic data processing equipment) having a useful life of
more than two years and an acquisition cost of $500 or more per unit) and
IH2(a)(2) Special Purpose EguiPment which is defined as an
article of nonexpendable tangible personal property which is used only for research medical scientific or technical activities (eg microscopes x-ray machines surgical instruments and spectrometers) and which has a useful life of
more than two years and an acquisition cost of $1000 or more per unit)
1H2(a)(3) Approvals
In furtherance of the foregoing the Grant Officer does hereby provide approval for the following purchases which shall not be
construed as authorization to exceed the total estimated amount
or the obligated amount of this Grant whichever is less (see
Section 1C above)
- one microscope - two laboratory refrigerators
6)
PCE-5053-G-00-3045-00 Page 11
- two laboratoryfreezers - one diesel engine
one water pump and associated electrical materials-
IH2(a)(4) EMueption for Automation Eguipment
for the purchase of automation equipment which mayAny approval be provided in Section IH2(a)(3) above or subsequently
is not valid if the total cost of provided by the Grant Officer
purchases of automation equipment (eg computers word
related services made hereunder processors etc) software or
will exceed $100000 The Grantee must under such the Grant Officer for the
circumstances obtain the approval of any automation equipment software or total planned system of
related services
11 RESOLUTION OF CONFLICTS
the Attachments of this Grant shall beConflicts between any of
resolved by applying the following descending order of
precedence
Attachment 1 - Schedule - Standard ProvisionsAttachment 3
Attachment 2 - Program Description
Agency For International Development Research Grant Program
for Historically Black Colleges and Universities
Proposal Title Development and Dissemination of True Potato Seed Technology in Uganda
Institution Fort Valley State College Fort Valley Georgia 31030
Principal Investigator Dr A S Bhagsari Professor
AGENCY FOR INTERNATIONAL DEVELOPMENT RESEARCH GRANT PROGRAM
FOR HISTORICAL BLACK COLLEGES AND UNIVERSITIES
SUBMITTED BY FORT VALLEY STATE COLLEGE (FVSC) Agricultural Research Station 1005 State College Drive Fort Valley Georgia 31030-3298
PROPOSAL TITLE DEVELOPMENT AND DISSEMINATION OF TRUE POTATO SEED TECHNOLOGY IN UGANDA
PRINCIPAL INVESTIGATOR Ajmer SBhagsari Ph D (USA) Professor
Agricultural Research Station Fort Valley State College Fort Valley Georgia USA Telephone No (912) 825-6825 Fax No (912) 825-6376
CO-INVESTIGATOR Adipala Ekwamu PhD (Uganda) Faculty of Agriculture and Forestry
Department of Crop Science Makerere University Kampala Uganda Africa
DATE OF SUBMISSION April 1993
Signature of Principal Investigator Sgnature an Name
Agricultural Research Station Representative of Proposing Insfitution
Statements on Proposal Submission
This project proposal in part or in whole has not been submitted to any other1 funding agency and is not closely related to research being done for other sponsors
2 This project if approved will provide for its own logistic support and will not pose
any load on the USAID mission
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
PCE-053-G-00-3045-00
Page 5
1D2 Bde Cost-Sharing
Cost Element AID N etal
Salaries $54200 $0 $54200
Fringe Travel
$4350 $18500
$0 $0
$4350 $18500
Non-Exp Equip $10500 ODC $8085 Overhead 64
$0 $0
$10500 $8085
365
Total $100000 $0 $100000
1D3 Inclusion of any cost in the budget of this Grant does
not obviate the requirement for prior approval by the Grant
Officer of cost items designated as requiring prior approval by
the applicable cost principles (see the Standard Provision of
this Grant set forth in Attachment 3 entitled Allowable Costs)
and other terms and conditions of this Grant unless
specifically stated in Section I below
1El Financial Reportina
IE1(a) Financial reporting requirements shall be in
accordance with the Standard Provision of this Grant entitled
Payment - Periodic Advance as shown in Attachment 3 If a
Standard Form 269 is required by the aforesaid Standard said form shall be usedProvision the Long Form of
iE1(b) All financial reports shall be submitted to
AID Office of Financial Management FAFMCMPDDCB Room
700 SA-2 Washington DC 20523-0209 In addition three
copies of all financial reports shall be submitted to the
AID Project Office specified in the Cover Letter of this
Grant concurrently with submission of the Quarterly Technical
Reports (See Section 1E2 below)
1El(c) The frequency of financial reporting and the due
dates of reports shall be as specified in the Standard in Section iEl(a) aboveProvision of this Grant referred to
1E2 Program Reporting
1E2(a) Annual Workplan
The Grantee shall submit an annual workplan for1E2(a)(1) this Grant which shall contain the following
1E2(a)()(A) An action-oriented workplan describing
planned activities for the next year delineated by calendar
PCE-5053-G-00-3045-00 Page 6
and objectives whichquarter and linked to the project goals bedescribes the individuals to be involved the activities to
conducted and where and when they will be conducted Planned
activities shall be grouped by subject category and then
related to project objectives
1E2(a)(1)(B) A projected budget utilizing the same as are set forth in the budget of this Grantbudget line items
for each calendar quarter corresponding to the workplan and
1E2(a)(1)(C) Publications reports workshops seminars
and other information dissemination activities planned by
calendar quarter
The Grantee may develop the annual workplan in1E2(a)(2) consultation with the AID Project Officer for this Grant
lE2(a)(3) Five (5) copies of the annual workplan shall be
submitted to the designated AID Project Officer for this
Grant and one copy submitted to the Grant Officer The annual
workplan shall be submitted by the Grantee not later than sixty
(60) days from the effective date of this Grant (see Section
lB above)
IE2(b) Ouarterly Reports
The Grantee shall submit five (5) copies of brief quarterly
program performance reports which coincide with the financial
reporting periods described in Section lEI above to the
AID Project Office specified in the Cover Letter of this
Grant In addition two copies shall be submitted to AID
POLCDIEDI Washington DC 20523-1802 These reports shall be
submitted within 30 days following the end of the reporting
period and shall briefly present the following information
1E2(b)(1) A comparison of actual accomplishments with the
goals established for the period the findings of the investigator or both If the output of programs can be
readily quantified such quantitative data should be related to
cost data for computation of unit costs
of finances and expenditures and when appropriate analysis
1E2(b)(2) Reasons why established goals were not met if
applicable
1E2(b)(3) Other pertinent information including the status
and explanation of cost overruns or high unit costs
IE2(c) Special Reports
1E2(c)(1) Within 30 days following the completion of each a tripinternational trip the Grantee shall submit 3 copies of
PCE-5053-G-00-3045-00 Page 7
report summarizing the accomplishments of the trip to the
AID Project Officer specified in the cover letter of this
Grant If several individuals are travelling together to one The
site a single report representing the group will suffice
report shall include the purpose of the trip technical
observations suggestions and recommendations overall
impressions of the site situation (if appropriate) and a list
of persons visited with their title and organization
affiliation
Between the required program performance1E2(c)(2) that have significant impact
reporting dates events may occur inform upon the program In such instances the Grantee shall
the AID Project Officer as soon as the following types of
conditions become known
adverse conditions thatIE2(c)(2)(A) Problems delays or
attain programwill materially affect the ability to
objectives prevent the meeting of time schedules and goals or
preclude the attainment of work units by established time
This disclosure shall be accompanied by a statementperiods of the action taken or contemplated and any AID assistance
needed to resolve the situation
Favorable developments or events that enableIE2(c)(2)(B) sooner than anticipated or more worktime schedules to be met
units to be produced than originally projected
lE2(c)(3) If any performance review conducted by the
Grantee discloses the need for change in the budget estimates
in accordance with the criteria established in the Standard
this Grant entitled Revision of Grant BudgetProvision of the Grantee shall submit a request for budget revision to
the
Grant Officer and the AID Project Officer specified in the
Cover Letter of this Grant
IE2(d) Environmental Impact
this project will result inIf it appears that the outputs of
an adverse environmental impact the Grantee shall notify the
AID Project Officer prior to implementation in order to
allow for orderly preparation of an environmental impact that appropriate USstatement The Grantee shall assure
followedGovernment andor host country procedures are
1E2(e) Care of Laboratory Animals
If the Standard Provision entitled Care of Laboratory Animals
(see Section for applicability) theapplies to this Grant Grantee shall include the certificate required by paragraph
(c)
of said Standard Provision in all of its reports which pertain
to the use of laboratory animals
PCE-5053-G-00-3045-00
Page 8
IE2(f) Final Report
Within 90 days following the estimated completion date of this Grant (see Section lB above) the Grantee shall submit five (5) copies of a final report to the AID Project Office specified in the cover letter of this Grant In addition two copies shall be submitted to AID POLCDIEDI Washington DC 20523-1802 It will cover the entire period of the Grant and include all information shown in Sections 1E2(a) and 1E2(c) above
IF SPECIAL PROVISIONS
1F1 OPTIONAL STANDARD PROVISIONS
The following Optional Standard Provisions for US Nongovernmental Grantees as listed in Attachment 3 of this Grant are hereby deleted as follows
Provision Payment - Periodic Advance 13 Payment - Cost Reimbursement 15 Local Cost Financing 33 Patent Rights 35 Negotiated Indirect C-t Rates - Provisional 46 Participant Training 48 Voluntary Population Planning 49 Protection of the Individual as a Research Subject 56 Title To and Care of Property(US Government Title) 63 Title To and Care of Property(Cooperating Country Title)67 Cost Sharing 71
All other Optional Standard Provisions are hereby incorporated into this Grant and have been checked off on page 9 of Attachment 3 IF2 Limitations on Reimbursement of Costs of Compensation
for Personal Services and Professional Service Costs
IF2(a) Employee Salaries
Except as the Grant Officer may otherwise agree in writing AID shall not be liable for reimbursing the Grantee for any costs allocable to the salary portion of direct compensation paid by the Grantee to its employees for personal services which exceed the highest salary level for a Foreign Service Officer Class 1 (FS-l) as periodically amended
IF2(b) Consultant Fees
Compensation for consultants retained by the Grantee hereunder shall not exceed without specific approval of the rate by the Grant Officer either the highest rate of annual compensation
P2E-5053-G-00-3045-00 Page 9
received by the consultant during any full year of the
or the maximum rate of aimmediately preceding three years
Foreign Service Officer Class 1 (FS-l) (as periodically A daily rate is derived byamended) whichever is less
dividing the annual compensation by 2087 and multiplying the
result by 8
1F3 Defense Base Act (DBA) and Medical Evacuation Insurance
Section J16 of OMB Circular A-21 (for educationalPursuant to Section 18 of Attachment B of OMB Circularinstitutions) or
A-122 (for nonprofit organizations other than educational
institutions) the Grantee is authorized to purchase DBA andor If DBA insurancemedical evacuation insurance under this Grant
is purchased it shall be purchased from the insurance company
agent with which AID has a contract to provide DBAor insurance for AID contracts The Grant Officer will provide
the name address and telephone number of such insurance
company or agent upon request
IG INDIRECT COST RATE
IGI Pursuant to the Standard Provision of this Grant
entitled Negotiated Indirect Cost Rates - Provisional
(Nonprofits) and if applicable Negotiated Indirect Cost or rates shall beRates - Predetermined an indirect cost rate
the Grantees accounting periods whichestablished for each of this Grant Payment on account of allowable indirectapply to
on the basis of final or predeterminedcosts shall be made indirect cost rates for each accounting period which applies to
this Grant but not in excess of the following ceiling rate(s) applied to the base(s) which is (are) set forth below Any indirect costs above the ceiling rate(s) shall be absorbed by the Grantee and considered cost sharing
Rate Rase
On-CampusHome Office 520 1 Off-CampusOff-Site 291 1
J Base of Application direct salaries including vacation holiday sick pay and other paid absences but excluding all other fringe benefits
1H PROPERTY REQUIREMENTS
1HI Title to Property
Title to property acquired hereunder shall vest in the Grantee subject to the requirements of the Standard Provision of this grant entitled Title To and Use of Property (Grantee Title)
regarding use accountability and disposition of such property
(7
PCE-5053-G-00-3045-00
Page 10
1H2 Ecuipment Purchases
Equipment purchases under this Grant must be made in accordance
with the Standard Provisions entitled Procurement of Goods and
Services and AID Eligibility Rules for Goods and Services
included in Attachment 3 of this Grant Inclusion of costs in
the budget of this Grant for the purchase of nonexpendable equipment does not obviate the requirements of Section J13 of
OMB Circular A-21 (for educational institutions) or Section 13
of Attachment B of OMB Circular A-122 (for nonprofit organizations other than educational institutions) for prior
approval of such purchases by the Grant Officer nor any other
terms and conditions of this Grant unless specifically stated
in Section lH2(a)(3) below
IH2(a) Reguirement for Prior Approval
Pursuant to Sections 1D3 and 1G3 above and the Standard
Provisions of this Grant entitled Allowable Costs and
Revision of Grant Budget and by extension Section 13 of
Attachment B of OMB Circular A-122 the Grantee must obtain AID Grant Officer approval for purchases of the following
1H2(a)(1) General Purpose EQuipment which is defined as an
article of nonexpendable tangible personal property which is
usable for other than research medical scientific or technical
activities whether or not special modifications are needed to
make them suitable for a particular purpose (eg office
equipment and furnishings air conditioning equipment reproduction and printing equipment motor vehicles and
automatic data processing equipment) having a useful life of
more than two years and an acquisition cost of $500 or more per unit) and
IH2(a)(2) Special Purpose EguiPment which is defined as an
article of nonexpendable tangible personal property which is used only for research medical scientific or technical activities (eg microscopes x-ray machines surgical instruments and spectrometers) and which has a useful life of
more than two years and an acquisition cost of $1000 or more per unit)
1H2(a)(3) Approvals
In furtherance of the foregoing the Grant Officer does hereby provide approval for the following purchases which shall not be
construed as authorization to exceed the total estimated amount
or the obligated amount of this Grant whichever is less (see
Section 1C above)
- one microscope - two laboratory refrigerators
6)
PCE-5053-G-00-3045-00 Page 11
- two laboratoryfreezers - one diesel engine
one water pump and associated electrical materials-
IH2(a)(4) EMueption for Automation Eguipment
for the purchase of automation equipment which mayAny approval be provided in Section IH2(a)(3) above or subsequently
is not valid if the total cost of provided by the Grant Officer
purchases of automation equipment (eg computers word
related services made hereunder processors etc) software or
will exceed $100000 The Grantee must under such the Grant Officer for the
circumstances obtain the approval of any automation equipment software or total planned system of
related services
11 RESOLUTION OF CONFLICTS
the Attachments of this Grant shall beConflicts between any of
resolved by applying the following descending order of
precedence
Attachment 1 - Schedule - Standard ProvisionsAttachment 3
Attachment 2 - Program Description
Agency For International Development Research Grant Program
for Historically Black Colleges and Universities
Proposal Title Development and Dissemination of True Potato Seed Technology in Uganda
Institution Fort Valley State College Fort Valley Georgia 31030
Principal Investigator Dr A S Bhagsari Professor
AGENCY FOR INTERNATIONAL DEVELOPMENT RESEARCH GRANT PROGRAM
FOR HISTORICAL BLACK COLLEGES AND UNIVERSITIES
SUBMITTED BY FORT VALLEY STATE COLLEGE (FVSC) Agricultural Research Station 1005 State College Drive Fort Valley Georgia 31030-3298
PROPOSAL TITLE DEVELOPMENT AND DISSEMINATION OF TRUE POTATO SEED TECHNOLOGY IN UGANDA
PRINCIPAL INVESTIGATOR Ajmer SBhagsari Ph D (USA) Professor
Agricultural Research Station Fort Valley State College Fort Valley Georgia USA Telephone No (912) 825-6825 Fax No (912) 825-6376
CO-INVESTIGATOR Adipala Ekwamu PhD (Uganda) Faculty of Agriculture and Forestry
Department of Crop Science Makerere University Kampala Uganda Africa
DATE OF SUBMISSION April 1993
Signature of Principal Investigator Sgnature an Name
Agricultural Research Station Representative of Proposing Insfitution
Statements on Proposal Submission
This project proposal in part or in whole has not been submitted to any other1 funding agency and is not closely related to research being done for other sponsors
2 This project if approved will provide for its own logistic support and will not pose
any load on the USAID mission
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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23
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49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
PCE-5053-G-00-3045-00 Page 6
and objectives whichquarter and linked to the project goals bedescribes the individuals to be involved the activities to
conducted and where and when they will be conducted Planned
activities shall be grouped by subject category and then
related to project objectives
1E2(a)(1)(B) A projected budget utilizing the same as are set forth in the budget of this Grantbudget line items
for each calendar quarter corresponding to the workplan and
1E2(a)(1)(C) Publications reports workshops seminars
and other information dissemination activities planned by
calendar quarter
The Grantee may develop the annual workplan in1E2(a)(2) consultation with the AID Project Officer for this Grant
lE2(a)(3) Five (5) copies of the annual workplan shall be
submitted to the designated AID Project Officer for this
Grant and one copy submitted to the Grant Officer The annual
workplan shall be submitted by the Grantee not later than sixty
(60) days from the effective date of this Grant (see Section
lB above)
IE2(b) Ouarterly Reports
The Grantee shall submit five (5) copies of brief quarterly
program performance reports which coincide with the financial
reporting periods described in Section lEI above to the
AID Project Office specified in the Cover Letter of this
Grant In addition two copies shall be submitted to AID
POLCDIEDI Washington DC 20523-1802 These reports shall be
submitted within 30 days following the end of the reporting
period and shall briefly present the following information
1E2(b)(1) A comparison of actual accomplishments with the
goals established for the period the findings of the investigator or both If the output of programs can be
readily quantified such quantitative data should be related to
cost data for computation of unit costs
of finances and expenditures and when appropriate analysis
1E2(b)(2) Reasons why established goals were not met if
applicable
1E2(b)(3) Other pertinent information including the status
and explanation of cost overruns or high unit costs
IE2(c) Special Reports
1E2(c)(1) Within 30 days following the completion of each a tripinternational trip the Grantee shall submit 3 copies of
PCE-5053-G-00-3045-00 Page 7
report summarizing the accomplishments of the trip to the
AID Project Officer specified in the cover letter of this
Grant If several individuals are travelling together to one The
site a single report representing the group will suffice
report shall include the purpose of the trip technical
observations suggestions and recommendations overall
impressions of the site situation (if appropriate) and a list
of persons visited with their title and organization
affiliation
Between the required program performance1E2(c)(2) that have significant impact
reporting dates events may occur inform upon the program In such instances the Grantee shall
the AID Project Officer as soon as the following types of
conditions become known
adverse conditions thatIE2(c)(2)(A) Problems delays or
attain programwill materially affect the ability to
objectives prevent the meeting of time schedules and goals or
preclude the attainment of work units by established time
This disclosure shall be accompanied by a statementperiods of the action taken or contemplated and any AID assistance
needed to resolve the situation
Favorable developments or events that enableIE2(c)(2)(B) sooner than anticipated or more worktime schedules to be met
units to be produced than originally projected
lE2(c)(3) If any performance review conducted by the
Grantee discloses the need for change in the budget estimates
in accordance with the criteria established in the Standard
this Grant entitled Revision of Grant BudgetProvision of the Grantee shall submit a request for budget revision to
the
Grant Officer and the AID Project Officer specified in the
Cover Letter of this Grant
IE2(d) Environmental Impact
this project will result inIf it appears that the outputs of
an adverse environmental impact the Grantee shall notify the
AID Project Officer prior to implementation in order to
allow for orderly preparation of an environmental impact that appropriate USstatement The Grantee shall assure
followedGovernment andor host country procedures are
1E2(e) Care of Laboratory Animals
If the Standard Provision entitled Care of Laboratory Animals
(see Section for applicability) theapplies to this Grant Grantee shall include the certificate required by paragraph
(c)
of said Standard Provision in all of its reports which pertain
to the use of laboratory animals
PCE-5053-G-00-3045-00
Page 8
IE2(f) Final Report
Within 90 days following the estimated completion date of this Grant (see Section lB above) the Grantee shall submit five (5) copies of a final report to the AID Project Office specified in the cover letter of this Grant In addition two copies shall be submitted to AID POLCDIEDI Washington DC 20523-1802 It will cover the entire period of the Grant and include all information shown in Sections 1E2(a) and 1E2(c) above
IF SPECIAL PROVISIONS
1F1 OPTIONAL STANDARD PROVISIONS
The following Optional Standard Provisions for US Nongovernmental Grantees as listed in Attachment 3 of this Grant are hereby deleted as follows
Provision Payment - Periodic Advance 13 Payment - Cost Reimbursement 15 Local Cost Financing 33 Patent Rights 35 Negotiated Indirect C-t Rates - Provisional 46 Participant Training 48 Voluntary Population Planning 49 Protection of the Individual as a Research Subject 56 Title To and Care of Property(US Government Title) 63 Title To and Care of Property(Cooperating Country Title)67 Cost Sharing 71
All other Optional Standard Provisions are hereby incorporated into this Grant and have been checked off on page 9 of Attachment 3 IF2 Limitations on Reimbursement of Costs of Compensation
for Personal Services and Professional Service Costs
IF2(a) Employee Salaries
Except as the Grant Officer may otherwise agree in writing AID shall not be liable for reimbursing the Grantee for any costs allocable to the salary portion of direct compensation paid by the Grantee to its employees for personal services which exceed the highest salary level for a Foreign Service Officer Class 1 (FS-l) as periodically amended
IF2(b) Consultant Fees
Compensation for consultants retained by the Grantee hereunder shall not exceed without specific approval of the rate by the Grant Officer either the highest rate of annual compensation
P2E-5053-G-00-3045-00 Page 9
received by the consultant during any full year of the
or the maximum rate of aimmediately preceding three years
Foreign Service Officer Class 1 (FS-l) (as periodically A daily rate is derived byamended) whichever is less
dividing the annual compensation by 2087 and multiplying the
result by 8
1F3 Defense Base Act (DBA) and Medical Evacuation Insurance
Section J16 of OMB Circular A-21 (for educationalPursuant to Section 18 of Attachment B of OMB Circularinstitutions) or
A-122 (for nonprofit organizations other than educational
institutions) the Grantee is authorized to purchase DBA andor If DBA insurancemedical evacuation insurance under this Grant
is purchased it shall be purchased from the insurance company
agent with which AID has a contract to provide DBAor insurance for AID contracts The Grant Officer will provide
the name address and telephone number of such insurance
company or agent upon request
IG INDIRECT COST RATE
IGI Pursuant to the Standard Provision of this Grant
entitled Negotiated Indirect Cost Rates - Provisional
(Nonprofits) and if applicable Negotiated Indirect Cost or rates shall beRates - Predetermined an indirect cost rate
the Grantees accounting periods whichestablished for each of this Grant Payment on account of allowable indirectapply to
on the basis of final or predeterminedcosts shall be made indirect cost rates for each accounting period which applies to
this Grant but not in excess of the following ceiling rate(s) applied to the base(s) which is (are) set forth below Any indirect costs above the ceiling rate(s) shall be absorbed by the Grantee and considered cost sharing
Rate Rase
On-CampusHome Office 520 1 Off-CampusOff-Site 291 1
J Base of Application direct salaries including vacation holiday sick pay and other paid absences but excluding all other fringe benefits
1H PROPERTY REQUIREMENTS
1HI Title to Property
Title to property acquired hereunder shall vest in the Grantee subject to the requirements of the Standard Provision of this grant entitled Title To and Use of Property (Grantee Title)
regarding use accountability and disposition of such property
(7
PCE-5053-G-00-3045-00
Page 10
1H2 Ecuipment Purchases
Equipment purchases under this Grant must be made in accordance
with the Standard Provisions entitled Procurement of Goods and
Services and AID Eligibility Rules for Goods and Services
included in Attachment 3 of this Grant Inclusion of costs in
the budget of this Grant for the purchase of nonexpendable equipment does not obviate the requirements of Section J13 of
OMB Circular A-21 (for educational institutions) or Section 13
of Attachment B of OMB Circular A-122 (for nonprofit organizations other than educational institutions) for prior
approval of such purchases by the Grant Officer nor any other
terms and conditions of this Grant unless specifically stated
in Section lH2(a)(3) below
IH2(a) Reguirement for Prior Approval
Pursuant to Sections 1D3 and 1G3 above and the Standard
Provisions of this Grant entitled Allowable Costs and
Revision of Grant Budget and by extension Section 13 of
Attachment B of OMB Circular A-122 the Grantee must obtain AID Grant Officer approval for purchases of the following
1H2(a)(1) General Purpose EQuipment which is defined as an
article of nonexpendable tangible personal property which is
usable for other than research medical scientific or technical
activities whether or not special modifications are needed to
make them suitable for a particular purpose (eg office
equipment and furnishings air conditioning equipment reproduction and printing equipment motor vehicles and
automatic data processing equipment) having a useful life of
more than two years and an acquisition cost of $500 or more per unit) and
IH2(a)(2) Special Purpose EguiPment which is defined as an
article of nonexpendable tangible personal property which is used only for research medical scientific or technical activities (eg microscopes x-ray machines surgical instruments and spectrometers) and which has a useful life of
more than two years and an acquisition cost of $1000 or more per unit)
1H2(a)(3) Approvals
In furtherance of the foregoing the Grant Officer does hereby provide approval for the following purchases which shall not be
construed as authorization to exceed the total estimated amount
or the obligated amount of this Grant whichever is less (see
Section 1C above)
- one microscope - two laboratory refrigerators
6)
PCE-5053-G-00-3045-00 Page 11
- two laboratoryfreezers - one diesel engine
one water pump and associated electrical materials-
IH2(a)(4) EMueption for Automation Eguipment
for the purchase of automation equipment which mayAny approval be provided in Section IH2(a)(3) above or subsequently
is not valid if the total cost of provided by the Grant Officer
purchases of automation equipment (eg computers word
related services made hereunder processors etc) software or
will exceed $100000 The Grantee must under such the Grant Officer for the
circumstances obtain the approval of any automation equipment software or total planned system of
related services
11 RESOLUTION OF CONFLICTS
the Attachments of this Grant shall beConflicts between any of
resolved by applying the following descending order of
precedence
Attachment 1 - Schedule - Standard ProvisionsAttachment 3
Attachment 2 - Program Description
Agency For International Development Research Grant Program
for Historically Black Colleges and Universities
Proposal Title Development and Dissemination of True Potato Seed Technology in Uganda
Institution Fort Valley State College Fort Valley Georgia 31030
Principal Investigator Dr A S Bhagsari Professor
AGENCY FOR INTERNATIONAL DEVELOPMENT RESEARCH GRANT PROGRAM
FOR HISTORICAL BLACK COLLEGES AND UNIVERSITIES
SUBMITTED BY FORT VALLEY STATE COLLEGE (FVSC) Agricultural Research Station 1005 State College Drive Fort Valley Georgia 31030-3298
PROPOSAL TITLE DEVELOPMENT AND DISSEMINATION OF TRUE POTATO SEED TECHNOLOGY IN UGANDA
PRINCIPAL INVESTIGATOR Ajmer SBhagsari Ph D (USA) Professor
Agricultural Research Station Fort Valley State College Fort Valley Georgia USA Telephone No (912) 825-6825 Fax No (912) 825-6376
CO-INVESTIGATOR Adipala Ekwamu PhD (Uganda) Faculty of Agriculture and Forestry
Department of Crop Science Makerere University Kampala Uganda Africa
DATE OF SUBMISSION April 1993
Signature of Principal Investigator Sgnature an Name
Agricultural Research Station Representative of Proposing Insfitution
Statements on Proposal Submission
This project proposal in part or in whole has not been submitted to any other1 funding agency and is not closely related to research being done for other sponsors
2 This project if approved will provide for its own logistic support and will not pose
any load on the USAID mission
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
PCE-5053-G-00-3045-00 Page 7
report summarizing the accomplishments of the trip to the
AID Project Officer specified in the cover letter of this
Grant If several individuals are travelling together to one The
site a single report representing the group will suffice
report shall include the purpose of the trip technical
observations suggestions and recommendations overall
impressions of the site situation (if appropriate) and a list
of persons visited with their title and organization
affiliation
Between the required program performance1E2(c)(2) that have significant impact
reporting dates events may occur inform upon the program In such instances the Grantee shall
the AID Project Officer as soon as the following types of
conditions become known
adverse conditions thatIE2(c)(2)(A) Problems delays or
attain programwill materially affect the ability to
objectives prevent the meeting of time schedules and goals or
preclude the attainment of work units by established time
This disclosure shall be accompanied by a statementperiods of the action taken or contemplated and any AID assistance
needed to resolve the situation
Favorable developments or events that enableIE2(c)(2)(B) sooner than anticipated or more worktime schedules to be met
units to be produced than originally projected
lE2(c)(3) If any performance review conducted by the
Grantee discloses the need for change in the budget estimates
in accordance with the criteria established in the Standard
this Grant entitled Revision of Grant BudgetProvision of the Grantee shall submit a request for budget revision to
the
Grant Officer and the AID Project Officer specified in the
Cover Letter of this Grant
IE2(d) Environmental Impact
this project will result inIf it appears that the outputs of
an adverse environmental impact the Grantee shall notify the
AID Project Officer prior to implementation in order to
allow for orderly preparation of an environmental impact that appropriate USstatement The Grantee shall assure
followedGovernment andor host country procedures are
1E2(e) Care of Laboratory Animals
If the Standard Provision entitled Care of Laboratory Animals
(see Section for applicability) theapplies to this Grant Grantee shall include the certificate required by paragraph
(c)
of said Standard Provision in all of its reports which pertain
to the use of laboratory animals
PCE-5053-G-00-3045-00
Page 8
IE2(f) Final Report
Within 90 days following the estimated completion date of this Grant (see Section lB above) the Grantee shall submit five (5) copies of a final report to the AID Project Office specified in the cover letter of this Grant In addition two copies shall be submitted to AID POLCDIEDI Washington DC 20523-1802 It will cover the entire period of the Grant and include all information shown in Sections 1E2(a) and 1E2(c) above
IF SPECIAL PROVISIONS
1F1 OPTIONAL STANDARD PROVISIONS
The following Optional Standard Provisions for US Nongovernmental Grantees as listed in Attachment 3 of this Grant are hereby deleted as follows
Provision Payment - Periodic Advance 13 Payment - Cost Reimbursement 15 Local Cost Financing 33 Patent Rights 35 Negotiated Indirect C-t Rates - Provisional 46 Participant Training 48 Voluntary Population Planning 49 Protection of the Individual as a Research Subject 56 Title To and Care of Property(US Government Title) 63 Title To and Care of Property(Cooperating Country Title)67 Cost Sharing 71
All other Optional Standard Provisions are hereby incorporated into this Grant and have been checked off on page 9 of Attachment 3 IF2 Limitations on Reimbursement of Costs of Compensation
for Personal Services and Professional Service Costs
IF2(a) Employee Salaries
Except as the Grant Officer may otherwise agree in writing AID shall not be liable for reimbursing the Grantee for any costs allocable to the salary portion of direct compensation paid by the Grantee to its employees for personal services which exceed the highest salary level for a Foreign Service Officer Class 1 (FS-l) as periodically amended
IF2(b) Consultant Fees
Compensation for consultants retained by the Grantee hereunder shall not exceed without specific approval of the rate by the Grant Officer either the highest rate of annual compensation
P2E-5053-G-00-3045-00 Page 9
received by the consultant during any full year of the
or the maximum rate of aimmediately preceding three years
Foreign Service Officer Class 1 (FS-l) (as periodically A daily rate is derived byamended) whichever is less
dividing the annual compensation by 2087 and multiplying the
result by 8
1F3 Defense Base Act (DBA) and Medical Evacuation Insurance
Section J16 of OMB Circular A-21 (for educationalPursuant to Section 18 of Attachment B of OMB Circularinstitutions) or
A-122 (for nonprofit organizations other than educational
institutions) the Grantee is authorized to purchase DBA andor If DBA insurancemedical evacuation insurance under this Grant
is purchased it shall be purchased from the insurance company
agent with which AID has a contract to provide DBAor insurance for AID contracts The Grant Officer will provide
the name address and telephone number of such insurance
company or agent upon request
IG INDIRECT COST RATE
IGI Pursuant to the Standard Provision of this Grant
entitled Negotiated Indirect Cost Rates - Provisional
(Nonprofits) and if applicable Negotiated Indirect Cost or rates shall beRates - Predetermined an indirect cost rate
the Grantees accounting periods whichestablished for each of this Grant Payment on account of allowable indirectapply to
on the basis of final or predeterminedcosts shall be made indirect cost rates for each accounting period which applies to
this Grant but not in excess of the following ceiling rate(s) applied to the base(s) which is (are) set forth below Any indirect costs above the ceiling rate(s) shall be absorbed by the Grantee and considered cost sharing
Rate Rase
On-CampusHome Office 520 1 Off-CampusOff-Site 291 1
J Base of Application direct salaries including vacation holiday sick pay and other paid absences but excluding all other fringe benefits
1H PROPERTY REQUIREMENTS
1HI Title to Property
Title to property acquired hereunder shall vest in the Grantee subject to the requirements of the Standard Provision of this grant entitled Title To and Use of Property (Grantee Title)
regarding use accountability and disposition of such property
(7
PCE-5053-G-00-3045-00
Page 10
1H2 Ecuipment Purchases
Equipment purchases under this Grant must be made in accordance
with the Standard Provisions entitled Procurement of Goods and
Services and AID Eligibility Rules for Goods and Services
included in Attachment 3 of this Grant Inclusion of costs in
the budget of this Grant for the purchase of nonexpendable equipment does not obviate the requirements of Section J13 of
OMB Circular A-21 (for educational institutions) or Section 13
of Attachment B of OMB Circular A-122 (for nonprofit organizations other than educational institutions) for prior
approval of such purchases by the Grant Officer nor any other
terms and conditions of this Grant unless specifically stated
in Section lH2(a)(3) below
IH2(a) Reguirement for Prior Approval
Pursuant to Sections 1D3 and 1G3 above and the Standard
Provisions of this Grant entitled Allowable Costs and
Revision of Grant Budget and by extension Section 13 of
Attachment B of OMB Circular A-122 the Grantee must obtain AID Grant Officer approval for purchases of the following
1H2(a)(1) General Purpose EQuipment which is defined as an
article of nonexpendable tangible personal property which is
usable for other than research medical scientific or technical
activities whether or not special modifications are needed to
make them suitable for a particular purpose (eg office
equipment and furnishings air conditioning equipment reproduction and printing equipment motor vehicles and
automatic data processing equipment) having a useful life of
more than two years and an acquisition cost of $500 or more per unit) and
IH2(a)(2) Special Purpose EguiPment which is defined as an
article of nonexpendable tangible personal property which is used only for research medical scientific or technical activities (eg microscopes x-ray machines surgical instruments and spectrometers) and which has a useful life of
more than two years and an acquisition cost of $1000 or more per unit)
1H2(a)(3) Approvals
In furtherance of the foregoing the Grant Officer does hereby provide approval for the following purchases which shall not be
construed as authorization to exceed the total estimated amount
or the obligated amount of this Grant whichever is less (see
Section 1C above)
- one microscope - two laboratory refrigerators
6)
PCE-5053-G-00-3045-00 Page 11
- two laboratoryfreezers - one diesel engine
one water pump and associated electrical materials-
IH2(a)(4) EMueption for Automation Eguipment
for the purchase of automation equipment which mayAny approval be provided in Section IH2(a)(3) above or subsequently
is not valid if the total cost of provided by the Grant Officer
purchases of automation equipment (eg computers word
related services made hereunder processors etc) software or
will exceed $100000 The Grantee must under such the Grant Officer for the
circumstances obtain the approval of any automation equipment software or total planned system of
related services
11 RESOLUTION OF CONFLICTS
the Attachments of this Grant shall beConflicts between any of
resolved by applying the following descending order of
precedence
Attachment 1 - Schedule - Standard ProvisionsAttachment 3
Attachment 2 - Program Description
Agency For International Development Research Grant Program
for Historically Black Colleges and Universities
Proposal Title Development and Dissemination of True Potato Seed Technology in Uganda
Institution Fort Valley State College Fort Valley Georgia 31030
Principal Investigator Dr A S Bhagsari Professor
AGENCY FOR INTERNATIONAL DEVELOPMENT RESEARCH GRANT PROGRAM
FOR HISTORICAL BLACK COLLEGES AND UNIVERSITIES
SUBMITTED BY FORT VALLEY STATE COLLEGE (FVSC) Agricultural Research Station 1005 State College Drive Fort Valley Georgia 31030-3298
PROPOSAL TITLE DEVELOPMENT AND DISSEMINATION OF TRUE POTATO SEED TECHNOLOGY IN UGANDA
PRINCIPAL INVESTIGATOR Ajmer SBhagsari Ph D (USA) Professor
Agricultural Research Station Fort Valley State College Fort Valley Georgia USA Telephone No (912) 825-6825 Fax No (912) 825-6376
CO-INVESTIGATOR Adipala Ekwamu PhD (Uganda) Faculty of Agriculture and Forestry
Department of Crop Science Makerere University Kampala Uganda Africa
DATE OF SUBMISSION April 1993
Signature of Principal Investigator Sgnature an Name
Agricultural Research Station Representative of Proposing Insfitution
Statements on Proposal Submission
This project proposal in part or in whole has not been submitted to any other1 funding agency and is not closely related to research being done for other sponsors
2 This project if approved will provide for its own logistic support and will not pose
any load on the USAID mission
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
PCE-5053-G-00-3045-00
Page 8
IE2(f) Final Report
Within 90 days following the estimated completion date of this Grant (see Section lB above) the Grantee shall submit five (5) copies of a final report to the AID Project Office specified in the cover letter of this Grant In addition two copies shall be submitted to AID POLCDIEDI Washington DC 20523-1802 It will cover the entire period of the Grant and include all information shown in Sections 1E2(a) and 1E2(c) above
IF SPECIAL PROVISIONS
1F1 OPTIONAL STANDARD PROVISIONS
The following Optional Standard Provisions for US Nongovernmental Grantees as listed in Attachment 3 of this Grant are hereby deleted as follows
Provision Payment - Periodic Advance 13 Payment - Cost Reimbursement 15 Local Cost Financing 33 Patent Rights 35 Negotiated Indirect C-t Rates - Provisional 46 Participant Training 48 Voluntary Population Planning 49 Protection of the Individual as a Research Subject 56 Title To and Care of Property(US Government Title) 63 Title To and Care of Property(Cooperating Country Title)67 Cost Sharing 71
All other Optional Standard Provisions are hereby incorporated into this Grant and have been checked off on page 9 of Attachment 3 IF2 Limitations on Reimbursement of Costs of Compensation
for Personal Services and Professional Service Costs
IF2(a) Employee Salaries
Except as the Grant Officer may otherwise agree in writing AID shall not be liable for reimbursing the Grantee for any costs allocable to the salary portion of direct compensation paid by the Grantee to its employees for personal services which exceed the highest salary level for a Foreign Service Officer Class 1 (FS-l) as periodically amended
IF2(b) Consultant Fees
Compensation for consultants retained by the Grantee hereunder shall not exceed without specific approval of the rate by the Grant Officer either the highest rate of annual compensation
P2E-5053-G-00-3045-00 Page 9
received by the consultant during any full year of the
or the maximum rate of aimmediately preceding three years
Foreign Service Officer Class 1 (FS-l) (as periodically A daily rate is derived byamended) whichever is less
dividing the annual compensation by 2087 and multiplying the
result by 8
1F3 Defense Base Act (DBA) and Medical Evacuation Insurance
Section J16 of OMB Circular A-21 (for educationalPursuant to Section 18 of Attachment B of OMB Circularinstitutions) or
A-122 (for nonprofit organizations other than educational
institutions) the Grantee is authorized to purchase DBA andor If DBA insurancemedical evacuation insurance under this Grant
is purchased it shall be purchased from the insurance company
agent with which AID has a contract to provide DBAor insurance for AID contracts The Grant Officer will provide
the name address and telephone number of such insurance
company or agent upon request
IG INDIRECT COST RATE
IGI Pursuant to the Standard Provision of this Grant
entitled Negotiated Indirect Cost Rates - Provisional
(Nonprofits) and if applicable Negotiated Indirect Cost or rates shall beRates - Predetermined an indirect cost rate
the Grantees accounting periods whichestablished for each of this Grant Payment on account of allowable indirectapply to
on the basis of final or predeterminedcosts shall be made indirect cost rates for each accounting period which applies to
this Grant but not in excess of the following ceiling rate(s) applied to the base(s) which is (are) set forth below Any indirect costs above the ceiling rate(s) shall be absorbed by the Grantee and considered cost sharing
Rate Rase
On-CampusHome Office 520 1 Off-CampusOff-Site 291 1
J Base of Application direct salaries including vacation holiday sick pay and other paid absences but excluding all other fringe benefits
1H PROPERTY REQUIREMENTS
1HI Title to Property
Title to property acquired hereunder shall vest in the Grantee subject to the requirements of the Standard Provision of this grant entitled Title To and Use of Property (Grantee Title)
regarding use accountability and disposition of such property
(7
PCE-5053-G-00-3045-00
Page 10
1H2 Ecuipment Purchases
Equipment purchases under this Grant must be made in accordance
with the Standard Provisions entitled Procurement of Goods and
Services and AID Eligibility Rules for Goods and Services
included in Attachment 3 of this Grant Inclusion of costs in
the budget of this Grant for the purchase of nonexpendable equipment does not obviate the requirements of Section J13 of
OMB Circular A-21 (for educational institutions) or Section 13
of Attachment B of OMB Circular A-122 (for nonprofit organizations other than educational institutions) for prior
approval of such purchases by the Grant Officer nor any other
terms and conditions of this Grant unless specifically stated
in Section lH2(a)(3) below
IH2(a) Reguirement for Prior Approval
Pursuant to Sections 1D3 and 1G3 above and the Standard
Provisions of this Grant entitled Allowable Costs and
Revision of Grant Budget and by extension Section 13 of
Attachment B of OMB Circular A-122 the Grantee must obtain AID Grant Officer approval for purchases of the following
1H2(a)(1) General Purpose EQuipment which is defined as an
article of nonexpendable tangible personal property which is
usable for other than research medical scientific or technical
activities whether or not special modifications are needed to
make them suitable for a particular purpose (eg office
equipment and furnishings air conditioning equipment reproduction and printing equipment motor vehicles and
automatic data processing equipment) having a useful life of
more than two years and an acquisition cost of $500 or more per unit) and
IH2(a)(2) Special Purpose EguiPment which is defined as an
article of nonexpendable tangible personal property which is used only for research medical scientific or technical activities (eg microscopes x-ray machines surgical instruments and spectrometers) and which has a useful life of
more than two years and an acquisition cost of $1000 or more per unit)
1H2(a)(3) Approvals
In furtherance of the foregoing the Grant Officer does hereby provide approval for the following purchases which shall not be
construed as authorization to exceed the total estimated amount
or the obligated amount of this Grant whichever is less (see
Section 1C above)
- one microscope - two laboratory refrigerators
6)
PCE-5053-G-00-3045-00 Page 11
- two laboratoryfreezers - one diesel engine
one water pump and associated electrical materials-
IH2(a)(4) EMueption for Automation Eguipment
for the purchase of automation equipment which mayAny approval be provided in Section IH2(a)(3) above or subsequently
is not valid if the total cost of provided by the Grant Officer
purchases of automation equipment (eg computers word
related services made hereunder processors etc) software or
will exceed $100000 The Grantee must under such the Grant Officer for the
circumstances obtain the approval of any automation equipment software or total planned system of
related services
11 RESOLUTION OF CONFLICTS
the Attachments of this Grant shall beConflicts between any of
resolved by applying the following descending order of
precedence
Attachment 1 - Schedule - Standard ProvisionsAttachment 3
Attachment 2 - Program Description
Agency For International Development Research Grant Program
for Historically Black Colleges and Universities
Proposal Title Development and Dissemination of True Potato Seed Technology in Uganda
Institution Fort Valley State College Fort Valley Georgia 31030
Principal Investigator Dr A S Bhagsari Professor
AGENCY FOR INTERNATIONAL DEVELOPMENT RESEARCH GRANT PROGRAM
FOR HISTORICAL BLACK COLLEGES AND UNIVERSITIES
SUBMITTED BY FORT VALLEY STATE COLLEGE (FVSC) Agricultural Research Station 1005 State College Drive Fort Valley Georgia 31030-3298
PROPOSAL TITLE DEVELOPMENT AND DISSEMINATION OF TRUE POTATO SEED TECHNOLOGY IN UGANDA
PRINCIPAL INVESTIGATOR Ajmer SBhagsari Ph D (USA) Professor
Agricultural Research Station Fort Valley State College Fort Valley Georgia USA Telephone No (912) 825-6825 Fax No (912) 825-6376
CO-INVESTIGATOR Adipala Ekwamu PhD (Uganda) Faculty of Agriculture and Forestry
Department of Crop Science Makerere University Kampala Uganda Africa
DATE OF SUBMISSION April 1993
Signature of Principal Investigator Sgnature an Name
Agricultural Research Station Representative of Proposing Insfitution
Statements on Proposal Submission
This project proposal in part or in whole has not been submitted to any other1 funding agency and is not closely related to research being done for other sponsors
2 This project if approved will provide for its own logistic support and will not pose
any load on the USAID mission
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
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9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
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12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
P2E-5053-G-00-3045-00 Page 9
received by the consultant during any full year of the
or the maximum rate of aimmediately preceding three years
Foreign Service Officer Class 1 (FS-l) (as periodically A daily rate is derived byamended) whichever is less
dividing the annual compensation by 2087 and multiplying the
result by 8
1F3 Defense Base Act (DBA) and Medical Evacuation Insurance
Section J16 of OMB Circular A-21 (for educationalPursuant to Section 18 of Attachment B of OMB Circularinstitutions) or
A-122 (for nonprofit organizations other than educational
institutions) the Grantee is authorized to purchase DBA andor If DBA insurancemedical evacuation insurance under this Grant
is purchased it shall be purchased from the insurance company
agent with which AID has a contract to provide DBAor insurance for AID contracts The Grant Officer will provide
the name address and telephone number of such insurance
company or agent upon request
IG INDIRECT COST RATE
IGI Pursuant to the Standard Provision of this Grant
entitled Negotiated Indirect Cost Rates - Provisional
(Nonprofits) and if applicable Negotiated Indirect Cost or rates shall beRates - Predetermined an indirect cost rate
the Grantees accounting periods whichestablished for each of this Grant Payment on account of allowable indirectapply to
on the basis of final or predeterminedcosts shall be made indirect cost rates for each accounting period which applies to
this Grant but not in excess of the following ceiling rate(s) applied to the base(s) which is (are) set forth below Any indirect costs above the ceiling rate(s) shall be absorbed by the Grantee and considered cost sharing
Rate Rase
On-CampusHome Office 520 1 Off-CampusOff-Site 291 1
J Base of Application direct salaries including vacation holiday sick pay and other paid absences but excluding all other fringe benefits
1H PROPERTY REQUIREMENTS
1HI Title to Property
Title to property acquired hereunder shall vest in the Grantee subject to the requirements of the Standard Provision of this grant entitled Title To and Use of Property (Grantee Title)
regarding use accountability and disposition of such property
(7
PCE-5053-G-00-3045-00
Page 10
1H2 Ecuipment Purchases
Equipment purchases under this Grant must be made in accordance
with the Standard Provisions entitled Procurement of Goods and
Services and AID Eligibility Rules for Goods and Services
included in Attachment 3 of this Grant Inclusion of costs in
the budget of this Grant for the purchase of nonexpendable equipment does not obviate the requirements of Section J13 of
OMB Circular A-21 (for educational institutions) or Section 13
of Attachment B of OMB Circular A-122 (for nonprofit organizations other than educational institutions) for prior
approval of such purchases by the Grant Officer nor any other
terms and conditions of this Grant unless specifically stated
in Section lH2(a)(3) below
IH2(a) Reguirement for Prior Approval
Pursuant to Sections 1D3 and 1G3 above and the Standard
Provisions of this Grant entitled Allowable Costs and
Revision of Grant Budget and by extension Section 13 of
Attachment B of OMB Circular A-122 the Grantee must obtain AID Grant Officer approval for purchases of the following
1H2(a)(1) General Purpose EQuipment which is defined as an
article of nonexpendable tangible personal property which is
usable for other than research medical scientific or technical
activities whether or not special modifications are needed to
make them suitable for a particular purpose (eg office
equipment and furnishings air conditioning equipment reproduction and printing equipment motor vehicles and
automatic data processing equipment) having a useful life of
more than two years and an acquisition cost of $500 or more per unit) and
IH2(a)(2) Special Purpose EguiPment which is defined as an
article of nonexpendable tangible personal property which is used only for research medical scientific or technical activities (eg microscopes x-ray machines surgical instruments and spectrometers) and which has a useful life of
more than two years and an acquisition cost of $1000 or more per unit)
1H2(a)(3) Approvals
In furtherance of the foregoing the Grant Officer does hereby provide approval for the following purchases which shall not be
construed as authorization to exceed the total estimated amount
or the obligated amount of this Grant whichever is less (see
Section 1C above)
- one microscope - two laboratory refrigerators
6)
PCE-5053-G-00-3045-00 Page 11
- two laboratoryfreezers - one diesel engine
one water pump and associated electrical materials-
IH2(a)(4) EMueption for Automation Eguipment
for the purchase of automation equipment which mayAny approval be provided in Section IH2(a)(3) above or subsequently
is not valid if the total cost of provided by the Grant Officer
purchases of automation equipment (eg computers word
related services made hereunder processors etc) software or
will exceed $100000 The Grantee must under such the Grant Officer for the
circumstances obtain the approval of any automation equipment software or total planned system of
related services
11 RESOLUTION OF CONFLICTS
the Attachments of this Grant shall beConflicts between any of
resolved by applying the following descending order of
precedence
Attachment 1 - Schedule - Standard ProvisionsAttachment 3
Attachment 2 - Program Description
Agency For International Development Research Grant Program
for Historically Black Colleges and Universities
Proposal Title Development and Dissemination of True Potato Seed Technology in Uganda
Institution Fort Valley State College Fort Valley Georgia 31030
Principal Investigator Dr A S Bhagsari Professor
AGENCY FOR INTERNATIONAL DEVELOPMENT RESEARCH GRANT PROGRAM
FOR HISTORICAL BLACK COLLEGES AND UNIVERSITIES
SUBMITTED BY FORT VALLEY STATE COLLEGE (FVSC) Agricultural Research Station 1005 State College Drive Fort Valley Georgia 31030-3298
PROPOSAL TITLE DEVELOPMENT AND DISSEMINATION OF TRUE POTATO SEED TECHNOLOGY IN UGANDA
PRINCIPAL INVESTIGATOR Ajmer SBhagsari Ph D (USA) Professor
Agricultural Research Station Fort Valley State College Fort Valley Georgia USA Telephone No (912) 825-6825 Fax No (912) 825-6376
CO-INVESTIGATOR Adipala Ekwamu PhD (Uganda) Faculty of Agriculture and Forestry
Department of Crop Science Makerere University Kampala Uganda Africa
DATE OF SUBMISSION April 1993
Signature of Principal Investigator Sgnature an Name
Agricultural Research Station Representative of Proposing Insfitution
Statements on Proposal Submission
This project proposal in part or in whole has not been submitted to any other1 funding agency and is not closely related to research being done for other sponsors
2 This project if approved will provide for its own logistic support and will not pose
any load on the USAID mission
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
PCE-5053-G-00-3045-00
Page 10
1H2 Ecuipment Purchases
Equipment purchases under this Grant must be made in accordance
with the Standard Provisions entitled Procurement of Goods and
Services and AID Eligibility Rules for Goods and Services
included in Attachment 3 of this Grant Inclusion of costs in
the budget of this Grant for the purchase of nonexpendable equipment does not obviate the requirements of Section J13 of
OMB Circular A-21 (for educational institutions) or Section 13
of Attachment B of OMB Circular A-122 (for nonprofit organizations other than educational institutions) for prior
approval of such purchases by the Grant Officer nor any other
terms and conditions of this Grant unless specifically stated
in Section lH2(a)(3) below
IH2(a) Reguirement for Prior Approval
Pursuant to Sections 1D3 and 1G3 above and the Standard
Provisions of this Grant entitled Allowable Costs and
Revision of Grant Budget and by extension Section 13 of
Attachment B of OMB Circular A-122 the Grantee must obtain AID Grant Officer approval for purchases of the following
1H2(a)(1) General Purpose EQuipment which is defined as an
article of nonexpendable tangible personal property which is
usable for other than research medical scientific or technical
activities whether or not special modifications are needed to
make them suitable for a particular purpose (eg office
equipment and furnishings air conditioning equipment reproduction and printing equipment motor vehicles and
automatic data processing equipment) having a useful life of
more than two years and an acquisition cost of $500 or more per unit) and
IH2(a)(2) Special Purpose EguiPment which is defined as an
article of nonexpendable tangible personal property which is used only for research medical scientific or technical activities (eg microscopes x-ray machines surgical instruments and spectrometers) and which has a useful life of
more than two years and an acquisition cost of $1000 or more per unit)
1H2(a)(3) Approvals
In furtherance of the foregoing the Grant Officer does hereby provide approval for the following purchases which shall not be
construed as authorization to exceed the total estimated amount
or the obligated amount of this Grant whichever is less (see
Section 1C above)
- one microscope - two laboratory refrigerators
6)
PCE-5053-G-00-3045-00 Page 11
- two laboratoryfreezers - one diesel engine
one water pump and associated electrical materials-
IH2(a)(4) EMueption for Automation Eguipment
for the purchase of automation equipment which mayAny approval be provided in Section IH2(a)(3) above or subsequently
is not valid if the total cost of provided by the Grant Officer
purchases of automation equipment (eg computers word
related services made hereunder processors etc) software or
will exceed $100000 The Grantee must under such the Grant Officer for the
circumstances obtain the approval of any automation equipment software or total planned system of
related services
11 RESOLUTION OF CONFLICTS
the Attachments of this Grant shall beConflicts between any of
resolved by applying the following descending order of
precedence
Attachment 1 - Schedule - Standard ProvisionsAttachment 3
Attachment 2 - Program Description
Agency For International Development Research Grant Program
for Historically Black Colleges and Universities
Proposal Title Development and Dissemination of True Potato Seed Technology in Uganda
Institution Fort Valley State College Fort Valley Georgia 31030
Principal Investigator Dr A S Bhagsari Professor
AGENCY FOR INTERNATIONAL DEVELOPMENT RESEARCH GRANT PROGRAM
FOR HISTORICAL BLACK COLLEGES AND UNIVERSITIES
SUBMITTED BY FORT VALLEY STATE COLLEGE (FVSC) Agricultural Research Station 1005 State College Drive Fort Valley Georgia 31030-3298
PROPOSAL TITLE DEVELOPMENT AND DISSEMINATION OF TRUE POTATO SEED TECHNOLOGY IN UGANDA
PRINCIPAL INVESTIGATOR Ajmer SBhagsari Ph D (USA) Professor
Agricultural Research Station Fort Valley State College Fort Valley Georgia USA Telephone No (912) 825-6825 Fax No (912) 825-6376
CO-INVESTIGATOR Adipala Ekwamu PhD (Uganda) Faculty of Agriculture and Forestry
Department of Crop Science Makerere University Kampala Uganda Africa
DATE OF SUBMISSION April 1993
Signature of Principal Investigator Sgnature an Name
Agricultural Research Station Representative of Proposing Insfitution
Statements on Proposal Submission
This project proposal in part or in whole has not been submitted to any other1 funding agency and is not closely related to research being done for other sponsors
2 This project if approved will provide for its own logistic support and will not pose
any load on the USAID mission
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
PCE-5053-G-00-3045-00 Page 11
- two laboratoryfreezers - one diesel engine
one water pump and associated electrical materials-
IH2(a)(4) EMueption for Automation Eguipment
for the purchase of automation equipment which mayAny approval be provided in Section IH2(a)(3) above or subsequently
is not valid if the total cost of provided by the Grant Officer
purchases of automation equipment (eg computers word
related services made hereunder processors etc) software or
will exceed $100000 The Grantee must under such the Grant Officer for the
circumstances obtain the approval of any automation equipment software or total planned system of
related services
11 RESOLUTION OF CONFLICTS
the Attachments of this Grant shall beConflicts between any of
resolved by applying the following descending order of
precedence
Attachment 1 - Schedule - Standard ProvisionsAttachment 3
Attachment 2 - Program Description
Agency For International Development Research Grant Program
for Historically Black Colleges and Universities
Proposal Title Development and Dissemination of True Potato Seed Technology in Uganda
Institution Fort Valley State College Fort Valley Georgia 31030
Principal Investigator Dr A S Bhagsari Professor
AGENCY FOR INTERNATIONAL DEVELOPMENT RESEARCH GRANT PROGRAM
FOR HISTORICAL BLACK COLLEGES AND UNIVERSITIES
SUBMITTED BY FORT VALLEY STATE COLLEGE (FVSC) Agricultural Research Station 1005 State College Drive Fort Valley Georgia 31030-3298
PROPOSAL TITLE DEVELOPMENT AND DISSEMINATION OF TRUE POTATO SEED TECHNOLOGY IN UGANDA
PRINCIPAL INVESTIGATOR Ajmer SBhagsari Ph D (USA) Professor
Agricultural Research Station Fort Valley State College Fort Valley Georgia USA Telephone No (912) 825-6825 Fax No (912) 825-6376
CO-INVESTIGATOR Adipala Ekwamu PhD (Uganda) Faculty of Agriculture and Forestry
Department of Crop Science Makerere University Kampala Uganda Africa
DATE OF SUBMISSION April 1993
Signature of Principal Investigator Sgnature an Name
Agricultural Research Station Representative of Proposing Insfitution
Statements on Proposal Submission
This project proposal in part or in whole has not been submitted to any other1 funding agency and is not closely related to research being done for other sponsors
2 This project if approved will provide for its own logistic support and will not pose
any load on the USAID mission
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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23
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49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
Agency For International Development Research Grant Program
for Historically Black Colleges and Universities
Proposal Title Development and Dissemination of True Potato Seed Technology in Uganda
Institution Fort Valley State College Fort Valley Georgia 31030
Principal Investigator Dr A S Bhagsari Professor
AGENCY FOR INTERNATIONAL DEVELOPMENT RESEARCH GRANT PROGRAM
FOR HISTORICAL BLACK COLLEGES AND UNIVERSITIES
SUBMITTED BY FORT VALLEY STATE COLLEGE (FVSC) Agricultural Research Station 1005 State College Drive Fort Valley Georgia 31030-3298
PROPOSAL TITLE DEVELOPMENT AND DISSEMINATION OF TRUE POTATO SEED TECHNOLOGY IN UGANDA
PRINCIPAL INVESTIGATOR Ajmer SBhagsari Ph D (USA) Professor
Agricultural Research Station Fort Valley State College Fort Valley Georgia USA Telephone No (912) 825-6825 Fax No (912) 825-6376
CO-INVESTIGATOR Adipala Ekwamu PhD (Uganda) Faculty of Agriculture and Forestry
Department of Crop Science Makerere University Kampala Uganda Africa
DATE OF SUBMISSION April 1993
Signature of Principal Investigator Sgnature an Name
Agricultural Research Station Representative of Proposing Insfitution
Statements on Proposal Submission
This project proposal in part or in whole has not been submitted to any other1 funding agency and is not closely related to research being done for other sponsors
2 This project if approved will provide for its own logistic support and will not pose
any load on the USAID mission
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
AGENCY FOR INTERNATIONAL DEVELOPMENT RESEARCH GRANT PROGRAM
FOR HISTORICAL BLACK COLLEGES AND UNIVERSITIES
SUBMITTED BY FORT VALLEY STATE COLLEGE (FVSC) Agricultural Research Station 1005 State College Drive Fort Valley Georgia 31030-3298
PROPOSAL TITLE DEVELOPMENT AND DISSEMINATION OF TRUE POTATO SEED TECHNOLOGY IN UGANDA
PRINCIPAL INVESTIGATOR Ajmer SBhagsari Ph D (USA) Professor
Agricultural Research Station Fort Valley State College Fort Valley Georgia USA Telephone No (912) 825-6825 Fax No (912) 825-6376
CO-INVESTIGATOR Adipala Ekwamu PhD (Uganda) Faculty of Agriculture and Forestry
Department of Crop Science Makerere University Kampala Uganda Africa
DATE OF SUBMISSION April 1993
Signature of Principal Investigator Sgnature an Name
Agricultural Research Station Representative of Proposing Insfitution
Statements on Proposal Submission
This project proposal in part or in whole has not been submitted to any other1 funding agency and is not closely related to research being done for other sponsors
2 This project if approved will provide for its own logistic support and will not pose
any load on the USAID mission
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
TABLE OF CONTENTS
Page
Need for the Proposed Research 1
Tim e Phased Plan of W ork 16amp17
A Brief Discussion of the Problem 2
Emerging TPS Technology 2
Pertinence of Proposed Research 3
Discussion of the Broad Objectives 4
Brief Statement of Projected Output 5
HBCLs Institutional Characteristics and International Capabilities 6
Collaborative MechanismsNetwork 7
Scientific Aspects of Proposed Research 8
Statement of the Research Question to be Investigated 8
References Supporting the Scientific Feasibility 9
Potential of the Proposed Research II
Innovative Character of the Research 11
Description of Experimental Design Methodologies and Techniques 12
Logistic Feasibility and Requirements for Implementing the Research 18
Budget Inform ation 19
Literature Cited 20-24
Curriculum Vitae of Investigators and Cooperators 25
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
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22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
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26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
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30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
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32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
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34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
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23
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47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
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49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
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24
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63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
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2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
Title Development and Dissemination of True Potato Seed Technology in Uganda
II Need(s) for the Proposed Research
The potato (Solanum tuberosum L) one of the most valuable staple food crops for
humankind is generally superior in energy and protein production per unit area and potato
proteins are also rich in essential amino acids (12) Among food crops it ranks fifth in area and
fourth in production (1)and currently it is cultivated in about 130 countries where three-fourth of
the world population live (3) Although potato is considered a cool season crop for temperate
climates (45) and highland tropics (HLT) its production is now extending to warm climates in the
lowland tropics (LLT) as well as subtropical environments (678910) The rate of potato production
and consumption in developing countries has exceeded most other food crops (211)
The potato is now recognized as an important food and cash crop in Uganda particularly
on subsistence farms in the highlands The highland climate (altitude 1500-2000 m with mean
temperatures 15-16 C and bimodal rainfall 120-150 cm) is suitable for raising two and even three
potato crops per year Main potato growing areas in the highlands are concentrated in the Kigezi
and Ruwenzori Mountains in southwest Uganda and Mbale in the lower slopes of Elgon Mountain
in the east
Potato production isalso becoming popular in LLT situated 1200 to 1500 m above sea leel
(asl) with mean temperature of 21 to 24 C and rainfall of 120 to 150 cm However potato yields
are lower in LLT due to the prevalence of many diseases insects and pests (121314) Imported
potato germplasm played a vital role in potato development in the US and Canada (15) The
than 40 tha in Europeintroduction of technology has increased current potato yields to more
otherwise yields would have ranged from 10 to 15 tha (16) quite similar to that in the tropics (12)
With the adoption of suitable technology best potato yields averaged 1000 tha in the US (17) and
a similar yield was reported from microplots in the HLT in Uganda t18) With the introduction of
technology food crop yields can increase 2 to 3 times the current levels (219)
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
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10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
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16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
2
1 A Brief Discussion of the Problem
Uganda has the finest natural resources in sub-Saharan Africa but these remain unexploited
(2021) Due to loss of foreign exchange earnings from coffee many food crops are grown for
export purposes which in turn is causing internal food shortages resulting in malnutrition (19)
Increased potato production in the HLT and expanding production in the LLT can supplement the
internal food supply to offset shortages caused by export of food crops and a 34 increase in
population (1921)
From 1970 to 1984 political unrest caused crop germplasm losses including potato
throughout Uganda With assistance from the International Potato Center (CIP) and USAID potato
germplasm is imported and evaluated for adaptation only in the HLT Generally cultivars respond
differently in the LLT and HLT Cruza a high yielding potato cultivar in the HLT in Uganda (18)
failed to form tubers in the LLT (22) Further due to high population density and shortage of land
potatoes are planted in the HLT without rotation on steep slopes causing soil erosion The main
marketing center Kampala City- situated in the LLT is far away from the highlands and transport
costs are charged in the price to consumers Therefore it would be more cost effective to produce
potatoes in the LLT as well The current narrow germplasm base needs expansion through
introductions of adapted cultivars from North America Europe and Asia In the absence of
temperature controlled storage facilities and dormancy releasing chemicals only short dormancy
cultivars are popular because in biomodal rainfall areas potato seed can be used for planting
successive crops (1822) However in tropical and subtropical regions high aphid populations and
year-round presence of diseases make it difficult for those countries to maintain their own certified
seed (223)
Emerging TPS Technology
Because of multiple problems associated with potato production from seed tubers in warm
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
3
climates the use of true potato seed (TPS) as planting material is under investigation
(1424252627) and holds great potential in tropical cnvironment (23282930) Currently the
open-pollinated TPS is planted because hybrid seed production through hand pollination is
expensive (2331) The open-pollinated progenies usually yield less than hybrid progenies
(2432333435) Hybrid potato seed production appears feasible through natural cross pollination
due to the discovery of female fertile and male sterile cultivars such as cv Atlantic and some others
(3536)
Since tetraploid potatoes can be self-fertile it would be preferable to use potato lines which
are female fertile but male sterile as the female parent This would eliminate the need to hand
emasculate the buds prior to pollination and also eliminate the effect of inbreeding depression as
a result of selfing As a first step in developing this proposal lines need to be identified that are
either female fertile and male sterile or female sterile and male fertile when grown under high
temperature conditions Several lines are known to be extremely female fertile and nearly male
sterile like Atlantic Coastal Russet Belrus and numerous breeding lines from the USDA Beltsville
potato breeding program and most hybrids from 4x-2x crosses Many other lines are known to be
extremely male and female fertile Coastal Chip Superior and numerous breeding lines from the
USDA Beltsville potato breeding program A much smaller number of breeding lines from the
USDA Beltsville program are known to be male fertile with very reduced female fertility (Dr
Haynes 1993 personal communication)
2 Pertinence of Proposed Research to a Possible Solution
Quality seed is critical to potato production in a tropical environment (41437) In the LLT
clonally propagated potatoes degenerate rapidly and yields decline unless seed is imported from cool
climates for each crop (14) This practice is expensive and beyond the means of limited-resource
farmers in Uganda The use of TPS in potato production reduces costs for seed and transportation
7
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
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6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
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9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
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Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
4
and decreases the incidence of potato diseases (2) For exploiting hybrid vigor female fertile male
sterile and male fertile genotypes will be identified for TPS production for raising seedling tubers
with yield potential similar to potatoes produced from seed tubers imported from cool climates
Further this research would attempt to identify short dormancy cultivars suitable for planting
successive crops without the need for long storage periods or to the use of dormancy release
chemicals The scientific foundation and requisite technology for TPS seedling tuber production will
be developed in the LLT Limited-resource farmers will benefit from the diffusion of TPS technology
and consumers will pay less because of local availability of the potato
3 How specifically the Projects Results Contribute to the Solution of Developing Country
Program and AIDs Mission
Lack of locally available technological inputs scientific effort low productivity and the
resulting poverty and malnutrition are some of the main problems facing Uganda and the AID is
addressing all of them (1921) About 22 million limited-resource farmers with holding size of 2
to 3 ha produce 94 of the total agricultural output in the country (21) The continuous civil unrest
has led to a brain drain and reduction in agricultural production The USAID sponsored project
Manpower for Agricultural Development has a strong commitment to food security and seed
supply in Uganda This planned project would address AIDs mission in alleviating seed problems
in an important food and cash crop The development of TPS technology will enhance potato
production in the LLT and in asimilar climatic zone elsewhere The cheap and easy availability of
TPS seedling tubers to farmers and home gardeners will enhance potato production and improve
quantitative and qualitatively aspects of nutrition
4 Discussion of the Broad Objective to be Achieved by Research
a To determine the yield potential of newly introduced and adapted germplasm in the
LLT of Uganda
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
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6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
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11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
5
b To determine the effect of altitude and photoperiod on flowering berry development
and seed quality of potato germplasm adapted to the tropical environment
c To study the effect of trellises and supplemental nitrogen (in excess of that used for
tuber production) applied during seed development on 100 - TPS weight seedling
vigor and quality
d To determine the effect of seed priming on germination and seedling vigor
e To initiate clonal seed tuber degenerative studies to determine yield decline resulting
from the continuous use of seed tubers (without renewal) in the LLT
f To compare the yield potential of TPS seedling tubers (G) produced in the HLT and
LLT and rate of decline in yield in successive cropping
g To identify male fertile potato lines through in vitro techniques of pollen germination
and pollen tube gr-o1h
h To evaluate the combining ability of female fertile and male fertile potato genotypes
i To study the feasibility of multiple potato cropping three potato crops a year and
intercropping with maize
S Brief Statement of Projected Output
By the completion of this proposed project 40 to 50 well adapted genotypes from the tropics
and subtropics would have been evaluated and their susceptibility to insects resistance to diseases
and yield potential determined The flowering and seed production response of this germplasm to
this photoperiod and altitude will also be known The effects of chemicals and hormones for
inducing TPS production and accelerating seed germination will be identified Identification of
separate female and male fertile genotypes will impart capability to produce hybrid TPS naturallN
cross pollinated under field conditions making TPS a viable option in the tropics The rate of
7
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
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2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
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11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
6
decline and degeneration caused by viruses in TPS seedling tuber and clonal seed tuber yields will
be evaluated A package of practices for TPS and TPS seedling tuber production will be prepared
for dissemination through the extension agents Finally an ideal location for TPS production similar
to the one at Osrone Chile contracted by CIP to produce TPS will be identified and become the
focal point for TPS technology development in tropical Africa
Ill HBCUs Institutional Characteristics and International Capabilities Related to Proposal
The International activities of the Fort Valley State College are handled through its Office of
International Programs which in turn coordinates its activities with the College Deans and the
Office of Vice-President for Academic Affairs The approval for submitting this proposal rests with
the President but subsequent implementation of the approved proposal will be under the supervision
of the Dean School of Agriculture Home Economics and Allied Programs
The Fort Valley State College has been involved in international activities since 1979 and
is a charter member of the Southeast Consortium for International Development (SECID) Since then
it has assigned one or two scientists to international projects in various countries such as the
Dominican Republic Guinea Burkina Faso Mali India and Uganda Furthermore this institution
has signed MOUs for joint international activities with the Dominican Republic Haiti Ohio State
University and Winrock International The college administration is fully committed to its
international mission and encourages its faculty staff and students to participate in international
activities
9O
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
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11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
IV Collaborative MechanismNetwork
UniversityInstitution
Agri Res Station Fort Valley State College Fort Valley GA 31030
United States Dept Agri Agricultural Res Service Vegetable Laboratory Plant Sciences Institute Beltsville Maryland 20705
Makerere University Kampala Uganda Dept of Crop Science Faculty of Agric amp Forestry
Ministry (if Agricullure Govt of Uganda
Central Potato Institute Lima Peru Regional Officers East Africa Kenya and Uganda
Person and Position
Dr A S Bhagsari Professor Agronomy
Dr K G Haynes Research Plant Geneticist Potato
Dr Adipala Ekwamu Assistant Professor (Lecturer)
Mr D Akimanzi (Assisted by C R Kainzikwera) National Potato
Development Officer Entebbe
Dr H M Kidanemariam Dr L Sikka (CIP Scientists)
Time devoted
10
As needed
10
5
As Needed
Task description
Lead person project development and execution germplasm procurement TPS production and coordination at all locations
Potato germplasm resource person training and advice Make preparation for special crosses for TPS at Beltsville Presque Isle Maine Fort Valley GA and Uganda
Lead person at the Makerere Uni guide two graduate students for TPS production technology in vitro techniques for pollen germination and growth
Lead person for potato germplasm procurement and quarantine Maintenance of potato genotypes at the Highland Crop Res Center Kalengyere
Germplasm Resource Scientists
Resources Committed
Two greenhouses headhouse and laboratory space and field facilities for TPS production and testing
Greenhouse space for specific TPS production crosses In vitro techniques to determine pollen germination amp pollen tube growth _
Laboratory space at Makerere University field plot and greenhouse facilities at Kabanyolo Agric Res Institute
Farm facilities greenhouse and laboratory space at the Highland Crop Res Center Kalengyere
Maintenance of special selected lines needed for this project
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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21
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17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
8
V SCIENTIFIC ASPECTS OF PROPOSED WORK
1 Statement of the Research Question that isto be Investigated
Exotic germplasm adapted to warm climates and possessing short dormancy will be evaluated Since
flowering and fruiting are aprerequisite for this technology asearch for parental lines having profuse flowering and
high pollen fertility will be undertaken Using the pollen tube growth technique (3638) separate female fertile and
male fertile genotypes will be identified to produce hybrid seed through natural cross pollination The cross
showing the best combining ability will be used to produce TPS which will be used to produce first generation
seedling tuber (G) and again multiplied to produce second generation seedling tubers (G2) for evaluation in the
LLT Quality of the seed and seedling tubers and its relationship to potato yield will also be studied Finally
agronomic and physiological studies will be conducted for TPS technology development
2 Providing Information additive to existing knowledge in published literature or other sources
Limited information for potato production relevant to tropical Africa is available in the published literature
The TPS program though known in China since 1960 (26) was initiated in 1978 by CIP scientists in collaboration
with some developed countries to introduce the potato in warm climates (6) Practically no information isavailable
in the literature about flowering berry production and quality of seed in tropical Africa Identification of separate
male and female fertile genotypes and their use in the production of hybrid TPS through natural cross pollination
would be new knowledge for the tropics New information regarding suitability of G G2 seedling tubers will P
developed The combination of environmental factors impacting TPS production will be new information for the
African environment
3 Description of Expected Causal Relationships and Hypothesis Statement
Vitality and yield potential of potato seed tubers decreases during successive cropping in hot climates due
to degenerative disease caused by viruses Disease transmission (except potato spindle tuber viroid) by TPS is less
than by seed tubers Use of new adapted germplasm from environments similar to the lowlands in Uganda will
minimize seed tuber degeneration when TPS technology isdeveloped and adopted We hypothesize that the yield
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
9shy
potential of TPS seedling tubers (G and G2) produced under the lowland conditions is similar to that obtainable
from clonally propagated seed tubers imported from cool climates
4 References Supporting the Scientific Feasibility
Potato tuber yields of 100 tha are predicted (16) and are obtainable (17) in temperate climates Production
of potatoes isconstrained by a lack of quality planting material in the LLT but TPS can provide healthy amp low cost
planting material to produce seedling tubers (14242830313940) Potato production from TPS can be done by
1 Direct seed sowing in the field 2 Raising seedlings in nursery beds for transplanting and 3 Production of
seedling tubers (G) from TPS for next planting
Potato Production from Direct TPS Planting
Direct field sowing in temperate climates with long growing seasons ispossible (31) but in hot climates seed
Directgermination is low (2941) Yields from TPS sown directly and from transplant seedlings were similar (39)
seeded TP$ F (hybrids) yielded less than 25 of clonally propagated Russet Burbank at 141 days after planting
(DAP) but at 195 DAP yields were similar and ranged from 54 to 62 tha (42) The optimum density for direct sown
TPS isabout 96 plantsm yielding 651 to 76 Kg tubersm (39) Thus short rainy seasons in Uganda will preclude
the possibility of potato production from direct field planted TPS
Potato Production from Transplanting
The use of TPS for producing transplants for raising disease free tubers is feasible (2931424344) and TPS
transplant production techniques are similar to small seeded vegetable production (2931) and hold great potential
for developing countries (31) The yield from a transplanted crop is 200 to 500 higher than that from direct
climate potato yield from an open pollinated (OP) TPS transplant crop was 146 thaseeded TPS (31) In a warm
about 460 higher than the national average yield (14) Yield from transplants ranged from 360 to 410 tha (45)
Yield for OP-TPS transplants ranged from 110 to 240 tha (27) and 234 to 310 tha (33) in the HLT and temperate
climates respectively The yield from transplants of OP-TPS was lower than that of hybrid-TPS (23273246) Vine
growth was faster for seed tuber propagated crops than for TPS transplants which produced more auxiliary branches
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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21
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3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
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32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
10
per stem (47) The transplants stored for a week at about 7 C showed better survival than those maintained at 12C
(44) but planting in soil at 30 C caused significant willing (48)
Potato Production from Seedling Tubers
Disease free seedling tubers car jced from TPS for use as regular seed (1423) A similar technique
in China improved potato yields from 60 to 20 tha (26) Hybrid TPS families and seed tuber propagated cultivars
gave higher yield than OP-TPS (3442) but TPS use is recommended since high yielding OP-TPS families can be
identified (35) The highest yields for seedling tubers (G) for selection CFK-691 and for cv Ackersegan were 239
tha and 148 tha respectively (49) Seedling tubers (G) yielded 470 to 550 tha in the HILT and this yield was
significantly higher than that of transplanting (45) The size of the seedling tubers also impacts yield The potato
yields for seedling tuber sizes of 3 to 5 g and 25 to 50 g ranged from 200 to 300 tha and 300 to 490 tha
respectively (50) The yields for seedling tubers (G) ranged from 233 to 316 tha at 1330 m a s I and 71 to 147
tha at 800 m a s 1(27) Yields from TPS seedling tubers were similar to clenal tubers (2739) But Li (51) indicated
that seedling tuber yields were 35 to 55 higher than the control planted with local seed tubers
Flowering Fruiting and Seed Production
True potato seeJ production on a commercial scale depends upon the flowering characteristic of parental
material (52) Environmental factors especially the quality and quantity of light temperature and plant hormones
can modify potato flowering response but generally 15 to 16 hrs exposure to light will induce flowering (13) I
the LLT with short photoperiod only 2 out of 47 potato cultivars studied formed berries and produced seed u~der
the natural conditions (22) Photoperiod longer than 12 hrs and warmer night temperature promoted flower
production and reduced irradiance had the opposite effect (53) Treatment of flower buds at an early stage with
silver thiosulphate increased flower retention of cv Katahdin over that of control plants (54) In general higher
altitude (2521 m a s I) and 14 to 16 hrs photoperiod promote blooming and high berry set A high doze of
supplemental nitrogen also improved seed quality (5255) Berry size and 100-seed weight can be increased by
decreasing number of flowers per inflorescence and by pollination of the stigma (52) Time for berry ripening
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
11
Berries harvested 1I depends upon the environment and the genotype but ripening is delayed in cooler climate
weeks after pollination produce vigorous seed (56)
Seed Dormancy Seed Storage and Seed Quality
Diploids lose dormancy more rapidly than tetraploids (58)Genetic variations exist in TPS dormancy (57)
Under cool and dry conditions TPS can be stored for 10 to 25 years without loss in seed viability (596061) Seed
can be broken by soaking TPS in 2000 ppm gibberellic acid for 24 hrs (62)dormancy
The use of TPS Currently TPS technology research is focused on the production of high quality seed (55)
with optimum vigor is important for seedling performance under sub-optimal farming conditions (3757) and
is generally low during early emergence (63) Seed quality can be improved by properseedling vigor of TPS
at - 10 nutrition during TPS production (525564) The soaking of TPS in a solution consisting of KNO 3 + K3PO
MPa promoted faster emergence and dry matter accumulation (63) A significant positive correlation was reported
- seed weight and harvest index (65) and between seedling vigor and yield (42)between 1000
5 Potential of the Proposed Research
The possibility exists that more separate female fertile genotypes and good male fertile pollinators may be
identified to produce hybrid seed for developing TPS technology Atlantic aknown female fertile cultivar (36) holds
a great potential for the LLT as it had the highest photosynthetic rate leaf area index photosynthate partitioning
help avoid disease related cropand yield in a warm climate (66) The use of hybrid seed and even OP-TPS can
losses or even crop failures The development of the potato in the LLT can be very important in alleviating hunger
and improving nutrition in Uganda and the surrounding six countries
6 Innovative Character of the Research
In the traditional system development of a potato cultivar and maintenance of disease free seed is most
expensive and seed alone accounts 50 to 75 of production costs (2424) The introduction of potato in the lower
This research will attempt to develop the capacity to produce hybrid TPS tropics is an innovation by itself (14)
The in vio approach to identify separate genotypes having completely female using adapted cultivars in the LLT
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
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9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
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12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
12
fertile and male fertile flowers or partial degrees of male and female fertility and sterility for solving an important
problem in TPS technology development is very unique Another unique feature of this research involves testing
continous potato cropping by having two crops in the two rainy seasons and a third dry season crop with
supplemental irrigation from streams in the valleys
7 Experimental Design Methodologies and Techniques [For objective a-i pages 4-5]
a Potato Germplasm About 50 well adapted potato genotypes with known high productivity from
warm climates and the LLT will be obtained from the US and CIP germplasm resources and
introduced in Uganda following quarantine pr-x)edures The tentative list of the genotypes includes
LT4 DTO-2 (10) Spunta Atica Desiree Clautar (7) DTO-33 DTO-28 Katahdin LT2 (53) LT5
Cosima Sequoia (67) Michinac and selection Wn C316-1 (42) Red Pontiac (67) Cardinal
Patrones Multa Bintje Achat Baraka and Radosa (14) and other important cultivars identified by
(6182267) All the new germplasm will be tested in single-row or three-row plots (depending
upon the quantity of the seed tubers) in the HLT and LLT of Uganda before initiating flowering and
seed production experiments The rows will be 6 m long and 075 m wide with 03 m spacing
between plants The experiment will be randomized complete block with four replications Cultural
practices to be followed were described elsewhere (1822)
b This pot experiment will involve 15 to 20 well adapted and productive genotypes planted at three
altitudes that is Kalengyere (2500 masl) Bunginyana (2000 m) and Kabanyolo (1250 m)
Photoperiods of 12 hrs (natural day light) and 16 hours (obtained with supplemental light) will be
used Seed tubers will be planted in pots five pots per genotype and replicated four times
Fertilizer will be applied pre-and post-planting and irrigation and insecticides will be applied as
needed Vines will be supported on trellises Flower initiation flower buds per inflorescence
number of berries and quantity of seed produced ill be recorded Berries will be harvested about
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
13shy
4-6 weeks after pollination and ripened for 2-4 weeks at room temperature before seed extraction
The berries will be crushed and fermented at room temperature for 48 hrs before seed separation
The seed will be soaked for 10 min in a 05 solution of sodium hypochlorite followed by rinsing
The seed will be dried at 20 Cand stored over silica gel Before use the seed will be soaked for 24
hrs in GA (concentration 1000-2000 PPM) to break the dormancy as described by other researchers
(4355576263)
Germination tests will be performed in Petri dishes on lots of 100 seed for each genotype using four
replications Seeds will be placed on a filter paper and moistened with 5 ml water Seedling
emergence tests will be conducted in a greenhouse in wooden trays containing soil and sand 11
ratio by planting 20 seed per genotype in randomized complete block design replicated four times
The rows will be 30 cm x 6 cm Plant emergence will be evaluated up to 20 days Fresh and dry
matter accumulation of seedlings cut at soil level will be determined about 20 DAP Velocity of
germination will be calculated (57)by the equation
Coefficient of velocity (CGV) - 100 x A + A2 - A
AIT 1 + A2T2 -- Aj
When A - number of seeds germinated and
When T - days from sowing corresponding to A
c Two field experiments will be conducted one each for the lowland and highland tropics by using
two well adapted potato genotypes (Piratini and CIP 3813799) There will be two trellises
ireatments and three nitrogen treatments (0 150 and 300 kg Nha) The experiment will be split-split
plot with four replications Plants will be grown with 16 hrs photoperiod in rows 6 m long with
1 m spacing between rows and plants Insecticides and irrigation will be given as needed Berry
collection seed extraction drying storage and dormancy breaking procedures will be as described
above in item (b) The seed will be tested at one 3 and 6 months after extraction for germination
coefficient of velocity of emergence and seedling vigor in a separate experiment with four
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
14
replications as described in item (b) above
d For this objective OP-TPS duced in items (b) and (c) will be utilized Three seed priming
treatments will be imposed on each type of seed By running preliminary experiments optimum
concentration of priming treatments will be determined for use as part of the experimental
treatments Pre-sowing (priming) treatments will comprise of GA (1500 PPM) soaking for one day
KN03 + K3PO4 at - 10 MPa for five days and just water rinsing of TPS (control) About 100 seeds
per treatment will be placed on Whatman paper in Petri dishes and soaked in the respective
solutions 5 ml per dish The primed seed lots will be again dried separately at about 15 C for two
days Further these dried seeds will be stored five more days over silica gel at room temperature
before initiation of tests Percent germination coefficient of velocity of emergence and seedling
vigor will be determined as described in other reports (555763)
e Potato seed tubers produced in item (a) in the HLT will be planted in the LLT by following practices
as previously described (22) These seed tubers after storing in cold storage and at room
temperature will be used in successive croppings that is two crops a year Dormancy releasing
chemicals such as rendite arid bromoethane will be used before planting if needed
f Seedling tubers will be produced in raised nursery beds by using locally produced TPS romten
genotypes Farm yard manure sand and soil are mixed to construct raised beds which are provided
slight shade during early stages of seed germination About 400 seeds m2 are planted and fter
germination are thinned to 70 plants M2 Details for raising of seedling tubers were previously
described (18) Using these seedling tubers (G) replicated field trials will be conducted in the LLT
in successive cropping without seed renewal
g For in vitro pollen germination and pollen tube grovih flowers will be harvested every other day
over three weeks aiound 800 am brought to the laboratory and held for 1 hour at room
temperature before the pollen is removed and tested Pollen viability will be determined on pollen
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
samples taken from single flowers using the in viro germination test described earlier (3638) In
brief 14 ml of freshly prepared germination medium containing 20 cc-lactose (wv) and 50 ppm
boron pH 52 are placed in 35 mm petri plates Pollen from single flowers is spread over the
surface of the medium by touching the anthers of the flower for 2 seconds with a mechanical
vibrator The plates are covered and held in an incubator at 27 C Viability is determined by
scoring percent normal and total (normal plus abnormal) germination at 2 hours from initiation of
germination using aDiaphot-TMD inverted microscope (Nikon Image Systems Columbia MD) and
a light source from a halogen lamp A pollen grain is considered to be germinated when the length
of the pollen tube reached the diameter of the grain or longer Each determination consisted of
counting about 600 pollens by examing 8 to 10 locations in a series of parallel traverses across the
petri plate that contained the sample under test
About forty potato genotypes will be evaluated for female and male fertility in replicated field trials
Pollen fertility will be determined by following the procedure of Abdul-Baki and Haynes (36) as
described under (g) Female fertility will be determined by the presence or absence of fruit 3-4
weeks after flowering Both male and female fertile genotypes with known performance will be
grown in a greenhouse under 16 hrs photoperiod and will be intercrossed using coirirolled
pollination Emasculation will be done prior to pollen dehiscence and pollen from the male parent
will be placed on the stigma and the flower cluster will be tagged with parentage Upon ripenig
berries will be collected for seed extraction and preparation for subsequent planting Seed from such
hybridization will be evaluated in replicated field trials for yield uniformity and tuber conformation
The resulting best female fertile and male fertile combination will be used in TPS production by
natural cross pollination This TPS will form the basis for seedling tuber production IG) and
subsequent evaluation
Experiments will be conducted using potential potato genotypes under irrigated conditons during the
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
16
dry season Replicated trials of potato and maize intercropping will also be conducted The cultural
practices and techniques were described earlier (1822)
8 Time-Phased Plan of Work
Note Three months lead time will be needed Mr Akimanzi will supervise HLT research Dr Adipala will
advise two graduate students involved in this research proposal and conduct research in the LLT assisted
by tvo technicians and hired help
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
Period
Oct 1993 - Dec 1993
Jan 1994 - June 1994 First Rainy Season (Potato Crop I)
July 1994 - Dec 1994 Second Rainy Season (Potato Crop II)
Jan 1995 - June 1995 First Rain Season (Potato Crop Itt)
July 1995 - Dec 1995 (Potato Crop IV)
17-
Activity
Planning and organizing phase securing potato germplasm placing orders for equipment buying chemicals and supplies and obtaining TPS from CIP
HLT - Expts for adapted germplasm evaluation effect of photoperiod and altitude on TPS production effect of trellises and suppiemental nitrogen on TPS
LLT - Expts to determine effect of photoperiod and altitude on TPS production plant Atlantic and Coastal chip and others in a greenhouse to confirm female fertile and male sterile and female sterile and male fertile characters
HLT - Repeat all above expt from first rainy season produce seedling tubers (G) from TPS LLT - Repeat all expts from first rainy season prouuuce seedling tubers (G) from TPS Initiate tuber degenerative studies and seed priming vigor and quality work continue lab work for identifying genotypes with female and male fertility and sterility Make selective crosses between female fertile amp male fertile genotypes
HLT - Production of TPS seed multiplication for adapted genotypes production of more seedling tubers (G) from TPS testing G seedling tubers Testing of G tubers in 5 on-farm trials LLT - Plant second crop for degenerative studies plant seedling tuber (G) crop carry on seed priming seed quality work testing G tubers in 5 on-farm trials Plant male sterile and female fertile Atlantic and other male fertile and female sterile genotypes in the field to obtain hybrid seed Carry on lab identification studies for female and male fertility sterelity Evaluate hybrid TPS produced in selected crosses
HLT - TPS production from promising germplasm for LLT seedling tuber (G) production testing of G and G2 seedling tubers on the farm and 10 on-farm trials
LILT - Germination and seedling vigor studies field testing hybrid TPS produced from Atlantic as the female parent identification of male sterile and female fertile and female sterile and male sterile genotype through in vitro pollen germination and pollen tube growth Hybrid TPS production by using Atlantic as femal fertile and male sterile type Data analysis Manuscript preparation and publication
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
18 9 Logistic Feasibility and Requirements for Implementing the Research
This research will be conducted at the Highland Crop Research Center Kalengyere and at the Makerere
University Agricultural Research Institute Kabanyolo Recently both the research centers were renovated and
rehabilitated from the damage suffered during the civil unrest of 1980s through a grant from USAID to Ohio State
University with Fort Valley State College as the sub-contractor The author of this research proposal Dr A S
Bhagsari representing Fort Valley State College was the Technical Advisor to Crop Science Dept at the Makerere
University Highly motivated young scientists recently trained in the US are available to carry out the research
with small incentive pay to augment the low salaries The equipment and supplies needed will be provided through
this grant Main items to be purchased are two refrigerators one microscope one small diesel engine and a water
pump with flexible plastic hoses to set up irrigation for multiple (three crops a year) potato cropping potato
dormancy releasing chemicals (rendite) and hormones (GA3) These items are available in Kenya or may be shipped
from the U STransport will be available from the pool of vehicles donated by USAID when the gra1t terminates
in June 1993 The CIP scientists were very helpful in sharing potato cultivar and TPS germplasm with our team
during my stay in Uganda This project will be self sufficient and no burden to USAID mission in Uganda
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
19
VIII Budget Information
Item Description Host Country US
A Compensation Allowances amp Wages a Professor (Student Advisor) National potato leader two MSc
students and seed production specialist ($20000 per month for each five persons for 24 months)
$24000
b Two technicians at $150month 24 months $7200
c Labor 4 persons per day at $300 each for 100 weeks $6000
d Contract labor (field preparation) $2000
e Personal salaries 10 time (Dr Bhagsari) (24 months) Technician 10 time (Greenhouse potato seed production)
$11000 $4000
B Travel (Host Country Scientist) to the US a One visit each by one student (working on breeding) amp Professor
$6000
b Travel within host country $2000
c (US Personnel) One visit for germplasm arrangments 2 visits for crop planning management amp one visit by Dr Haynes for supervising potato breeding work
$10000
C Fringe benefits 25 $3750
D Overhead 291 $4365
E Equipment
a One microscope Diaphot-TmD Nikon Image System and related items
$3000
b c
2 Refrigerators and 2 freezers
Diesel engine water pump amp flexicable hose for potato plot
irrigation and electrical materials
$3000 $2000
F Expendable supplies
a Potato seed fertilizer insecticide (Chemical GA3 amp Rendite)
$5000 $685
b Glassware bags paper bags $2000
c Computer time and fees $1000
d Publications $1000
Total $66200 $33800
20
Literature Cited
1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
3 Mendoza H A 1989 Population breeding as a tool for germplasm enhancement Am Potato J66639-653
4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
5 Benoit G R C D Stanley W J Grant and D B Torrey 1983 Potato top growth as influenced by temperature Am Potato J 60 489-501
6 International Potato Center (CIP) 1983 Research for the potato in the year 2000 In W J Hook led) Proc Intl Congress 22-27 February 1982 CIP Lima Peru
7 Fahem M and A J Haverkort 1988 Comparison of the growth of potato crops grown in autumn and spring in North Africa Potato Res 31557-568
8 Manrique L A D P Bartholomew and E EEwing 1989 Growth and yield performance of several potato clones grown at three elevations in Hawaii I Plant Morphology Crop Sci 29363-370
9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
10 Midmore D J 1984 Potato (Solanum spp) in the hot tropics I Soil temperature effects on emergence plant development and yield Field Crops Res 8255-271
11 Horton D and R L Sawyer 1985 The potato as world food crop with special reference to developing areas p 1-34 In H L Paul (Ed) Potato physiology Academic Press Inc London (Ltd)
12 Nganga S 1964 The role of the potato in food production for countries in Africa p 63-68 S Nganga (ed) Potato development and transfer of technology in tropical Africa CIP
Lima Peru
13 Burton W G 1989 Propagation by true seed p 68-83 In W G Burton (Ed) The Potato Longman Scientific and Technical Longman House England
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
20
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1 Ross H 1986 Potato Breeding- Problems and Perspective p 1-132 In W Horn and G Robbelen (Eds) Advances in plant breeding Supplement 13 to Jof Plant Breeding Verlag Paul Parey D-2000 Hamburg 1 Germany
2 Zaag D FVan der and D Horton 1983 Potato utilization in world perspective with special reference to the tropics and sub-tropics p44-58 jn W L Hooker (Ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
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4 Swaminathan M S and RL Sawyer 1983 The potential of the potato as a world food p 3-4 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
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9 Manrique L A G Y Tsuji G Uehara and R L Fox 1984 Winter and summer performance of potato (Solanum tuberosum) in isohyperthermic regimes Am Potato J 6141shy56
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18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
21
14 Couto FA A LC Sikka and SP R Weerasinghe 1983 The potato in the lower tropics p 5-10 Ln W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
15 Plaisted R L and R W Hoopes 1989 The past record and future prospects for the use of exotic potato germplasm Am Potato j 66603-627
16 Vos J 1992 A Case History Hundred years of potato production in Europe with special reference to the Netherlands Am Potato ) 69731-751
17 Cho L Jand W M Iritini 1983 Comparison of growth and yield parameters of Russet Burbank for a two-year period Am Potato J 60569-576
18 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato production from true potato seed p 46 Proc Ninth Symposium of the Intl Soc for tropical root crops 20-26 Oct 1991 Accra Ghana
19 Acclerated food crop production strategy Uganda 1990 Ministry of Agriculture and Manpo er for Agricultural development p 1-82 Kampala Republic of Uganda
20 Annonymous 1985 Traditional Crop development in Sub-Saharan Africa p 1-35
21 Agenceny for Internatonal Development (AID) Washington DC 1988 Uganda Agricultural Non-Traditional Export Promotion Program (ANEPP) p 1-72
22 Bhags3ri A S L Sikka JM Ssebuliba and D R Akimanzi 1991 Evaluation of Irish Potato germplasm for stress-tolerance at low elevations in Uganda p 33 Proc Ninth Symposium of the Intl Soc for Root Crops 20-26 Oct 1991 Accra Ghana
23 Golmirzaie A Ni and H A Mendoza 1988 Breeding strategies for true potato seed production CIP Circular Vol 161-8
24 Sadik S 1983 Potato production from true seed-present and future p 18-25 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
25 Mendoza H A 1987 Advances in population breeding and its potential impact on the efficiency of breeding potatoes for developing countries p 235-245 In G JJellis and D E Richardson (Eds) The production of new potato varieties technological advances Cambridge University Press Cambridge
26 Song B Q D Yu and P Van der Zaag 1987 True potato seed in China Past present and future Am Potato J64 321-327
27 Zaag Van der P B Susana Z Ganga and S Gayao 1989 Field evaluation of true potato seed progenies in the Philippines Am Potato J66109-117
28 Malagamba P 1988 Potato production from true seed in tropical climates HortScience 23495-500
3S
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
22 29 Jaworski C A S C Phatak S R Ghate and RD Gitaitis 1988 Cultural practices in useof true seeds of potato and screening of tuber-forming Solanum species under hot climatic
condition HortScience 23500-504
30 Weerasinghe S P R 1983 The potato for the hot humid tropics p 183-184 In W LHooker (Ed) Research of the potato in the year 2000 Intl Potato Ctr Lima Peru
31 Martin M W 1988 Cultural practices for using true seed in potato production under temperate climates HortScience 23505-510
32 Kidane-Mariam H M G C Arndt A C Macaso-Khwaja and S J Peloquin 1985Comparisons between 4Xx2X hybrid and open-pollinated true-potato-seed families Potato Res 2835-42
33 Macaso-Khwaja A C and SJPeloquin 1983 Tuber yields of families from open pollinatedand hybrid true potato seed Am PotatoJ 60645-651
34 Veilleux R E and P D Relf 1983 Seed-propagated potatoes Explorer vs 4X-2X AmPotato J60790-792
35 Arndt G Cand SJ Peloquin 1990 The identification and evaluatin of hybrid plants amongopen pollinated true seed familities Am Potato J 67393-404
36 Abdul-Baki A A and K G Haynes 1993 Male fertility of derived tetraploids of Solanumtuberosum from groups tuberosurn X Phureia-Stenotomum Am Potato J (In Press)
37 Pallais N 1987 True potato seed quality Theor Applied Genet 73784-792
38 Abdul-Baki A A 1992 Determination of pollen viability in tomatoes J Amer Soc Hort Sci117473-476
39 Wiersema SG 1986 A method of producing seed tubers from true potato seed Potato Res 29 225-237
40 Wiersema SG and RCabello 1986 Comparative performance of different-sized seed tubers derived from true potato seed Am Potato ) 63241-249
41 Accatino P 1979 Agronomic management in the utiliztion of true potato seed Preliminaryresults p 61-99 Intl Potato Center Report of the planning conference on the production of potatoes from true seed Manila Philippines
42 Martin M W 198 Field production of potatoes from true seed and its use in breedingprogramme Potato Res 26219-227
43 Martin M W 1983 Techniques for successful field seeding of true potato seed Am Potato
J60245-259
44 Risse LA CA Jaworski SC Phatak and SR Ghate 1984 Effect of storage temperature
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
23
and duration on quality and survival of potato transplants Am Potato J 61261-265
45 Berrios D and PMalagamba 1989 A comparison among alternatives for using true potato seed technology Am Potato J66506
46 Kidane-Mariam H M H A Mendoza and R0 Wissar 1985 Performances of true potato seed families derived from inlermating tetraploid parental lines Am Potato J 62643-652
47 Rowell A B EEEwing and RLPlaisted 1986 Comparative field performance of potatoes from seedlings and tubers Am Potato J 63219-227
48 Elango F 1986 The role of high soil tempertures in the damping-off of trie potato seedlings in the lowland tropics Trop Agric 6366-67
49 Hoang V T P X Liem V B Dan N D Dam N X Linh N V Viet P X Tung and P ander Zaag 1988 True potato seed research and development in Vietnam Am Potato J65 295-300
50 Fernandez B B A STumapon LV Duna N M Balanay JP Kloos and PVander Zaag 1988 On-farm evaluation of true potato seed in the Philippines Am Potato J65 457-461
51 Li J H 1983 Prospects for the use of true seed to grow potato p 17-18 In W L Hooker (ed) Research for the potato in the year 2000 Intl Potato Ctr Lima Peru
52 Upadhya M D K C Thakur A juneja and M S Kadian 1985 True potato seed production flowering quality and economics p 117-147 In Report of a planning conference Innovative Methods for Propagating Potatoes held at Lima Peru Dec 10-14 1984
53 Turner A D and E EEving 1988 Effects of Photoperiod night temperature and irradiance on flower production in the potato Potato Res 31257-268
54 Rahimi F R and C D Carter 1989 Prevention of flower abscision after pollinatin in Solanum tuberosum cv Katahdin Am Potato J6647-51
55 Pallais N and N Espinola 1992 Seed quality as affected by nitrogen during true potato seed production and moisture conditions during storage Am Potato J6985-93
56 Pallais N H Asmat N Fong and JSantos-Rojas 1989 Factors affecting seedling vigor in potatoes I Stage of seed development Am Potato J66793-801
57 DAntonio V L and N A McHale 1988 Effect of storage temperature and extraction methods on dormancy and germination of true potato seed Am Potato J65573-581
58 Simmonds N W 1968 Prolonged storage of potato seeds Eur Potato J 11150-156
59 Barker W G and G R Johnston 1980 The longevity of seeds of the common potato Solanum tuberosum Am Potato J 57601-607
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
24
60 Howard H W 1969 The stoarge of true seeds of potatoes Eur Potato ) 12278-279
61 Howard H W 1980 Storage of true seeds of potatoes for 25 years Potato Res 23241-242
62 Simmonds N W 1962 Experiments on the germination of potato seeds I Eur Potato J 645shy61
63 Pallais N N Fong R Garcia and J Santos-Rojas 1990 Factors affecting seedling vigor in potatoes II Genotype dormancy and pre-sowing treatments Am Potato J 67109-119
64 Pallais N S Villagarcia N Fong J Tapia and R Garcia 1987 Effect of Supplemental nitrogen on true potato seed weight Am Potatoj 64 483-491
65 Dayal T R M D Upadhya and S N Chaturvedi 1984 Correlation studies on 1000-trueshyseed weight tuber yield and other morphological traits in potato (Solanum tuberosum L) Potato Res 27185-188
66 Bhagsari A S R EWebb S C Phatak and C A Jaworski 1988 Canopy photosynthesis stomatal conductance and yield of Solanurn tuberosum grown in a warm climate Am Potato J 65393-406
67 Zaag Van der P and A L Demagante 1987 Potato (Salanum spp) in an isohyperthermic environment I Agronomic management Field Crops Res 17 199-217
2f
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
25
Brief Resume of
Ajmer S Bhagsari Professor of Agronomy
School of Agriculture Home Economics and Allied Programs
Fort Valley State College
Formal Education
B S in Agriculture The Punjab University Chandigarh India 1954
M S in Agriculture The Punjab University Chandigarh India 1957
PhD Agronomy (Crop Physiology) The University of Georgia Athens Georgia 1974
Teaching Experience
Taught undergraduate courses in Soils and Soil Fertility Irrigation and Soil Conservation Crop Production Farm Management Experimental Designs Crop Improvement and Crop Physiology Origin and Classification of Soil 1958-1966
Taught undergraduate courses in Origin and Classification of Soils Crop Improvement Crop Physiology Plant Physiology Principles of Agronomy IPrinciples of Agronomy II1977-1992
Taught Crop Physiology to graduate students and supervised thesis research for three students
Research Experience
Photosynthesis and water stress studies with emphasis on peanut soybean seet potato potato and cassava Multiple cropping and symbiotic nitrogen fixation identification of physiological traits related to yield harvest index of root crops in relation to canop photosynthesis
a Identification of potato germplasm for the lowland tropics in Uganda effect of trellises and
grafted vs nongrafted rootstock on passion fruit production
e Served as principal investigator for the formula funded projects
Completed 1 Development of Antidotes for the Soybean Herbicide metribuzin
2 1890 Regional Project RR-3 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency
7(
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
26
3 Project No GEOX-5309 Improvement of Sweet Potato Yield and Quality through Characterization of Physiological Parameters
4 Physiological Processes for Sweet Potato Improvement
Active 1 1890 Regional Project RR-5 Improvement of Soybean for Stress Tolerance and Biological
Efficiency
Research Grants 1 1985-87 Screening grain legume germplasm for symbiotic nitrogen fixation and efficient
Rhizobuim strains (Funded for $100000) Dominican Republic
2 1988 Irish potato An alternate cash crop for Georgia farmers (Funded for two years for $40000)
3 1989 Black C C R H Brown and A S Bhagsari Characterization of Photosynthetic assimilation process in cassava and other Manihot species (Funded for $100000) CIAT Columbia South America
International Research and Experiment Station Visits
Dominican Republic from August 1983 to May 1987 Visited Dominican Republic in connection with research on nitrogen fixation in grain legumes and introduced germplasm for peanut soybean dry bean and sweet potato
Visited New Zealand Kiwi Research and Experiment Stations in New Zealdnd Nov 27 to Dec 6 1985
India (a)Visited Hort Dept Indian Agric Res Institute New Delhi (2) Banglore Horticultural Research Station Dec 1985 Germany and Holland Attended International Horticulture Congress in Germany and visited vegetable horticulture and Dyke farming in Holland and small vegetable farm in Germany August 1985
Uganda Attended First African Soil Science Society Congress Dec 5-10 1988
Uganda Sept 1989 - April 1992 Technical Advisor to Crop Science Department Makerere University Kampala Uganda Taught undergraduate and graduate students in agronomy and crop science Conducted research on potato and passion fruit in the lowland tropics provided evaluation for the release of two potato varieties
Service
Involved in service activities at the college level School of Agriculture Home Economics and Allied Programs Agricultural Research Station Served on numerous committees presented current research to visitors organized Sweet Potato Harvest Festival for many years Helping some farmers to enhance soybean marketing and export
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
27
0
Awards
Certificate of Excellence in Research and Grantsmanship FVSC May 1989
Research citation from NAFEO and USAID - 1988
R D Morrison and F E Evans Outstanding Scientist Award from the 1890 Assoc of Res Directors 1992
Publications
A REFEREED IOURNAL ARTICLES
1 Bhagsari A S and R H Brown 1976 Photosynthesis in Peanut (Arachis) Genotypes Peanut Sci 31-5
2 Bhagsari A S and R H Brown 1976 Translocation of Photosynthetically Assimilated C in Peanut (Arachis) Genotypes Peanut Sci 35-9
3 Bhagsari A S and R H Brown 1976 Relationship of Photosynthesis to Carbon Dioxide Concentration and Leaf Characteristics in Selected Peanut (Arachis) Genotypes Peanut Sci 3 10-14
4 Bhagsari A S and R H Brown 1976 Effect of Moisture Stress on Photosynthesis and Some Related Physiological Characteristics in Peanut Crop Sci 16712-715
5 Bhagsari A S D A Ashley R H Brown and H R Boerma 1977 Leaf Photosynthetic Characteristics of Determinate Soybean Cultivars Crop Sci 17929-932
6 Bhagsari A S 1981 Relation of Photosynthetic Rates to Yield in Sweet Potato Genotypes HortSci 16 779-780
7 Bhagsari A S and S A Harmon 1982 Photosynthesis and Photosynthate Partitioning in Sweet Potato Genotypes J Amer Soc Hort Sci 107 506-510
8 Mathur D D and A S Bhagsari 1983 Effect of Photosynthetically Active Radiation Temperature and Antitranspirants on Photosynthesis and Respiration of Leather Leaf Fern HortScience 18189-191
9 Bhagsari A S and R H Brown 1986 Leaf Photosynthesis and its Correlation with Leaf Area Crop Science 26127-132
10 Bhagsari A S 1988 Photosynthesis and Stomatal conductance of Selected Root Crops as Related to Leafage Crop Science 28902-906
11 Bhagsari A S R E Weble S C Phatak and C A Jaworski 1988 Canopy
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
28 Photosynthesis Stomatal Conductance and Yield of Solanum Tuberosum Grown in Warm-Climate Am potato j 65393-406
12 Bhagsari A S and D A Ashley 1990 The Relationship of Photosynthesis and Harvest Index to Sweet Potato Yield J Am Soc Hor Sci 115288-293
13 Bhagsari A S 1990 Photosynthetic evaluation of exotic sweet potato germplasm J Amer Soc Hor Sci 115634-639
14 Bhagsari A S 1993 Growth and Gas Exchange of Field-Grown Cassava in a Short-Season Environment Field Crops Res (under review)
B BOOK CHAPTERS
1 Phillips B A and A S Bhagsari 1978 Diazosulfonates as Protectants Against Airazine Toxicity to Soybeans p 21-34 In F M Pallos and J E Casida (Ed) Chemistry and Action of Herbicide Antidotes Academic Press Inc New York
2 Bhagsari A S 1989 Physiology of Yield In Jones A (Ed) Fifty years of Cooperative Sweet Potato Research 1939-1989 National Sweet Potato Collaborators Group
C PROCEEDINGS
1 Sikka L A SBhagsari JM Ssebuliba and R Kanzikwera 1991 Potato productionfrom true potato seed Proceedings Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa (In Press)
D BULLETIN ARTICLES
1 Bhagsari A S and B A Phillips 1978 Screening of Herbicidal Antidotes for Soybeans Re Bull Fort Valley State College Fort Valley GA Vol 11 p 15-19
2 Sapra et al 1984 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency Photosynthetic Activity and Translocation of Photosynthetic Soybean Genetics Newsletter IIpp 62-79
3 Sapra et al 1986 Evaluation of Soybean Germplasm for Stress Tolerance and Biological Efficiency (c) Harvest Index and Seed Yield Efficiency Soybean Genetics Newsletter 13 186-197
4 Bhardwaj H L and A S Bhagsari 1989 Harvest Index Yield and Physiological Characteristics of Soybean as Related to Seed Size Soybean Genetics Newsletter 16133-136
5 Bhardwaj H L and A S Bhagsari 1990 Harvest Index and Related Characteristics of Small and Large Seeded Soybean Genotypes Soybean Genetics Newsletter 17109shy113
(7) y-
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
29
E ABSTRACTS
1 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes Georgia Agron Abstr 1615
2 Bhagsari A S and RH Brown 1973 Photosynthesis in Peanut Genotypes JAmer Peanut Res and Educ Assoc 5 194
3 Bhagsari A S and R H Brown 1974 Photosynthesis and Respiration in Selected Peanut Genotypes Georgia Agron Abstr 173
4 Bhagsari A S and R H Brown 1974 Photosynthesis of Wild and Cultivated Peanuts S E Peanut Farmer 12(2) 14
5 Bhagsari A S D A Ashley H R Boerma and R H Brown 1975 Photosynthesis Photorespiration and Diffusive Resistance in Determinate Soybeans Agron Abstr p 68
6 Bhagsari AS D AAshley and H R Boerma 1976 The Effect of Leaf Age and Sink Demand on Certain Physiological Responses in Determinate Soybeans Agron Abstr p 69
7 Phillips B A and A S Bhagsari 1976 Herbicide Protectants for Soybeans 1 A Dexon-Triazine Interaction Bicentennial Meetings of the Historically Black Land-Grant Colleges and State Universities p 52
8 Ashley D A A S Bhagsari and H R Boerma 1976 Photosynthesis and C-Photosynthate Translocation in the Field-Grown Soybean Cultivars During Reproductive Development Agron Abstr p 69
9 Phillips BA and A S Bhagsari 1976 Evaluation of Some Sulfonic Acid Derivatives as Protectants Against Atrazine Toxicity to Soybeans Agron Abstr p 95
10 Bhagsari A S B A Phillips and B P Singh 1977 The Effect of Phenylmercuric Acetate on the Diffusive Resistance of Field Grown Soybeans Sou Assoc Agric Sci Abstr p 9
11 Singh B P B A Phillips and A S Bhagsari 1977 Nitrogenase Activity and Leaf Diffusion Resistance in Soybeans Effect of Soil Moisture Sou Assoc Agric Sci Abstr p1
12 Phillips B A and A S Bhagsari 1977 Diazosulfonates as Protectants Against Atrazine Toxicity on Soybeans Symposium on Chemistry and Action of Herbicide Antidotes 1973 Nat Amer Chem Soc Meeting Abstr PEST -066
13 Bhagsari A S 1978 Study Herbicidal Antidotes for Crop Protection Research Symposium (ARD) Historically Black Land-Grant Colleges and State Universities
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
30
14 Bhagsari A Sand B P Singh 1978 Diffusive Resistance Nitrogenase Activity and Yield of Leaf Hair Isogenic Soybeans under Moisture Stress Agron Abstr p 70
15 Bhagsari A S and BPSingh 1978 Effect of Antitranspirants on Diffusive Resistance and Yield of Soybeans Georgia Agron Abstr No 21 p 6
16 Bhagsari A S and B P Singh 1978 Effect of Moisture Stress on Diffusive Resistance Nitrogenanse Activity and Yield of Soybeans Sou Assoc Agric Sci Abstr p 5
17 Bhagsari A S 1979 Evaluation of Herbicidal Antidotes for Weed Control in Soybeans World Soybean Research Conference-Il p 109-110
18 Bhagsari A S 1980 Weed Control in Soybeans with Metribuzin Antidoted with Activated Charcoal Weed Sci Soc Abstr p 28
19 Bhagsari A S and S A Harmon 1980 Growth Analysis of 20 Sweet Potato Genotypes HortScience 15279
20 Bhagsari A S 1980 Photosynthesis of Sweet Potato Genotypes HortScience 15 279
21 Bhagsari A S 1980 Sweet Potato Dry Matter Accumulation Patterns third Biennial Research Symposium Historically Black Land-Grant Colleges and Universities p 28
22 Bhagsari A S and S A Harmon 1980 Screening Sweet Potato Genotypes for Photosynthesis Agron Abstr p 77-78
23 Bhagsari AS 1981 Study of Photosynthesis of Sweet Potato Genotypes HortScience 16 98
24 Bhagsari A S and S A Harmon 1981 Study of Morphological Traits of Sweet Potato Genotype -iScience 16288
25 Bhagsari A S and R K Abe 1981 Single Leaf Photosynthesis and its Correlations with Yield and Leaf Area Agron Abstr p 80
26 Bhagsari A S 1982 Biomass Production Potential and Photosynthetic Rate of Selected Sweet Potato Genotypes HortScience 17 p 156
27 Bhagsari A S and R K Abe 1982 Effect of Leaf Size on Phutosynthesis and Production of Sweet Potatoes HortScience 17 p 157
28 Bhagsari A S and R K Abe 1982 Photosynthesis 4C-translocation and Photosynthate Partitioning as Related to Sweet Potato Yield XX 1st International Horn Congress Hamburg Germany p 1452
29 Bhagsari A S and Arthur C Willis Jr 1982 Crop Growth and Storage Root
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
31
Development in Sweet Potato Genotypes 4th Biennial Research Symposium Historically Black Land-Grant Colleges and State Universities p 25
30 Mathur D D and A S Bhagsari 1982 Effect of Light Intensity Temperature and Antitranspirants on Photosynthesis and Respiration of Leather-leaf Fern Hort Sci Vol
17 (section 2) p 371
31 Bhagsari A S and Arthur C Willis Jr 1983 Canopy Photosynthesis and Dry Matter Partitioning of Selected Sweet Potato Genotypes HortScience 18 p 172
32 Bhagsari A S D A Ashley and Arthur C Willis Jr 1983 Relation of Photosynthesis and C-Assimilate Translocation to Yield of Selected Sweet Potato Genotypes Agron Abstr p 88
33 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops I Leaf Conductance and Photosynthesis HoriScience Vol 19 p 205
34 Bhagsari A S and Arthur C Willis 1984 Physiology of Root Crops II Canopy Photosynthesis and Dry Matter Partitioning HortScience Vol 19 Section 2 p 110
35 Bhagsari A S 1984 Photosynthetic Evaluation of Selected Soybean Genotypes and Their Parents World Soybean Research Conference Ill p 63
36 Bhagsari A S and Arthur C Willis Jr 1984 Photosynthetic Evaluation of Exotic Sweet Potato Germplasm ARD Symposium Dallas Texas p 28
37 Bhagsari A S A Essel J Polanco and J I Kirkwood 1984 Current Cropping Patterns and Future Prospects for Improvement in the Dominican Republic Agronomy Abstr p 53
38 Bhagsari A S 1985 Physiology of Root Crops III Effect of Leaf Age on Photosynthesis and Stomatal Conductance HortScience 20 p 655
39 Bhagsari A S 1985 Effect of Leaf Shape on Photosynthesis and Phytomass of Sweet Potato HortScience 20 p 581
40 Bhagsari A S 1985 Photosynthesis and Dry Matter Partitioning for Selected Cassava Genotypes Agronomy Abstr p 76
41 Bhagsari A S R EWebb C A jaworski and S C Phatak 1986 Photosynthesis and
Pholosynthate Partitioning of Solanum Tuberosum Grown in Warm Climate
HortScience 21 p 697
42 Bhagsari A S 1986 Comparative Photosynthetic Efficiency of Okra-Leaf versus Non-
Okra-Leaf Sweet Potato Genotypes HortScience 21 p 756
43 Yadava U L A S Bhagsari and R R Sharpe 1986 Influence of Phytohormone
Rootstock and Soil on Peach Leaf Gas Exchange Activities HortScience 21 p 895
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
32
44 Shoustari N H A S Bhagsari and M Latimore 1986 Response of Soybean Genotypes and Rhizobium Strains to different Soil PH Agronomy Abstr p 188
45 Bhagsari A S 1987 Effect of Plant Population on Photosynthesis and Dry Matter Partitioning of Sweet Potato HortScience 22 p 1113
46 Bhagsari A S and H LBhardwaj 1988 Comparative Growth and Yield of Selected Determinate Soybeans Abstracts of Technical papers SAAS Agronomy Division p 6
47 Bhardwaj H L and A S Bhagsari 1988 Physiological Characteristics of Selected Sweet Potato Genotypes as Affected by Age and Plant Density HortScience 23 p827
48 Bhardvaj H L and A SBhagsari 1988 Evaluation of Irish Potato as a Short-Season Crop HortScience 23 p 829
49 Bhardwaj H L and A S Bhagsari and D A Dillard 1988 Evaluation of an Exotic Rapeseed (Brasica compestris var toria) as a Short-Season Crop Agronomy abstr p 122
50 Bhagsari A S and H L Bhardwaj 1989 Relationship of Physiological Traits with Yield in Sweet Potato Cultivars HortScience Abstr p 88
51 Bhardwaj H L and A S Bhagsari 1989 Soybean harvest Index in Relation to Yield ARD-Eight Biennial Research Symposium October 7-1 1 1989
52 Bhagsari A S M Latimore D Rengifro L Tejeda and D A Ashley 1989 Evaluation of Grain Legume Germplasm for Symbiotic Nitrogen Fixation Agronomy Abstr p 50
53 Bhardwai H L and A S Bhagsarl 1990 Soybean harvest Index as Related to Yield Seed Size Plant Architecture and Economic Value Paper presented at Annual Meeting of American Society of Agronomy Oct 21-26 San Antonio Texas
54 Bhardwaj H L A S Bhagsari and K G Hayes 1990 Characters Associated with Yield Stability of Irish Potato Genotypes in Georgia HortScience 251173
55 Bhardwaj H L and A S Bhagsari 1991 Evaluation of Soybeans for Speciality Uses Annual Meeting of American Society of Agronomy Oct 27 - Nov 1 1991 Denver Colorado
56 Ssebuliba J M A S Bhagsari and C Nakawuka 1991 Variations in harvest Index of Cassava Genotypes Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
57 Bhagsari A S L Sikka JM Ssebuliba and D RAkimanzi 1991 Evaluation of Irish Potato Germplasm for Stress-Tolerance at Low Elevations in Uganda Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
33
Africa
58 Sikka L A SBhagsari JM Ssebuliba and RKanzikwera 1991 potato Production From True Potato Seed Ninth Symposium of the International Society for Tropical Root Crops Oct 20-26 Accra Ghana West Africa
59 Bhardwaj H Land A S Bhagsari 1991 Effect of Seed Tuber (source) on Potato Production in Georgia HoriScience 26775
60 Bhagsari A Sand 0 S Otim 1993 Effect of Trellis Types on Yield and Quality of Passion Fruit To be presented at the 90th meeting of the Amer Soc Hort Sci Nashville July 24-29 1993
61 Bhagsari A S and J M Ssebuliba 1993 Evaluation of Potato Germplasm in the Lowland Tropics To be presented at the 77th Annual Meeting of the Potato Assoc of Amer Aug 8-12 1993 Madison Wisconsin
q7
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
Kathleen G Haynes Research Plant Geneticist
Vegetable Laboratory
US Departnient of Agriculture
Agricultural Research Service
Rducational Backround
1978 BS in Biology LeMoyne College 1981 MS in Plant Breeding North Carolina State University 1985 Master of Statistics North Carolina State University 1986 PhD in Plant Brooding North Carolina State University
Research M rience
1978shy1980 Research Associate North Carolina State University
1987shy
present USDAARStVegetable Laboratory Beltsville Maryland
Professional Scientific Activities
Member American Genetic Association Member American Society for Horticultural Science
Member BLometrics Society
Member Crop Science Society of America Member Potato Association of America Member Gama Sigma Delta Member Sigma Xi Chair of the Breeding and Genetics Section of the PAA Member of the Finance Comittee of the PAA Secretary of NE 107
P1ubliains
1 Haynes KG and FL Haynes 1983 Stability of high specific gravity genotypes of potatris under high temperatures Am Potato J 6017-26
2 Haynes KG FL Haynes and WH Swallow 1987 Variability of flowering and 2n pollen production in diploid potatoes under high temperatures Am Potato J 6435-40
3 Haynes KG rL Haynes and WH Swallow 1988 Temperature and photoperiod effects on tuber production and specific gravity in diploid potatoes HortScience 23562-565
4 Haynes KG and FL Haynes 1988 The effect of day and night temperatures on bud initiation and flowering in diploid potatoes Am
Potato J 65589-596
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
35
1988 5 Owen HR RE Veilloux 7L Haynes and KG Haynes
Variability for critical photoperiod for tuberizatLon and tuber yield
J Amer monoploid anther-derved genotypes
of Solanum iphureia among Soc Hart Sci 113755-7S9
1988 Owen HR RE Veilleux FL Haynes
and RG Haynes6
Photoperiod effects on 2n pollen production response to anther culture
hureja Am and net photosynthesis of a diplandrous
clone of Solanum
Potato J 65131-139
FL Haynes and WR Henderson 1989 Heritability of 7 Haynes KG
specific gravity of diploid potato under high temperature growing
Crop Science 29622-625conditions
RE Webb RW Goth and DR Wilson 1989 The
8 Haynes EG correlation of yield and specific gravity
in the USDA potato breeding
Am Potato J 66587-592program
Goth RW JR Shumaker RE Webb DR Wilson R Moore LL Sanford
9 Nemarus A golden nematode resistant
russet-skin and KG Haynes 1989
Am Potato J 66703-707potato variety
Selection for tuber characters can199010 Haynes KG and FL paynes
maintain high specific gravity in a diploid potato breeding population
HortScience 25227-228
1990 Covariances between diploid parent and tetraploid
11 Haynes KG 3 offspring in tetraploid x diploid crosses
of Solanum tuberosum L
Heredity 81208-210
1991 Estimation of 12 Haynes KG WE Potts and MJ
Camp
preferential pairing in tetraploid x diploid hybridizations Theor Appl
Genet 81504-508
1991 Correlation of yield and specific 13 Haynes KG and DR Wilson
gravity in a tetraploid potato tuberling population Am Potato J
68355-362
EG Haynes and TH Barksdale 1991 Improvement of levels 14 Goth RW Plant Disease
of bacterial wilt resistanca in eggplant through breeding
75398-401
15 tialseth DE JB Sieczka DD Moyer RW Goth RE Webb DR
Wilson KG Haynes and WC Mehlenbacher 1991 Castile a multipleshy
purpose high-yieldLng full-season golden nematode resistant potato
Am Potato J 68453-459variety
Some aspects of inbreeding in derived tetraploids of
16 Haynes KG 1992 J Heredity 8367-70potatoes
Covariance between haploid-species hybrid and
17 Haynes XG 1992
Tuberosum x haploid-species hybrid in 4x-2x crosses of Lolanum tuberosu
L J Heredity 83119-122
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
36
Adipala Ekwamu Department of Crop Science
Faculty of Agriculture and Forestry Makerere University
P 0 Box 7062 Kampala UGANDA
Educational Background
1939-1965 Primary education at Kaberemaido and Kakure P7 Schools
1965 Obtained Grad A in Primary Leaving Examination
1966 Soroti College Obtained Junior Leaving Certificate Grade A
1967-1970 Secondary Education at Namilyango College
1970 Obtained East African Certificate of Education 5 Distinotions 3 Credits
1971-1972 Higher School education at Namilyango College
1972 Obtained the East African Advanced Certificate of Education 2 Principals 3 Subsidiaries
1973-1976 Undergraduate studies at Makerere University Kampala Uganda
1978 Awarded BSc (Agriculture) degree Upper Second Divison
1976-1980 Postgraduate studies Makerere University
Academic and Research Awards
1978 IDNO (Ormada) Fellowship for MSc studies at Makerere University
1985 FAO Fellowship to study seed radiation technology at the Atomic Energy Centre Viena Austria
1988 Commonwealth Fund for Technical Cooperation Fellowship to study intercropping at
The International Centre for Insect Physiology and Ecology (ICIPE) Nairobi
1988-1992 USAIDMFAD Fellowship for PhD study at The Ohio State University
1991 Rockefeller Foundation African Internship Fellowship for research on northern leaf bight
disease of maize in Uganda
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
37
Publications
1 Adipala E 1980 Disease of finger millet in Uganda MSc Thesis Makerere University 189
PP
2 Adipala E 1989 Host range Morphology and Pathogenecity of the genus Pyricularis in
Uganda E Afr Agric For J 54 (3)101-105
3 Adipala E 1990 The optimal spray regime for the control of important diseases of finger
millet in Uganda E Afr Agric For J55 (3)87-93
4 Adipala E 1992 Variation in field populations disease development and yield losses
associated with Exeerohilum turcicum in Uganda PhD Thesis The Ohio State University 158 pp
5 Adipala E 1993 Seedborne fungi of finger millet (Elausine coracana L Gartn) E Afr Agric
For J 58 (3) (in press)
6 Adipala E Lipps P E and Madden L V 1991 Reaction of corn lines from Uganda to
Exeerohilum turcicum (Abstr) Phytopathology 811146
7 Adipala E Lipps P E and Madden L V 1993 occurrence of Exeerohilum turcicum on
maize in Uganda Plant Dis 77202-205
8 Adipala E Lipps P E and Madden L V 1993 Reaction of maize lines from Uganda to
Exeerchilum turcicum Phytopathology 83217-223
9 Adipala E Taken J P Mukalere Z and Okoboi A 1993 The effect of intercropping maize
with finger millet on Physoderma maydis and exserohilum turcicum infections E Afr Agric
For J68 (3) (in press)
10 Adipala E and Mugerwa J S 1992 Effect of nitrogen phosphorus and potassium
fertilization on the yield and chemical composition and Amaranthus hybridus subspecies hybridus (Greenhead) E Afr Agric For J (in press)
11 Adipala E and Mukiibi J K 1992 Determination of the optimal time to start and to stop
spraying against important fungal diseases of finger millet in Uganda E Afr Agric For J (in press)
12 Ekwamu A 1991 Influence of head blast infection on seed germination and yield
components of finger millet (Eleusine coracana LGaertn) Tropical Pest Management 37122shy123
13 Ekwamu A Lipps P E and Pratt R V 1991 Reaction of 150 OHS10 S lines to
Exeerohilum turcicum race 1 Agronomy Abstr Annu Meeting 1991 pp 92
14 Elobu P and Adipala E 1993 Prevalence of finger millet diseases in Kaberamaido
subcounty Uganda Uganda J Agric Sciences (in press)
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
38
Clement Rogers Kanzikwera Scientific OfficerAgronomist Kalengyere Research Station
Educational Background
Bachelor of Science in Agriculture (Honours) - Lower Division-Makerere University 1976
Concurrent diploma in Education-Makerere University 1976
Appointments
Graduate Teacher 1 July 1976
Agricultural Officer 1 July 1979
Scientific OfficerAgronomist 1 July 1990
Positions Held
Head of Agriculture Department-Nyakasura School (1977-1979)
Agricultural OfficerExtension and Deputy District Agricultlural Officer-Rukungiri (1980-1983i
Agriculutral OfficerPotato Extension-Kabale District (1983-1985)
Agricultural Officer in charge of Kisoro sub-District and potato extension (1986-1989)
Scientific OfficerAgronomist Kalengyere Research Station (1990-1993)
Main Duties Executed
1 Extension supervision and training 2 Management of potato variety trial centres 3 Potato germplasm management 4 Potato variety development 5 Agronomic research on potato 6 Deputy Director Kalengyere Research Station 7 Head Potato Programme Kalengyere Research Station 8 Compilation of research reports
Courses and Conferences Attended
1 The 18th International Potato course on potato production and post harvest technology April-July 1989 Wageningen The Netherlands
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993
39
2 Workshop on the production post-harvest technology and utilization of potato in the warm tropics 23-27 July 1990 Redult Mauritius
3 Virology and Tissue Culture Course 6-9 August 1990 Ruhengeri Rwanda
4 1st National Scientific Conference December 1990 Kampale Uganda
5 Seed multiplication course through farmers-International Potato Centre Region III 18-23 June 1990 Kabale Uganda
6 Course on diseases of potato and sweet potato-MOA MFAD USAID CIP-Region III 19-22 November 1990 Kabale Uganda
7 Potato production course (Resource person) 7-11 September 1992 Mityana Uganda
Publication and Papers Presented
1 Kanzikwera R C 1975 Nutrient requirements of Carnations (Dianthus caryophillus)
2 Kanzikwera R C 1989 Potato production in Uganda-Presented at the International Agricultural Centre Wageningen The Netherlands June 1989
3 Sikka L C and Kanzikwera R C 1990 Recent developments in potato improvement in Uganda
4 Sikka L C and Kanzikwera R C 1990 Evaluation of solanum potato genotypes for wider adaptability and resistance to late blight inUganda
5 Kanzikwera R C at al 1990 True Potato Seed development in Uganda
Current Status
Member of the African Potato Association Undertaking MSc Programme ending June 1993