Agilent Technologies Prep LC Columns Technologies Prep LC Columns ZORBAX CombiHT Preparative Columns • For rapid preparative separations and fast re-equilibration in the 15-50 mg

Agilent Technologies Prep LC Columns

Agilent Technologies Prep LC Columns

ZORBAX CombiHT Preparative Columns• For rapid preparative separations and fast re-equilibration in the 15-50 mg range • Finger tight connections supply high pressure seal up to 7,000 psi • End-fittings are reusable - only the column barrel itself is replaced• Packed with ZORBAX StableBond (for low pH) and Eclipse XDB (for mid-range pH)

for maximum column life• Other column packing types can be supplied upon request• For combinatorial chemistry, medicinal chemistry, or other high-throughput applications• Complementary scale up from rapid analytical separations• For fast gradient or fast isocratic separations

Agilent Technologies has always taken seriously its responsi-

bility to ensure your success. That’s why all our instruments

and supplies are manufactured with strenuous quality control

practices and continuous improvement programs. Under that

philosophy, Agilent Technologies has developed an entire

family of preparative and semi-preparative columns, all part

of the ZORBAX line. Recognized throughout the industry,

the ZORBAX name is not only synonymous for superior

performance, reliability and consistency but it’s also seen

as the perfect fit for Agilent instrumentation. Built on the

tradition of the 1100 HPLC platform, it’s safe to say ZORBAX

gives the life science LC leader a complete system.

Zorbax RP-HPLC Column Chemistry DescriptionsZorbax RP-HPLC preparative columns are offered in a variety of bonded phase types, depending on the pH of the mobile phase. In general, StableBond columns are recommended for low pH, Eclipse XDB and Bonus RP columns for mid-range pH, and Extend-C18 for high pH separations. Listed on the following pages are thecharacteristics of each bonded phase.

ZORBAX StableBond (80 Å) Column Ordering Information*

Description Size (mm) Particle SB-C18 SB-C8 SB-CN SB-C3 SB-PhenylSize (µm) USP L1 USP L7 USP L10 (80 Å) USP L11

(80 Å) (80 Å) (80 Å) (80 Å)

Standard Columns and Bulk Packing

Preparative 21.2 x 250 7.0 880975-102 880967-106 880975-105 880975-112

Semi-Preparative 9.4 x 250 5.0 880975-202 880967-201 880975-205 880975-209 880975-212

Semi-Preparative 9.4 x 150 5.0 883975-902



Guard Cartridge 9.4 x 15 7.0 820675-115 820675-115 820675-124 820675-115(semiprep)

Semiprep Guard Column Hardware Kit (9.4 mm ID) 840140-901including: inlet fitting, end fitting, column connector

CombiHT High Throughput Preparative Columns

CombiHT 21.2 x 150 5.0 870150-902 870150-906

CombiHT 21.2.x 100 5.0 870100-902 870100-906

CombiHT 21.2 x 50 5.0 870050-902 870050-906

CombiHT End Fittings (required for use) 820400-901

CombiHT Replacement Seal 820385-901

* All configurations not shown are available by special products quote.

ZORBAX StableBond (300 Å) Column Ordering Information*

ZORBAX StableBondColumns (80 Å and 300 Å)• Longer column lifetime, reproducibility

for low pH separations of basic and other compounds.

• Unique monofunctional silanes with bulky diisobutyl (with C18) or diisopropyl (with C8, C3, Phenyl, propyl CN) side chain groups sterically protect the key siloxane bond from hydrolytic attack at low pH. To maximize reproducibility under acidic pH conditions, StableBond packing materials are not endcapped.

• Broad selectivity using a wide selection of stable short-chain phases

• Use temperature as a retentivity/ selectivity variable for such difficult compounds as hydrophobic peptides

• Excellent for beginning method development for small molecules

• Excellent for peptide/protein separations

• No bleed for LC/MS applications

Reversed Phase

ZORBAX Eclipse XDB Column Ordering Information*

Description Size (mm) Particle 300SB-C18 300SB-C8 300SB-CN 300SB-C3Size (µm) USP L1 USP L7 USP L10 (300 Å)

(300 Å) (300 Å) (300 Å)

Standard Columns

Preparative 21.2 x 250 7.0 880995-102 880995-106 880995-109 880995-105

Semi-Preparative 9.4 x 250 5.0 880995-202 880995-206 880995-209 880995-205

Guard Cartridges, 2/pk 9.4 x 15 7.0 820675-124



Description Size (mm) Particle XDB-C18 XDB-C8 XDB-Phenyl Size (µm) USP L1 USP L7 USP L11

(80 Å) (80 Å) (80 Å)

Standard Columns and Bulk Packing

Semi-Preparative 9.4 x 250 5.0 990967-202 990967-206 990967-212

CombiHT Columns

CombiHT 21.2 x 150 5.0 970150-902 970150-906

CombiHT 21.2 x 100 5.0 970100-902 970100-906

CombiHT 21.2 x 50 5.0 970050-902 970050-906

CombiHT End Fittings (required for use) 820400-901

CombiHT Replacement Seal 820385-901


ZORBAX Eclipse XDB• Provides good peak shape for basic,

acidic and neutral compounds at mid-range pH

• Long column life at mid-range pH insured by unique chemistry• Dimethylalkyl phase• Proprietary double endcapping • Use of Zorbax Rx-SIL with uniform

wall thickness• Complementary selectivity to

StableBond, Bonus-RP or Extend columns

• Recommended maximum aqueous concentration 100% for XDB-Phenyl, 95% for XDB-C18, 90% for XDB C8

Reversed Phase

ZORBAX Extend-C18 Column Ordering Information

• Please use part number 899999-999, and request a SPQ (special product quotation). Include in your request the column dimension (e.g., 21.2 x 50 mm), bonded phase (e.g., Extend-C18), pore size (e.g., 80 Å), and particle size (e.g., 5-µm)

ZORBAX Bonus-RP• Embedded polar group chemistry (amide) provides good peak shape at both low

and mid-range pH• Unique bulky diisopropyl groups sterically protect the bonded phase from hydrolysis and

loss, which provides maximum lifetime at low pH• Useable in 100% aqueous mobile phases• Unique selectivity complementing alkyl phases such as C18 or C8• Unique triple endcapping deactivates silanols• ZORBAX Rx-SIL sol-based ultrapure silica (99.995%) that ensures long column life at mid-range pH

ZORBAX Extend-C18• Good peak shape and long column life at high pH for separation of basic, acidic and neutral compounds • Extends column utility to a variety of applications:

• Separating basic compounds above their pKa in free base (uncharged) form giving added retention and/or alternate selectivity• Using ammonium hydroxide as mobile phase with LC/MS with peptides or small molecules for excellent signal/noise

performance and alternate selectivity • General separation of basic, acidic, neutral compounds at high pH providing different selectivity or improved solubility

• Uses a novel C18/C18 bidentate structure and is also double endcapped using a proprietary procedure for increased stability at high pH

• Built on highly purified ZORBAX Rx-SIL, a sol-based silica that contributes to improved column life at high pH• Complete useable range from pH 2-11.5 and may be appropriate for use in low and intermediate pH mobile phases

ZORBAX Bonus-RP Column Ordering Information

• Please use part number 899999-999, and request a SPQ (special product quotation). Include in your request the column dimension (e.g., 21.2 x 50 mm), bonded phase (e.g., Bonus-RP), pore size (e.g., 80 Å), and particle size (e.g., 5-µm)

ZORBAX Original Column Ordering Information*

Description Size (mm) Particle ODS C8 Phenyl CN TMSSize (µm) (C18) (70 Å) (70 Å) (70 Å) (70 Å)

(70 Å)

Standard Columns

Preparative 21.2 x 250 7.0 880952-102 880952-106 880952-105

Semi-Preparative 9.4 x 250 5.0 880952-202 880952-201 880952-212 880952-205 880952-210

Guard Cartridges 9.4 x 15 7.0 820675-115 820675-124



ZORBAX Original Columns• High surface area (300 m2/gm, 20%

carbon load for C18) gives added retention

• Uses the Original Zorbax SIL support,monolayer silane bonding, and singleendcapping for deactivation of residual silanol groups

ZORBAX Rx Reversed-Phase HPLC Columns• Offers high stability and good peak

shape for low pH applications• Rx-C18 manufactured using

dimethyloctadecylsilane, non-endcapped

• Recommended for alternate selectivityat low pH to StableBond; for higher temperature applications, StableBondis recommended

ZORBAX Rx Column Ordering Information*

Normal Phase

ZORBAX Rx Silica

• Made from highly pure (>99.995%) porous silica microspheres (pore size is the space between the solid silica microparticles)

• Stronger than other silica types• Low acidity and low metal content

make ZORBAX Rx-SIL ideal for normal-phase separation of polar compounds that exhibit poor peak symmetry on more acidic silica

• Useful for very hydrophilic compoundswith high organic mobile phases

ZORBAX CN

• Cyanpropyldimethylsilane monolayer bonded to ZORBAX SIL

• Equilibrates more rapidly than ZORBAX SIL, and is used for many ofthe same normal phase applications

• Less prone to fouling than silica

ZORBAX Normal Phase Column Ordering Information*

ZORBAX Normal Phase HPLC ColumnsFor normal phase chromatography, the ZORBAX product line offers a choice of bonded and non-bonded silica packings.

ZORBAX NH2

• Propyl-amino silane phase bonded to ZORBAX SIL• Used for normal phase and weak anion-exchange,

and reversed phase HPLC of polar compounds• Vitamins A and D are separated in the normal

phase mode and carbohydrates and sugars are separated in the reversed phase mode on ZORBAX NH2

Description Size (mm) Particle RX-C18 RX-C8 Size (µm) (80 Å) (80 Å)

Standard Columns

Preparative 21.2 x 250 7.0 880967-102 880967-106

Semi-Preparative 9.4 x 250 5.0 880967-202 880967-201

Guard Cartridge packed 9.4 x 15 7.0 820675-115with SB-C8


* All configurations not shown are available by special products quote.Note - Rx-C8 is same the product as StableBond C8.

Description Size (mm) Particle Rx-SIL SIL CN NH2

Size (µm) (80 Å) (70 Å) (70 Å) (70 Å)

Standard Columns

Preparative 21.2 x 250 7.0 880975-101 880952-101 880952-105 880952-108

Semi-Preparative 9.4 x 250 5.0 880975-201 880952-201 880952-205 880952-208

Guard Cartridges 9.4 x 15 7.0 820675-119 820675-111



Reversed Phase

ZORBAX Ion Exchange Column Ordering Information*

Ion Exchange

Description Size (mm) Particle Reusable SAX SAX SCXSize (µm) Cartridge (70 Å) (300 Å) (300 Å)

Hardware

Standard Columns

Semi-Preparative 9.4 x 250 5.0 880952-203 880952-204

Guard Cartridges, 4/pk

Reusable Cartridge Hardware


ZORBAX Ion ExchangeColumns• Zorbax SAX is manufactured with

70 Å Zorbax SIL porous silica microspheres with a trifunctional quaternary amine group

• Zorbax SCX is manufactured with 300 Å Zorbax SIL porous silica microspheres with an aromatic sulfuric acid group

• Provides rapid separations (pressure limit 5,000 psi)

• High efficiency, rapid separations

Gel Filtration

ZORBAX GF-250 and GF-450 Gel FiltrationColumns• Rapid separations and extended

column lifetime – often twice that of other size exclusion columns

• High recovery for most proteins (>90%)

• Usable with organic modifiers and denaturants such as 8M urea, 6M guanidine hydrochloride, and SDS

• Silica surface stabilized with zirconia,then coated with a monolayer diol phase providing a broad pH range of 3-8.5

• Zorbax SIL particles provide a silica support with high physical stability

Description Size (mm) GF-250 GF-450 (4.0 µm, 150 Å) (6.0 µm, 300 Å)

Standard Columns

Preparative 21.2 x 250 884974-901 884974-910

Semi-Preparative 9.4 x 250 884973-901 884973-902

Guard Cartridge, 2/pk 9.4 x 15 820675-111

Hardware Kit 9.4 x 15 840140-901


ZORBAX Normal Phase Column Ordering Information*

Zorbax Bio Series OligoColumns• Mixed mode ion exchange and

reversed phase columns specifically developed for oligonucleotide purification

• Unique ligand is bonded to 5 µm, 150 Å silica that has been stabilized with zirconium-oxide. Denaturants can be used to prevent self-hybridization

ZORBAX Oligo Column Ordering Information*

Other Vendors

Vydac Reverse PhaseSemi-PreparativeColumns • Vydac C4 (214TP) reverse phase

packings consist of butyl aliphatic groups bonded to high-puritysilica with 300 Å diameter pores

• Vydac C18 (218TP) reverse phase packings consist of octadecyl aliphatic groups bonded to high purity silica with 300 Å diameter pores

• The alkyl silanes used to bond both of these phases to the silica are polyfunctional resulting in a cross-linked or polymeric hydrophobic phase –this provides long column lifetime and no measurable phase leaching

• These are very popular phases for the reverse phase separation of polypeptides

Vydac Column Ordering Information

Description Size (mm) Particle Size (µm) Oligo (125 Å)

Standard Columns


Preparative 21.2 x 250 5.0 884974-915


Description Size (mm) Particle Reverse Phase Size (µm) C4 (214 TP) C18 (218 TP)

(300 Å) (300 Å)Standard Columns

Semi-Preparative 10.0 x 250 5.0 79918B3-588 79918O3-588

Oligonucleotide Separation

To order or for technical assistance,call Agilent Technologies at 800-227-9770, or contact your localauthorized Agilent distributor.

© Agilent Technologies, Inc. 2001

Information, descriptions and specifications in this publicationare subject to change without notice.

All rights reserved. Reproduction, adaptation, or translation without prior written permission is prohibited, except as allowed under copyright laws.

Printed in the U.S.A. August 31, 20015988-2103 EN

Documents

Agilent Technologies Prep LC Columns Technologies Prep LC Columns ZORBAX CombiHT Preparative Columns • For rapid preparative separations and fast re-equilibration in the 15-50 mg