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Page 1Page 1
Agilent’s New 7000B Quadrupole GC/MS/MS:
Evolving technology for higher performance tools
Terry L. Sheehan, Ph.D.GC/MS/MS Product ManagerAgilent TechnologiesSanta Clara, CA
Page 2
Triple Quadrupole MS/MS(also called tandem quadrupole)
is changing the way scientiststhink about GC/MS
Page 3
Transmission Quadrupole MS & MS/MSSensitivity and Selectivity Scale
Least Sensitive Most Sensitive
Fast 12,500 u/s (5975C)Fast 6,250 u/s (7000)
Typical < 2,000 u/s
Targets & Non-targetsTargets & Non-targets
*Multiple Reaction Monitoring Also called SRM for Selected Reaction Monitoring (similar to SIM – Selected Ion Monitoring)
Dwell 20-50 ms
Targets onlyDwell 1-50 ms
< <
Page 4
Transmission Quadrupole MS & MS/MS Sensitivity and Selectivity Scale
Least Sensitive Most Sensitive
Fast 12,500 u/s (5975C)Fast 6,250 u/s (7000)
Typical < 2,000 u/sDwell 20-50 ms
Least Selective Most Selective
Unit mass resolutionUnit mass + AMDIS
deconvolution
Targets & Non-targetsTargets & Non-targets
Targets only
Targets only per DBL
Dwell 1-50 ms
< <
< <
Page 5
Summary of Relative Performance Factors
MDL = Sanalyte
Nneutral + Nmatrix + NGC “bleed”
Quant Error = Smatrix
Sanalyte Smatrix+
GC/MSD Scan
GC/MSD SIM
GC/MSD Scan-DRS
GC/TQ MS/MS
S from analyte + +++ + +++S from matrix -- -- - 0N from neutrals = = = Ultra lowN from matrix --- --- - 0N from "bleed" Low Low Lower 0MDL (clean) Very Low Lowest Lower LowestMDL (very dirty) Low Low Lower LowestQuant Error Low Low Lower Lowest
But scan is essential for non-targets (unknowns)!
Page 6
Fundamental Benefits of MS/MS for GC
Consistently High Sensitivity for Target Analytes in Complex Matrices
Higher Selectivity to Identify Target Analytes in Complex Matrices
Page 7Page 7
MS/MS Eliminates Scan and SIM Interferences
Single Quad MSselectivity proportional to
spectral resolutionno selectivity against ions
with same m/zinterference
analyte
unit mass resolution
Page 8Page 8
MS/MS Eliminates Scan and SIM Interferences
analyte
Product 1Product 3
Product 2
Single Quad MSselectivity proportional to
spectral resolutionno selectivity against ions
with same m/zinterference
analyte
unit mass resolution
Product Ion Spectrum
Page 9Page 9
MS/MS Eliminates Scan and SIM Interferences
analyte
Product 1Product 3
Product 2
Single Quad MSselectivity proportional to
spectral resolutionno selectivity against ions
with same m/z
Triple Quad MSHigh probability that one or more of the
product ions will be a unique dissociation product of the precursor only
interference
analyte
interference
unit mass resolution
Product Ion Spectrum
Page 10Page 10
MS/MS Ensures Lowest Detection LimitsEI: spectrum of analyte can also include ions from matrix, column bleed, gases, etc.
Q1 SIM isolate precursor before CID
chemical noise from these other ions is eliminated
Page 11Page 11
MS/MS Ensures Lowest Detection LimitsEI: spectrum of analyte can also include ions from matrix, column bleed, gases, etc.
Q1 SIM isolate precursor before CID
Product 1Product 3
Product 2
CID +Q2 SIM
Lower m/z Product Ions measured against zero chemical noise for consistently lower LOQ and LODs
chemical noise from these other ions is eliminated
Page 12Page 12
SIM Limited by Unit Mass Resolution
GC/MS Single Quad SIM
Interfering matrix peaks = chemical noise
(mainlib) Eicosane20 40 60 80 100 120 140 160 180 200 220 240 260 280
0
50
100
29
43
57
71
85
99113 127 141 155 169 183 197 211 225 238 253 282
(mainlib) Benzene, hexachloro-20 40 60 80 100 120 140 160 180 200 220 240 260 280 300
0
50
100
2435
47
53
71
8395
107
118
130
142
153 165
177
190
214
237
249
284
Cl
Cl
Cl
Cl
Cl
Cl
in diesel fuel
C20 Eicosane Hexachlorobenzene
Page 13Page 13
SIM Limited by Unit Mass Resolution
GC/MS Single Quad SIM
Interfering matrix peaks = chemical noise
(mainlib) Eicosane20 40 60 80 100 120 140 160 180 200 220 240 260 280
0
50
100
29
43
57
71
85
99113 127 141 155 169 183 197 211 225 238 253 282
(mainlib) Benzene, hexachloro-20 40 60 80 100 120 140 160 180 200 220 240 260 280 300
0
50
100
2435
47
53
71
8395
107
118
130
142
153 165
177
190
214
237
249
284
Cl
Cl
Cl
Cl
Cl
Cl
in diesel fuel
Page 14Page 14
SIM Limited by Unit Mass Resolution
GC/MS Single Quad SIM
Interfering matrix peaks = chemical noise
At unit mass resolution SIM, HCB is buried by the more intense peaks from C20 to C22 hydrocarbons
in diesel fuel
Page 15Page 15
MS/MS Succeeds Where MS Fails
GC/MS Triple Quad SRM
GC/MS Single Quad SIM
A chromatographer’s dream: single peak on flat baseline
Interfering matrix peaks = chemical noise
in diesel fuel
Page 16
Pesticide Analysis: Comparing SIM to MS/MS
Apple
Orange
Spinach
Ginseng
Cabbage
SIM ‐ EIC (246)
XX
Page 17
Pesticide Analysis: Comparing SIM to MS/MS
Apple
Orange
Spinach
Ginseng
Cabbage
SIM ‐ EIC (246) MRM (2 transitions)
A c q u is it io n T im e (m in )1 1 .8 1 1 .9 1 2 1 2 .1 1 2 .2 1 2 .3
Cou
nts 4x1 0
-0 .1
0
0 .1
0 .2
0 .3
0 .4
0 .5
0 .6
0 .7
0 .8
0 .9
1
1 .1
2 4 6 .0 -> 1 7 6 .1 , 2 4 6 .0 -> 1 7 5 .1
R a t io = 2 3 .5
A c q u isitio n T im e (m in )1 1 .8 1 1 .9 1 2 1 2 .1 1 2 .2 1 2 .3
Cou
nts 3x1 0
0
1
2
3
4
5
6
7
8
2 4 6 .0 -> 1 7 6 .1 , 2 4 6 .0 -> 1 7 5 .1
R a t io = 2 3 .6
A c q u is it io n T im e (m in )1 1 .8 1 1 .9 1 2 1 2 .1 1 2 .2 1 2 .3
Cou
nts 4x 1 0
-0 .1
0
0 .1
0 .2
0 .3
0 .4
0 .5
0 .6
0 .7
0 .8
0 .9
1
1 .1
1 .2
2 4 6 .0 -> 1 7 6 .1 , 2 4 6 .0 -> 1 7 5 .1
R a t io = 2 4 .0
A c q u is it io n T im e (m in )1 1 .8 1 1 .9 1 2 1 2 .1 1 2 .2 1 2 .3
Cou
nts 3x1 0
0
1
2
3
4
5
6
7
8
2 4 6 .0 -> 1 7 6 .1 , 2 4 6 .0 -> 1 7 5 .1
R a t io = 2 2 .9
Acquisition Time (min)11.8 11.9 12 12.1 12.2 12.3
Cou
nts 4x10
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
246.0 -> 176.1 , 246.0 -> 175.1
Ratio=23.3
S/N = 448
S/N = 456
S/N = 260
S/N = 446
S/N = 241
XX
Page 18
7000B MS/MS: High Performance Components
New inert, high temp, extraction EI source
New Ramped Iris Triple-Axis Detector
Gold plated quartz, hyperbolic quadrupoles
Split Flow Turbo
High pressure hexapole collision cell with Patent Pending Helium
Quench Gas
Hinged access to source and detector
The best technologies from the industry leading 5975C Series GC/MSD
– Heated monolithic gold plated quartz quadrupole
– Reliable, inert, high performance ion source
– High Signal/Low Noise Triple-Axis Detector
– PCI/NCI ion source
…and the 6400 Series LC/MS/MS
– Linear acceleration enhanced hexapole collision cell
– MassHunter software
Page 19
7000B MS/MS: Hot (200ºC) Quartz Analyzers
Gold plated quartz, hyperbolic quadrupoles
High temperature analyzers stay clean even with highly concentrated, complex matrices
Page 20
Proprietary Heated Quartz Quadrupole
• High temperature (200ºC)operation results in greater reliability with “dirty” samples
– MS/MS methods typically have more “dirty” samples and benefit even more from the higher temp quad analyzer
– Virtually eliminates the need to clean quads as a maintenance issue
• More stable tunes and methodsover a longer period of time in real world sample environments
Page 21
Hyperbolic, Gold-coated, Quartz - Proprietary!
• Why quartz?– Low coefficient of linear expansion (expansivity; α)
• dL = α L0 dt [t = ºC; dL = change in length (m); L0 = length at 0 ºC]
– α varies greatly for different materials (m/m K x 10-6)• Stainless steel 304 = 17.3; Stainless steel 316 = 16.0• Inconel (Ni alloy) = 12.6; Monel (Ni/Cu alloy) = 13.5• Glass pyrex = 4.0; Quartz, fused = 0.59
– For change from 25 to 150 ºC, which material expands the LEAST?
• But what is the “analytical value?”• Low thermal expansion means dimensions/alignments do not change• After maintenance, the quartz quad can be baked at 200 ºC • Under operation, the quartz quad can be run at 150, 180 or even 200 ºC• Heat is the “friend” of GC/MS methods – true for the source and analyzer!
Page 22
More Information on the Agilent Website
Detailed Video
Page 23
7000B MS/MS: Collision Cell with Helium Quench
High pressure hexapole collision cell with Patent Pending Helium
Quench Gas
Highly efficient dissociation of precursor ions
Maximum transitions per second without product ion carryover (cross-talk)
Reduction of metastable helium from EI source
Page 24
Hexapole Cell: Better Transmission Characteristics
00.20.40.60.8
11.2
0 500 1000 1500
Transmission
m/z
Mass Range TransmissionQuadrupole Hexapole Octapole
GC/MSm/z=1050
Quadrupoles are the best mass filters (analyzers)Hexapoles and octapole are the best transmission devices
Page 25
500 Transitions/sec: Why Is This Important?
• Narrow GC peaks (width <1-2 s)• Sufficient data points (15) to perform acceptable/accurate
quantification• Many compounds to monitor in single run (multiresidue)
when coeluting peaks are quite frequent• Regulated environment where QC checks are required to
ensure data validity– Confirmatory transitions according to the 96/23/CE directive
Page 26
Measured Collision Cell Clearing Time
1 msec
No product ion signal = no cross-talk at 500 transitions per second
Page 27
Collision Cell Process: Typical Description
Collision Cell
Precursor Ions In
Product Ions Outcollision induced dissociation
1 ml/min N2Collision Gas
Source Detector
Quad Analyzer Quad Analyzer
This is a good description for LC/MS/MS, but it is not complete for GC/MS/MS
Page 28
Collision Cell Process: Full Description for GC/MS
Collision Cell
Precursor Ions InHe* +
Product Ions Out + He*
In GC/MS, neutral noise is buried in much higher chemical noise.
In GC/MS/MS, chemical noise is greatly reduced so neutral noise is a critical source of noise.
collision induced dissociation
A high population of highly energetic helium metastables are produced in an EI source; since metastable helium is not charged, it can pass through mass analyzer field into the collision cell and through to the HED-EM
1 ml/min N2Collision Gas
At the detector, metastable helium generates neutral noise.
Source Detector
Quad Analyzer Quad Analyzer
Page 29
Collision Cell Process with Helium Quench Gas
Collision Cell
Precursor Ions InHe* +
collision induced dissociation
1 ml/min N2Collision Gas
Transmission of metastable helium to the detector is greatly reduced; the Triple-Axis detector further reduces neutral noise for ultra-low neutral noise.
Source Detector
Quad Analyzer Quad Analyzer
He Buffer Gas
He* + He → 2 He + heat
Product Ions Out
+ He*
Page 30
Benefit from “Helium Quench”
3x10
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
5.5
6
6.5
7
7.5
8
+ MRM (283.79999 -> 213.90039) 1pgHCB_std_cond_UnkGain_2090EMV_He20pct_04.d Noise (RMS) = 6.03; SNR (6.465min) = 1294.0
6.465
Counts vs. Acquisition Time (min)4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 5 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9 6 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 7
3x10
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
5.5
6
6.5
7
7.5
+ MRM (283.79999 -> 213.90039) 1pgHCB_std_cond_UnkGain_2090EMV_He0pct_05.d Noise (RMS) = 20.08; SNR (6.470min) = 341.0
6.470
4.628 6.301
Counts vs. Acquisition Time (min)4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 5 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9 6 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9
Helium Quench Gas ON
Helium Quench Gas OFF
tested with hexachlorobenzeneSame
Signal
SameSignal
S/N 341:1
S/N 1294:1
Noise Decreased2x10
0.5
0.6
0.7
0.8
0.9
1
1.1
1.2
1.3
1.4
1.5
1.6
1.7
1.8
1.9
2
+ MRM (283.79999 -> 213.90039) 1pgHCB_std_cond_UnkGain_2090EMV_He0pct_05.d Noise (RMS) = 20.08; SNR (6.470min) = 341.0
Counts vs. Acquisition Time (min)4.7 4.75 4.8 4.85 4.9 4.95 5 5.05 5.1 5.15 5.2 5.25 5.3 5.35 5.4 5.45 5.5 5.55 5.6 5.65 5.7 5.75 5.8 5.85 5.9 5.95 6 6.05 6.1 6.15 6.2
In MS apps, neutral noise is not visible – it is buried by more intense chemical noise.Since MS/MS eliminates chemical noise, neutral noise is visible and mustbe minimized.
Page 31
7000B MS/MS: New Higher Sensitivity Source
New inert, high temp, extraction EI source
Increase signal for precursor ions using an extractor source design
Page 32
Lab-Proven Inert Source
• Solid, inert materials, not a coating that can be damaged by cleaning
• Stay-Clean (350ºC) designextends maintenance intervals with dirty samples
• Source tune parameters in the Autotune file
• Dual filaments reduces maintenance intervals
• 1000’s of satisfied MSD usersPartition between source and analyzer compartments support dual stage pumping
Page 33
7000B Extraction SourceExternal View - Assembled
At first look, it does not look any different than the 7000A source
Like the 7000A source, the 7000B extraction source is more open than the MSD source – looks similar to the CI source
Page 34
7000B Extraction SourceExpanded View of Components
And there is new connection to the lens #11
Component #7 is a new insulator
Design uses combination of “push” (repeller) and “pull” (extractor lens) to increase ion intensity
Page 35
7000B Increased Signal
Extractor Source w/Ramped Iris Detector
Standard Source & Detector mode
Extractor source cutaway
Push-Pull Principle:Repeller and Extractor
Page 36
Why Is the New Extractor Source More Valuable for
Triple Quad MS/MS than
Single Quad MSD?
Page 37Page 37
Extractor Source Increases Signal for All IonsEI: spectrum of analyte can also include ions from matrix, column bleed, gases, etc.
Single Quad MSChemical Noise Limited
Analyte Signal IncreasedChemical Noise IncreasedS/N does not improve
Page 38Page 38
Extractor Source Increases Signal for All IonsEI: spectrum of analyte can also include ions from matrix, column bleed, gases, etc.
Q1 SIM isolate precursor before CID
Product 1Product 3
Product 2
CID +Q2 SIM
chemical noise from these other ions is eliminated
MS/MS still eliminates chemical noise
Page 39
Why Is the New Extractor Source Valuable for MS/MS?
Single Quad MSChemical Noise Limited
Analyte Signal IncreasedChemical Noise IncreasedS/N does not improve
Triple Quad MS/MSProduct Ion Signal Limited
Product Ion Signal IncreasedChemical Noise Does NOT IncreaseS/N (and LOD) are improved
Page 40
Unique Entrance Lens Eliminates Pre-filter
The last lens, which extends into the heated monolithic quadrupole, optimizes coupling of the source to the analyzer. Voltage on this lens is dynamically ramped synchronously with analyzer voltage to focus maximum ion signal for every m/z into the center of the quadrupole field.
Improved lens
Page 41
7000B MS/MS: New Ramped-Iris Detector
New Ramped Iris Triple-Axis Detector
Ramped-iris improves transfer of ion from the exit of the second analyzer into the Triple-Axis detector
Page 42
High Performance Triple-Axis Detector with NewDynamically Ramped Iris
• Maximum reduction of neutral noise
– The combination of helium quench gas and the Triple-Axis design delivers ultra low noise
• Long life and high linearity– Triple channel electron multiplier
• “Gain Normalization” corrects tune file for detector aging
– Consistent long term method sensitivity
• Design proven in the 5975C MSD
hyperbolic quartztransmission
quadrupoleanalyzer
triple channelelectron multiplier
high energy dynode
steering rod
ion beamX
Y
OffsetZshield for
secondary particles
triple channelelectron multiplier
high energy dynode
steering rod
ion beamXX
YY
OffsetZ
OffsetZshield for
secondary particles
Page 43
• Improved high mass peak shape• Improved mid to high mass sensitivity
Mass Iris (V) Signal Increase
69 -1 1.0X
131 -8 1.1X
264 -13 1.5X
414 -20 2.5X
502 -30 2.8X
614 -35 4.0X
Ramped-Iris Triple-Axis Detector
Page 44
7000B GC/MS/MS Specifications
OFN = octafluoronaphthaleneBZP = benzophenone
EI SRM > 500:1 for 100 fg OFNPCI SRM (CH4) > 50:1 for 100 fg BZP
EI MRM Pk Area RSD < 8% for 100 fg OFN
EI Scan > 300:1 for 1 pg OFNNCI SIM (CH4) > 200:1 for 10 fg OFN
Installation Checkout Specifications
Typical Precision
Typical Sensitivity
Page 45Page 45
ColumnBackflush:
Many Wonderful Advantages for GC/MS/MS of
Complex Matrices
Page 46Page 46
What Real Value Does High Performance EPC Have If Matrix Carryover Destroys Rt Precision?
m in5 . 3 5 . 4 5 . 5 5 . 6 5 . 7 5 . 8 5 . 9 6 6 . 1 6 . 2
N o r m .
2 0 0 0
4 0 0 0
6 0 0 0
8 0 0 0
1 0 0 0 0
1 2 0 0 0
1 4 0 0 0 10 Runs without BackflushingRetention times shift ~4-5 secduring 10 runs
m in5 . 3 5 . 4 5 . 5 5 . 6 5 . 7 5 . 8 5 . 9 6 6 . 1
N or m .
0
2 0 0 0
4 0 0 0
6 0 0 0
8 0 0 0
1 0 0 0 0
1 2 0 0 0
1 4 0 0 0
1 6 0 0 0
10 Runs with Backflushing
Page 47Page 47
Productivity: CFT Backflush
• More analyses per hour . . . – Shorter analysis cycle time (time from injection to injection) – Reduced requirement for sample preparation
• with consistently better results . . . – Quantitative: accuracy, precision, detection limits, linear range– Qualitative: response ratios, confirmatory retention times, library
searches (eliminate false negatives and false positives)
• while using less operator time for each analysis.– Less time performing maintenance on column and ion source
Page 48Page 48
Multiple Backflush ConfigurationsMultiple Backflush Devices
• Backflush from the start of the column– Backflush guard column only
• Backflush from the end of the column– Backflush entire column
• Backflush from the middle of the column– Backflush a percentage of the column– Example: at tee between two 15 m columns
Purged UltimateUnionNew Pressure
Controlled Tee Kit
Page 49Page 49
Backflush – Mid-Column (Backflush Mode)
Vent
Pressure / Flow
Controller
15-m HP-5ms (0.25mm id x0.25um)
15-m HP-5ms (0.25mm id x0.25um)
5975C MSD or 7000B MS/MS
7890A GC
Split/Splitless Injection Port
Decrease inlet pressure during backflush
Pressure Controlled Tee
Page 50Page 50
Backflush with Purged Ultimate Union: Lipid Peroxidation Products in Blood
App Note 5989-9359EN
4-hydroxy-2,3-nonenal (HNE), an indicator of oxidative stress, and its metabolite, 1,4-dihydroxynonene (DHN)
PCI with NH3
Late eluting peaks were quickly contaminating the source
Page 51Page 51
Backflush with Purged Ultimate Union: Loss of Response without Backflush
without backflush
with backflush
App Note 5989-9359EN
Page 52Page 52
Backflush with Purged Ultimate Union: Loss of Response without Backflush
without backflush
with backflush
These were the first backflush results; the system is getting clearer
App Note 5989-9359EN
Page 53
Page 53 Pesticide Analysis with New Agilent Jet Stream Technology
Break for Questions
For questions, at break please dial *1 on your phone, or type onto the Q&A box at any time duringthe presentation.
December 12, 2008
Page 54Page 54
MassHunter Software
Page 55Page 55
MassHunter
Quantand
Qualmodules
MassHunter
Quantand
Qualmodules
MassHunter SoftwareCommon Workflow for all Mass Spec. applications
LC/MS Acquisition ModuleTriple Quad LC/MS, LC/TOF, LC/QTOF
LC/MS Acquisition ModuleTriple Quad LC/MS, LC/TOF, LC/QTOF
GC/MS Acquisition ModuleTriple Quad GC/MS
GC/MS Acquisition ModuleTriple Quad GC/MS
MassHunter formatMassHunter format
Translator
Translator
ChemStationSingle Quad LC/MSChemStation
Single Quad LC/MS
ChemStationSingle Quad GC/MS (MSD)
ChemStationSingle Quad GC/MS (MSD)
Page 56Page 56
MassHunter Data Analysis ApplicationsOne software for all your Agilent MS applications
•Target Compound Analysis• High throughput quantitation
•Quantitative Analysis •Qualitative Analysis•Discovery & Method Development• Find features• Identify compounds
Page 57Page 57
Quantitative Analysis View
Results Information
Page 58Page 58
Quantitative Analysis View
Compound Information
Results Information
Page 59Page 59
Quantitative Analysis View
Compound Information
Results Information
Calibration Information
Page 60Page 60
Results Information:All Results for batch in one view
• Easy visualization for all results– Customize columns for desired data
• Easy Navigation– Compound and Calibration views change when new compound is selected– Reduce data by filtering on sample type, compound, time segment, and outlier
Page 61Page 61
Results Information:Detailed compound view
•Detailed compound information is easily seen
Page 62Page 62
Results Information:Highlight outliers
•Compounds with results outside specified ranges are highlighted• 30 metrics are available• Custom Calculation is possible• More to be added
– i.e. Peak metrics
Page 63Page 63
Results Information:Highlight outliers
•Compounds with results outside specified ranges are highlighted• 30 metrics are available• Custom Calculation is possible• More to be added
– i.e. Peak metrics
Page 64Page 64
Results Information :Simple Metrics – Plot Columns
•Ability to see trends in a batch•Ability to diagnose quantitation problems easily• Missing ISTD amount• Retention time drift
Page 65Page 65
Compound Information:All relevant information in one view
CompoundIntegration
QualifierInformation
SpectrumInformation
ISTDIntegration
ISTD QualifierInformation
ISTD SpectrumInformation
Page 66Page 66
Calibration Curve Views
Can include ISTD, QC, and CC data points on curvePoint information is available as pop-up
QC point
Page 67Page 67
Calibration Curve Assistant
Display all possible Calibration Curve fit options•Review R2, Standard error, or Max % Residual•Can sort, filter, or browse options
Page 68Page 68
Calibration Curve Assistant
Filter possible calibration curves based on requirements
Compare selected calibration fit (black) with proposed (blue).•Linear•All points•Ignore origin
Page 69Page 69
Reporting and Data Transfer
Use of XML results format• Easy data exchange
– LIMS data exchange– Copy/Paste views from MassHunter
Microsoft Excel reporting engine• Powerful Features
– Excel Macros and formulas can be created for custom result manipulation
• Flexible Reports and Layouts– Custom Layouts and graphics
properties.• Preconfigured report template
examples
Page 70Page 70
Productivity of GC/MS/MS
• Sample complexity forces the change from MS to MS/MS– Could not meet required LOD/LOQ in real samples
– Could not avoid interferences from matrix ions (isobaric ions)
Page 71Page 71
Productivity of GC/MS/MS
• Sample complexity forces the change from MS to MS/MS– Could not meet required LOD/LOQ in real samples– Could not avoid interferences from matrix ions (isobaric ions)
• Practical aspects of productivity– MS/MS selectivity for less sample preparation– MS/MS sensitivity for less injected on-column
• In turn, less maintenance– MS/MS selectivity for faster analysis cycles
• MS/MS selectivity lessens the requirement for column selectivity• Less bakeout or backflush may be needed
– Caution: selectivity is a type of ‘blindness’ and can be misleading
– MS/MS selectivity for faster and easier peak integration• What can be easier than a flat baseline and a single peak
Page 72
Page 72 Pesticide Analysis with New Agilent Jet Stream Technology
Break for Questions
For questions, at break please dial *1 on your phone, or type onto the Q&A box at any time duringthe presentation.
December 12, 2008
Page 73Page 73
Thank You for Attending Today’s eSeminar
Page 73
Terry L. Sheehan, Ph.D.GC/MS/MS Product Manager
Agilent Technologies, Santa Clara, CA