Albumin Analysis by Potentiometric Method

Albumin concentration

(ppm)

Epa Epc Ipa Ipc

(V vs. Ag/AgCl(0.5MKCl)) (A)

0 0.291 0.070 53.87 -40.42

0 0.292 0.070 49.96 -47.43

1 0.445 -0.017 31.69 -34.26

2 - - - -

4 - - - -

6 - - - -

8 - - - -

10 - - - -

Table : The relationship of peak current and albumin concentration

Fig 2. The cyclic voltammogram of the selectivity

WE:Au wire, WE area: 0.0113 cm2, RE: Ag/AgCl(0.5M), CE: Pt, Electrolyte: 0.5 M KCl 50 ml(20 mM K4Fe(CN)6+20 mM K3Fe(CN)6), Potentiostat: CHI-405, Scan range: -0.2~0.6 V, scan rate: 0.05 V/sec

-0.2 0 0.2 0.4 0.6Potential (V vs. Ag/AgCl(0.5M KCl))

- 8 0

- 4 0

0

4 0

8 0

Cu

rren

t (

A)

nonealbuminEPHADANEP

Epa Epc Ipa Ipc

(V vs. Ag/AgCl(0.5MKCl)) (A)

None 0.275 0.109 54.51 -48.12

5ppm Albumin 0.246 0.153 17.89 -16.89

0.0025ppm EP 0.246 0.139 88.08 -64.24

3.45ppm HA 0.259 0.131 60.88 -55.65

1.125ppm DA 0.246 0.124 74.18 -55.79

0.015ppm NEP 0.252 0.093 70.79 -43.68

Table Ⅱ : The relationship of peak current and albumin concentration

Fig 3. The cyclic voltammogram of Au wire electrode

WE: Au wire, WE area: 0.0113 cm2, RE: Ag/AgCl(0.5M), CE: Pt, Electrolyte: 0.5 M KCl 50 ml(20 mM K4Fe(CN)6+20 mM K3Fe(CN)6), Potentiostat: EG&G, Scan range: -0.2~0.6 V, scan rate: 0.02 V/sec,albumin 在電解液中加入

-0.2 0 0.2 0.4 0.6Potential (V vs. Ag/AgCl(0.5M KCl))

- 4 0

- 2 0

0

2 0

4 0

Cu

rren

t (

A)

album in concentration0 ppm1 ppm2 ppm

Albumin concentration

(ppm)

Epa Epc Ipa Ipc

(V vs. Ag/AgCl(0.5M)) (A)

0 ppm 0.248 0.142 36.70 -36.90

1 ppm 0.236 0.126 37.30 -32.70

2 ppm 0.234 0.130 39.90 -32.70

Table Ⅲ : The relationship of peak current and albumin concentration