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ALL ABOUTCOLLAGEN

PRODUCED & MARKETED BY ROUSSELOT

Collagen Peptides for a Healthy Lifestyle

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SUMMARY4/5 What is collagen

Collagen and its role

in the human body

Structure of collagen

What happens when we age?

6/7 Different forms of collagen

Digestibility and bioavailability

8/9 Dual action effect

Collagen is a unique protein

10/13 Collagen peptides’ health benefits

14/15 How to replenish collagen

FAQ

16/17 Peptan, the world’s leading

collagen peptide brand

18/19 Recipes for a healthy lifestyle

Beet and berry smoothie

Miso soup

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What is collagenCollagen is a protein found in

animals and it is made up of amino

acids. Since ancient times, collagen

has been known to benefit human

health. For centuries, people have

been consuming collagen in the

form of bone broth to support their

joint and bone health and in more

recent times, to promote skin

beauty.

Collagen and its role in the human body

Structure of collagen

Collagen is the major

protein in the

connective tissues of

the body. It holds all

living tissues

together and ensures

the integrity, elasticity and

regeneration of skin, cartilage

and bones. It is the most abundant protein,

representing almost 30% of all human

protein content.

Collagen distribution in human body (by weight ratio of dry mass)

Bones 90%(organic

bone mass)

Tendons 85%

Joint cartilage 70% Ligaments 70%

Skin 75%

Tendinous muscles 6%

Collagen is a triple helix of

alpha-chains of amino acids

which build strong fibers used

for the body’s structure.

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What happens when we age?

Illustration of skin structure

Collagen production in our body peaks around

the age of 20, and as we age, the body’s

production of collagen metabolism slows

down causing a gradual deterioration of

collagen fibers in all connective tissues.

This degradation process will affect different

parts of our body, for example in the skin as

shown in below image.

Skin:As skin cells become less active, the collagen

network that provides skin firmness and

structure breaks down. Skin

becomes dehydrated and thinner,

plus lines, wrinkles and deep

furrows start to appear.

Bones:Bone turnover becomes

imbalanced, that means there is

more bone loss than bone formation.

This causes bones to become more fragile and

easier to break.

Joints:Lower levels of collagen and other matrix

components caused by aging can lead to

loss of cartilage and joint function.

This results in joint discomfort.

Muscles:A gradual loss of muscle

mass and strength caused by

aging can affect balance, gait

and overall mobility.

State of full collagen

Der

mis

Epid

erm

is

Aging causes collagen in dermis to damage and fragment

Der

mis

Epid

erm

is

Collagen

Fibroblastscells

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Different forms of collagenCollagen is a safe and natural ingredient which

can be obtained in different grades. It can be

found in foods such as bone broth or

gelatin-based desserts.

The controlled breakdown of native collagen

with the help of enzymes produces collagen

peptides.

The solubility, absorption and digestibility vary

among different grades of collagen.

The much smaller and easily digestible collagen peptides are produced by controlled

enzymatic hydrolysis

Grade Form SolubilityAbsorption & digestibility

Application examples

Native collagen

Insoluble NoneMedical materials, collagen casings

Gelatin Medium LowGelatin desserts,

confectionery

Collagenpeptides

High HighDietary supplements,

functional foods

Enzymatic hydrolysis

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Digestibility and bioavailabilityCollagen peptides are not exactly the same as

gelatin. Both originate from collagen and are

proteins made of amino acids. Collagen

peptides are small molecules produced by an

enzymatic hydrolysis and they have a much

lower molecular weight than native collagen

peptides. Collagen peptides do not have the

gelling functionality of gelatin and are soluble

in cold water. They are highly digestible and

bioavailable and are used in dietary

supplements and functional foods because of

their proven health benefits.

Mouth Mechanical breakdown

Denaturation by gastric acid and first hydrolysis

Main hydrolysis with pancreas enzymesAbsorption

Residual protein (< 10%)No absorption

Stomach

Small intestine

Colon

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3

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Intact protein

Denatured and degraded

protein

Peptides≤ 10 amino acids,free amino acids

Studies have demonstrated that over 90% of the peptides are digested

and absorbed within a few hours after meals.

This rapid absorption ensures an effective delivery of the essential

peptides and amino acids to their site of action within the body.

Ichikawa, S. et al., 2010, Hydroxyproline-containing dipeptides and tripeptides quantified at high concentration in human blood after oral administration of gelatine hydrolysate. International Journal of Food Sciences and Nutrition, 61(1):52-60

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Dual-action effect

Role of vitamin CVitamin C is a co-factor which supports the

production of collagen in the body.

Therefore, it is important to ensure that you

have sufficient vitamin C in your diet to boost

the collagen synthesis.

Proteins are essential nutrients for our body.

They are made from long chains of amino

acids. Collagen peptides are proteins that are

highly bioavailable.

Consumption of collagen peptides will help

supply your body with amino acids needed as

building blocks to renew tissues such as skin,

bones and joints.

It has been proposed that collagen peptides

may act as a messenger to the cells and

trigger the synthesis and reorganization of

new collagen fibers, therefore supporting skin

tissue structure.

Absorbed amino acids help supply nutrients to your body.

Collagen peptides

NUTRIENTS

Digestion and absorption

MESSENGER FUNCTION

Collagen peptides

Collagen

Fibroblastcells

Illustration of skin structure

Specific amino acids such as hydroxyproline contained in the absorbed collagen peptides may act as messengers

to activate fibroblast cells.

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Collagen is a unique protein with key amino acidsCollagen peptides are characterized by a high

level of the key amino acids: glycine and

proline/hydroxyproline. These represent

around 50% of the total amino acid content.

Hydroxyproline is unique to collagen. The

amino acids in collagen are the same as found

in skin, joints and bones.

This very specific composition of amino acids

provides collagen peptides with unique

biofunctional properties that cannot be found

in other protein sources.

20%

25%

11%8%

16%

12%

8%

GlycineOthers - Aspartic acid, Serine ...

Essential amino acids

Alanine

ArginineGlutamic acid

Proline/ Hydroxyproline

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Collagen peptides’ health benefitsSkinCollagen makes up 75% of the skin’s dry

mass skin content. As a key component of the

skin’s structure, collagen fibers provide the

infrastructure for elastin, which maintains the

skin’s elasticity, and for hyaluronic acid to trap

moisture.

Skin dryness and an accelerated

fragmentation of the collagen network in the

dermis are hallmarks of skin aging. The

causes can be natural aging, UV from sunlight,

pollution, lifestyle and nutritional choices.

Collagen matrix

Line

Wrinkle

Fold

Fat

Collagen’s effects on skin beautyClinical trials showed that collagen peptides

(Peptan) consumption effectively supports the

intrinsic collagen network of deeper skin

layers1: the fragmentation of collagen in the

deep layers of the skin was reduced already

after 4 weeks and decreased by 31% after

12 weeks. At the same time, the density of

the collagen layer increased, providing more

strength to the skin.

Peptan further increased skin moisture by

28% after 8 weeks. This restructuring of the

skin is key to bringing about anti-aging benefits

and a more youthful appearance.

Peptan is also proven to stimulate collagen

production and moisture by increasing the

amount of water-binding hyaluronic acid in the

epidermis.

Illustration of skin structure

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OSTEOBLASTS Star-shaped cells

A

OSTEOCLASTS Round black cells

B

BoneRepresenting around 90% of organic bone

mass, collagen is a protein that provides

bones their soft framework. Calcium

phosphate is the mineral that adds strength

and hardens the framework. Together,

collagen and calcium give bones their strength

and at the same time flexible enough to

withstand stress.

A cycle of continuous bone formation and

breakdown replaces approximately 15% of

bone mass in healthy adults each year.

When this balance tips towards faster bone

loss and slower bone formation, the bone

density decreases leaving the bones more

fragile.

The image on the left shows bone cells that are cultured in the presence of Peptan (A) or a control protein (B). Peptan stimulates the development of bone forming cells (osteoblasts) visible as star-shaped cells much more than the control protein. Bone resorbing cells (osteoclasts) are not affected by Peptan or the control protein.

Collagen’s effect on bone healthCollagen peptides (Peptan) are demonstrated

in scientific studies to promote bone health by

stimulating bone renewal, maintaining bone

mineral density and improving bone solidity

and strength.2,3,4,5

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JointsCollagen fibers make up to 70% of dry

cartilage mass and are responsible for its

structure and strength.

Aging and high impact sports can cause wear

and tear to the cartilage. This can lead to joint

discomfort, tenderness, stiffness and locking.

Collagen’s effect on joint healthCollagen peptides (Peptan) have been proven

to stimulate cartilage cells synthesis,

producing increased aggrecan and collagen.6

A clinical study showed that an intake of Peptan

can reduce joint discomfort and improve joint

flexibility and function.7

The Peptan treatment resulted in a significant

decrease of the WOMAC score linked to an

improvement of 37% in joint pain score, 44%

in stiffness score and 22% in function score. A

recent in-vivo study showed that Peptan

uniquely combines anti-inflammatory effects

with clear joint structure benefits, as it

supports cartilage regeneration.8

MuscleSarcopenia is the age-related loss of muscle

mass and function. Exercise and adequate

intake of easily digestible protein is vital to

counteracting the condition’s effects.

Collagen’s effect on musclesCollagen peptides are bioactive proteins

promoting muscle regeneration. Study

showed that collagen peptides can help

maintain nitrogen balance and preserve lean

body mass in elderly adults.9 Collagen peptide

supplementation in combination with

resistance training has been shown to improve

body composition and increase muscle

strength in elderly sarcopenic men.10

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Collagen peptides such as Peptan is a bioactive

and pure protein, and as a protein it can help

contribute to different health benefits. Protein

is a vital part of the diet and the WHO

recommends a mean daily intake of 0.8g/kg

body weight of good quality protein.

Prevent injuries Repetitive, high-impact movements can exert

excessive stress on joints and even damage

the cartilage, tendons and ligaments. Collagen

peptides can support connective tissues and

limit discomfort as well as the risk of any

joint-related injury.11,12

Post-workout recoveryA high protein diet containing collagen

peptides just after exercise may enhance

muscle anabolism, replace lost proteins, help

maintain and restore the protein content of

the muscles while also reducing the recovery

time.

Satiety effectProviding a high satiety effect, collagen

peptides are an excellent protein source to

control appetite and moderate cravings.13

Other benefits of collagen peptides

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How to replenish collagenDaily intakeIn order to continuously boost the production

of collagen in the body, it is recommended to

consume collagen peptides on a daily basis.

If you have a medical condition or are

pregnant, please consult your medical doctors

or health care professionals.

Dietary supplementsCollagen peptides are available in different

forms for daily intake.

They are available in the form of drinks,

powders, tablets and more, depending on

your preference and lifestyle.

Functional foodsNeutral in taste and providing multiple

functional benefits, different types of foods

and beverages can be enriched with collagen

peptides to boost their health benefits.

Functional foods and beverages such as dairy

and nutritional bars are also good sources of

collagen peptides and can be alternatives to

dietary supplements.

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FAQAre collagen peptides safe?Collagen peptides such as Peptan are a pure

and bioactive protein, derived from a 100%

natural source and free from any side-effects.

What is the source of collagen peptides?Collagen has a long history of use in foods,

mainly in the form of gelatin found in

gummies and of the desserts. Collagen is also

naturally present in high amounts in some

cooked foods, e.g. bone broth and in the skin

of fish and chicken.

Collagen peptides are extracted from high

quality raw materials, are purified and dried to

produce a pure protein powder.

How much time is needed to see the first results?Most studies showed positive health effects

on skin and joints within 1-3 months of daily

intake. However it is vital to keep up a regimen

of daily collagen intake to ensure efficient

metabolism and optimal effects. Can collagen peptides simulatenously support multiple health benefits?Absolutely, collagen peptides like Peptan are

backed by published scientific evidence for

their benefits on skin beauty and joint health.

Further indications from preclinical models

suggest that Peptan helps to maintain healthy

bones. Thus, Peptan is a holistic solution to

maintain the health of musculoskeletal system

and also support skin beauty.

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What is Peptan® Peptan collagen peptides are Type 1 Collagen

peptides. Peptan is a high-purity and natural

product, containing more than 97% protein

(on a dry weight basis).

Peptan is optimized for high bioavailability and

activity, and its hydrolyzed form makes it

easily digestible. Peptan is a bioactive

ingredient that has been specifically developed

to deliver multiple health benefits and

functional properties.

Numerous scientific studies, including in vitro,

in vivo and double-blind, placebo-controlled

clinical studies, have demonstrated Peptan’s

ability to promote healthy living and its

proven benefits in key areas of healthy

aging, joint and bone health, skin beauty and

sports nutrition.

Easy to usePeptan is a bioactive protein in powder form. It

is neutral in taste and odor, highly soluble and

heat-resistant. These characteristics make

Peptan easy to be incorporated into a range of

formulations from dietary supplements to

functional foods and beverages such as

confectionery, dairy and more.

Peptan can be used as a supplement or

directly as a powder addition to foods and

drinks.

Peptan is compatible with other active

ingredients such as vitamins and minerals.

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Quality firstAs a premium collagen peptide, Peptan is

100% natural, safe and free from any

preservatives or additives. Peptan is

manufactured by Rousselot’s state-of-the art

certified plants in France and Brazil and meets

the highest international food and quality

standards with full traceability

throughout the process. Peptan

can be provided under Halal

or Kosher certification.

Scientific references:

1. Asserin, J. et al., 2015, The effect of oral collagen peptide supplementation on skin moisture and the dermal collagen network: evidence from an ex vivo model and randomized, placebo-controlled clinical trials. Journal of Cosmetic Dermatology, 14:291-301. doi: 10.1111/jocd.12174

2. Guillerminet, F. et al., 2010, Hydrolyzed collagen improves bone metabolism and biomechanical parameters in ovariectomized mice: An in vitro and in vivo study. Bone, 46:827-834

3. Guillerminet, F. et al., 2012, Hydrolyzed collagen improves bone status and prevents bone loss in ovariectomized C3H/HeN mice. Osteoporosis International, 23(7):1909-1919

4. Daneault, A. et al., 2014, Hydrolyzed collagen contributes to osteoblast differentiation in vitro and subsequent bone health in vivo. Osteoarthritis and Cartilage, 22:S131

5. Daneault, A. et al., 2015, Biological effect of hydrolyzed collagen on bone metabolism. Critical Reviews in Food Science and Nutrition, 10:1040-8398

6. Rousselot unpublished data, 2011 7. Jiang, J.X. et al., 2014, Collagen peptides improve knee

osteoarthritis in elderly women: A 6-month randomized, double-blind, placebo-controlled study. Agro Food Industry Hi Tech, 25: 19-23

8. Dar, Q.A. et al., 2016, Oral hydrolyzed type 1 collagen induces chondroregeneration and inhibits synovial inflammation in murine posttraumatic osteoarthritis. Osteoarthritis and Cartilage, 24:S532–S533

9. Hays, N.P. et al., 2009, Effects of whey and fortified collagen hydrolysate protein supplements on nitrogen balance and body composition in older women. Journal of the American Dietetic Association, 109:1082-1087

10. Zdzieblik, D. et al., 2015, Collagen peptide supplementation in combination with resistance training improves body composition and increases muscle strength in elderly sarcopenic men: a randomized controlled trial. British Journal of Nutrition, 114 (8):1237-1245

11. Shaw, G. et al., 2016, Vitamin C-enriched gelatin supplementation before intermittent activity augments collagen synthesis. American Journal of Clinical Nutrition, doi:10.3945/ajcn.116.138594

12. Baar, K. 2015, Training and nutrition to prevent soft tissue injuries and accelerate return to play. Sports Science Exchange, 28(142):1-6

13. Veldhorst, M.A. et al., 2009, A breakfast with alpha-lactalbumin, gelatin, or gelatin TRP lowers energy intake at lunch compared with a breakfast with casein, soy, whey, or whey-GMP. Clinical Nutrition, 28(2):147-15

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Recipes for a healthy lifestyle

Beet and berry smoothieA healthy and colorful way to start your day. Delicious and loaded with Peptan collagen peptides,

antioxidants, vitamins and lots of other nutrients, this beet and berry smoothie can help boost

health and fitness, and promote a radiant and fabulous looking skin too.

INGREDIENTS

Serves: 4

• 150g cooked red beets, chopped

• 50g frozen raspberries

• 50g frozen blackberries

• 1 large banana

• 225g Greek yogurt

• 50g Peptan collagen peptides

• 250ml apple juice

• 4 fresh mint leaves (as edible garnish)

1. Combine all ingredients in a blender.

2. Blend on high speed until smooth.

3. Pour in glasses and garnish

with mint leaves.

4. Serve immediately.

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Miso soupMiso soup is a traditional Japanese soup, the intense umami flavor nourishes and warms you

from the inside. High in protein, vitamins and with added Peptan, this miso soup is not only a

tasty comfort food, but also helps to support your mobility.

INGREDIENTS

Serves: 4

• 1 liter dashi stock or fish stock

• 20g wakame seaweed or dried nori

• 75g red miso paste

• 150g silken tofu, cubed

• 4 spring onions, finely sliced

• 50g Peptan collagen peptides

1. Place the dashi or fish stock in a large saucepan over medium heat. Bring to the boil. Reduce

heat to low. Add the seaweed and and the tofu cubes, simmer gently for 5 minutes.

2. Place the miso paste in a small bowl. Add a little of the dashi, stirring until miso dissolves.

Add the miso mixture to the saucepan and gently stir to combine. Add Peptan slowly in the

soup, stir for 1 minute until dissolved and bring the soup to a simmer. Remove from heat.

3. Sprinkle the spring onions and serve in bowls.

Enjoy!

FOR MORE RECIPE AND INSPIRATIONS FOLLOW US ON PEPTAN SOCIAL MEDIA.PeptanbyRousselot @Peptan_Global

Rousselot Headquarters:

Rousselot B.V. Kanaaldijk Noord 20 5691 NM Son The Netherlands

Phone: +31 499 364 100 peptan@rousselot.com

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peptan.com PeptanbyRousselot @Peptan_Global Collagen Peptides

PRODUCED & MARKETED BY ROUSSELOT

Rousselot makes no representation or warranty, whether expressed or implied, of the accuracy, reliability, or completeness of the information, nor does it assume any legal liability, whether direct or indirect, of any information. Use of this information shall be at your discretion and risk. Nothing herein relieves you from carrying out your own suitability determinations and tests and from your obligation to comply with all applicable laws and regulations and to observe all third party rights. This product is not intended to diagnose, treat, cure, or prevent any disease. You should always consult your medical provider when using the product together with medical treatments, diets or fitness programs. The uses and claims for Rousselot’s products recommended in the brochure should be adapted to the current local regulatory environment. This statement has not been evaluated by the Food and Drug Administration.

At Peptan, we believe in our science. Peptan

provides multiple health benefits by acting as

building blocks for healthy joints, bones and

muscles as well as skin. Globally recognized as

the leading collagen peptides brand, Peptan

consistently delivers the highest quality and

support to meet your innovation and formulation

needs. Peptan is the one bioactive ingredient that

brings you a step closer to a healthier tomorrow.

VISIT PEPTAN.COM OR SCAN THE QR CODE TO FIND OUT

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