ISSN 1344- 3941 CODEN NICKA3

Alnimal S5cience Journal

Vol70 No10 October 1999

Japanese Society of Animal Science

B~mim~~~ tt~~A Bliim~~

Animal Science Journal

Official Journal of Japanese Society of Animal Science

President Kohkichi UEHARA

Vice president Tsutomu KONNO Hideo YANO

Editor-in-Chief S SAKAI

Editorial Board T HARIGAY A H ITABASHI N KAKI-ICHI T KAMADA

Y KANAMARU Y KARASAWA S KOBAYASHI Y SASAKI S SUGITA

Executive Editors T TANAKA H TOMOGANE

Membership includes subscription to the Journal (No 1-6 in English No 7-10 in Japanese)

and the privilege of submitting reports for publication The annual membership fee is 8000 yen

Foreign subscribers for the Journal (No 1-6) have the privilege of submitting reports for

publication The annual subscription rate is 4000 yen

All correspondences concerning this Journal should be addressed to Japanese Society of

Animal Science 201 Nagatani Corporas Ikenohata 2-9-4 Taito-ku Tokyo 110-0008 Japan

Abbreviation Anim Sci J

Formerly The Japanese Journal of Zootechnical Science (Vol 1-62)

Animal Science and Technology (Vol 63-69)

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Phone (978) 750 - 8400 Fax (978) 750-4744

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Animal Science Journal

Official Journal of Japanese Society of Animal Science

President Kohkichi UEHARA

Vice president Tsutomu KONNO Hideo YANO

Editor-in-Chief S SAKAI

Editorial Board T HARIGAY A H ITABASHI N KAKI-ICHI T KAMADA

Y KANAMARU Y KARASAWA S KOBAYASHI Y SASAKI S SUGITA

Executive Editors T TANAKA H TOMOGANE

Membership includes subscription to the Journal (No 1-6 in English No 7-10 in Japanese)

and the privilege of submitting reports for publication The annual membership fee is 8000 yen

Foreign subscribers for the Journal (No 1-6) have the privilege of submitting reports for

publication The annual subscription rate is 4000 yen

All correspondences concerning this Journal should be addressed to Japanese Society of

Animal Science 201 Nagatani Corporas Ikenohata 2-9-4 Taito-ku Tokyo 110-0008 Japan

Abbreviation Anim Sci J

Formerly The Japanese Journal of Zootechnical Science (Vol 1-62)

Animal Science and Technology (Vol 63-69)

3L AB 83 1pound ~ --rs yen~

A shyplusmn BJ $ A B ~ pound ~ z 9E n

plusmnm~~~~ i it ill D

m~~~ ~ m i 1ttt j 1 sect

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T107 - 00S2 )7( rtSi-JlxiJJdl( 9-6- 41 j-1ti t Iv iwXftlirW0 TEL 03-347 5- 5618 FAX 03 - 3475 - 5619

Emiddotmail kammorimsh biglobenc jp

7 I J tJ 0lltlql~fj It -ltgt HiI~ -gt L -C lt ((Ipoundill L l ~ ~

Copyright Clea rance Center Inc (CCC)

222 Rosewood DriYe Danyers MA 01923 USA

Phone (978 ) 750- 8400 Fax (978) 750- 4744

hllp wwwcopyrigh lcom

Notice about photocopying

In order to phOLOlOPY a ny work rrom this publica lion you Or

you r orga nizal ion must obtain permission from the followi ng

organization which has been delegated ror copyr ight ror clearance

by the copyright owner of this publication

Exeept iD the USA

Japan Academic Association for Copyr ight Clea rancc (JAACC)

41-6 Akasaka 9-c home Minato-ku Tokyo 107-0052 Japan

TEL 81- - 3475-5618 FAX 8 1-3 - 3475 -5619

E-mail kammorimshbiglobe ncjp

In the USA

Copyright Clearancc Cent cr Inc (CCC)

222 Roscwood Drive Darers MA 01923 USA

Phone (978) 750 - 8400 Fax (978) 750-4744

http ww wcopyrighleom

Sf- ~ 11 $ 10 ~

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Animal Science Journal

Vol 70 No 10 October 1999

Contents

Regular Papers (in Japanese with English Abstract)

Studies on the Recovery of Genomic DNA and Functional Genes from Mammalian Pelt Specimens

Asano Y Uekita T Kasai K Endo H Yamada T Saburi S Yamanouchi K Tojo H Natori M Tachi C 1351

Ovarian Morphological Structures and Plasma Steroid Hormone Profile in Cows Treated with Prostaglandin Fla after Embryo Collection

Ushinohama K Kamimura S Hamana Kmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddot middotmiddot middotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddot 1363

Assisted Hatching of In vitro-produced Bovine Embryos Amano T Yamanaka M Itagaki Y 1370

Effects of Age and Sexual Experience at Pairing on the Reproductive Performance in Mongolian Gerbils (Meriones unguiculatus)

Kai 0 J377

Butyrolactone-I Inhibits Germinal Vesicle Breakdown of Porcine Oocytes Cultured in vitro

Nishimoto N Nagai T Yamauchi N Takenouchi N Geshi M Hashizume T Masuda H J384

Effects of Dietary Protein Degradability on Nitrogen and Energy Utilization of Lactating Cows

Nakai F Hirashima Y Ueda K Agung Purnomoadi Higuchi K Enishi 0 Terada Fmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddotmiddot J390

Accumulation of r-Aminobutyric Acid into Cheddar Cheese by Addition of Heat-treated Lactococcus Lactis Cell

Nomura M Someya Y Furukawa S Suzuki I 1397

Meat Quality and the Color Changes of Jersey Cows Kuriki T Izumimoto M Miyamoto T J403

Effect of Handling Treatment during Three Days after Birth on the Subsequent Reaction to Humans in Japanese Black Calves

Kosako T Imura T J409

Behavioral Characteristics of Japanese Black Calves during Leading Training in the First Seven Days after Birth

Kosako T Imura T J415

Abstracts (in Japanese) Animal Science Journal Vol 70 No 5 J421

News and Announcements for Japanese Members J430

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Anim Sci 1 70 ( 10) 1390-1396 1999 1390

Table 1 Ingredients and chemical composition of diets

Treatment Item

LD HD

Ingredients ( DM)

Roughage

Italian ryegrass wafer

Concentrate

Flaked corn

Barley grain

Beet pulp

Alfalfa meal

Soybean meal

Fish meal

Brewers grains

Soybean hulls

Calcium salts of fatty acids l)

Molasses

CaHP04

Mineral and vitamin supplements2)

Chemical composition ( DM or MJkgDM)

DM

CP

VIP ( CP)))

EE

NDF

GE

346

14 8 135

98

90 40

50

41 2 4

06

20

O 1

869

183 429

49

388 18 7

34 2

E6

02

~~ 6

8 1

12 6

7 2

15

19

O 9

O 1

868 18 2 32 3

4 7

39 1

185

LD low degradable protein HD high degradable protein DM dry matter CP crude protein VIP undegraded intake protein EE ether extracts NDF neutral detergent fiber

GE gross energy I) Megalac Volac Ltd

2) Trace minerals (contained 60 Zn 50 Mn 50 Fe 10 Cu and 01 I) vitamin A D E mixture (contained 10000 IVg vitaminA 2000 IVg vitaminD) and DL-a tocopherol acetate IOmgg) ) Calculated from Japanese Feeding Standard for Dairy Cattle (1994)

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Anim Sci J 70 ( 10) bull J390-J396 1999 J391

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Table 2 Effects of dietary protein degradability on milk production and composition

Treatment Item SE

LD HD

Body weight (kg) 599 593 3 14 DM intake (kgday) 19 3 193 049

Roughage 66 6 7 0 05 Concentrate 12 7 126 003

Milk yield (kg day) 349 348 O 19 4 FCM yield (kgday) 31 2 332 0 38 1

Milk

Fat () 3 32 3 70 006 Fat (kgday) 1 15 1 28 002+

Protein () 309 2 97 002 Protein (kgday) 108 104 0003 SNF ( ) 8 67 8 55 002

FCM fat-corrected milk SNF Solids-not-fat + P lt O1 P lt O05 P lt OOI

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b tb i 1L5 1 ~$(LI-yenf LfUij$IifffF-t 0

c bi61f~l ~ n t

2 yentplusmnl~

~j~I t1~))HM~A 3 (F L t IdiJPlt~a~ 0) ~~~Jffffi( l rt li t~ ltY) Gh tJ b t bi 8

Anim Sci I 70 ( 10) 390-396 1999 1392

fiiU4gt ) 7 jfO)7t~~$O)~ t) iJlpound4LLto) ~~ fcgt ct UI )vyen -1trnEdI-tJ5V

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f~~M~~iit~~tpound t~~~Jtamp1IlJ 771-1310 I tlillillj~t TA~flTm 818-0004g~~fJbf~~tJl

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(199935 ]tN 1999616 5[W)

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y 01m~[poundm li~~IJf plusmn~]ULt 2) ~1kthIHi ~flUlZiI Zi litJJgt-gttJi LDIXO)~

- 0) yenlJlitttii ~Lt J U~fil- 0) yen)f~~tflWJiplusmn~1JO9 QtfiioJ ( poundb I) PR - O)lJnllitl1 Ii Afl GJgt I ~ipoundt 9 QftfiioJ C poundb -gt t 3) xl Jv 4 - thR (i LD IX 0) ~-O) X j Jv 4 - fft ~w JiPP~ LiNial C ib -gt t Ji

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El ~~q~W 70 (10) J 390-J 396 1999

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iJlHfi1J IjJtfgtCf~ (fax 088-694-6211 e-mail nakaigreenpreLtokushimajp)

Anim Sci 1 70 ( 10) 1390-1396 1999 1390

Table 1 Ingredients and chemical composition of diets

Treatment Item

LD HD

Ingredients ( DM)

Roughage

Italian ryegrass wafer

Concentrate

Flaked corn

Barley grain

Beet pulp

Alfalfa meal

Soybean meal

Fish meal

Brewers grains

Soybean hulls

Calcium salts of fatty acids l)

Molasses

CaHP04

Mineral and vitamin supplements2)

Chemical composition ( DM or MJkgDM)

DM

CP

VIP ( CP)))

EE

NDF

GE

346

14 8 135

98

90 40

50

41 2 4

06

20

O 1

869

183 429

49

388 18 7

34 2

E6

02

~~ 6

8 1

12 6

7 2

15

19

O 9

O 1

868 18 2 32 3

4 7

39 1

185

LD low degradable protein HD high degradable protein DM dry matter CP crude protein VIP undegraded intake protein EE ether extracts NDF neutral detergent fiber

GE gross energy I) Megalac Volac Ltd

2) Trace minerals (contained 60 Zn 50 Mn 50 Fe 10 Cu and 01 I) vitamin A D E mixture (contained 10000 IVg vitaminA 2000 IVg vitaminD) and DL-a tocopherol acetate IOmgg) ) Calculated from Japanese Feeding Standard for Dairy Cattle (1994)

~JtJj 5 I k9 0) I JtJj 14 I ~R~ 2 WJ c L t I IX 2 lIfi 2 15lt i J I] CJ A 7f - - it I J i) ~Jlli Lt t1~Wl (I t~tj

ipound1LllI1M I r3) t t)1 A 5 l ftlipound~LLj 4 liJi -f m~ -frNJJJJllf lampU~9i~ir 7) 1lX1 L iji$ IO) i J i) yent

-7 17 I1f7t~itO)J tpound fiiiJf4-f~t L ~t J Lf x J U x jlt )1 -yen - WMiA~~ -f ktJili L t fJi~~f~ (I ~ Ut

)1 -yen - WW11 ifllJAE L t 18ce ftjxJitlJQ 60 c L t

lI fJ L t ipoundUJ 0) ijZ fJ 02 6lt tl 25 j[( (2 ~3 gf) -y fnJ u IJt~AftriJfO)Il)ampt J Lft~)amp7t I~ I I~ L t c t i)

~rrJIfcIfJO)ijZt-Jraquo~iampl3~il 49 13 (44~59 El) Cr3) Cr3)i) UIP~t7iltyentl pound11 11] lli (CP) Ix-tt t UIP O)~ IJ ~-f 43 lfJ~~ L t]X ( LD]Xc RI1lt) c 32

FiJ~~WIDIlampJtJj 7 I3t~~O)O)1 -fifnfiJ~iJtJj 9 l3 ~t $U ltMJ~ L t[R (HD ~ c~9 ) 0) 2 7iltyen c L t tpoundt ~F

Anim Sci J 70 ( 10) bull J390-J396 1999 J391

~~lffO)~~ It (i NRC ) ~~151 HD [RCIi UIP bq~

L0 Lt LJ -) ( LD IXCIi7H1t~7 1 Mb)~JE L

tJ ~ J -) (~)t It L t fii] 4 M5s- Ii tt ffrt L A+shy( ME ) AUI( ll) ~ftJE-t6ill~8 10 16tiJrJ 1911j0)

I Icl 4ltJIJt( tl~I=Jmiddot L t 7Jlt ti JlYIQl11~~IiEHl=tHUamp

~ +it

t1tfLil 8 Ill 3071 18 I~O) 2 J c L tf~WJ LHiH~

fL Lii0)5 1iRII cJt tlmiddotttyenf 0) fIamp ~n t ftJffO)~iJQ)jxJtiffimiddot$IO) rJI) rptJ3Tmiddotmiddot 5 -L r

flHtt (i T 7 - L r JttfTri lJ ) ( J I) 0tfT L t ~ti J

UI O)~ -- (iiiff fIfjoJ O) t t TV 7 - v$t J I) 0 tfT

L t fL)JX)1 (i 7HJ~ 13 gpJ0 tfTH (~vJ A f -V 134

A B Foss Electric 115 CntfT L t ~iJlIIi(i ftmNti

J U~( J L l liJfl~Iyen~ffir L 1Lti J CJPRI J ~

l r iJtlJ ~fi l l~~h t if I) L 7- v 7 -( v Lg~~PJ Ci6

ijZiX L6) ~liJf APi l4iiH (CA-4PI ~~~ftpJT) c0tfT L t

HUfx ME O) t ~Ixt -t 6fIJm~JJ$ (kp) (i6zAC j(ltY)

t

-ttJnt

kp = (1L L j v + - + ~fft L j v -+ - ) (ffiIXME - It J=ff(~9- 6 ME)

CftJ=ff I~-t 0 tt~ L v + - (i tt~f~ J) t I)

04866MI (J 163 kcal) 12) C L l~tplusmnl L t

tIt~HJ~IW SAS 0) GLM fo ~ -V _1 7) J I) 6z0)

-t- Tv ~ml l IT t

Y ijk =1 +ai + el +Bj+r +el

Yijk iJl11i T - 7

1 ttSj iS]

a U i O)lVJ~ Bj ftm4 j 0)gti7J~

Ik tU~AA k 0)gti1J~

el e2 I 6zblfUf ti J U 2 6z~~

t t ~~MO)5lt1J~i-)~l(i 2 6z~~~m~l F ~JE~

fi~ fQ~$SYXfO)~i5-(ifi~ampbiJ)0bO)c fG I~ ft 10 RfO) tFiiS-(itfil2ijb i J) 0 bO)cJtJ Lt

1 ~H~ll(~middotft~middot ftpx~

fiilD IJHJ1I ~~ 2 1 iF L t ijZjoJffiIIXk1 I -) ~l Ii LD IX (ti ~l li~rlSftmMrp

iJ5tc t - vtA~~ bi tllfiiiJ4tiJ Ulic-s-ft~NO)ffi

1lXtM r i- 0) J~W tHlltY) Gn -g fililIXfa9 t ~ Ii tJ b t

-1Lw I J l (iIIIJilX~a~ i N (i 17- Gn tJ b t bi -yenLfta H J ~l (j LD [2lt 0) 332 (xJ L HD IX 370 c tJ

Table 2 Effects of dietary protein degradability on milk production and composition

Treatment Item SE

LD HD

Body weight (kg) 599 593 3 14 DM intake (kgday) 19 3 193 049

Roughage 66 6 7 0 05 Concentrate 12 7 126 003

Milk yield (kg day) 349 348 O 19 4 FCM yield (kgday) 31 2 332 0 38 1

Milk

Fat () 3 32 3 70 006 Fat (kgday) 1 15 1 28 002+

Protein () 309 2 97 002 Protein (kgday) 108 104 0003 SNF ( ) 8 67 8 55 002

FCM fat-corrected milk SNF Solids-not-fat + P lt O1 P lt O05 P lt OOI

I) HD ~bifi~rM ~f~ctJt fL7 1~$ tiJ

U-1L7 ~1[ttsectlUMt-)~lli ~( LD Rbi HD R

lxtL i-n-fhO12 0 04kgfi ~( rEh~ f~~FL

t 0)6W FCM-1Lt liHD Rbi~lt tJ fI1i roJcJ)

I) ~fl5[QJJT-~0$C Ii LD Rbifi~ ( rEh ~ f~~F L t

~~M 5 0 1 IIi 0) 71 Mtt b i 1pound-1L ( ampIf -t 5lt1] W r -) ~

l Erdman and VandersalP) lijijoJtE7 -I 1 i[lI J I)

9 -I1 ~0M$~~z tftmM~M5s- L t c 0 119 0 1 [t$ (i 7t~ift1 0) ~ ~Q~ -4 0) M5s- C ~ lttJ I) ~L

~ ~Ua$Ii 9 1 ft7t~iftEr J I) ~~f~ secttlt tJ7J

t c L ltD rskov G16) lifx j[ -if l v - ~ f$O) ~I=J~~4

r~17f~~1JD L t c o ~1JDWIt6 L-1Lfi FCM ~L mbiampg 2 n 0 c L Spain and Polan l S

) (i ~ t) -t- o J ~

-if l v - tfO)~l=Jft~4r17f~ ~1JD-t 0 c C~U5

$ -yenLn allJJ t-tilJilbi i1iJX1gt-t 0 c L l ~0 L t 5lt1J5fi

0) sect (iftill 14114)amp 0) illy i UIP ~ 0) fiIlnt c Iit ti J U i- 0)

~ ( ~~ ~ n 0 b 0) c J5z Gn 0 bi ~~ 0) J -j (

UIP iLqic L lffi~Ifl~t~i5- - lErJ((lfLIi -yenL 5

OJ i1J$Ii~g2 n~U5fSliftff -t oflflJD] ( J) 0 c 2

n l ~0 5) 4~ (ti ~l b ~Lm O) ~1J[J Ii 17- G n tJ

b tb i 1L5 1 ~$(LI-yenf LfUij$IifffF-t 0

c bi61f~l ~ n t

2 yentplusmnl~

~j~I t1~))HM~A 3 (F L t IdiJPlt~a~ 0) ~~~Jffffi( l rt li t~ ltY) Gh tJ b t bi 8

Anim Sci I 70 ( 10) 390-396 1999 1392

Table 1 Ingredients and chemical composition of diets

Treatment Item

LD HD

Ingredients ( DM)

Roughage

Italian ryegrass wafer

Concentrate

Flaked corn

Barley grain

Beet pulp

Alfalfa meal

Soybean meal

Fish meal

Brewers grains

Soybean hulls

Calcium salts of fatty acids l)

Molasses

CaHP04

Mineral and vitamin supplements2)

Chemical composition ( DM or MJkgDM)

DM

CP

VIP ( CP)))

EE

NDF

GE

346

14 8 135

98

90 40

50

41 2 4

06

20

O 1

869

183 429

49

388 18 7

34 2

E6

02

~~ 6

8 1

12 6

7 2

15

19

O 9

O 1

868 18 2 32 3

4 7

39 1

185

LD low degradable protein HD high degradable protein DM dry matter CP crude protein VIP undegraded intake protein EE ether extracts NDF neutral detergent fiber

GE gross energy I) Megalac Volac Ltd

2) Trace minerals (contained 60 Zn 50 Mn 50 Fe 10 Cu and 01 I) vitamin A D E mixture (contained 10000 IVg vitaminA 2000 IVg vitaminD) and DL-a tocopherol acetate IOmgg) ) Calculated from Japanese Feeding Standard for Dairy Cattle (1994)

~JtJj 5 I k9 0) I JtJj 14 I ~R~ 2 WJ c L t I IX 2 lIfi 2 15lt i J I] CJ A 7f - - it I J i) ~Jlli Lt t1~Wl (I t~tj

ipound1LllI1M I r3) t t)1 A 5 l ftlipound~LLj 4 liJi -f m~ -frNJJJJllf lampU~9i~ir 7) 1lX1 L iji$ IO) i J i) yent

-7 17 I1f7t~itO)J tpound fiiiJf4-f~t L ~t J Lf x J U x jlt )1 -yen - WMiA~~ -f ktJili L t fJi~~f~ (I ~ Ut

)1 -yen - WW11 ifllJAE L t 18ce ftjxJitlJQ 60 c L t

lI fJ L t ipoundUJ 0) ijZ fJ 02 6lt tl 25 j[( (2 ~3 gf) -y fnJ u IJt~AftriJfO)Il)ampt J Lft~)amp7t I~ I I~ L t c t i)

~rrJIfcIfJO)ijZt-Jraquo~iampl3~il 49 13 (44~59 El) Cr3) Cr3)i) UIP~t7iltyentl pound11 11] lli (CP) Ix-tt t UIP O)~ IJ ~-f 43 lfJ~~ L t]X ( LD]Xc RI1lt) c 32

FiJ~~WIDIlampJtJj 7 I3t~~O)O)1 -fifnfiJ~iJtJj 9 l3 ~t $U ltMJ~ L t[R (HD ~ c~9 ) 0) 2 7iltyen c L t tpoundt ~F

Anim Sci J 70 ( 10) bull J390-J396 1999 J391

~~lffO)~~ It (i NRC ) ~~151 HD [RCIi UIP bq~

L0 Lt LJ -) ( LD IXCIi7H1t~7 1 Mb)~JE L

tJ ~ J -) (~)t It L t fii] 4 M5s- Ii tt ffrt L A+shy( ME ) AUI( ll) ~ftJE-t6ill~8 10 16tiJrJ 1911j0)

I Icl 4ltJIJt( tl~I=Jmiddot L t 7Jlt ti JlYIQl11~~IiEHl=tHUamp

~ +it

t1tfLil 8 Ill 3071 18 I~O) 2 J c L tf~WJ LHiH~

fL Lii0)5 1iRII cJt tlmiddotttyenf 0) fIamp ~n t ftJffO)~iJQ)jxJtiffimiddot$IO) rJI) rptJ3Tmiddotmiddot 5 -L r

flHtt (i T 7 - L r JttfTri lJ ) ( J I) 0tfT L t ~ti J

UI O)~ -- (iiiff fIfjoJ O) t t TV 7 - v$t J I) 0 tfT

L t fL)JX)1 (i 7HJ~ 13 gpJ0 tfTH (~vJ A f -V 134

A B Foss Electric 115 CntfT L t ~iJlIIi(i ftmNti

J U~( J L l liJfl~Iyen~ffir L 1Lti J CJPRI J ~

l r iJtlJ ~fi l l~~h t if I) L 7- v 7 -( v Lg~~PJ Ci6

ijZiX L6) ~liJf APi l4iiH (CA-4PI ~~~ftpJT) c0tfT L t

HUfx ME O) t ~Ixt -t 6fIJm~JJ$ (kp) (i6zAC j(ltY)

t

-ttJnt

kp = (1L L j v + - + ~fft L j v -+ - ) (ffiIXME - It J=ff(~9- 6 ME)

CftJ=ff I~-t 0 tt~ L v + - (i tt~f~ J) t I)

04866MI (J 163 kcal) 12) C L l~tplusmnl L t

tIt~HJ~IW SAS 0) GLM fo ~ -V _1 7) J I) 6z0)

-t- Tv ~ml l IT t

Y ijk =1 +ai + el +Bj+r +el

Yijk iJl11i T - 7

1 ttSj iS]

a U i O)lVJ~ Bj ftm4 j 0)gti7J~

Ik tU~AA k 0)gti1J~

el e2 I 6zblfUf ti J U 2 6z~~

t t ~~MO)5lt1J~i-)~l(i 2 6z~~~m~l F ~JE~

fi~ fQ~$SYXfO)~i5-(ifi~ampbiJ)0bO)c fG I~ ft 10 RfO) tFiiS-(itfil2ijb i J) 0 bO)cJtJ Lt

1 ~H~ll(~middotft~middot ftpx~

fiilD IJHJ1I ~~ 2 1 iF L t ijZjoJffiIIXk1 I -) ~l Ii LD IX (ti ~l li~rlSftmMrp

iJ5tc t - vtA~~ bi tllfiiiJ4tiJ Ulic-s-ft~NO)ffi

1lXtM r i- 0) J~W tHlltY) Gn -g fililIXfa9 t ~ Ii tJ b t

-1Lw I J l (iIIIJilX~a~ i N (i 17- Gn tJ b t bi -yenLfta H J ~l (j LD [2lt 0) 332 (xJ L HD IX 370 c tJ

Table 2 Effects of dietary protein degradability on milk production and composition

Treatment Item SE

LD HD

Body weight (kg) 599 593 3 14 DM intake (kgday) 19 3 193 049

Roughage 66 6 7 0 05 Concentrate 12 7 126 003

Milk yield (kg day) 349 348 O 19 4 FCM yield (kgday) 31 2 332 0 38 1

Milk

Fat () 3 32 3 70 006 Fat (kgday) 1 15 1 28 002+

Protein () 309 2 97 002 Protein (kgday) 108 104 0003 SNF ( ) 8 67 8 55 002

FCM fat-corrected milk SNF Solids-not-fat + P lt O1 P lt O05 P lt OOI

I) HD ~bifi~rM ~f~ctJt fL7 1~$ tiJ

U-1L7 ~1[ttsectlUMt-)~lli ~( LD Rbi HD R

lxtL i-n-fhO12 0 04kgfi ~( rEh~ f~~FL

t 0)6W FCM-1Lt liHD Rbi~lt tJ fI1i roJcJ)

I) ~fl5[QJJT-~0$C Ii LD Rbifi~ ( rEh ~ f~~F L t

~~M 5 0 1 IIi 0) 71 Mtt b i 1pound-1L ( ampIf -t 5lt1] W r -) ~

l Erdman and VandersalP) lijijoJtE7 -I 1 i[lI J I)

9 -I1 ~0M$~~z tftmM~M5s- L t c 0 119 0 1 [t$ (i 7t~ift1 0) ~ ~Q~ -4 0) M5s- C ~ lttJ I) ~L

~ ~Ua$Ii 9 1 ft7t~iftEr J I) ~~f~ secttlt tJ7J

t c L ltD rskov G16) lifx j[ -if l v - ~ f$O) ~I=J~~4

r~17f~~1JD L t c o ~1JDWIt6 L-1Lfi FCM ~L mbiampg 2 n 0 c L Spain and Polan l S

) (i ~ t) -t- o J ~

-if l v - tfO)~l=Jft~4r17f~ ~1JD-t 0 c C~U5

$ -yenLn allJJ t-tilJilbi i1iJX1gt-t 0 c L l ~0 L t 5lt1J5fi

0) sect (iftill 14114)amp 0) illy i UIP ~ 0) fiIlnt c Iit ti J U i- 0)

~ ( ~~ ~ n 0 b 0) c J5z Gn 0 bi ~~ 0) J -j (

UIP iLqic L lffi~Ifl~t~i5- - lErJ((lfLIi -yenL 5

OJ i1J$Ii~g2 n~U5fSliftff -t oflflJD] ( J) 0 c 2

n l ~0 5) 4~ (ti ~l b ~Lm O) ~1J[J Ii 17- G n tJ

b tb i 1L5 1 ~$(LI-yenf LfUij$IifffF-t 0

c bi61f~l ~ n t

2 yentplusmnl~

~j~I t1~))HM~A 3 (F L t IdiJPlt~a~ 0) ~~~Jffffi( l rt li t~ ltY) Gh tJ b t bi 8

Anim Sci I 70 ( 10) 390-396 1999 1392

~~lffO)~~ It (i NRC ) ~~151 HD [RCIi UIP bq~

L0 Lt LJ -) ( LD IXCIi7H1t~7 1 Mb)~JE L

tJ ~ J -) (~)t It L t fii] 4 M5s- Ii tt ffrt L A+shy( ME ) AUI( ll) ~ftJE-t6ill~8 10 16tiJrJ 1911j0)

I Icl 4ltJIJt( tl~I=Jmiddot L t 7Jlt ti JlYIQl11~~IiEHl=tHUamp

~ +it

t1tfLil 8 Ill 3071 18 I~O) 2 J c L tf~WJ LHiH~

fL Lii0)5 1iRII cJt tlmiddotttyenf 0) fIamp ~n t ftJffO)~iJQ)jxJtiffimiddot$IO) rJI) rptJ3Tmiddotmiddot 5 -L r

flHtt (i T 7 - L r JttfTri lJ ) ( J I) 0tfT L t ~ti J

UI O)~ -- (iiiff fIfjoJ O) t t TV 7 - v$t J I) 0 tfT

L t fL)JX)1 (i 7HJ~ 13 gpJ0 tfTH (~vJ A f -V 134

A B Foss Electric 115 CntfT L t ~iJlIIi(i ftmNti

J U~( J L l liJfl~Iyen~ffir L 1Lti J CJPRI J ~

l r iJtlJ ~fi l l~~h t if I) L 7- v 7 -( v Lg~~PJ Ci6

ijZiX L6) ~liJf APi l4iiH (CA-4PI ~~~ftpJT) c0tfT L t

HUfx ME O) t ~Ixt -t 6fIJm~JJ$ (kp) (i6zAC j(ltY)

t

-ttJnt

kp = (1L L j v + - + ~fft L j v -+ - ) (ffiIXME - It J=ff(~9- 6 ME)

CftJ=ff I~-t 0 tt~ L v + - (i tt~f~ J) t I)

04866MI (J 163 kcal) 12) C L l~tplusmnl L t

tIt~HJ~IW SAS 0) GLM fo ~ -V _1 7) J I) 6z0)

-t- Tv ~ml l IT t

Y ijk =1 +ai + el +Bj+r +el

Yijk iJl11i T - 7

1 ttSj iS]

a U i O)lVJ~ Bj ftm4 j 0)gti7J~

Ik tU~AA k 0)gti1J~

el e2 I 6zblfUf ti J U 2 6z~~

t t ~~MO)5lt1J~i-)~l(i 2 6z~~~m~l F ~JE~

fi~ fQ~$SYXfO)~i5-(ifi~ampbiJ)0bO)c fG I~ ft 10 RfO) tFiiS-(itfil2ijb i J) 0 bO)cJtJ Lt

1 ~H~ll(~middotft~middot ftpx~

fiilD IJHJ1I ~~ 2 1 iF L t ijZjoJffiIIXk1 I -) ~l Ii LD IX (ti ~l li~rlSftmMrp

iJ5tc t - vtA~~ bi tllfiiiJ4tiJ Ulic-s-ft~NO)ffi

1lXtM r i- 0) J~W tHlltY) Gn -g fililIXfa9 t ~ Ii tJ b t

-1Lw I J l (iIIIJilX~a~ i N (i 17- Gn tJ b t bi -yenLfta H J ~l (j LD [2lt 0) 332 (xJ L HD IX 370 c tJ

Table 2 Effects of dietary protein degradability on milk production and composition

Treatment Item SE

LD HD

Body weight (kg) 599 593 3 14 DM intake (kgday) 19 3 193 049

Roughage 66 6 7 0 05 Concentrate 12 7 126 003

Milk yield (kg day) 349 348 O 19 4 FCM yield (kgday) 31 2 332 0 38 1

Milk

Fat () 3 32 3 70 006 Fat (kgday) 1 15 1 28 002+

Protein () 309 2 97 002 Protein (kgday) 108 104 0003 SNF ( ) 8 67 8 55 002

FCM fat-corrected milk SNF Solids-not-fat + P lt O1 P lt O05 P lt OOI

I) HD ~bifi~rM ~f~ctJt fL7 1~$ tiJ

U-1L7 ~1[ttsectlUMt-)~lli ~( LD Rbi HD R

lxtL i-n-fhO12 0 04kgfi ~( rEh~ f~~FL

t 0)6W FCM-1Lt liHD Rbi~lt tJ fI1i roJcJ)

I) ~fl5[QJJT-~0$C Ii LD Rbifi~ ( rEh ~ f~~F L t

~~M 5 0 1 IIi 0) 71 Mtt b i 1pound-1L ( ampIf -t 5lt1] W r -) ~

l Erdman and VandersalP) lijijoJtE7 -I 1 i[lI J I)

9 -I1 ~0M$~~z tftmM~M5s- L t c 0 119 0 1 [t$ (i 7t~ift1 0) ~ ~Q~ -4 0) M5s- C ~ lttJ I) ~L

~ ~Ua$Ii 9 1 ft7t~iftEr J I) ~~f~ secttlt tJ7J

t c L ltD rskov G16) lifx j[ -if l v - ~ f$O) ~I=J~~4

r~17f~~1JD L t c o ~1JDWIt6 L-1Lfi FCM ~L mbiampg 2 n 0 c L Spain and Polan l S

) (i ~ t) -t- o J ~

-if l v - tfO)~l=Jft~4r17f~ ~1JD-t 0 c C~U5

$ -yenLn allJJ t-tilJilbi i1iJX1gt-t 0 c L l ~0 L t 5lt1J5fi

0) sect (iftill 14114)amp 0) illy i UIP ~ 0) fiIlnt c Iit ti J U i- 0)

~ ( ~~ ~ n 0 b 0) c J5z Gn 0 bi ~~ 0) J -j (

UIP iLqic L lffi~Ifl~t~i5- - lErJ((lfLIi -yenL 5

OJ i1J$Ii~g2 n~U5fSliftff -t oflflJD] ( J) 0 c 2

n l ~0 5) 4~ (ti ~l b ~Lm O) ~1J[J Ii 17- G n tJ

b tb i 1L5 1 ~$(LI-yenf LfUij$IifffF-t 0

c bi61f~l ~ n t

2 yentplusmnl~

~j~I t1~))HM~A 3 (F L t IdiJPlt~a~ 0) ~~~Jffffi( l rt li t~ ltY) Gh tJ b t bi 8

Anim Sci I 70 ( 10) 390-396 1999 1392

Table 3 Effects of protein degradability on

nitrogen utilization

Treatment Item SE

LD HD

Intake nitrogen (g day) 566 1 5623 2 44 Feces 196 4 192 3 077

Urine 1598 1755 3 36 gt

Milk 1712 162 4 1 51 gt

R etention 38 7 321 125+ Milk Retention 209 9 194 5 231

Ratio for intake nitrogen ( )

Feces 347 34 2 O 06 Urine 28 3 312 O 49+ Milk 302 28 9 O 30+ Retention 68 5 7 022+

+ P lt01 P lt 005

fi~ Nmit~1Y) 0) CP ~iH )(t L -C -C 0) Clpound$ (j LD IX 111 HDR5107Tib IJ gtgtiIampIIT ib-gt t ~ lfJ-

0) ~~tmmiddot[ltm ~Lcpf3 J Cyt ipoundHlll- 0) ~ftfJ (j LD

IXIJ HD lgtU~ltr-c$ lt tJ QfiIo1Tib -gt t ~ t J7K rj1

- 0) ~~tFiltt~ (j LD IZ~ t ~ I -C (j Rjj IJgt I~ iliipoundj 9 Qtfi

IolTib -gt t LD 1Z(j HD IZ (~M L~~HHampST 38 g

$ ~ I IJ Ii i~mO) 41 g IJ~CP (~tFTtt 2 n fLifl e ~

fi(~ bK[thO)tFiill~~O) ~jgtZe lili[ci]mO) 154g IJ

ftrr L t L e (~tJ Q lJnamp3[O) -) t ~L cp e t~fft(~

ftrr L t ~~~1i1f~~1Il e 9 Q e LD IZ (j 2099 g

HD 1Z(j 1945 g e tJ V) jilijlZr89(~fl~~IJ~p66 ntlJi

1~~middott1U xi9 Q ncpftrr~iO)~I~ (j LD IZ

82 HD J3 83 Tib V) ~ (j~P66 ntJIJgt -gt t

HOfx~(~)i9 QII~camp Q e bKcp-O)~~~h11

~ (j HD IZIJ$ lt ~Lt J Ut~fft-O)~ftrrll~ (j

LDlZffi$ lt~Q tfiIolT ib-gtkbullcp-O)~~mTtt~~

T(jjilij~rB9(~flzifIJ~p66 ntlJi -C0)~ (j 05 (~

jgJ trc tJ IJgt -gt t 17K I~J - 0) J1~ i1+J II ~ (j iiLj IX 0) yen- il Ml c 29 8 cib V) L O)f~(l~FR 20) m 14) O) f(~Jtt L

-cAjj 1Jgt(~~~lftjIolCJ) -gt t ~ t ~Efl G20) (j ilsectiJliI

lit o) CP ~J-lJi ~LjJ-O)yenJ1rrI~ w ~tfllJll2 tt Q L e ~

t~lm L -Cf3 V) NtfR(~Jtr-cbKLj 1~~tJ=illtlliIJ~IJgt -gt t

L e b~~~O) CP ~qiW ( ~joJLll 182~183 ) IJ

R (~ N L JgtjfampJliITf) t L e ~t-jlt -c ~ I Q er

z nt

j)J0)J -) (~ 1~~To) CP fV=jmiddotililJjJliITib -gt t

L e lJjtttto) fR1 1421J e ltr UIP ~~(~ J Q iktiJillt

lMtfIlfull XIJl IJqi~I - IJt ~ I JMfAI e ~z n t L IJgt L CP

jJljiI~q~nTT ib -gt -C b 7 u 85J~fffO)~~ ij

~0)fLl1J-O)ftfJttl e UKrp-O)J1Illt1l (~Bjj G IJgt (~~~~

-g Q L e IJql(W 2 n t 3 I- Ji-=F- tB~

I )vyen - W~IlXt~poundZ 4 (~~ L t ftMi(fJ) tJ O)$t Ijgt )v-t- - (GE) iJIiPlft I jgt)v

yen- (DE) MEffiIampjH~amp (Hp66Gn1 B~ii]~~

~12 ) (~)i 9 Q ME -Jf $ (j LD IR IJ 107 HD J3 lJi

103 Tit -gt t

~~ (~f3lt Q~t I jgt )v yen - 0) jcentlf tyen (DE GE) f3 J

CY~Mf~ (MEGE) 1middotjIJijJ3O)yen-~JllT-C nfn 725

f3 J U 645 Tit fJ ~tEjt GS) 0) 636f3 J U 552

~ JJiZ G 0) 650 f3 J ( 566 e 1trlJgt tJ IJ ~ l 1ij1 T

ib -gt t I t )v yen - rn~11 r~ -J ~ I -C kt LD ~ 0) ji cp - 0) I j

)v-t- -~FrIkMIJ~gt~~ lttJ Ql3jIolTib -gt tlJi J7Kf3 J U

j e L -C 0) I jgt )v yen - m~- tJ G ( (~1)E1m (~amp

IHr66 GntJIJ -gt t tff(~xJ-g Q Ijgt )vyenmiddot-Imiddot~-J l-C

(j fLO) Ijgt)v -t- - Itt Ii LD IZIJgt gtjgttJl tJl1ol T ib

V) I jgt )v-t - ~MITUi LD IRIJflCd~~ lt tJ Qtjr6JT

it -gt t ME (~)J9 Q ~L It)v yen - L ~f1 I jgt )v -t- - (LE +

RE) O)till~ Ij LD [g 509 HD IZ 508 T ib V)amp (Hr 66GntJlJ-gtt ~t LD lZe HD[gO) ME O) 1i1f(~

)(i9 Q fIJffl~fJyen kp Ii -C hfh 0679 0681 e tJ IJ fl(j

~r66tltJIJ -gtt LO)~(Ht~rnlG8)0) 58 Moe G9)

0) 62 J V) b ~HtjiroTc ib -gt t IJ Ijgt)v yen - fIJffl ~fJ

$~~q~O)~~yenffiJffiQ~~~ lt~Qe2 n-ct

V) gt ~~O) ME ~)i9 Q LE+ RE 0) t11~f3 JCf ME

0)Ipoundi1f (~)i 9 Qfljfl~h5f]qlHtO)W~489 ) e 1t L -C riJ

IJ -gt t L e (j r~~middotftnlifO) trJflf e n~JUi 2zO) riJ 2 ~ rx L t bO) e~z Gn t

~)JiZSrt J -) (~ X~T (j It )v yen -f3 J U~~

8J~(j ~ 1ft b fflql~ ~~ L -Cf3 I) jOt(~ -J ~ -C (H F ilamp iIll T J) -gt t b 0) e 1llitIT 2 ~1 t L IJ L IILjIx ~~1 T UK I~ I

I t )vyen - ti l i llt ttl~ (j ( j tr66 nf fiiilN f1I 0) 71~t tI 0) ji l I Ij -1X ~~ lUQ o)amp ~ I T ib n (i I jgt )v

yen - ilHT(middot~ iH~~~~ Xli 2 tJ ~I b 0) e fcEh n t ~

t Tyrrell 2) (jRJJ0l 0)~lf~~ffiIlii L ttil~ iot -1L4TI j I g illi~Ig~~ HUampibt V) ME T 72 kcal O)m~

(~ -J ti tJi Q e L -C f3 V) -C 0) ~ HI ~ I -C Iill] IX 0) I jgt )v yen - til )( ~i) ~ ~JJ~ 9 Q e (11 -il iHilif-12) 0) C P ~)lt lli

(~Jtt L L~~ e L -C LD IX T 554 g HDXT 345 g

ilampJliD 1 Clef)tV) LD ~ I7Ml HD IZ lOMJ -CO)j[

Anim Sci 170 (10) 390-396 1999 1393

Table 4 Effects of dietary protein degradability on energy utilization

Treatment Item SE

LD HD

Intake energy (MJday)

GE 361 7 357 7 1 51 DE 261 3 2606 069 ME 233 2 2306 099

Energy loss (kJkgo75day)

Feces 8328 812 3 4 71+

Urine 68 0 733 2 30 Methane 165 0 178 3 546 Heat production 9480 9480 2 46

Energy of produc tion (kJkg0 75 day)

Milk 805 5 8408 7 73+

Energy retention 1808 141 5 5 64 Ratio for intake GE ()

Feces 278 27 1 O 13 ~

Urine 2 3 2 4 007 Methane 55 60 017 H ea t production 31 6 31 7 005 Milk 268 281 030+ Retention 60 4 7 016

DEGE () 722 72 9 013 ME GE () 64 6 64 5 O 20 EB ME intake () 509 508 O 16 Efficiency of ME utilization for production (kp) 0679 0681 0008

GE gross energy DE digestible energy ME metabolizable energy EB LE+RE milk energy +

retaind energy MEm 04866 MJ metabolic body size kp Efficiency of utilization of ME was

derived from equation of kp=EB(ME intake-MEm)

+ P lt O1 p lt om

Ij 07 MJ day c tJ I) I )vofmiddot - OYfIJm~JJ (jli c

Amiddotv~~i-poundj JH liUjl c it -gt t 1 t tiltrtA~FTtll fil O)

l~hIIHf1Wc L nJ~-g Q C jQ( HD IxO)m14itJi

LD ~J I~ b 07 MJ day ~ ~ C C ( tJ Q tJ i ~Th bI )V-yen-fiJm ~M~( 2 lt(H~i~LtJ ~b o)c~hh t

jLto) c c tJ G Jl1lt1~]yen-t3Intt~ (j ft~NiJ O) -7 -lt

1 Pi5i~yen$i- li ltJ1tl2 Z Q c c I J -gt n[j1il~ll2 h QtJi -C O)gt(hm(j CP 7Ilti$tJi og) ~(i c fJ1Tq- Q c c 1 t lt$

x~0)~14Ti=B~ -r(j -7 ~1n7tW(fO)jily (~gj(

ItJI t )V -yen - t3 1 TlIJ~ - I t )vofmiddot - f il m ~YJfl (jll~~ i- amp Ii tH c C tJi nJjGtJc tJ -gt t L tJ L yenk~~(j 7 -shy~1iq tJi-- iJZl~lcmiddot(~it QtJi I)Vof- -7 ~1it c

Anim Sci J 70 ( LO) 390-396 L999 1394

b 1Cfi h t tI iRT c 6 -gt -r B I) OLdham 15) 0) mm I bit Q J -) I I 1 )v -yen -B J U -7 -- ~ 1 Jf O)fA~poundilttJ if L lt~ tJ Q~ffTc I~I 1 )V -yen - fIJJfjgt()J$tJf1l1~jIJ it Q

H~~-5-2hQ c c bl5Z GhQ c ctJG 2 Gampfi ~ltjTc O)oxtt i- ~fl-g Qplusmn~tJi it Q b 0) c 15 Z Gh

lt$~~~ i- msfr-g Q I it t I) ~~niJrlJ 0) fiiJ ifeBf~IH ~ -jtJ CW) i- ~t tmiddot~ b~jiQrjfi Yr a~~ijjtfElliJ1il+

giUUf I HO)~N~ 1t+5itJTi-m~ L-r~tt~t~U

~~ijjfiri1JXtl~Ciyenit~0fftyen 1IUJ~1~+L ~o~~ al~ lfI~ t L19

I) Agricultural Research Council The Nutrient Requireshy

ments of Ruminant Livestock 2nd ed 78-81 Commonshy

wealth Agricultural Bureaux London 1980

2) Coppock CEo Energy nutrition and metabolism of the

lactating dairy cow Journal af Dairy Science 68 3403shy

34 10 1985

3) Erdman RA Vandersall JH Effect of rumen protein

degradability on milk yield of dairy cows in early lactation

Journal of Dairy Science 66 1873-1 880 1983

4) ~~~flse rn~iiL~I~ Wyen mIItl Iv~ 11 WiifL

t o) r Iv4middot -K~(rt B~ Epoundyenc 0W 66 374-382

1995

5) Hussein HS Jorda_n RM Fish meal as a protein supplement

in ruminant diets A review Journal of Animal Science 69

2147-2156 1991

6) fjl~ nmiddot IfImrIii~i lIJJI$tiIJ~iJ)idt~(~c L [f I) r

Tv 7 1 )v L ~ IfJ l t ~jit to J cf DIlt O)tl Hfr fE

O)fi ~i iampt~J~bJfJtW 32 39-43 1977

7) ElifIiifDMpound HiJi12i 1fIJl1fBUtti ~IfIX J)l (fI~ ~

it IJi] lrg-middot - ~nii~~ (ffi~) L t ~tt~~4iiil-J

I[ - C lt 11lf10ttA~4iiITO)~Ii~~q Lc L [ - fdE ca ~~frJf1fQ 39 41-7 3 1982

8) ~rjjbtmmiddot ~ mii1l tElj fft ~~~ffi- ii~LO)r

to Iv 4 - f-tiAt I amp (J 9 m~iMcJj[ O)W~~- t 1 v - to J cfrz~ lsect Il1f- BEeuro~~yenR 63 831-839 1992

9) Moe PW Tyrrell HF Hooven NMJr Energy balance

mesurements with corn meal and ground oats for lac tating

cow Journal of Dairy Science 56 1149-1153 1973

10) ~ 7i~f~ ilJ~fttJ~$ ~ I rJJi 191-257 280

-298 D~pound liD 1971

II ) National Research Council Nutrient Requirements of

Dairy Cattle 6th revi sed 10-20 National Academy Press

Washington DC 1988

12) J~Hf Jlt rlf ft tHfr ~ amp 1Hfi fJ ~ J Hi) H~ ~ ~L t ( 1994 l~J) 4J9~fiJir0 WJ~ 1994

13) imH7llttf 1i -$ iiI U~tH~ $If l l I fi~ h j[fr u -t- 0) leo J-Ij i l(ii~~t~riftNo 56-I 1981

I 4) m 2I Iii iIrj J)Hi IHi fHui J 1F~ i~ 8j n tJ) millt fll to J u ttfj( middot-tt (O)([ljiX ~iJIfII6)fJc~ru 42 155shy

165 1998

15) Oldham JD Protein-Energy Interre lationships in dairy

cows Journal of Dairy Science 67 bull 1090- 1114 1984

16) ltDrskov ER Reid GW Tait CAG Effect of fish meal on

the mobilization o f body energmiddot in dairy cows Animal

Production 45 345-348 1987

17 ) SAS SAS STA T z - -If - A tJ ~ 603 I ~i 569-666

SAS tfj rJJifi m J~ 1990

18) Spain IN Polan CE Watkins BA E valu a ting effects of fish

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Science 78 1142-1153 1995

19) Tamminga S Nutrition managemen t of dairy cows as a

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~ ~rn~~middot ~~~mmiddot~ rn~~middot ~~ ~ ~~~Kto H i$ ~riIf Ul O)tftlE BiiiIlT 2W 68 163-168

1997

21) cf EB )( I igfr ~ iNLit O)t7F- fJFnltr(ampi9fttJ))

~-~ C m1lhlihl1t 0)~~~~ II ~ffi~0W 69 620-624

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22 ) ~ EE~tUl IlnJ gf~Z ~Em~L iUliE~ 1I11~tO)t

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Anim Sci J 70 (10 ) 390-396 1999 1395

middot JJR fv J c 7 -( iiili

Effects of Dietary Protein DegradabilUy on litrogen

and Energy Utilization of Lactating

Fuminori Yoshinori Koichiro

HIGUCHI Osamu ENISHlo and Fuminori

Tokushima Prefectural Livestock Experiment Station Kamiita-machi Tokushima-ken 771-1310 Japan I Fukuoka Agricultural Research Center Chikushino-shi 818-0004 Japan

1 Faculty of Agriculture Hokkaido University Kita-ku Sapporomiddotsht 060-8589 Japan National Institute of Animal Industry Tsukuba Norin Kenkyu Danchi Ibaraki-ken 305-0901 Japan

Corresponding Fllminori NAKAI (fax +81 (0) 88-694-6211 e-mail ntrillmO~M

To determine the effects of undegraded intake (UIP) on the energy and nitrogen u tiliza tionbalance

trials were carried out with four cows The trials were conducted an open circuit

apparatus at SoC and 60 relative humidity The cows were fed on diet fish meal and brewers grain

43 UIP in CP) or soybean meal according to cross over I) The results showed no difference

(P gt005) in milk between the diets The diet fish meal and brewers grain increased the milk

but decreased milk fat percentage to the diet containing soybean meal 2) There was no difference in N intake

between the diets however LD increased the N excretion in feces N in milk and retained N of cows and decreased the

N excretion in urine to the HD 3) Energy loss in feces of the cows fed LD was than that of HD

loss in urine methane and energy metabolizability values were ahnost similar between the diets

retained in the body increased (P when cows consumed LD 4) The efficiency of utilization of

metabolizable energy for milk energy and retention energy +RE) was 68 and no difference was observed

between the diets The results of this showed that the milk content and N balance was influenced by VIP

but not the energy metabolizability and of utilization of ME for LE +RE

Animal Science Journal 70 (10) J390-J396

Key words cows Protein degradability Nitrogen balance Energy balance

Anim Sci J 70 (10) 390-396 1999 J396