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Analysis of Phenotypic Data Using PM Software
File ManagementParametric Analysis
v. 1.2.02
Michael Ziman, Ph.D.
Director, PM Services
Overview
• File Management Version/Replicate Analysis– Organize OmniLog data– View replicate and average graphs– Export raw data replicates
• Parametric Version/Pairwise Comparison– View replicate and average graphs– Create Pairwise Comparison– Export average parameters
File Management/Kinetic Plot
Data Organization: by assay or filter
PM1.oka PM11.oka
PM2.oka PM12.oka
PM3.oka PM13.oka
PM4.oka PM14.oka
PM5.oka PM15.oka
PM6.oka PM16.oka
PM7.oka PM17.oka
PM8.oka PM18.oka
PM9.oka PM19.oka
PM10.oka PM20.oka
PM1.oka PM11.oka
PM2.oka PM12.oka
PM3.oka PM13.oka
PM4.oka PM14.oka
PM5.oka PM15.oka
PM6.oka PM16.oka
PM7.oka PM17.oka
PM8.oka PM18.oka
PM9.oka PM19.oka
PM10.oka PM20.oka
File Management
Reproducibility Analysis Flowchart
Average Replicates
Display and Analyze Reproducibility
Selected Files in Data List
Reproducibility Analysis
Export Kinetic Data
Raw Data Replicates
PM1.oka PM11.oka
PM2.oka PM12.oka
PM3.oka PM13.oka
PM4.oka PM14.oka
PM5.oka PM15.oka
PM6.oka PM16.oka
PM7.oka PM17.oka
PM8.oka PM18.oka
PM9.oka PM19.oka
PM10.oka PM20.oka
PM1.oka PM11.oka
PM2.oka PM12.oka
PM3.oka PM13.oka
PM4.oka PM14.oka
PM5.oka PM15.oka
PM6.oka PM16.oka
PM7.oka PM17.oka
PM8.oka PM18.oka
PM9.oka PM19.oka
PM10.oka PM20.oka
MUT.dlb
WT.dlb
File Management
Parametric Analysis
Pairwise
Comparison
Pairwise Comparison Flowchart
Parametric Version: Load Data List(s)
Calculate Parameters
0
50
100
150
200
250
300
350
1 21 41 61 81 101 121 141 161 181
Time (Hour)
Om
niL
og
Sig
na
l
0
10
20
30
40
50
60
70
80
90
100
Slo
pe
a-D-Glucose1st Derivative
Slope
Maximum Signal
Time to Maximum Slope
Lag
Average Height = sum of reads divided by number of reads
dXdY
TEST vs REFERENCE~2000 Kinetic Curves
Defects in Tryptophan Metabolism
Report
Export Parameters
Average Parameters or Paramter Difference Values