Ancient Lake

non-isolated pothole

River where pupfish can swim from pothole to

pothole.

isolated potholes

Hypothesis is that there was an ancient lake filling a

basin.

Current Lake

The lake dried up leaving potholes where pupfish live

now. Isolated potholes do not connect and non

isolated potholes are connected by rivers.

PCRPolymerase Chain Reaction

• Allows us to amplify a particular segment of DNA- over and over.

• Forensics- amplify tiny amount until large enough to use.

Ingrediants

• Template DNA• Taq Polymerase• Primers• dNTPs• Buffer• Magnesium Ions

http://www.youtube.com/watch?v=2KoLnIwoZKU