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A N J A L I V. SHAH LinkedIn- AnjaliVShah | 408-693-8914 | [email protected] |
SUMMARY Blends theoretical knowledge with intensive research training at the Mayo Clinic’s SURF Program to offer solid skills in diverse
laboratory techniques ranging from neuro-genetics, cell biology, microbiology and organic chemistry.
EDUCATION RUTGERS UNIVERSITY, NEW BRUNSWICK, NJ
B.A in Cell Biology and Neuroscience Minors in Psychology and Cognitive Science Graduating Class of May 2015, Deans List
R E S E A R C H EXPERIENCE SUMMER UNDERGRADUATE RESEARCH FELLOWSHIP,
MAYO CLINIC, JACKSONVILLE, FL MAY 2013 -AUGUST 2013
• Narrowed down a list of 1200 genetic variants to 2 rare variants that could cause Essential Tremor using Exome Sequencing, Sanger Sequencing and TaqMan Genotyping.
• Used techniques such as PCR and Gel Electrophoresis.
• Operated the BioMek FX automation machine and the Life Technologies 3730 Sequencer.
• Analyzed and compared variants in the wild type population to those diagnosed with Essential Tremor using SeqScape software.
M AY O C L I N I C R E S E A R C H T H E S I S PRESENTATION Familial Essential Tremor Gene Identification using Exome Sequencing.
Exome sequencing of a family with familial Essential Tremor (ET) revealed 2 ET gene carriers who shared 1200 SNPs/variants. Only 70 were non-synonymous, which were further narrowed down to 18 variants segregating within the affected members of the family. The 18 were followed up in controls to see whether they were rare, leaving 3 rare variants. Using Sanger sequencing and TaqMan Genotpying assay, I ultimately isolated 2 rare variants seen in other ET cases.
SKILLS
Neuro-Genetics Laboratory, Mayo Clinic (May 2013 – August 2013)
Performing PCR and Gel Electrophoresis procedures. Using FluroChem SP gel imaging software. Proficient in the Ampure Clean-Up procedure, Cycle Sequence Reaction and Cycle Sequence Clean-Up procedure using the BioMek FX automation machine. Proficient in using the Life Technologies 3730 Sequencer to get sequencing data and then using software such as SeqScape to analyze the sequenced data.
Microbiology Laboratory, Rutgers University (January 2014 – May 2014)
Experimental Design, Microscopy, Plating and incubation of Microbial cultures, Differential Staining- The Gram Stain, Serial Dilutions, Isolation of bacterial species from mixed culture using the Quadrant Streak Method, Plasmid Isolation, Transfection and Gel Electrophoresis.
Cell Biology Laboratory, Rutgers University (January 2015 – May 2015)
Working with bacterial and mammalian cell cultures. Using restriction enzymes and GFP. Plasmid isolation using QIAprep Spin Miniprep Kit. Transfection/Transformation, 2D Gel Electrophoresis, SDS-PAGE, Primer Design, PCR, ELISA assay and Western Blot.
Organic Chemistry Laboratory, Rutgers University (January 2015 – May 2015)
Working with MelTemp apparatus. experimental design, running reactions, product isolation, purification using various techniques- recrystallization, distillation (simple, steam and vacuum), acid-base extractions, Thin Layer Chromatography, Column Chromatography and product identification using Infrared Spectroscopy.
Technical
Proficient in Scratch programming, MS Office, Windows and Mac OS, Prezi and Adobe Software.
Science Communication and Writing
Active blogger - http://anjalivshah.wix.com/futureneuroscientist
Languages
Fluent in English, Hindi and Gujarati
