antimicrobial surfaces

7/29/2019 antimicrobial surfaces

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About us Our experience in nanotechnology

Synthesis methods

Lab for synthesis & characterization

Projects

Publications

Participants

Antibacterial surfaces Conclusions

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A materials research group that belongs to the Faculty ofScience, Universidad Autnoma de San Luis Potos.

Our current research interests are in the field of

nanotechnology. These research interests have emerged

according to the projects we have been developing in this

field.

All these interests are related to the synthesis,characterization and applications of nanoparticles.

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-NPs antibacterial properties

- Cytotoxicology of NP`s

- Colloidal dispersions

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- - Dynamic of nanoparticles(Growth and forming studies)

- - Large scale productions

methods


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Formally we started our work in the area of

nanotechnology in 2004 exploring different

methods for the synthesis of metal nanoparticles.

In particular, this project was started at therequest of one of the largest mining companies in

Mexico. In the first stage we explored different

methods of synthesis at the laboratory for four

different materials, silver, gold, zinc oxide, andmagnesium hydroxide.

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Zn

ZnO or Zn(OH)2

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Titanium oxide(TiO2)

Silver

Gold

Copper

Platinum

Zinc oxide

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Alongside with the methods of synthesis for

nanoparticles we develop. We have

implemented a methodology for nanoparticle

characterization. This sounds like somethingobvious but it is necessary to have a

documented methodology for a long term

projects or when you develop methods for

others.

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300 350 400 450 500 550 600 650

-0.4

0.0

0.4

0.8

1.2

1.6

2.0

2.4 max433 nm

Absorbanc

e

Longitud de onda (nm)

H

L

N

P

G

I

K

M

O

12 horas

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Silver


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0 50 100 150 200 250 300

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13

CDB

TGA

Temperatura (C)

Peso(%)

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-8

-6

-4

-2

0

2

4

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10

Endo

Exo

Flujodecalor(mW)


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SEM


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During the course of nanotechnology-related

projects, we have built the necessary

infrastructure for the continuation of such

projects. We have also been expanding ourinfrastructure to solve problems related to

applications that we have been explored.

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We have designed andimplemented pilot plants to

produce NPs (silver and

TiO2:Ag


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Clinical and Laboratory Standards Institute (CLSI), M2-A9 and M7-A7, 2006NCCLS, Susceptibility Testing of Mycobacteria, Nocardiae and Other Aerobic Actinomycetes, M24-A,2003

National Committee for Clinical Laboratory Standar (NCCLS), M27-A, 1997

FACTOR CONDITIONMicroorganism Overnight culture

QuantificationSpectrometric, bacteria 620 nm

and fungus 530 nm, 0.5

McFarland

Culture media

Bacteria grown in Mueller

Hinton Broth and fungus grown

in RPMI

Endpoints

Determination of Minimum

Bactericidal Concentration

(MBC)

All experiments in triplicates
http://www.accugenlabs.com/mic.htmlhttp://www.accugenlabs.com/mic.htmlhttp://www.accugenlabs.com/mic.htmlhttp://www.accugenlabs.com/mic.htmlhttp://www.accugenlabs.com/mic.htmlhttp://www.accugenlabs.com/mic.htmlhttp://www.accugenlabs.com/mic.html


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b) 50 L Nanoparticle d)50 L microorganism

a) 50 L MH

BLACK

MICRODILUTION PLATE

3 4 5 6 7 9 10 11 12821

GREY

WHITE

BROWN

c)Dilutions


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MBC: Minimum Bactericidal Concentration test

MFC: Minimum Fungicidal Concentration test

PC

0.105 mg/ mL

0.21 mg/ mL

0.42 mg/ mL6.25 mg/ mL 12.5 mg/ mL

25 mg/ mL

MFCMBC

MBC and MFC are the lowest concentrtion of nanoparticles that kills 99.9% of the bacteria or fungi.

Clinical and Laboratory Standards Institute (CLSI), M2-A9 and M7-A7, 2006NCCLS, Susceptibility Testing of Mycobacteria, Nocardiae and Other Aerobic Actinomycetes, M24-A,2003National Committee for Clinical Laboratory Standar (NCCLS), M27-A, 1997


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S.aureusMRSAE.coliP.aeruginosaA.baumanii

M.smegmatisB.subtilisC.albicans

Minimum Bactericidal Concentration (MBC)

10m L

MBC

MIC
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This is the disc diffusion method (ZOI,Kirby Bauer)Used to demonstrate activity / potency

of antimicrobials or antibiotics by

measuring the zone of inhibition against

specified microorganisms. The test is

applicable for materials treated with an

antimicrobial agent that leaches out of

the material.
http://www.accugenlabs.com/zone-of-inhibition-kirby-bauer.htmlhttp://www.accugenlabs.com/zone-of-inhibition-kirby-bauer.htmlhttp://www.accugenlabs.com/zone-of-inhibition-kirby-bauer.htmlhttp://www.accugenlabs.com/zone-of-inhibition-kirby-bauer.htmlhttp://www.accugenlabs.com/zone-of-inhibition-kirby-bauer.htmlhttp://www.accugenlabs.com/zone-of-inhibition-kirby-bauer.htmlhttp://www.accugenlabs.com/zone-of-inhibition-kirby-bauer.htmlhttp://www.accugenlabs.com/zone-of-inhibition-kirby-bauer.htmlhttp://www.accugenlabs.com/jis-z-2801-antimicrobial-testing-laboratories.htmlhttp://www.accugenlabs.com/jis-z-2801-antimicrobial-testing-laboratories.htmlhttp://www.accugenlabs.com/jis-z-2801-antimicrobial-testing-laboratories.htmlhttp://www.accugenlabs.com/jis-z-2801-antimicrobial-testing-laboratories.html


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In these last years we have participated with

different companies trying to implement

applications based on nanotechnology

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Previousprojects


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Working with industries in applied research is

not easy

They have different rhythm of work

They do not want to deviate from the originalobjectives

They are not interested in publishing results

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Ceramics WC units, tiles and joint fillers for tiles

Plastics

Paints

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Synthesis methods for low cost materials

Scaling up

Aging/stabilization of nanoparticles

oxidation and/or fragmen

tation Environmental implications of nanoparticles

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Bio assays

Antimicrobial

Cytotoxicity

Antimicrobial mechanism ofnanoparticles (metallic)

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Biofilm inhibition


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The ceramic industry in Mexico is a very

important sector and is currently seeking for

innovative products.

Especially they are looking for ceramicsurfaces with different functionalities

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Sanitarios Lamosa

is a company dedicated to the design, manufacturingand commercialization of ceramic bathroom

fittings, oriented on the residential and institutional

use markets.

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Explorative project with a large Mexican paint

maker company:

Evaluating commercial additives

Adapting and validating microbiological assays

for paints

Testing inorganic additives (nanoparticles) Designing organic/inorganic additives


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Explorative project with a large Mexican paint

maker company:

Evaluating commercial additives Adapting and validating microbiological assays

for paints

Testing inorganic additives (nanoparticles) Designing organic/inorganic additives

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Sntesis de lasnanopartculas

Mtodo dereduccin

Caracterizacin

DRX, MET,DDL, UV-Vis Pruebas

Bactericidas

Mtodo demicrodilucin

en placa

Elaboracin delas pinturas

Conincorporacin de Nps

PruebasBactericidas

Protocolo delas placaspetrifilm 3M Pruebas

colorimtricas

Desviacindel color(Espacio decolorCIEL*a*b*)


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Pintura NanopartculasPintura conefecto

bacteriosttico


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a)

b)

c)

d)

e)

f)

Resultados de placa petrifilm de un muestreo sobre superficies: a), b), c), d) es el

recuento de bacterias; a) mesa y b) pared de cafetera, c) piso y d) lavabo de un

bao, e) y f) corresponde al recuento de hongos del piso y el lavabo

respectivamente.


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Resultados de concentracin inhibitoria mnima de nanopartculas de

compsitos TiO2@Ag.

Concentracin inhibitoria mnima (mg mL-1)Bacteria

Muestra

S. aureus (R) E. coli

Concentracin del

compsito

Concentracin de

Ag en el compsito

Concentracin del

compsito

Concentracin de

Ag en el compsito

1_1 ----* No ----* No

2_2 ----* No ----* NoNanopartculas de Ag No 16.67 No 13.02

M2_ 1, 10:1 333.33 129.10 33.10 354.17 166.15 35.16

MS_1@Ag 2, 10:1 375.00 136.93 28.12 395.83 173.59 29.68

M!, 25:1 250.00 0.0000 1.250 135.42 61.45 0.68

M2 2,2 5:1 270.00 122.90 1.080 208.33 64.55 0.83

M1 14 50:1 400.00 136.93 1.40 333.33 129.10 1.13

M 2 50:1 416.67 129.10 0.86 375.00 136.93 0.86

M 1 10:1 208.33

64.55 20.68 187.50

68.47 18.61M 2 10:1 229.17 51.03 19.25 187.50 68.47 15.75

M 14 25:1 291.67 102.06 1.46 166.67 64.55 0.83

M 2 25:1 291.67 102.06 1.46 270.83 122.90 1.35

M 14 50:1 416.67 129.10 1.42 458.33 102.06 1.56

M 2 50:1 416.67 129.10 1.29 458.33 102.06 1.42

datos para cada bacteria: CIM promedio del compsito (mg/mL) y la desviacin estndar (primeracolumna) y concentracin de Ag en el compsito (mg/mL) (segunda columna).

* En estas muestras no se encontr actividad bactericida a las concentraciones experimentadas.

Pruebas bactericidas sobre superficies de pintura


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Pruebas bactericidas sobre superficies de pintura

Muestra Efectividad (%)Pintura formulada (sin NP2) 64.48 7.61Pintura formulada (Sin Np2) 67.56 5.67

Pintura + Nanopartculas de Ag 66.02 2.66Pintura + M 1 10:1 93.44 1.72Pintura + M 125:1 99.11 0.84

n = 6

Resultados de pruebas bactericidas sobre superficies pintadas, utilizando

placas Petrifilm.


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ts

(4) Centro de Investigacin en Quimica Aplicada, Mxico

(5) Centro de Investigacin en Materiales Avanzados, S.C., Mxico

(6) SO.F.TER S.p.A., Italy

(7) Universidad Autnoma de San Luis Potosi, Mxico

(8) SO.VE.RE. S.p.A., Italy

(9) Owens Corning Mexico S.de R.L.de C.V., Mxico

(10) Owens Corning Fiberglas A.S. Ltda, Brazil


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Proceso de incorporacin de

nanopartculas en la matriz polimrica;

47.62 g/g (microgramos de

nanopartculas de plata por gramo de

poliestireno =0.0048% en peso


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Proceso de extrusin y peletizado del

nanocompsito; a) perfil de temperatura de

extrusin

Probetas de evaluacin para pruebas

mecnicas y antimicrobianas


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UFC %Inhibicin R Confiabilidad

OM07 (Testigo) 4.27E+06

OM08 6.67E+04 98.4% 1.81 0.02

BF09 9.33E+04 97.3% 1.58 0.01

BF10 3.33E+04 99.2% 2.11 0.01

BF11 0.00E+00 100% --- ---

R: Reduccin logartmica, UFC: Unidades Formadoras de Colonias

NOTA:


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We are still at the learning stage

Conventional Industries (cement, plastics, paints, textiles manufacturers)

are demanding application based in nanotechnology

In Mexico the nanotechnology is a field of opportunities

Documents

antimicrobial surfaces